Article
Biochemistry & Molecular Biology
Yanyan Wang, Yang Wang, Hefei Bai, Yuqian Han, Fang Yu
Summary: Camptothecin and its derivatives have antitumor effects and can be induced by exogenous abscisic acid (ABA) in Camptotheca acuminata. The study identifies an ABA transporter, CaABAT, which is predominately expressed in leaves and plays a role in regulating ABA import and subsequent changes in physiological status. Silencing of CaABAT down-regulates ABA response genes and decreases expression of CPT biosynthesis pathway genes, leading to low accumulation levels of CPT.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Xiang Pu, Hu-Chuan Gao, Min-Ji Wang, Jia-Hua Zhang, Jia-Heng Shan, Meng-Han Chen, Li Zhang, Han-Guang Wang, An-Xiang Wen, Ying-Gang Luo, Qian-Ming Huang
Summary: Treatments with abiotic elicitors can effectively promote the accumulation of specialized metabolites in plants. This study comprehensively analyzed the elicitation effects of MeJa, AgNO3, and PEG on the biosynthesis of camptothecin (CPT) in Camptotheca acuminata plantlets. The treatments inhibited the photosynthetic pathway and stimulated carbon metabolism and secondary metabolic pathways, resulting in increased CPT levels. The study also discovered new alkaloids, CPT analogs, and precursors, as well as identified key genes involved in CPT biosynthesis.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Chemistry, Applied
Yanni Sun, Ning Zhang, Cuiling Wang, Xiao Bai, Yahui Wei, Jianli Liu
Summary: Camptotheca acuminata seedlings catalyze the conversion of exogenous camptothecin into 10-hydroxycamptothecin and 9-methoxycamptothecin, offering a new method for synthesizing these two camptothecin derivatives.
NATURAL PRODUCT RESEARCH
(2021)
Article
Agricultural Engineering
Xiaoxuan Fan, Xinting Lin, Qingyan Ruan, Jingyi Wang, Yinkai Yang, Miaomiao Sheng, Wei Zhou, Guoyin Kai, Xiaolong Hao
Summary: This review summarizes the anti-tumor mechanisms and applications of camptothecin drugs, the extraction of camptothecin, the biosynthetic genes of camptothecin and its derivatives in C. acuminata, and prospects the application of camptothecin metabolic engineering in CPT-producing plants.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Plant Sciences
Yake Yi, Yaqiong Peng, Tao Song, Siqiong Lu, Zhenning Teng, Qin Zheng, Fankai Zhao, Shuan Meng, Bohang Liu, Yan Peng, Guanghui Chen, Jianhua Zhang, Nenghui Ye
Summary: This study reveals that NR-associated NO plays a crucial role in improving salt tolerance during seed germination in rice by regulating ABA catabolism. Key factors in this mechanism include nitrate, NLP2 transcription factor, and OsABA8ox1, a target gene for NLP2.
Article
Agricultural Engineering
Siying Wang, Lingling Wang, Chaofan Sun, Lanlan Xie, Wenqiang Jia, Dongmei Zhao, Xiuhua Zhao
Summary: As countries focus more on environmental issues, the development of sustainable green technologies has gained attention. This study proposes a green, low-energy-consumption and high-efficiency extraction method for insoluble alkaloids from plants at room temperature. The method utilizes ultra-high pressure assisted micellar media and has shown high efficiency and low energy consumption for the extraction of active components.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Article
Plant Sciences
Anamika Paul, Anik Sarkar, Krishnendu Acharya, Nilanjan Chakraborty
Summary: In this study, a fungal elicitor was used to induce defense responses in Catharanthus roseus. Results showed that the fungal elicitor was able to induce defense-related enzymes and the signaling molecule nitric oxide, and also exhibited protective effects against Alternaria leaf blight disease in C. roseus.
JOURNAL OF PLANT GROWTH REGULATION
(2023)
Article
Plant Sciences
Pariya Pirooz, Rayhaneh Amooaghaie, Alimohammad Ahadi, Fariba Sharififar
Summary: The study demonstrated that under copper stress, silicon and nitric oxide help enhance the resistance of Salvia officinalis leaves and roots, by reducing copper accumulation, increasing proline content, enhancing antioxidant enzyme activity, and mitigating lipid peroxidation and H2O2 accumulation.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2021)
Article
Microbiology
Luca Freddi, Jorge A. de la Garza-garcia, Sascha Al Dahouk, Alessandra Occhialini, Stephan Koehler
Summary: This study reveals that atypical Brucella species can grow under anoxic conditions strictly dependent on nitrate, while classical species exhibit different behavior. Atypical Brucella species have a more efficient denitrification pathway and rapid consumption of nitrite, which may be related to their ability to colonize different hosts and rapid growth.
MICROBIOLOGY SPECTRUM
(2023)
Article
Biochemistry & Molecular Biology
Samuel J. Gentle, Khandaker A. Ahmed, Nengjun Yi, Casey D. Morrow, Namasivayam Ambalavanan, Charitharth Lal, Rakesh P. Patel
Summary: The study found that nitrate reductase activity in the oral cavity peaked at 29 weeks' post menstrual age, and infants who developed bronchopulmonary dysplasia (BPD) had significantly lower nitrate reductase activity at this time. The oral microbiota and nitrate reductase activity may play a role in the development of BPD in extremely preterm infants.
Article
Biochemistry & Molecular Biology
Pedro J. Pacheco, Juan J. Cabrera, Andrea Jimenez-Leiva, Eulogio J. Bedmar, Socorro Mesa, German Tortosa, Maria J. Delgado
Summary: This study provides a comprehensive investigation of the role of copper (Cu) in denitrification of Bradyrhizobium diazoefficiens. It reveals that Cu influences the expression and protein levels of denitrification-related genes, and also demonstrates a post-transcriptional regulation. The findings of this study are important for reducing N2O emissions from agricultural soils.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Justin C. Miller, Allison J. Hollatz, Mary A. Schuler
Summary: The study identifies differences in the terpene indole alkaloid (TIA) pathways in Catharanthus roseus and Camptotheca acuminata, and demonstrates the ability of specific P450 proteins to convert certain compounds in these pathways. The research provides valuable tools for further examination of substrate recognition and the unique mechanism of C-C bond scission involved in these pathways.
Article
Plant Sciences
Quang Tri Le, Won Je Lee, Jun Ho Choi, Dinh Thanh Nguyen, Hai An Truong, Sang-A Lee, Suk-Whan Hong, Hojoung Lee
Summary: The loss-of-function mutant of the transcription factor NLP7, which regulates the primary response to nitrate, exhibits salt tolerance and a balanced accumulation of nitrate anions and sodium cations. The mutant shows reduced stress response, lower levels of marker genes, and less accumulation of proline, nitric oxide, and reactive oxygen species under high salinity. However, it also accumulates more sodium ions in the shoots and has higher nitrate levels compared to the wild type. These findings suggest that targeting nitrate metabolism and signaling could improve salt tolerance.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Ritesh Kumar Raipuria, Sunita Kataria, Anshul Watts, Meeta Jain
Summary: The study suggests that nitric oxide (NO) may be the key signaling molecule in soybean seedlings' tolerance towards UV-B stress under the stimulation of static magnetic field (SMF), while NO synthase (NOS) may be responsible for SMF-induced NO production. The use of NO donor SNP can improve the parameters of seedlings, while inhibitors of NR, NOS, and DPI can reduce this improvement.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
(2021)
Article
Plant Sciences
Cengiz Kaya, Tahir Polat, Muhammad Ashraf, Prashant Kaushik, Mohammed Nasser Alyemeni, Parvaiz Ahmad
Summary: The study showed that glutathione (GSH) can reduce oxidative stress and tissue cadmium content, while improving growth, altering water relations, and increasing the activities of key enzymes in cadmium-stressed maize plants. Nitrate reductase (NR) may serve as a potential source of nitric oxide (NO) generated by GSH, accelerating the activities of antioxidant enzymes.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2021)