Article
Microbiology
Hasan Tukenmez, Pardeep Singh, Souvik Sarkar, Melike Cakir, Ana H. Oliveira, Cecilia Lindgren, Karolis Vaitkevicius, Mari Bonde, A. Elisabeth Sauer-Eriksson, Fredrik Almqvist, Jorgen Johansson
Summary: Bacteria resistant to antibiotics are a major problem, requiring the development of new antimicrobial drugs. In this study, the effectiveness of a new compound, PS900, in inhibiting virulence factor expression in Listeria monocytogenes was demonstrated. Furthermore, PS900 was found to potentiate the bactericidal effects of cholic acid and osmolytes, making it a promising candidate for future antibacterial drug design.
Article
Immunology
Josephine Moran, Liam Feltham, James Bagnall, Marie Goldrick, Elizabeth Lord, Catherine Nettleton, David G. Spiller, Ian Roberts, Pawel Paszek
Summary: The paper investigates the real-time interaction between the food-borne pathogen L. monocytogenes and host macrophages, revealing heterogeneous outcomes of infection and the impact of bacterial quantity on macrophage phagocytosis ability. It also highlights the role of population-level PrfA-mediated activity in regulating host-pathogen interactions.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Microbiology
Zhe Zeng, Siming Li, Sjef Boeren, Eddy J. Smid, Richard A. Notebaart, Tjakko Abee
Summary: Listeria monocytogenes can form bacterial microcompartments (BMCs) for substrate utilization, such as 1,2-propanediol, potentially linked to rhamnose metabolism. This study demonstrates that vitamin B-12-dependent activation of pdu stimulates anaerobic growth of L. monocytogenes on rhamnose via BMC-dependent 1,2-propanediol utilization, highlighting the physiological effects and energy efficiency of this pathway.
Article
Immunology
David R. Mains, Samuel J. Eallonardo, Nancy E. Freitag
Summary: The study shows that L. monocytogenes prfA* mutants exhibit significantly enhanced resistance to oxidative stress. Furthermore, transposon mutagenesis led to the identification of three novel gene targets required for full oxidative stress resistance only in the context of PrfA activation. Additionally, inactivation of cbpA and lmo0779 in a prfA* background resulted in reduced virulence in infected mice.
INFECTION AND IMMUNITY
(2021)
Article
Food Science & Technology
Carolina Ibarguren, M. Virginia Guitian, Romina M. Lenz, Soria M. Cecilia, M. Carina Audisio
Summary: Listeria monocytogenes is a foodborne pathogen that can survive in harsh food processing environments. The use of bacteriocins as a natural alternative to control this pathogen has been hindered by the emergence of resistant strains. This study compares the properties of susceptible and resistant strains, including growth, antibiotic susceptibility, cell morphology, virulence factor expression, and biofilm formation. The results contribute to the understanding of bacteriocin-resistance development in L. monocytogenes and its implications for the use of these peptides as biopreservatives.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Lisa Teubner, Renate Frantz, Luigi La Pietra, Martina Hudel, Jasmin Bazant, Gunter Lochnit, Lena Eismann, Guenter Kramer, Trinad Chakraborty, Mobarak Abu Mraheil
Summary: Protein secretion is a key process for Listeria monocytogenes to interact with its environment. Recent research has shown that RNA secretion is also an important strategy for the pathogen to manipulate host cell response. In this study, the involvement of SecA2 in ribosome sedimentation and its association with a subset of secreted sRNAs that induce high levels of IFN-beta response in host cells were demonstrated.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Food Science & Technology
Rene Koppel, Jasmin Schade, Martin Peier
Summary: Listeria in food poses a serious risk to consumer health, and reliable methods are needed for detection. While various PCR systems have been developed for identifying Listeria strains, they may not detect all known strains. This study introduces a novel multiplex real-time PCR method that can simultaneously detect multiple common and known Listeria strains.
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
(2021)
Article
Food Science & Technology
Svetoslav Dimitrov Todorov, Wilhelm Heinrich Holzapfel, Luis Augusto Nero
Summary: The Pediococcus acidilactici strains isolated from Brazilian artisanal cheese showed high bacteriocin activity against Listeria monocytogenes and Enterococcus spp. The bacteriocins produced by these strains exhibited bactericidal mode of action against certain strains and displayed safety-related genes, although the presence of possible virulence factors and antibiotic resistance should be further investigated.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Microbiology
Inna L. Garmasheva, Ljubov T. Oleschenko
Summary: This study aimed to select bacteriocinogenic strains among Enterococcus strains isolated from Ukrainian traditional dairy products. Molasses and steep corn liquor were used as a low-cost media for screening. The results showed that 34 Enterococcus strains produced metabolites that inhibited the growth of indicator strains, and five strains were found to possess enterocin genes.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Food Science & Technology
Cristina Merla, Marta Corbella, Gherard Batisti Biffignandi, Stefano Gaiarsa, Michele Castelli, Giuseppina Andreoli, Massimo Fabbi, Bianca Mariani, Davide Sassera, Piero Marone, Patrizia Cambieri
Summary: The study found that there were multiple strains of L. monocytogenes circulating in a small area of northern Italy, but no association was found between clinical isolates and contaminated food samples.
FOODBORNE PATHOGENS AND DISEASE
(2021)
Article
Food Science & Technology
Danielle Nader Furtado, Bernadette Dora Gombossy de Melo Franco, Svetoslav Dimitrov Todorov
Summary: Differentiated Lactococcus lactis bacteriocin producers' strains using repPCR technique. The stability of bacteriocin was found to be influenced by applied chemicals, pH, and temperature. The addition of bacteriocin completely inhibited the growth of Listeria monocytogenes ScottA, Lactobacillus sakei ATCC 15521, or Enterococcus faecium ATCC 19434 in the study.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Infectious Diseases
Pavel A. Andriyanov, Pavel A. Zhurilov, Elena A. Liskova, Tatyana I. Karpova, Elena V. Sokolova, Yulia K. Yushina, Elena V. Zaiko, Dagmara S. Bataeva, Olga L. Voronina, Ekaterina K. Psareva, Igor S. Tartakovsky, Denis V. Kolbasov, Svetlana A. Ermolaeva
Summary: Susceptibility testing of 117 L. monocytogenes strains to 23 antibiotics revealed a varied response, with most strains showing sensitivity to multiple antibiotics but some strains exhibiting resistance to specific drugs. There was a shift in the proportion of resistant strains over time, with different antibiotics showing unique patterns of resistance evolution.
Article
Microbiology
Zhe Zeng, Sjef Boeren, Varaang Bhandula, Samuel H. Light, Eddy J. Smid, Richard A. Notebaart, Tjakko Abee
Summary: The study reveals a BMC-dependent mechanism for ethanolamine utilization in Listeria monocytogenes, activated by vitamin B-12 and leading to growth. Additionally, BMC formation was found to be connected with ethanolamine catabolism, resulting in the production of acetate and ethanol in a 2:1 ratio.
Article
Food Science & Technology
Constanza Melian, Emilse Bentencourt, Patricia Castellano, Diego Ploper, Graciela Vignolo, Lucia M. Mendoza
Summary: This study investigates the use of anti-listerial bacteriocins to prevent/control biofilm formation of Listeria monocytogenes strains at low temperatures. The results show that subinhibitory concentrations of bacteriocins reduce biofilm formation and affect gene expression levels.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2022)
Article
Food Science & Technology
Xiaoli Huang, Yao He, Changcheng Zhong, Kui Zhao, Nagendra P. Shah, Xueying Tao, Hua Wei
Summary: In this study, Lactiplantibacillus plantarum strains were isolated from Hu sheep feces and L. plantarum ELPL14 was identified as a potential probiotic with the ability to inhibit Listeria monocytogenes growth in pasteurized milk. The presence of eleven plantaricin genes in ELPL14 suggested that the inhibition of L. monocytogenes may be due to bacteriocin production. These findings indicate that L. plantarum ELPL14 from Hu sheep has the potential to be used as a natural dairy additive for inhibiting food-borne pathogens, especially L. monocytogenes.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)