Article
Agronomy
Chuangju Ma, Jinsong Xiong, Morong Liang, Xiaoyu Liu, Xiaodong Lai, Yibo Bai, Zongming Cheng
Summary: The study revealed that FvWRKY50 acts as a positive regulator in mediating resistance against B. cinerea by regulating some JA pathway and defense-related genes.
Article
Biochemistry & Molecular Biology
Tayb Elassma I. M. DafaAlla, Mohnad Abdalla, Amr Ahmed El-Arabey, Wafa Ali Eltayb, Ranjan K. Mohapatra
Summary: Botrytis cinerea, an economically important necrotrophic fungus, plays a critical role in infecting various plants. The study identified an up-regulation of the BcADH1 gene during tomato - B. cinerea interaction and demonstrated its importance in fungal development, environmental adaptation, and pathogenicity through knock-out mutant experiments. The Delta bcadh1 mutants showed morphological differences, increased sensitivity to hypoxia and reactive oxygen species, and lost their ability for alcoholic fermentation compared to the wild type and complementary strains.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2022)
Article
Microbiology
Mathias Choquer, Christine Rascle, Isabelle R. Goncalves, Amelie de Vallee, Cecile Ribot, Elise Loisel, Pavle Smilevski, Jordan Ferria, Mahamadi Savadogo, Eytham Souibgui, Marie-Josephe Gagey, Jean-William Dupuy, Jeffrey A. Rollins, Riccardo Marcato, Camille Nous, Christophe Bruel, Nathalie Poussereau
Summary: The study found that the necrotrophic plant-pathogen fungus Botrytis cinerea penetrates plant cell walls by secreting effectors, including plant toxins and proteases, while also playing a role in nutrition. Additionally, the research revealed significant remodeling of the infection cushion cell wall, suggesting a crucial role in colonization and nutrition of the pathogen.
ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Microbiology
Liang Ma, Tong Liu, Ke Zhang, Haojie Shi, Lei Zhang, Gen Zou, Amir Sharon
Summary: PCWDEs are considered important components of the virulence arsenal of necrotrophic plant pathogens. However, the role of PCWDEs in the pathogenicity of necrotrophic phytopathogenic fungi remains ambiguous. In this study, a transcription factor called BcXyr1 was identified in Botrytis cinerea that regulates the production of (hemi-)cellulases and is necessary for fungal virulence. Deletion of the bcxyr1 gene led to impaired spore germination, reduced fungal virulence, and decreased reactive oxygen species production. The findings highlight the importance of PCWDEs in the pathogenicity of B. cinerea and identify specific PCWDEs that play a crucial role.
Article
Plant Sciences
Ligang Chen, Liping Zhang, Shengyuan Xiang, Yanli Chen, Haiyan Zhang, Diqiu Yu
Summary: WRKY75 positively regulates plant defense responses against necrotrophic fungal pathogens by directly binding to the promoter of ORA59 and interacting with JASMONATE ZIM-domain proteins. This gene functions as a critical component of the JA-mediated signaling pathway in Arabidopsis.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Article
Biotechnology & Applied Microbiology
Danielle Duanis-Assaf, Ortal Galsurker, Olga Davydov, Dalia Maurer, Oleg Feygenberg, Moshe Sagi, Elena Poverenov, Robert Fluhr, Noam Alkan
Summary: Pathogenic fungi cause postharvest losses, prompting the need for new control strategies. The development of dsRNA-ERG effectively inhibits fungal growth and grey-mould development, reducing the required fungicide amount.
PLANT BIOTECHNOLOGY JOURNAL
(2022)
Article
Biotechnology & Applied Microbiology
Alicia Fernandez -San Millan, Luis Larraya, Jordi Gamir, Inmaculada Farran, Jon Veramendi
Summary: Research on yeasts as potential biocontrol agents against plant pathogens is gaining interest due to restrictions on the use of chemical pesticides. This study analyzed the exometabolome generated in a co-culture of the plant pathogen Botrytis cinerea with four antagonistic yeast strains, identifying antifungal mechanisms and molecules. The study found that the phenylpropanoid trans-cinnamic acid and the alkaloid indole-3carboxaldehyde were the best antagonistic metabolites against gray mold infection. This study provides insights into the roles of these metabolites in yeast metabolism and their potential applications in biotechnology.
BIOLOGICAL CONTROL
(2022)
Article
Food Science & Technology
Lifei Yang, Xiaoli Liu, Haiyan Lu, Cunzheng Zhang, Jian Chen, Zhiqi Shi
Summary: Cinnamaldehyde effectively controls Botrytis cinerea in fresh pepper fruit by inhibiting mycelial growth and spore germination, and inducing defense responses.
Article
Agronomy
Estefania Gonzalez-Fernandez, Roy Kennedy, Rachel Osborn, Maria Fernandez-Gonzalez, Francisco Javier Rodriguez-Rajo
Summary: The study developed immunological techniques for detecting grey mould disease, successfully measuring the fungal concentration in air and creating a predictive model that explains 40% variability in immune data.
Article
Multidisciplinary Sciences
Kei Hiruma, Seishiro Aoki, Junya Takino, Takeshi Higa, Yuniar Devi Utami, Akito Shiina, Masanori Okamoto, Masami Nakamura, Nanami Kawamura, Yoshihiro Ohmori, Ryohei Sugita, Keitaro Tanoi, Toyozo Sato, Hideaki Oikawa, Atsushi Minami, Wataru Iwasaki, Yusuke Saijo
Summary: In this study, a strain of the root fungus Colletotrichum tofieldiae (Ct), named Ct3, was found to severely inhibit plant growth by activating the host plant's abscisic acid pathways. This activation was related to a fungal secondary metabolism gene cluster involved in the biosynthesis of sesquiterpene metabolites. Activation of this cluster during root infection suppressed host nutrient uptake-related genes and altered mineral contents, manipulating the host plant's nutrition state. Disruption or environmental suppression of the cluster, however, made Ct3 beneficial for plant growth, depending on host plant's response regulators to phosphate starvation. These findings demonstrate that a fungal metabolism cluster can modulate the parasitic-mutualistic continuum of infectious fungi in fluctuating environments.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Yinmin Wang, Sida Zhang, Yong Xu, Haiyun Li, Ruihua Zhang, Dong Chen, Jianfu Xu, Xuemin Wu
Summary: Nanotechnology is revolutionizing modern agriculture by improving production efficiency and sustainability. Understanding the pesticide activity of nano- and conventional methods helps in developing new formulations. This study found that solid fluopyram formulations with nano-sized particles showed higher antifungal activity compared to larger particles.
Article
Biochemistry & Molecular Biology
Qianqian Yang, Jinguang Yang, Yameng Wang, Juan Du, Jianan Zhang, Ben F. Luisi, Wenxing Liang
Summary: Plant diseases have a significant impact on food security and are a global concern. The use of agrochemicals to control these diseases is currently common, but the development of drug resistance and off-target effects present challenges. This study identifies a mechanism of detoxification of reactive oxygen species (ROS) in fungi and develops potential fungicides to control plant diseases by targeting this mechanism.
Article
Horticulture
Mansoreh Hasanzadeh Naeemi, Sepideh Kalateh Jari, Vahid Zarinnia, Foad Fatehi
Summary: The study evaluated the effects of different concentrations of BR, MeJA, and fungal elicitor on rose flowers. The results showed that 1000 and 800 mg/l of fungal elicitor were the most effective treatments, improving the physiological traits and vase life of the flowers. Additionally, 0.1 and 0.2 mM MeJA and 1 μM BR significantly improved the quality of cut rose flowers.
SCIENTIA HORTICULTURAE
(2022)
Article
Agriculture, Multidisciplinary
Ghozlene Aouadi, Leila Kalai Grami, Faiza Taibi, Rym Bouhlal, Salem Elkahoui, Takoua Zaagueri, Slim Jallouli, Manel Chaanbi, Mohamed Rabeh Hajlaoui, Jouda Mediouni Ben Jemaa
Summary: This study investigated the chemical composition of Mentha pulegium and Myrtus communis essential oils and explored their antifungal activities against Botrytis cinerea. The results showed that Mentha pulegium essential oil exhibited better antifungal activity, inhibiting spore germination and inducing morphological changes in the fungus.
JOURNAL OF PLANT DISEASES AND PROTECTION
(2022)
Article
Chemistry, Applied
Ana Rita Zulim Leite, Flavia Aparecida Reitz Cardoso, Amanda Correia Gardenal, Joao Carlos Palazzo de Mello, Leila Larisa Medeiros Marques, Marcia Regina Ferreira Geraldo Perdoncini
Summary: The study evaluated the conservation and physicochemical characteristics of strawberries treated with crude plant extracts. The results showed that these extracts could effectively inhibit fungal growth and maintain the quality of strawberries. The changes in weight loss, soluble solids, acidity, and pH were analyzed, and it was found that different extracts had different effects on these characteristics.
NATURAL PRODUCT RESEARCH
(2023)
Article
Environmental Sciences
Anne Mette Madsen, John Kerr White, Amal Markouch, Sarah Kadhim, Nadieh de Jonge, Trine Thilsing, Vinni M. Hansen, Jesper Baelum, Jeppe Lund Nielsen, Ulla Vogel, Kira Tendal
Summary: Workers in greenhouses are exposed to a high concentration of fungi and bacteria, and this exposure level and microbial composition have an effect on their serum levels of CRP and SAA. The exposure levels and microbial composition are significantly associated with grouped work tasks and seasons.
ENVIRONMENTAL RESEARCH
(2021)
Article
Microbiology
Yonghui Zeng, Nupur, Naicheng Wu, Anne Mette Madsen, Xihan Chen, Alastair T. Gardiner, Michal Koblizek
Summary: A new member of chlorophototrophic Gemmatimonadetes bacteria, Gemmatimonas groenlandica, was isolated from a stream in High Arctic Greenland, showing unique features compared to previously known strains of CGB. The complete genome sequence of G. groenlandica reveals both distinctive and conserved characteristics in the photosynthesis gene clusters of CGB. Metagenome analysis of CGB from soil and glacier samples in Northeast Greenland suggests a wider distribution pattern of CGB beyond aquatic environments.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Engineering, Environmental
Anne Mette Madsen, Pil Uthaug Rasmussen, Margit W. Frederiksen
Summary: This study investigates the use of electrostatic dust cloths, called E-Cloths, to study the accumulation of microorganisms and endotoxin on workers' clothes. The results show that E-Cloths can effectively measure the accumulation of bacteria and fungi, primarily from the work environment and not the workers' skin.
Article
Environmental Sciences
Signe Agnete Moller, Pil Uthaug Rasmussen, Margit W. Frederiksen, Anne Mette Madsen
Summary: Work clothes can accumulate a large amount of microorganisms, especially fungi, which can be transported to the air via workers' clothes. Pathogenic and allergenic microorganisms were found on workers' clothes. Therefore, maintaining good clothing hygiene during and after work is crucial.
ENVIRONMENT INTERNATIONAL
(2022)
Article
Environmental Sciences
Anne Mette Madsen, John Kerr White, Jeppe Lund Nielsen, Mehmet Emin Keskin, Kira Tendal, Margit Wagtberg Frederiksen
Summary: Breeding pigeons is associated with respiratory symptoms, as exposure to bacteria and endotoxin in pigeon coops may contribute to airway issues among breeders. The airborne dust in pigeon coops contained a diverse range of bacterial species, some of which could pose potential health risks.
ENVIRONMENTAL RESEARCH
(2022)
Article
Archaeology
Camilla Jul Bastholm, Anne Mette Madsen, Birgitte Andersen, Jens Christian Frisvad, Jane Richter
Summary: Within the last decade, fungal colonisations have increased in Danish museum repositories. This study characterised a fungal colonisation in a museum repository and found that low humidity fungi were the main issue, questioning the effectiveness of the environmental guidelines in preventing fungal growth.
JOURNAL OF CULTURAL HERITAGE
(2022)
Article
Chemistry, Applied
Ana Sancho, Tina Birk, Juliane M. Gregersen, Tage Ronne, Sofie E. Hornslet, Anne M. Madsen, Katrine L. Bogh
Summary: This study investigated the variability of microbiota and protein content in birch sap throughout a complete tapping season, as well as the effect of freezing on its shelf life. The results showed that birch sap collected in the beginning of the season had a longer shelf life and contained less protein.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2022)
Review
Public, Environmental & Occupational Health
Lars Andrup, Karen A. Krogfelt, Kristian Schultz Hansen, Anne Mette Madsen
Summary: This study systematically reviewed the literature on the transmission routes of human rhinoviruses. The results showed that indirect transmission through hands and fomites is the dominant route of transmission in indoor settings, while airborne transmission is also a major route of transmission.
AMERICAN JOURNAL OF INFECTION CONTROL
(2023)
Article
Environmental Sciences
Anne Mette Madsen, Fei Zhang, Yonghui Zeng, Margit W. Frederiksen
Summary: This study investigated the potential human exposure to airborne microorganisms, including MRSA, in a pigeon exhibition in Denmark. The results showed high concentrations of fungi, bacteria, and endotoxin in the air, with concentrations up to 200 times higher than outdoor references. The bacteria found in the exhibition were mainly species found in pigeon coops, indicating that pigeons are the sources of exposure. The presence of airborne MRSA highlights the importance of considering this environment as a potential place of exchange of resistant bacteria between animals and humans.
ENVIRONMENTAL RESEARCH
(2023)
Article
Environmental Sciences
Elke Eriksen, Anani Komlavi Afanou, Anne Mette Madsen, Anne Straumfors, Pal Graff
Summary: Occupational exposure during waste sorting is associated with various health outcomes. This study compared the impact of work in fully automated waste sorting plants (AWSP) versus manual waste sorting plants (MWSP) on personal exposure to bioaerosols and related health effects. The results showed that workers at AWSP had lower exposure levels to inhalable dust, thoracic dust, and endotoxins compared to MWSP, but higher levels of fungal fragments, fungal spores, and actinobacterial spores. Microbial DNA exposure varied within and between types of waste sorting plants. Exposed workers also reported more common health symptoms compared to the unexposed control group.
ENVIRONMENTAL RESEARCH
(2023)
Article
Engineering, Environmental
Anne Mette Madsen, Katrine Uhrbrand, Victor Carp Kofoed, Thea K. Fischer, Margit W. Frederiksen
Summary: This study aimed to investigate whether serum levels of C-reactive protein (CRP) and serum amyloid A (SAA) were associated with personal exposure to endotoxin in wastewater treatment plant workers. The results showed significant correlations between CRP and both endotoxinLimulus and endotoxinrFC. SAA was also significantly associated with endotoxinLimulus. Therefore, it can be concluded that airborne endotoxin exposure has a negative impact on the health of wastewater treatment plant workers.
Article
Public, Environmental & Occupational Health
Karoline Kaergaard Hansen, Vivi Schlunssen, Karin Broberg, Kirsten Ostergaard, Margit W. Frederiksen, Anne Mette Madsen, Henrik Albert Kolstad
Summary: This study aimed to quantify current exposure levels of inhalable dust, endotoxin, and microorganisms among recycling workers. The results showed that production workers had significantly higher levels of exposure compared to administrative workers. Workers handling paper or cardboard had the highest exposure levels. Temperature did not affect exposure levels, but there was a tendency towards increased exposure to bacteria and fungi with higher temperatures.
ANNALS OF WORK EXPOSURES AND HEALTH
(2023)
Article
Public, Environmental & Occupational Health
Pil Uthaug Rasmussen, Katrine Uhrbrand, Margit W. Frederiksen, Anne Mette Madsen
Summary: In this study, we investigated the microbial exposure in nursing homes in Denmark. The results showed that personal exposure to microbes varied depending on the profession, with higher concentrations found in staff members involved in care and nursing tasks. We also found some multi-drug resistant isolates, indicating a potential risk for antimicrobial resistance transmission in these settings.
ANNALS OF WORK EXPOSURES AND HEALTH
(2023)
Article
Public, Environmental & Occupational Health
Elke Eriksen, Anne Mette Madsen, Anani Komlavi Afanou, Anne Straumfors, Alexander Eiler, Pal Graff
Summary: This study assessed microorganisms in personal inhalable work air samples from waste workers and found that bacterial and fungal concentrations varied significantly between waste sorting plants. A portion of the identified species were classified as human pathogens, and there were correlations between concentrations of certain fungi and self-reported adverse symptoms in exposed workers. The combination of culture-based methods and next generation sequencing contributed to the risk assessment of occupational exposure in waste sorting.
INTERNATIONAL JOURNAL OF HYGIENE AND ENVIRONMENTAL HEALTH
(2023)
Article
Public, Environmental & Occupational Health
Hanne Line Daae, Pal Graff, Oda Astrid Haarr Foss, Victor Carp Kofoed, Anani Komlavi Afanou, Margit Wagtberg Frederiksen, Anne Mette Madsen
Summary: This cross-sectional study investigated the exposure of workers in drilling waste treatment plants to harmful substances such as airborne dust, bacteria, and fungi. The results showed that some workers were exposed to high levels of endotoxin, bacteria, and fungi, with exposure levels depending on the treatment technique. The drilling waste contained large concentrations of bacteria, and elevated concentrations of airborne bacteria were found near drilling waste basins. Additionally, several pathogenic bacterial and fungal species were detected. Therefore, drilling waste treatment plants pose potential risks to the health of workers.
ANNALS OF WORK EXPOSURES AND HEALTH
(2023)