Article
Microbiology
Yan Wu, Chun Tao Gu
Summary: This study re-evaluated the taxonomic status of Leuconostoc gelidum subsp. gasicomitatum using various methods and proposed rejecting the reclassification proposed by Rahkila et al.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2021)
Article
Food Science & Technology
So Yeong Mun, Ye Jin Seo, Hae Choon Chang
Summary: The potential psychrotrophic lactic acid bacterium, Leuconostoc gelidum subsp. aenigmaticum LS4, was investigated as a functional starter culture for food (vegetable or fruit) fermentation at low temperatures. This strain exhibited strong growth performance, antimicrobial activity, and improved food quality.
Article
Biochemistry & Molecular Biology
Klaudia Gustaw, Piotr Koper, Magdalena Polak-Berecka, Kamila Rachwal, Katarzyna Skrzypczak, Adam Wasko
Summary: The study describes a newly isolated strain Lactobacillus hilgardii FLUB from mead and characterizes its genome, highlighting its genetic repertoire and unique features. The strain shows a preference for fructose and exhibits resistance to high concentrations of alcohol and sugars, indicating its adaptation to the mead environment. Expertimental research simulating alcoholic beverage conditions confirms the niche specialization of L. hilgardii FLUB.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Food Science & Technology
Noam Shani, Simone Oberhaensli, Helene Berthoud, Remo S. Schmidt, Hans-Peter Bachmann
Summary: The study highlighted the importance of testing the antibiotic susceptibility of LDL strains to accurately assess their ABR status. It proposed adjusting the MCs for clinically-relevant antibiotics in order to improve the accuracy of phenotypic antibiotic testing.
Article
Microbiology
Justina Mileriene, Jurgita Aksomaitiene, Kristina Kondrotiene, Tora Asledottir, Gerd Elisabeth Vegarud, Loreta Serniene, Mindaugas Malakauskas
Summary: Safety is paramount in the food industry for any substance or microorganism used. Whole-genome sequencing confirmed that the indigenous dairy isolate LL16 is Lactococcus lactis subsp. lactis. It exhibited non-pathogenicity and the absence of genes associated with antimicrobial resistance, virulence, and biogenic amine formation. Additionally, it showed potential for producing bacteriocins and the neurotransmitter GABA, making it a functional strain suitable for the dairy sector.
Article
Biochemistry & Molecular Biology
Shuai Zhang, Dongyao Li, Xin Zhang, Yongsheng Sun, Sha Xu, Xinyu Wang, Na Zhang, Miaoshu Wang, Hongtao Tian, Chen Li
Summary: The study identified genes that persistently responded to acid during yogurt fermentation and were less affected by cold stress, suggesting their potential involvement in post-acidification. Among these genes, LDB_RS05285 was found to be crucial in inhibiting post-acidification and could be a new target for regulating yogurt quality.
Article
Entomology
Inga Bazukyan, Dimitrina Georgieva-Miteva, Tsvetelina Velikova, Svetoslav G. Dimov
Summary: This study reports the isolation of L. delbrueckii strain from the gut of a honeybee for the first time. Genomic analysis revealed that the strain possesses probiotic properties and has adapted to endosymbiotic conditions. This potentially endosymbiotic strain is capable of playing beneficial roles for the honeybee host.
Article
Biotechnology & Applied Microbiology
Meng Li, Jia-ning Shi, Gao-ming You, Yong-sheng Ma, Qian-cheng Zhao
Summary: This study evaluated the bacterial growth of chilled fish during storage after shipping and the interactions between selected bacterial strains using Coregonus peled fillets as a model. The results showed that the predominant microorganisms after 12 days of storage were Aeromonas, Pseudomonas, Carnobacterium, Psychrobacter, and Shewanella. Moreover, all test strains exhibited inhibiting effects on the growth of other strains. This study provides valuable information on the microbiological spoilage of C. peled and can be used for the manufacturing and preservation of C. peled products.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Marija Vrdoljak, Milna Tudor Kalit, Iva Dolencic Spehar, Biljana Radeljevic, Marko Jelic, Sandra Mandinic, Jadranka Frece, Samir Kalit
Summary: This study found that autochthonous probiotic bacteria Lactobacillus plantarum B and Lactococcus lactis subsp. lactis S1 have significant effects on proteolysis during the ripening of Sir iz misine cheese. The addition of Lactobacillus plantarum B alone or in combination with Lactococcus lactis subsp. lactis S1 increased the alpha and beta indices, improving the texture and ripening properties of the cheese.
FERMENTATION-BASEL
(2022)
Article
Biotechnology & Applied Microbiology
Chunguang Luan, Weihua Cao, Na Luo, Jingxia Tu, Jianqin Hao, Yihong Bao, Feike Hao, Deliang Wang, Xin Jiang
Summary: In this study, the complete genome sequence of a novel beer-spoilage bacterium Lactobacillus acetotolerans CN247 was reported. The strain exhibited tolerance to acetic acid, ethanol, and streptomycin, and showed varying degrees of preference for different beer components. Genes associated with viable but non-culturable (VBNC) bacteria were also identified in the genome.
JOURNAL OF THE AMERICAN SOCIETY OF BREWING CHEMISTS
(2023)
Article
Biotechnology & Applied Microbiology
Yayoi Gotoh, Kyosuke Kita, Kosei Tanaka, Shu Ishikawa, Toshio Suzuki, Ken-ichi Yoshida
Summary: Strains of Lactococcus lactis subsp. cremoris are commonly used for yogurt production with exopolysaccharides, but a mutant EPSC lacking this biosynthesis ability was isolated under elevated temperature conditions. Genomic analysis revealed the selective loss of a plasmid encoding exopolysaccharide biosynthesis cluster in EPSC, offering insights into the mechanism of exopolysaccharide synthesis in yogurt production.
JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY
(2021)
Article
Microbiology
Gabriela Christina Kuhl, Ricardo Ruiz Mazzon, Rubens Tadeu Delgado Duarte, Juliano De Dea Lindner
Summary: Several strains of Lactobacillus are known as potential producers of conjugated linoleic acid (CLA), with L. bulgaricus strain LBP UFSC 2230 being identified as a candidate for CLA production in fermented dairy products. The draft genome sequence of this strain, isolated from Italian Grana Padano cheese, provides valuable insights into its metabolic capabilities, particularly the identification of an enzyme involved in linoleic acid biohydrogenation, oleate hydratase.
BRAZILIAN JOURNAL OF MICROBIOLOGY
(2021)
Article
Parasitology
M. A. Nicdao, P. C. Ingalla, J. Ibana
Summary: Antimicrobial resistance is a global crisis. Probiotics, such as Lactobacillus species, can inhibit the growth of gut pathogens. Our study shows that Salmonella can enhance the antimicrobial activities of Lactobacillus species. These findings have potential applications in food processing and preservation strategies to control Salmonella contamination.
TROPICAL BIOMEDICINE
(2023)
Article
Biotechnology & Applied Microbiology
Ilhan Cem Duru, Anne Ylinen, Sergei Belanov, Alan Avila Pulido, Lars Paulin, Petri Auvinen
Summary: This study sheds new light on the cold- and heat-shock response of psychrotrophic LAB. Candidate genes identified through gene network inference analysis could serve as targets for future research.
Article
Clinical Neurology
Atte Sjovall, Velma T. E. Aho, Taneli Hyyrynen, Teemu J. Kinnari, Petri Auvinen, Juha Silvola, Antti Aarnisalo, Anu Laulajainen-Hongisto
Summary: The study investigated the microbiota of the healthy external auditory canal culture-independently and evaluated the swabbing method for EAC microbiota sample collection. It found that Staphylococcus auricularis, Propionibacterium acnes, Alloiococcus otitis, and Turicella otitidis were the most common bacteria in the EAC microbiome. The EAC microbiota appeared to be more diverse and individualized than previously thought, and ear cleaning habits could influence the EAC microbiome.
OTOLOGY & NEUROTOLOGY
(2021)
Article
Microbiology
Jacob Holmer, Velma Aho, Maria Eriksdotter, Lars Paulin, Milla Pietiainen, Petri Auvinen, Marianne Schultzberg, Pirkko J. Pussinen, Kare Buhlin
Summary: The study compared subgingival microbiota of individuals with Alzheimer's disease, mild cognitive impairment, subjective cognitive decline, and cognitively healthy controls. Results showed shifts in the subgingival microbiota typical of periodontal disease in individuals with cognitive impairment or AD.
JOURNAL OF ORAL MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Ilhan Cem Duru, Florentina Ionela Bucur, Margarita Andreevskaya, Bahareh Nikparvar, Anne Ylinen, Leontina Grigore-Gurgu, Tone Mari Rode, Peter Crauwels, Pia Laine, Lars Paulin, Trond Lovdal, Christian U. Riedel, Nadav Bar, Daniela Borda, Anca Ioana Nicolau, Petri Auvinen
Summary: The study found that L. monocytogenes activated a general stress response after high-pressure processing, with the phosphotransferase system, protein folding, and cobalamin biosynthesis being the most upregulated genes in the recovery process. Cell-division-related genes were downregulated while peptidoglycan-synthesis genes were upregulated, suggesting cell wall repair is involved in the recovery from HPP damage. Additionally, a non-coding RNA (Rli47) was predicted to play a role in HPP damage recovery, and gene-deletion experiments showed pbp2A mutants are more sensitive to HPP.
Article
Neurosciences
Velma T. E. Aho, Madelyn C. Houser, Pedro A. B. Pereira, Jianjun Chang, Knut Rudi, Lars Paulin, Vicki Hertzberg, Petri Auvinen, Malu G. Tansey, Filip Scheperjans
Summary: This study found that intestinal inflammatory responses and reductions in fecal SCFAs occur in Parkinson's disease (PD), are related to the microbiota and to disease onset, and are not reflected in plasma inflammatory profiles. Some of these relationships are distinct in PD and are sex-dependent. This study revealed potential alterations in microbiota-host interactions and links between earlier PD onset and intestinal inflammatory responses and reduced SCFA levels.
MOLECULAR NEURODEGENERATION
(2021)
Article
Multidisciplinary Sciences
Kean-Jin Lim, Tanja Paasela, Anni Harju, Martti Venalainen, Lars Paulin, Petri Auvinen, Katri Karkkainen, Teemu H. Teeri
Summary: The stress response of five-year-old Scots pine xylem to mechanical wounding was studied using RNA sequencing, revealing a bimodal response with early induction of transcripts related to stress, defense, and cell wall modification, followed by a later wave of secondary metabolism activation. Stilbene biosynthesis, particularly pinosylvin and its monomethyl ether, was induced after wounding, showing similarities and differences compared to heartwood formation responses. Pine resin biosynthesis was not induced in the initial four days post-wounding.
SCIENTIFIC REPORTS
(2021)
Article
Biotechnology & Applied Microbiology
Bahareh Nikparvar, Margarita Andreevskaya, Ilhan C. Duru, Florentina I. Bucur, Leontina Grigore-Gurgu, Daniela Borda, Anca I. Nicolau, Christian U. Riedel, Petri Auvinen, Nadav Bar
Summary: The pathogen Listeria (L.) monocytogenes exhibits different gene regulation modes in response to high pressure processing, including survival mode, repair mode, and re-growth mode. This allows for the timely activation of transcription factors associated with immediate stress response, followed by repair and re-growth to survive and recover from extreme conditions. The findings provide insights for potential design of specific strategies to target genes or mechanisms to prevent L. monocytogenes recovery during food storage under high pressure conditions.
Article
Virology
Pia Laine, Hanna Nihtila, Ella Mustanoja, Annina Lyyski, Anne Ylinen, Jukka Hurme, Lars Paulin, Sakari Jokiranta, Petri Auvinen, Taru Meri
Summary: While most spontaneous mutations in the viral genome have no consequences, some can affect diagnostic tests. A variant strain found in Southern Finland in February 2021 showed mutations in the N gene that led to negative results in PCR tests. This variant belongs to the B.1.1.318 Pango lineage and differs from the Beta variant in certain aspects.
JOURNAL OF MEDICAL VIROLOGY
(2022)
Article
Ecology
Artur Trzebny, Anna Slodkowicz-Kowalska, Johanna Bjorkroth, Miroslawa Dabert
Summary: This study investigated the impact of microsporidians on the composition of mosquito gut microbiota. The results showed that the composition of mosquito gut microbiota varies among host species and is correlated with the presence of microsporidians. Additionally, microsporidian infection is associated with specific metabolic functions and certain bacterial species.
Article
Biology
Olli-Pekka Smolander, Daniel Blande, Virpi Ahola, Pasi Rastas, Jaakko Tanskanen, Juhana I. Kammonen, Vicencio Oostra, Lorenzo Pellegrini, Suvi Ikonen, Tad Dallas, Michelle F. DiLeo, Anne Duplouy, Ilhan Cem Duru, Pauliina Halimaa, Aapo Kahilainen, Suyog S. Kuwar, Sirpa O. Kaerenlampi, Elvira Lafuente, Shiqi Luo, Jenny Makkonen, Abhilash Nair, Maria de la Paz Celorio-Mancera, Ville Pennanen, Annukka Ruokolainen, Tarja Sundell, Arja I. Tervahauta, Victoria Twort, Erik van Bergen, Janina Osterman-Udd, Lars Paulin, Mikko J. Frilander, Petri Auvinen, Marjo Saastamoinen
Summary: Researchers provide a chromosome-level assembly of the Glanville fritillary butterfly genome from Pacific Biosciences sequencing of a pool of males, which is a valuable resource for future comparative genomics, molecular biology, transcriptome, and genetics studies.
Article
Biotechnology & Applied Microbiology
Per Johansson, Elina Sade, Jenni Hultman, Petri Auvinen, Johanna Bjorkroth
Summary: Genomic analysis of 37 strains of Leuconostoc gelidum and Leuconostoc gasicomitatum supports the reclassification of L. gelidum subsp. gasicomitatum to L. gasicomitatum at the species level. The study also revealed limited intraspecies variation in genome properties, which were not attributed to the source of isolation. Additionally, none of the strains carried acquired antimicrobial resistance genes or genes associated with virulence, indicating the lack of functional and phylogenetic diversification driving their distribution and lifestyle in foods.
Article
Biotechnology & Applied Microbiology
Elina Jaaskelainen, Elina Sade, Tuukka Ronkko, Jenni Hultman, Per Johansson, Marja-Liisa Riekkola, Johanna Bjorkroth
Summary: Marination of fish products can increase psychrotrophic bacteria concentration and tyramine formation, while unmarinated products have higher levels of trimethylamine. Lactic acid bacteria dominate in marinated strips, while Fusobacteriaceae and lactic acid bacteria dominate in unmarinated strips.
Article
Biology
Ilhan Cem Duru, Florentina Ionela Bucur, Margarita Andreevskaya, Anne Ylinen, Peter Crauwels, Leontina Grigore-Gurgu, Bahareh Nikparvar, Tone Mari Rode, Pia Laine, Lars Paulin, Trond Lovdal, Christian U. Riedel, Nadav Bar, Daniela Borda, Anca Ioana Nicolau, Petri Auvinen
Summary: The study focused on generating the whole genome sequence of L. monocytogenes strain S2542 and comparing it to strains RO15 and ScottA, as well as comparing gene expression profiles after high-pressure processing. Results showed that S2542 belongs to serotype 4b and is more susceptible to treatment at 400 MPa, with viable cell counts below the limit of detection after HPP. Transcriptomic responses to HPP were not significantly different among the three strains.
BMC RESEARCH NOTES
(2021)