Article
Plant Sciences
Zhiyan Wang, Feng-Xian Yang, Chongxi Liu, Li Wang, Yuxin Qi, Minghang Cao, Xiaowei Guo, Jie Li, Xueshuang Huang, Jing Yang, Sheng-Xiong Huang
Summary: Nexphenazine A, a phenazine-polyketide hybrid compound, was isolated from Streptomyces sp. KIB-H483. The biosynthesis of nexphenazine involves a gene cluster, with npzI gene playing a key role.
JOURNAL OF NATURAL PRODUCTS
(2022)
Article
Plant Sciences
Huiming Huang, Liangguang Yue, Fayu Deng, Xiaoyu Wang, Ning Wang, Hu Chen, Huayue Li
Summary: The integration of NMR-metabolomic and genomic analyses allows for enhanced identification of structural properties and biosynthetic information in natural products. In this study, NMR-based metabolomic profiling and genome mining were employed to isolate and determine the structures of two new natural products from Streptomyces sp. S063. The cytotoxicity evaluation demonstrated moderate growth inhibition against several human cancer cells.
JOURNAL OF NATURAL PRODUCTS
(2023)
Article
Agriculture, Multidisciplinary
Yu Li, Zifei Xu, Ping Chen, Chen Zuo, Liyifan Chen, Wei Yan, Ruihua Jiao, Yonghao Ye
Summary: A type II polyketide synthase biosynthetic gene cluster (nap) was found in Streptomyces eurocidicus CGMCC 4.1086 through genome mining. Heterologous expression of the nap gene cluster in Streptomyces albus J1074 produced dimerized aromatic polyketides naphthocyclinones (1-3), which were structurally characterized by extensive analysis. The synthesis pathway of naphthocyclinones was revealed through genetic manipulation, biochemical reactions, and comparative genomics. Remarkably, compound 3 exhibited strong inhibitory effects against Phytophthora capsici and Phytophthora sojae, showing promising potential for plant disease control.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Plant Sciences
Thibault Caradec, Xavier Trivelli, Eva Desmecht, Veronique Peucelle, Jamal Khalife, Ruben C. Hartkoorn
Summary: A series of novel macrolides, named dactylosporolides A-C, were discovered from the culture supernatant of rare soil actinobacteria Dactylosporangium fulvum. The structure and absolute configuration of these macrolides were determined using NMR structural elucidation and analysis of the biosynthetic gene cluster. Although they do not possess antibiotic activity, these dactylosporolides displayed activity against Plasmodium falciparum 3D7.
JOURNAL OF NATURAL PRODUCTS
(2022)
Article
Biochemistry & Molecular Biology
Wei Zhou, Hattan A. Alharbi, Eshe Hummingbird, Adrian T. Keatinge-Clay, Taifo Mahmud
Summary: This study identified that NFAT-133 is not derived from TM-123, but from a programmed extension process of a nascent heptaketide in the PKS assembly line. Mutants of the ATCC 27456 strain also revealed the presence of NFAT-133/TM-123 analogues and the involvement of NftN, NftE, and NftG in the polyketide chain assembly processes.
ACS CHEMICAL BIOLOGY
(2022)
Article
Biology
Xuejin Zhao, Yeqing Zong, Weijia Wei, Chunbo Lou
Summary: This study presents a method for recombining the thaxtomin A gene cluster and improving its yield by obtaining high-yielding Streptomyces hosts. Additionally, an efficient gene editing method based on CRISPR/Cas9 digestion and yeast homologous recombination was developed. Results showed that by replacing promoters and optimizing gene cluster structure, the yield of thaxtomin A was significantly improved.
Article
Microbiology
Kohta Unno, Shinya Kodani
Summary: Recently, a new biosynthetic gene cluster of co-ester-containing peptides (OEPs) was discovered in the genome of actinobacterium Streptomyces prunicolor. A new OEP named prunipeptin was successfully produced through heterologous co-expression of precursor peptide and ATP-grasp ligase genes. Further mutagenesis experiment resulted in a variant peptide prunipeptin 15HW, and the bridging pattern of this peptide was determined through chemical cleavage and MS experiments, indicating the catalytic function of ATP-grasp ligase in forming intramolecular bonds.
MICROBIOLOGICAL RESEARCH
(2021)
Review
Biochemistry & Molecular Biology
Songya Zhang, Yunliang Chen, Jing Zhu, Qiujie Lu, Max J. Cryle, Youming Zhang, Fu Yan
Summary: Streptomyces bacteria are widely distributed in terrestrial and marine environments and are a rich source of active natural products due to their metabolic diversity. This review highlights the importance of nonribosomal lipopeptides as important natural products with diverse biological activities that play crucial roles in the lifestyle of Streptomyces. Recent advances in the biosynthesis of lipopeptide antibiotics produced by Streptomyces have greatly contributed to our understanding of their structures, properties, biosynthetic mechanisms, chemical and chemoenzymatic synthesis, and biological functions. The use of genome mining techniques has led to the discovery of many novel lipopeptides, further demonstrating their potential for future development in modern medicine.
NATURAL PRODUCT REPORTS
(2023)
Article
Biochemical Research Methods
Nadia Eusebio, Raquel Castelo-Branco, Diana Sousa, Marco Preto, Paul D'Agostino, Tobias A. M. Guider, Pedro N. Leao
Summary: A study discovered 12 new variants of microginins in a cyanobacterium, containing uncommon amino acids. Heterologous expression of the relevant biosynthetic gene cluster led to the production of several microginins, providing a pathway for accessing new variants through genome data or pathway engineering.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Chemistry, Organic
Jiafan Yang, Yongxiang Song, Man-Cheng Tang, Mingzhe Li, Junwei Deng, Nai-Kei Wong, Jianhua Ju
Summary: A genome-directed discovery strategy was used to identify 11 new tetrahydroisoquinolines (THIQs) from deep-sea derived Streptomyces, including aclidinomycins A and B as well as nine new compounds. The complex structures of these compounds, which contain unique polycyclic motifs, were elucidated through extensive spectroscopic analyses. Additionally, a proposed biosynthetic route and preliminary survey of their antimicrobial activities were reported.
JOURNAL OF ORGANIC CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Ximing Chen, Shuyan Li, Binglin Zhang, Haili Sun, Jinxiu Wang, Wei Zhang, Wenbo Meng, Tuo Chen, Paul Dyson, Guangxiu Liu
Summary: The discovery and functional characterization of a new bacterial tRNA species is reported. This tRNA decodes specific codons and circumvents inefficient base-pairing during translation, leading to increased antibiotic and bioactive metabolite production.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Chemistry, Organic
Zhi Kai Guo, Yong Chao Wang, Ying Zi Tan, Ailiman Abulaizi, Zi Jun Xiong, Shi Qing Zhang, Yang Yang, La Ying Yang, Jing Shi
Summary: Chemical investigation of Streptomyces sp. NA07423 led to the discovery of two unreported macrolactams, nagimycins A(1) and B (2). Their structures were elucidated by NMR, HRESIMS, X-ray crystallography, and comparison of experimental and theoretical ECD spectra. The nagimycins have a unique butenolide moiety rarely found in ansamycin antibiotics. Genome analysis revealed the putative biosynthetic gene cluster for nagimycins, and a likely biosynthetic pathway was proposed. Notably, compounds 1 and 2 exhibited potent antibacterial activity against two pathogenic Xanthomonas bacteria.
Article
Microbiology
Yunsheng Gao, Joy Birkelbach, Chengzhang Fu, Jennifer Herrmann, Herbert Irschik, Bernd Morgenstern, Kerstin Hirschfelder, Ruijuan Li, Youming Zhang, Rolf Jansen, Rolf Mueller
Summary: Microbial secondary metabolites are abundant sources of bioactive compounds and are privileged scaffolds for the development of new drugs. Therefore, continuous discovery of novel bioactive natural products is crucial for pharmaceutical research. Myxobacteria, particularly Sorangium spp., are known for their underexploited biosynthetic potential and serve as proficient producers of such secondary metabolites. In this study, a family of natural products called disorazole Z was isolated and characterized from Sorangium cellulosum strain So ce1875, showing potent anticancer activity. Furthermore, the biosynthesis and heterologous production of disorazole Z were reported, providing potential for the development of disorazole-based anticancer drugs for clinical studies.
MICROBIOLOGY SPECTRUM
(2023)
Article
Multidisciplinary Sciences
Shupeng Yin, Zhi Liu, Jingjing Shen, Yuwei Xia, Weihong Wang, Pengyan Gui, Qian Jia, Konthorn Kachanuban, Weiming Zhu, Peng Fu
Summary: The study reports the discovery of eight chimeric medermycin-type natural products and their nonenzymatic construction. These synthetic compounds show potent antibacterial activity against drug-resistant bacteria and pave the way for understanding complex natural product formation and synthesizing derivatives.
NATURE COMMUNICATIONS
(2022)
Article
Chemistry, Organic
Yang Liu, Haibo Zhou, Qiyao Shen, Guangzhi Dai, Fu Yan, Xiaoju Li, Xiangmei Ren, Qin Sun, Ya-Jie Tang, Youming Zhang, Xiaoying Bian
Summary: In this study, a cryptic trans-acyltransferase polyketide synthase biosynthetic gene cluster was cloned from Streptomyces sp. B59 and transferred into a heterologous host, resulting in the synthesis of a class of new compounds. Heterologous expression experiments identified two biosynthetic intermediates and suggested the involvement of an unusual oxidoreductase in the formation of a rare moiety in these compounds.
