Molecular engineering of industrial enzymes: recent advances and future prospects

Rational Design of a Novel Propeptide for Improving Active Production of Streptomyces griseus Trypsin in Pichia pastoris

(2013) Zhenmin Ling et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Fusion of an Oligopeptide to the N Terminus of an Alkaline α-Amylase from Alkalimonas amylolytica Simultaneously Improves the Enzyme's Catalytic Efficiency, Thermal Stability, and Resistance to Oxidation

(2013) Haiquan Yang et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Biochemical Diversity of Carboxyl Esterases and Lipases from Lake Arreo (Spain): a Metagenomic Approach

(2013) Mónica Martínez-Martínez et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Enhanced thermal stability and specific activity of Pseudomonas aeruginosa lipoxygenase by fusing with self-assembling amphipathic peptides

(2013) Xinyao Lu et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Engineering of a bacterial tyrosinase for improved catalytic efficiency towards D-tyrosine using random and site directed mutagenesis approaches

(2013) Susan Molloy et al.   BIOTECHNOLOGY AND BIOENGINEERING

Site-directed Mutagenesis of Cysteine Residues in Phi-class Glutathione S-transferase F3 from Oryza sativa

(2013) Hyun-Joo Jo et al.   BULLETIN OF THE KOREAN CHEMICAL SOCIETY

The Structure of aStreptomyces avermitilisα-l-Rhamnosidase Reveals a Novel Carbohydrate-binding Module CBM67 within the Six-domain Arrangement

(2013) Zui Fujimoto et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Relationship between Escherichia coli AppA phytase's thermostability and salt bridges

(2013) Baojin Fei et al.   JOURNAL OF BIOSCIENCE AND BIOENGINEERING

Improved activity and thermostability of Bacillus pumilus lipase by directed evolution

(2013) Nagihan Akbulut et al.   JOURNAL OF BIOTECHNOLOGY

Thermal stability engineering of Glomerella cingulata cutinase

(2013) I.-S. Chin et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Characterisation of a thermo-alkali-stable lipase from oil-contaminated soil using a metagenomic approach

(2013) Jianhua Zheng et al.   SYSTEMATIC AND APPLIED MICROBIOLOGY

Directed evolution: Past, present, and future

(2012) Ryan E. Cobb et al.   AICHE JOURNAL

Structure-Based Engineering of Methionine Residues in the Catalytic Cores of Alkaline Amylase from Alkalimonas amylolytica for Improved Oxidative Stability

(2012) Haiquan Yang et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Compensation of the enantioselectivity-activity trade-off in the directed evolution of an esterase from Rhodobacter sphaeroides by site-directed saturation mutagenesis

(2012) Fei Guo et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Combinatorial approach of statistical optimization and mutagenesis for improved production of acidic phytase by Aspergillus niger NCIM 563 under submerged fermentation condition

(2012) K. Bhavsar et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Combination of site-directed mutagenesis and calcium ion addition for enhanced production of thermostable MBP-fused heparinase I in recombinant Escherichia coli

(2012) Shuo Chen et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Engineering of LadA for enhanced hexadecane oxidation using random- and site-directed mutagenesis

(2012) Yanpeng Dong et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Improving the thermostability of alpha-amylase by combinatorial coevolving-site saturation mutagenesis

(2012) Chenghua Wang et al.   BMC BIOINFORMATICS

Many Pathways in Laboratory Evolution Can Lead to Improved Enzymes: How to Escape from Local Minima

(2012) Yosephine Gumulya et al.   CHEMBIOCHEM

The Extracellular Protein Factor Epf fromStreptococcus pyogenesIs a Cell Surface Adhesin That Binds to Cells through an N-terminal Domain Containing a Carbohydrate-binding Module

(2012) Christian Linke et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

LOXPsa1, the first recombinant lipoxygenase from a basidiomycete fungus

(2012) Ina Plagemann et al.   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

Directed evolution as a powerful synthetic biology tool

(2012) Ryan E. Cobb et al.   METHODS

Rational design and directed evolution of a bacterial-type glutaminyl-tRNA synthetase precursor

(2012) L.-T. Guo et al.   NUCLEIC ACIDS RESEARCH

Rational design of a Bacillus circulans xylanase by introducing charged residue to shift the pH optimum

(2012) Subarna Pokhrel et al.   PROCESS BIOCHEMISTRY

Deletion combined with saturation mutagenesis of N-terminal residues in transglutaminase from Streptomyces hygroscopicus results in enhanced activity and thermostability

(2012) Kangkang Chen et al.   PROCESS BIOCHEMISTRY

Artificial proteins from combinatorial approaches

(2012) Agathe Urvoas et al.   TRENDS IN BIOTECHNOLOGY

Increased thermostability of microbial transglutaminase by combination of several hot spots evolved by random and saturation mutagenesis

(2011) Karin Buettner et al.   AMINO ACIDS

Truncation of N- and C-terminal regions of Streptococcus mutans dextranase enhances catalytic activity

(2011) Young-Min Kim et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Microbial xylanases: Engineering, production and industrial applications

(2011) Veeresh Juturu et al.   BIOTECHNOLOGY ADVANCES

Heterologous expression, biochemical characterization, and overproduction of alkaline α-amylase from Bacillus alcalophilus in Bacillus subtilis

(2011) Haiquan Yang et al.   Microbial Cell Factories

Characterization of Thermobifida fusca Cutinase-Carbohydrate-Binding Module Fusion Proteins and Their Potential Application in Bioscouring

(2010) Y. Zhang et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Screening for improved activity of a transglutaminase from Streptomyces mobaraensis created by a novel rational mutagenesis and random mutagenesis

(2010) Keiichi Yokoyama et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Optimizing non-natural protein function with directed evolution

(2010) Eric M Brustad et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Iterative Saturation Mutagenesis Accelerates Laboratory Evolution of Enzyme Stereoselectivity: Rigorous Comparison with Traditional Methods

(2010) Manfred T. Reetz et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Expression, purification, and characterization of proteins from high-quality combinatorial libraries of the mammalian calmodulin central linker

(2010) Luke H. Bradley et al.   PROTEIN EXPRESSION AND PURIFICATION

Directed Evolution of an Enantioselective Enoate-Reductase: Testing the Utility of Iterative Saturation Mutagenesis

(2009) Despina J. Bougioukou et al.   ADVANCED SYNTHESIS & CATALYSIS

Enhancement of cell viability and alkaline polygalacturonate lyase production by sorbitol co-feeding with methanol in Pichia pastoris fermentation

(2009) Zhihao Wang et al.   BIORESOURCE TECHNOLOGY

Directed evolution: new parts and optimized function

(2009) Michael J Dougherty et al.   CURRENT OPINION IN BIOTECHNOLOGY

The carbohydrate-binding module family 20 - diversity, structure, and function

(2009) Camilla Christiansen et al.   FEBS Journal

Truncation of the cellulose binding domain improved thermal stability of endo-β-1,4-glucanase from Bacillus subtilis JA18

(2008) Yujuan Wang et al.   BIORESOURCE TECHNOLOGY

Addressing the Numbers Problem in Directed Evolution

(2008) Manfred T. Reetz et al.   CHEMBIOCHEM

Site-directed mutagenesis and CBM engineering of Cel5A (Thermotoga maritima)

(2008) Shobana Arumugam Mahadevan et al.   FEMS MICROBIOLOGY LETTERS

Improving activity and stability of cutinase towards the anionic detergent AOT by complete saturation mutagenesis

(2008) V. Brissos et al.   PROTEIN ENGINEERING DESIGN & SELECTION