Article
Multidisciplinary Sciences
Gal Ofir, Ehud Herbst, Maya Baroz, Daniel Cohen, Adi Millman, Shany Doron, Nitzan Tal, Daniel B. A. Malheiro, Sergey Malitsky, Gil Amitai, Rotem Sorek
Summary: The TIR domain plays a crucial role in animal and plant immune systems, triggering cell death and participating in the bacterial antiviral defence system Thoeris. Research findings suggest that TIR-domain proteins in bacteria recognize infection and produce signalling molecules to execute cell death, similar to the roles of these proteins in plants.
Review
Microbiology
Liang Wu, Yubin Luo
Summary: The field of quorum sensing (QS) system is rapidly expanding our understanding of bacteria communication and social activities. Recent studies have shown that QS-related autoinducers play important roles in maintaining homeostasis in the gut microbiome and also in interactions between pathogens and their hosts under pathological conditions. This knowledge is crucial for developing novel antimicrobial therapeutic strategies.
FRONTIERS IN MICROBIOLOGY
(2021)
Review
Chemistry, Multidisciplinary
Jonas Kaltbeitzel, Peter R. Wich
Summary: Proteins and enzymes are versatile biomaterials with high specificity, degradability, low toxicity, and good biocompatibility, making them ideal for various medical applications. Protein nanoparticles, formed by arranging native or modified proteins into nanometer-sized assemblies, have emerged as a promising nanosystem for drug delivery, imaging, nanocatalysis, and protein therapy.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Review
Microbiology
Aadil H. Bhat, Minh Tan Nguyen, Asis Das, Hung Ton-That
Summary: This article summarizes the cell wall anchoring mechanism of surface proteins and pili in Gram-positive bacteria, as well as how sortase enzymes recognize substrates and the role of the cell wall sorting signal in modulating intramembrane signaling.
CURRENT OPINION IN MICROBIOLOGY
(2021)
Article
Biochemical Research Methods
Qi Liu, Chih-Yuan Hsu, Jia Li, Yu Shyr
Summary: This study developed a statistical method for examining dysregulated ligand-receptor interactions in different conditions, and successfully applied it to the study of important diseases such as COVID-19 infection and pulmonary fibrosis.
Editorial Material
Biochemistry & Molecular Biology
Mihe Hong, Yvonne Y. Chen
Summary: Researchers developed an in vitro model to study exhaustion of CAR-T cells and identified signatures of dysfunction, including transition to a NK-like phenotype. New gene targets were suggested to prevent exhaustion.
Review
Food Science & Technology
Yucheng Zhang, Ning Ma, Peng Tan, Xi Ma
Summary: This review provides an overview of how gut bacteria use quorum sensing (QS) to communicate and coordinate their activities, and the role of QS in the interaction between the gut host and microbiota. Understanding the communication network of gut microbiota is crucial for developing strategies against bacterial illnesses and improving gut health.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2022)
Article
Biochemistry & Molecular Biology
U. Pulliainen, C. Morandin, N. Bos, L. Sundstrom, E. Schultner
Summary: Through RNAseq analysis, it was found that social stimulation led to higher expression of sensory-related genes in larvae of the ant Formica fusca. These larvae expressed similar sensory-related genes as adult ants and larvae of other insects, including genes belonging to major insect chemosensory gene families. This study provides insights into the molecular changes associated with social information perception in social insect larvae.
INSECT MOLECULAR BIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Yanyan Miao, Qianqian Gao, Menghan Mao, Chao Zhang, Liqun Yang, Yang Yang, Da Han
Summary: This study describes a platform for the targeted degradation of membrane-associated proteins using bispecific aptamer chimeras, which efficiently shuttle therapeutically relevant membrane proteins to lysosomes for degradation. This method provides a universal platform for biochemical research and potential therapeutics using readily synthesized aptamer materials.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Biochemistry & Molecular Biology
Craig Sweet, Aayush Aayush, Logan Readnour, Kevin Solomon, David H. Thompson
Summary: A rapid purification workflow for ELP using polar organic solvent extraction and precipitation strategy was developed, providing highly pure ELP in approximately 2.5 hours while removing major host cell contaminants. This method can produce a functional ELP construct suitable for in vivo applications, demonstrating its efficiency and effectiveness in protein purification.
Article
Multidisciplinary Sciences
Gary Dixon, Heng Pan, Dapeng Yang, Bess P. Rosen, Therande Jashari, Nipun Verma, Julian Pulecio, Inbal Caspi, Kihyun Lee, Stephanie Stransky, Abigail Glezer, Chang Liu, Marco Rivas, Ritu Kumar, Yahui Lan, Ingrid Torregroza, Chuan He, Simone Sidoli, Todd Evans, Olivier Elemento, Danwei Huangfu
Summary: The previously unknown gene QSER1 plays a crucial role in regulating the methylation landscape, safeguarding bivalent promoters and poised enhancers of developmental genes, especially those in DNA methylation valleys. The genetic and biochemical interactions of QSER1 and TET1 support their cooperation in protecting transcriptional and developmental programs from de novo methylation mediated by DNMT3.
Article
Pharmacology & Pharmacy
Amani Elsayed, Nisrein Jaber, Mayyas Al-Remawi, Khalid Abu-Salah
Summary: Biopharmaceuticals, including enzymes, monoclonal antibodies, nucleic acids, and recombinant proteins, have become dominant in the pharmaceutical industry due to their safety and efficacy. However, their manufacturing faces challenges such as high cost, instability, and quality issues. This article provides an up-to-date review of the challenges encountered during different stages of biopharmaceutical production and discusses the solutions to these problems.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Multidisciplinary Sciences
Valentin Boichot, Franck Menetrier, Jean-Michel Saliou, Frederic Lirussi, Francis Canon, Mireille Folia, Jean-Marie Heydel, Thomas Hummel, Susanne Menzel, Maria Steinke, Stephan Hackenberg, Mathieu Schwartz, Fabrice Neiers
Summary: Oxidoreductases are crucial enzymes in xenobiotic metabolism and play a vital role in chemoprotection. They not only participate in the protection of chemosensory tissues but also affect chemosensory perception by modulating the activation pattern of chemosensory receptors. Aldehyde dehydrogenases and aldo-keto reductases, two types of oxidoreductases, act as the first line of defense against aldehyde compounds, including odorants. By analyzing human nasal mucus and epithelium samples, we identified the highly expressed oxidoreductases and demonstrated their strong enzymatic activity towards aldehyde odorants. The structure-function relationship was further supported by the X-ray structure resolution of human ALDH3A1 in complex with octanal.
SCIENTIFIC REPORTS
(2023)
Article
Biotechnology & Applied Microbiology
Nico Lingg, Monika Cserjan-Puschmann, Andreas Fischer, Petra Engele, Christina Kross, Rainer Schneider, Cecile Brocard, Matthias Berkemeyer, Gerald Striedner, Alois Jungbauer
Summary: The study demonstrated that cpCasp2 can be used to produce native proteins with authentic N-terminus through a fusion-protein process, leading to highly pure products with minimal contamination. This technology shows potential for industrial protein production and offers a scalable purification platform for future applications.
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
(2021)
Article
Oncology
Qian Zhang, Ying Jia, Pan Pan, Xiuping Zhang, Yanfei Jia, Ping Zhu, Xiaowei Chen, Yang Jiao, Guiyu Kang, Lulu Zhang, Xiaoli Ma
Summary: This study uncovers a new interaction between alpha 5-nAChR and Ly6E that inhibits cancer cell migration by modulating the TGF-beta 1/Smad signaling pathway in NSCLC.