Article
Microbiology
Xue-Lian Luo, Xiu-Dan Zhang, Bei-Jie Li, Tian Qin, Zhi-Jie Cao, Qian-Jin Fan, Jing Yang, Dong Jin, Shan Lu, Ya-Yun Zheng, Xue-Fang Xu, Ji Pu, Jianguo Xu
Summary: Lassa virus is an important human pathogen causing hemorrhagic fever in West Africa, with an increasing risk of imported cases to other countries due to increased traveling. The high nucleotide diversity of the virus strains complicates the development of diagnostic assays. The GPC RT-PCR/2007 assay and the Mabsky kit showed good sensitivity and specificity for detecting most LASV strains.
MICROBIOLOGY SPECTRUM
(2023)
Article
Infectious Diseases
Georgi Tomov, Nikola Stamenov, Deyan Neychev, Kiril Atliev
Summary: This study aimed to examine the possible association between tongue piercing and Candida spp. infection in healthy individuals. The results suggest that the main risk factors for tongue colonization with Candida spp. are gender, smoking, and improper hygiene, while tongue piercing is not a risk factor for colonization of Candida spp.
Article
Immunology
Danna Zhou, Shuangshuang Wang, Keli Yang, Xue Liu, Wei Liu, Rui Guo, Wan Liang, Fangyan Yuan, Zewen Liu, Ting Gao, Yong-xiang Tian
Summary: A new real-time nucleic acid sequence-based amplification (RT-NASBA) method was developed for rapid detection of Japanese encephalitis virus (JEV), showing high specificity and sensitivity.
MICROBIAL PATHOGENESIS
(2021)
Article
Immunology
Gwynngelle A. Borillo, Ron M. Kagan, Elizabeth M. Marlowe
Summary: This study demonstrates that multiplexed real-time RT-PCR genotyping has strong agreement with whole-genome sequencing (WGS) and may provide additional SARS-CoV-2 variant screening capabilities when WGS is unavailable or cost-prohibitive. RT-PCR genotyping assays can also supplement existing sequencing efforts, providing rapid results at or near the time of diagnosis to guide patient management.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Food Science & Technology
Wajeehah Wajahat, Z. R. Azaz Ahmad Azad, Sadaf Nazir, Gazia Nasir
Summary: The study evaluated the use of RT-PCR to detect adulteration of camel milk, successfully amplifying camel specific primers. The results indicated positive amplification for camel milk and its products, demonstrating the effectiveness and practicality of this method for identification.
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
(2022)
Article
Microbiology
G. Vidanapathirana, A. L. S. K. Angulmaduwa, T. S. Munasinghe, E. W. M. A. Ekanayake, P. Harasgama, S. T. Kudagammana, B. N. Dissanayake, L. V. C. Liyanapathirana
Summary: The colonization rate of pneumococci was higher when detected using RT-PCR compared to culture, although it was lower in healthy children. The pneumococcal colonization density in children with respiratory symptoms was higher, but not significantly different compared to previous studies. Further evaluation of RT-PCR for pneumococcal detection is needed.
Article
Microbiology
David J. Alouani, Roshani R. P. Rajapaksha, Mehul Jani, Daniel D. Rhoads, Navid Sadri
Summary: This study introduces a novel deep learning approach to interpret RT-PCR data, with the developed model serving as a quality assurance tool for real-time monitoring of result interpretation, applicable to pathogen detection such as SARS-CoV-2.
JOURNAL OF CLINICAL MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Peter Braun, Martin Duy-Thanh Nguyen, Mathias C. Walter, Gregor Grass
Summary: A real-time PCR assay targeting a specific single nucleotide polymorphism in Bacillus anthracis genomes was validated, showing high specificity, linearity, and sensitivity. Adapting the assay for reverse transcription PCR on 16S rRNA transcripts significantly increased sensitivity compared to DNA-targeting assays, offering great potential for molecular diagnostics.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Public, Environmental & Occupational Health
V Caramello, A. Macciotta, A. De Salve, A. Mussa, A. M. De Leo, F. Bar, D. Panno, F. Nota, C. Y. G. Ling, F. Solitro, F. Ricceri, C. Sacerdote, A. Boccuzzi
Summary: This study aimed to compare patients who initially tested negative for COVID-19 with those who tested positive in terms of clinical presentation, laboratory results, imaging findings, and prognosis. Results showed that patients with negative initial tests tended to have more comorbidities such as heart disease, immunosuppression, and diabetes, and imaging was useful in identifying them. True positive patients had a higher risk of serious complications.
Article
Virology
Brigitte Sigrist, Jessica Geers, Sarah Albini, Dennis Rubbenstroth, Nina Wolfrum
Summary: Avian bornaviruses were first identified as causative agents of a disease in parrots in 2008. Currently, detection of these viruses is primarily done through RT-PCR, but existing methods have limitations in specificity and breadth of detection. A new multiplex real-time RT-PCR assay has been developed to overcome these limitations and simultaneously detect and differentiate avian bornaviruses.
Article
Plant Sciences
Scott D. Anderson, Cynthia A. Gleason
Summary: Root-knot nematodes, especially Meloidogyne chitwoodi, pose a significant threat to potato production in the Northwestern region of the USA. Recently, two unfamiliar Meloidogyne species, M. fallax and M. minor, were discovered on golf turfgrasses, raising concerns about the potential infestation of potato fields. To address this issue, a molecular beacon real-time PCR assay was developed to accurately detect and differentiate M. chitwoodi, M. fallax, and M. minor from nematode extracts.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biochemical Research Methods
Zeinab Alsaheli, Ali Abdallah, Ornella Incerti, Ahmad Shalaby, Sahar Youssef, Michele Digiaro, Toufic Elbeaino
Summary: Singleplex and multiplex real-time RT-PCR assays were developed to detect seven fig-infecting viruses, and a large-scale survey in Egypt showed a high prevalence of mixed infections, with FMV being the most commonly observed virus.
JOURNAL OF VIROLOGICAL METHODS
(2021)
Article
Chemistry, Multidisciplinary
Dulguunnaran Naranbat, Lindsay Schneider, Rami Kantor, Curt G. Beckwith, Lauri Bazerman, Fizza Gillani, Sujata Sahu, Kim Rapoza, Soya Sam, Vlad Novitsky, Jimin Shin, Evelyn Hipolito, Isabella Diaz, Daniella Carnevale, Anubhav Tripathi
Summary: COVID-19 is a global pandemic that requires efficient diagnostic tools. This preliminary study evaluated a novel SARS-CoV-2 diagnostic test that does not require viral transport medium or viral RNA extraction, reducing sample preparation time and the need for costly reagents. Further validation is needed.
Article
Public, Environmental & Occupational Health
Daniel F. F. Escobar, Pablo Diaz, Diego Diaz-Dinamarca, Rodrigo Puentes, Pedro Alarcon, Barbara Alarcon, Ivan Rodriguez, Ricardo A. Manzo, Daniel A. Soto, Liliana Lamperti, Janepsy Diaz, Heriberto E. Garcia-Escorza, Abel E. Vasquez
Summary: The Chilean city of Concepcion experienced a second wave of COVID-19 in January and the whole country faced the same situation in early April 2021. A study conducted during total quarantine in Concepcion validated a highly sensitive and specific RT-PCR method using saliva specimens, showing comparable performance to NPS specimens. This modified method provides a real testing alternative to health authorities and local laboratories in Chile.
FRONTIERS IN PUBLIC HEALTH
(2021)
Article
Biochemical Research Methods
Giovanni Faggioni, Riccardo De Santis, Filippo Moramarco, Mario Di Donato, Angelo De Domenico, Filippo Molinari, Giancarlo Petralito, Claudia Fortuna, Giulietta Venturi, Giovanni Rezza, Florigio Lista
Summary: Yellow fever is a viral disease that can cause severe hemorrhage, but can be controlled through mass immunization campaigns with a safe and effective vaccine. However, the re-emergence of the yellow fever virus since the 1960s requires rapid and specific viral detection methods for timely control measures. A novel molecular assay with high sensitivity and specificity in detecting yellow fever virus strains has been developed.
JOURNAL OF VIROLOGICAL METHODS
(2023)
Article
Chemistry, Analytical
Un-Jung Kim, Byoung Chan Kim
SENSORS AND ACTUATORS B-CHEMICAL
(2017)
Article
Chemistry, Analytical
Dung T. Nguyen, Hye Ri Kim, Jae Hee Jung, Kang-Bong Lee, Byoung Chan Kim
SENSORS AND ACTUATORS B-CHEMICAL
(2018)
Article
Chemistry, Physical
Byoung Chan Kim, Eunhoo Jeong, Eunju Kim, Seok Won Hong
APPLIED CATALYSIS B-ENVIRONMENTAL
(2019)
Article
Chemistry, Analytical
Muthusamy Selvaraj, Kanagaraj Rajalakshmi, Yun-Sik Nam, Yeonhee Lee, Byoung Chan Kim, Sung Jin Pai, Sang Soo Han, Kang-Bong Lee
JOURNAL OF ANALYTICAL METHODS IN CHEMISTRY
(2019)
Article
Biochemical Research Methods
Hye Ri Kim, Min Yong Song, Byoung Chan Kim
ANALYTICAL BIOCHEMISTRY
(2020)
Article
Chemistry, Multidisciplinary
Yu Sung Cho, Hye Ri Kim, Hyun Sik Ko, Sang Bin Jeong, Byoung Chan Kim, Jae Hee Jung
Article
Chemistry, Physical
Waleed Ahmad, Eunseuk Park, Heehyeon Lee, Jin Young Kim, Byoung Chan Kim, Jongsoo Jurng, Youngtak Oh
Summary: Synthesizing defective anatase TiO2 through chemical vapor condensation and impregnating with Pt resulted in a Pt/CVC-TiO2 catalyst with high efficiency in removing HCHO at room temperature, demonstrating good stability over time. The study highlighted the synergistic relationship between oxygen vacancies in the TiO2 support and the resulting HCHO oxidation functionality.
APPLIED SURFACE SCIENCE
(2021)
Article
Chemistry, Analytical
Ho-Kyeong Kim, Hye-Ri Kim, Su-Jin Yoon, Kang-Bong Lee, Jungbae Kim, Byoung-Chan Kim
Summary: In this study, specific aptamers for detecting B. carboniphilus were isolated and a two-stage label-free aptasensing platform was established. The platform utilizes colorimetric detection to quantify bacteria, showing reliable performance with linear correlation and a low detection limit.
Article
Environmental Sciences
Ho Kyeong Kim, Phuong Thy Nguyen, Moon Il Kim, Byoung Chan Kim
Summary: Mercury emissions are increasing globally due to industrialization, and its persistence and bioaccumulation can severely affect human health. Hybrid nanoflowers were synthesized for Hg adsorption and elimination from solutions, with the functionalized Apt-Ag-MNP-PDA-Cu NFs showing significantly improved performance in Hg removal. The results suggest that the Apt-Ag-MNP-PDA-Cu NFs could be an efficient Hg-removing adsorbent by providing binding sites for the formation of T-Hg2+-T complexes.
Article
Biochemical Research Methods
In Hwa Jeong, Ho Kyeong Kim, Hye Ri Kim, Jungbae Kim, Byoung Chan Kim
Summary: In this study, an aptamer CB-5 that can specifically bind to C. braakii was rapidly isolated using a centrifugation-based partitioning method. The CB-5 aptamer showed high binding affinity and specificity for C. braakii, indicating its potential use as a reliable diagnostic receptor for airborne bacteria.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Hye Ri Kim, Byoung Chan Kim
Summary: In this study, a newly proposed sequential partitioning method was used to isolate specific aptamers for all seven species of Cronobacter. This method avoided repeated enrichment steps and reduced the selection time compared to the conventional SELEX process. Four aptamers with high affinity and specificity for Cronobacter were successfully isolated, and they could effectively detect Cronobacter in contaminated PIF.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Oleksandr Tsymbalenko, Soyoung Lee, Yong-Min Lee, Yun-Sik Nam, Byoung Chan Kim, Jin Young Kim, Kang-Bong Lee
Summary: A highly sensitive and selective NH3 gas sensor was developed by doping single-layer pristine graphene with copper(II) oxide (CuO) nanoparticles. CuO nanoparticles of approximately 15 nm were synthesized and doped onto graphene using a simple spin coating method. NH3 gas reacts with the oxygen species on the graphene surface, causing a change in graphene's valence channel and resistivity. The sensor exhibited a resistivity response of approximately 83% and fast response and recovery times.
Correction
Chemistry, Analytical
Hye Ri Kim, Myunghee Kim, Byoung Chan Kim
Article
Chemistry, Analytical
Hye Ri Kim, Myunghee Kim, Byoung Chan Kim
Summary: In this study, C. sakazakii-specific aptamers were isolated using a centrifugation-based partitioning method, which showed high affinity and specificity for the pathogen. Among the two aptamers isolated, SC25 displayed efficient detection of C. sakazakii in powdered infant formula with less cross-reactivity, potentially reducing overall testing time compared to traditional methods.
Article
Engineering, Environmental
Sung-Gil Hong, Byoung Chan Kim, Hyon Bin Na, Jinwoo Lee, Jongkyu Youn, Seung-Wook Chung, Chang-Won Lee, Byoungsoo Lee, Han Sol Kim, Erik Hsiao, Seong H. Kim, Byung-Gee Kim, Hyun Gyu Park, Ho Nam Chang, Taeghwan Hyeon, Jonathan S. Dordick, Jay W. Grate, Jungbae Kim
CHEMICAL ENGINEERING JOURNAL
(2017)