期刊
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
卷 89, 期 1, 页码 73-78出版社
SPRINGER
DOI: 10.1007/s00253-010-2868-9
关键词
L-Lactic acid; Lactobacillus casei; Osmotic stress resistance; High temperature
资金
- Program of Introducing Talents of Discipline to Universities [111-2-06]
- Program for Changjiang Scholars, and Innovative Research Team in University (PCSIRT) [IRT0532]
L-Lactic acid production by Lactobacillus casei was used as a model to study the mechanism of substrate inhibition and the strategy for enhancing L-lactic acid production. It was found that the concentration of cell growth and L-lactate decreased with the increase of glucose concentration and fermentation temperature. To enhance the osmotic stress resistance of the strain at high temperature, a mutant G-03 was screened and selected with 360 g/L glucose at 45 degrees C as the selective criterion. To further increase the cell growth for lactic acid production, 3 g/L of biotin was supplemented to the medium. As a result, L-lactate concentration by the mutant G-03 reached 198.2 g/L (productivity of 5.5 g L-1 h(-1)) at 41 degrees C in a 7-L fermentor with 210 g/L glucose as carbon source. L-Lactate concentration and productivity of mutant G-03 were 115.2% and 97.8% higher than those of the parent strain, respectively. The strategy for enhancing L-lactic acid production by increasing osmotic stress resistance at high temperature may provide an alternative approach to enhance organic acid production with other strains.
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