Article
Environmental Sciences
Natalija Topic Popovic, Snjezana Kazazic, Branka Bilic, Sanja Babic, Krunoslav Bojanic, Maro Bujak, Ivana Tartaro Bujak, Margita Jadan, Ivancica Strunjak-Perovic, Slavko Kepec, Rozelindra Coz-Rakovac
Summary: This study characterized Shewanella isolates from waters affected by a wastewater treatment plant and related fish, finding that they have the ability to produce fatty acids, high sensitivity to antimicrobial drugs, and low biofilm production capability. Exposure of zebrafish embryos to S. baltica resulted in significant developmental effects.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Boopathi Balasubramaniam, Thondimuthu Vinitha, Solai Meenal, Lappasi Mohanram VenkataKrishna, Velayutham Ravichandiran, Krishnaswamy Balamurugan
Summary: In eukaryotic organisms, cell signalling relies on post-translational modifications, and phosphorylation is a common and important modification. In this study, the importance of protein phosphorylation was investigated in C. elegans during bacterial infection. Proteomic techniques were used to identify differentially regulated proteins, and pathway analysis suggested the involvement of the MAPK pathway in innate immunity. The p38-MAPK protein, a crucial downstream component of the MAPK pathway, was characterized using various techniques. The study also revealed the involvement of proteolytic pathways in response to bacterial infection. Overall, the findings suggest that bacterial infection triggers the MAPK cascade to activate innate immunity and recruit proteolytic pathways.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Chemistry, Analytical
Frederic Jeanroy, Clothilde Comby-Zerbino, Claire Demesmay, Vincent Dugas
Summary: This study investigates the use of a new miniaturized affinity column to capture and elute high-affinity sequences from low molecular weight heparin for further characterization. Direct on-line analysis using MALDI-TOF MS was performed by applying the elution volume from the miniaturized affinity column directly to the MALDI plate.
ANALYTICA CHIMICA ACTA
(2023)
Review
Biochemistry & Molecular Biology
Charlotte A. Huber, Sarah J. Reed, David L. Paterson
Summary: Despite the diverse methods used in MALDI-TOF MS typing of bacterial strains, several research groups have shared common approaches. Combining multiple spectra of the same isolate before further analysis for strain distinction is a common practice. Many groups use a protein extraction step to enhance resolution in their MALDI-TOF MS results, often excluding peaks at a high mass range for data interpretation.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2021)
Article
Microbiology
Helena Vesela, Katerina Dorotikova, Marta Duskova, Petra Furmancikova, Ondrej Sedo, Josef Kamenik
Summary: This study analyzed the influence of fresh meat contamination on bacterial populations in raw materials and cooked hams. Heat treatment destroyed most bacteria, but some lactic acid bacteria remained.
Article
Microbiology
Charles Dumolin, Charlotte Peeters, Evelien De Canck, Nico Boon, Peter Vandamme
Summary: Culturomics-based bacterial diversity studies benefit from the implementation of MALDI-TOF MS to remove genomically redundant isolates. Network analysis based on unique spectral features provides a superior selection of genomically diverse OIUs compared to hierarchical clustering analysis and offers a better understanding of inter-OIU relationships.
Article
Environmental Sciences
Samet Kalkan
Summary: This study focused on the marine sediments in the Black Sea and explored the value of bacterial diversity for public health and biotechnology applications. Through the collection of sediment samples and the use of multimodal analytical platforms, 26 different bacterial species were identified, including some newly reported species. These findings contribute to a better understanding of microbial life in the southeastern sediments of the Black Sea.
MARINE POLLUTION BULLETIN
(2022)
Review
Microbiology
Fernando Sanchez-Juanes, Noelia Calvo Sanchez, Moncef Belhassen Garcia, Carmen Vieira Lista, Raul Manzano Roman, Rufino Alamo Sanz, Antonio Muro Alvarez, Juan Luis Munoz Bellido
Summary: Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a simple, fast, and specific method used for bacterial identification, and has also been shown to be useful for identifying certain parasites and arthropod vectors. Fast, simple, and reliable methods for identifying arthropod vectors are crucial for controlling diseases transmitted by these vectors.
Article
Plant Sciences
H. Nadeem, P. Niazi, M. Asif, G. Kaskavalci, F. Ahmad
Summary: The research revealed that lipopeptide molecules secreted by bacteria have antibiotic effects and demonstrate significant mortality rates on nematode eggs and juveniles. Root-dip treatment of tomato seedlings with lipopeptides can enhance plant growth attributes while reducing pathogen levels and root galling.
Article
Agriculture, Dairy & Animal Science
Leszek Guz, Aneta Nowakiewicz, Krzysztof Puk, Przemyslaw Zieba, Sebastian Gnat, Lukasz Matuszewski
Summary: The study identified a high prevalence of Aeromonas species in water sources and European pond turtles from Eastern Poland, with most isolates showing resistance to multiple antibiotics and carrying multiple virulence genes.
Review
Chemistry, Analytical
Hugo M. Santos, Luis B. Carvalho, Carlos Lodeiro, Goncalo Martins, Ines L. Gomes, Wilson D. T. Antunes, Vanessa Correia, Maria M. Almeida-Santos, Helena Rebelo-de-Andrade, Antonio P. A. Matos, J. L. Capelo
Summary: This work presents a tutorial on the capabilities of bioanalytical mass spectrometry in detecting and differentiating viruses at the peptide level, as well as providing diagnosis and prognosis for infected patients. It offers insights into the possibilities of current high-resolution mass spectrometry technology and bioinformatics.
MICROCHEMICAL JOURNAL
(2023)
Article
Microbiology
Mari Tohya, Kanae Teramoto, Shin Watanabe, Tomomi Hishinuma, Masahito Shimojima, Miho Ogawa, Tatsuya Tada, Yoko Tabe, Teruo Kirikae
Summary: The study compared different methods to identify Pseudomonas species and found that many of the clinical isolates, initially identified as P. putida or P. fluorescens, were misidentified. Whole-genome sequencing (WGS) and MALDI-TOF MS analysis revealed that these isolates actually belonged to different Pseudomonas species, including 9 novel species. The study suggests that WGS is the optimal method for Pseudomonas species identification, and MALDI-TOF MS has the potential to complement WGS.
MICROBIOLOGY SPECTRUM
(2022)
Article
Fisheries
Gaixiao Qin, Jin Xu, Xiaohui Ai, Yibin Yang
Summary: This study isolated a pathogenic strain ZZ051 from hemorrhagic channel catfish and identified it as Aeromonas veronii using physiological and biochemical methods, 16S rRNA gene sequencing, and MALDI-TOF-MS analysis. The isolated strain was confirmed to be the causative agent of the catfish disease through artificial infection. It was highly sensitive to enrofloxacin but resistant to florfenicol. This research provides a theoretical basis for preventing and controlling hemorrhagic disease in channel catfish.
Article
Microbiology
Eloise J. Busby, Ronan M. M. Doyle, Clara Leboreiro Babe, Kathryn A. A. Harris, Damien Mack, Gema Mendez-Cervantes, Denise M. M. O'Sullivan, Vicky Pang, Zahra Sadouki, Priya Solanki, Jim F. F. Huggett, Timothy D. D. McHugh, Emmanuel Q. Q. Wey
Summary: Managing the transmission of antimicrobial resistance requires reliable methods for tracking outbreaks. MALDI-TOF MS was compared with orthogonal approaches for strain typing, including WGS and FTIR, for Acinetobacter baumannii isolates associated with a healthcare-associated infection event. Reliable molecular typing methods can help to trace transmission routes and manage outbreaks.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Liangqiang Chen, Wenjing Gao, Xue Tan, Ying Han, Fu Jiao, Bin Feng, Jinghang Xie, Bin Li, Huilin Zhao, Huabin Tu, Shaoning Yu, Li Wang
Summary: MALDI-TOF MS is a method that can be used to classify specific model microbiota with high accuracy. The actual MS spectrum of the model microbiota shows a specific spectral fingerprint, which enhances the specificity and accuracy of microbiota classification.
MICROBIOLOGY SPECTRUM
(2023)
Article
Engineering, Environmental
Anne Mette Madsen, Pil Uthaug Rasmussen, Margit W. Frederiksen
Summary: This study investigates the use of electrostatic dust cloths, called E-Cloths, to study the accumulation of microorganisms and endotoxin on workers' clothes. The results show that E-Cloths can effectively measure the accumulation of bacteria and fungi, primarily from the work environment and not the workers' skin.
Article
Multidisciplinary Sciences
Pu Qian, Alastair T. Gardiner, Ivana Simova, Katerina Naydenova, Tristan Croll, Philip J. Jackson, Nupur, Miroslav Kloz, Petra Cubakova, Marek Kuzma, Yonghui Zeng, Pablo Castro-Hartmann, Bart van Knippenberg, Kenneth N. Goldie, David Kaftan, Pavel Hrouzek, Jan Hajek, Jon Agirre, C. Alistair Siebert, David Bina, Kasim Sader, Henning Stahlberg, Roman Sobotka, Christopher J. Russo, Tomas Polivka, C. Neil Hunter, Michal Koblizek
Summary: Phototrophic Gemmatimonadetes have evolved the ability to use solar energy through horizontal gene transfer. The study reveals the unique solar energy harvesting and trapping architecture of Gemmatimonas phototrophica, as well as the flow of energy within the complex.
Article
Environmental Sciences
Signe Agnete Moller, Pil Uthaug Rasmussen, Margit W. Frederiksen, Anne Mette Madsen
Summary: Work clothes can accumulate a large amount of microorganisms, especially fungi, which can be transported to the air via workers' clothes. Pathogenic and allergenic microorganisms were found on workers' clothes. Therefore, maintaining good clothing hygiene during and after work is crucial.
ENVIRONMENT INTERNATIONAL
(2022)
Article
Environmental Sciences
Anne Mette Madsen, John Kerr White, Jeppe Lund Nielsen, Mehmet Emin Keskin, Kira Tendal, Margit Wagtberg Frederiksen
Summary: Breeding pigeons is associated with respiratory symptoms, as exposure to bacteria and endotoxin in pigeon coops may contribute to airway issues among breeders. The airborne dust in pigeon coops contained a diverse range of bacterial species, some of which could pose potential health risks.
ENVIRONMENTAL RESEARCH
(2022)
Review
Public, Environmental & Occupational Health
Lars Andrup, Karen A. Krogfelt, Kristian Schultz Hansen, Anne Mette Madsen
Summary: This study systematically reviewed the literature on the transmission routes of human rhinoviruses. The results showed that indirect transmission through hands and fomites is the dominant route of transmission in indoor settings, while airborne transmission is also a major route of transmission.
AMERICAN JOURNAL OF INFECTION CONTROL
(2023)
Article
Environmental Sciences
Anne Mette Madsen, Fei Zhang, Yonghui Zeng, Margit W. Frederiksen
Summary: This study investigated the potential human exposure to airborne microorganisms, including MRSA, in a pigeon exhibition in Denmark. The results showed high concentrations of fungi, bacteria, and endotoxin in the air, with concentrations up to 200 times higher than outdoor references. The bacteria found in the exhibition were mainly species found in pigeon coops, indicating that pigeons are the sources of exposure. The presence of airborne MRSA highlights the importance of considering this environment as a potential place of exchange of resistant bacteria between animals and humans.
ENVIRONMENTAL RESEARCH
(2023)
Review
Engineering, Environmental
Tahseena Naaz, Ankit Kumar, Anusha Vempaty, Nupur Singhal, Soumya Pandit, Pankaj Gautam, Sokhee P. Jung
Summary: In the past two decades, there has been increasing interest in converting organic waste materials into bioenergy using microbial fuel cells (MFC) that degrade organic wastewater and generate electricity. However, the development of MFC has been limited by several constraints, including the development of the anode biofilm. This review examines the role of various biological components in the development of electroactive biofilm and discusses recent advances in genetic manipulations, electrode material modifications, electron exchange mechanisms, and operational strategies to improve anode performance of MFC. It also highlights the challenges and future directions for improving biofilm production.
ENVIRONMENTAL ENGINEERING RESEARCH
(2023)
Article
Microbiology
Sahana Shivaramu, Jurgen Tomasch, Karel Kopejtka, Nupur, Mohit Kumar Saini, Syed Nadeem Hussain Bokhari, Hendrik Kupper, Michal Koblizek
Summary: The bacterium Gemmatimonas phototrophica AP64, isolated from a freshwater lake in the western Gobi Desert, is the first phototrophic member of the bacterial phylum Gemmatimonadota. This strain was originally cultured on agar plates because it did not grow in liquid medium. However, the growth of G. phototrophica in liquid medium can be induced by supplementing the medium with 20 mg CaCl2 L-1.
Article
Biochemistry & Molecular Biology
Alastair T. Gardiner, Izabela Mujakic, David Bina, Zdenko Gardian, Karel Kopejtka, Nupur, Pu Qian, Michal Kobliz
Summary: Marine photoheterotrophic Gammaproteobacteria belonging to the NOR5/OM60 clade are the most abundant group of anoxygenic phototrophs. Little is known about their photosynthetic apparatus, so the photosynthetic complexes from the marine phototrophic bacterium Congregibacter litoralis KT71 were purified and characterized. These complexes contain bacteriochlorophyll a, spirilloxanthin, and two novel ketocarotenoids, with properties similar to complexes from purple bacteria. The organization of the RC-LH1 complex was further characterized using cryo-electron microscopy, and similarities to complexes from Thermochromatium tepidum were found.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2023)
Article
Environmental Sciences
Elke Eriksen, Anani Komlavi Afanou, Anne Mette Madsen, Anne Straumfors, Pal Graff
Summary: Occupational exposure during waste sorting is associated with various health outcomes. This study compared the impact of work in fully automated waste sorting plants (AWSP) versus manual waste sorting plants (MWSP) on personal exposure to bioaerosols and related health effects. The results showed that workers at AWSP had lower exposure levels to inhalable dust, thoracic dust, and endotoxins compared to MWSP, but higher levels of fungal fragments, fungal spores, and actinobacterial spores. Microbial DNA exposure varied within and between types of waste sorting plants. Exposed workers also reported more common health symptoms compared to the unexposed control group.
ENVIRONMENTAL RESEARCH
(2023)
Article
Engineering, Environmental
Anne Mette Madsen, Katrine Uhrbrand, Victor Carp Kofoed, Thea K. Fischer, Margit W. Frederiksen
Summary: This study aimed to investigate whether serum levels of C-reactive protein (CRP) and serum amyloid A (SAA) were associated with personal exposure to endotoxin in wastewater treatment plant workers. The results showed significant correlations between CRP and both endotoxinLimulus and endotoxinrFC. SAA was also significantly associated with endotoxinLimulus. Therefore, it can be concluded that airborne endotoxin exposure has a negative impact on the health of wastewater treatment plant workers.
Article
Public, Environmental & Occupational Health
Karoline Kaergaard Hansen, Vivi Schlunssen, Karin Broberg, Kirsten Ostergaard, Margit W. Frederiksen, Anne Mette Madsen, Henrik Albert Kolstad
Summary: This study aimed to quantify current exposure levels of inhalable dust, endotoxin, and microorganisms among recycling workers. The results showed that production workers had significantly higher levels of exposure compared to administrative workers. Workers handling paper or cardboard had the highest exposure levels. Temperature did not affect exposure levels, but there was a tendency towards increased exposure to bacteria and fungi with higher temperatures.
ANNALS OF WORK EXPOSURES AND HEALTH
(2023)
Article
Public, Environmental & Occupational Health
Pil Uthaug Rasmussen, Katrine Uhrbrand, Margit W. Frederiksen, Anne Mette Madsen
Summary: In this study, we investigated the microbial exposure in nursing homes in Denmark. The results showed that personal exposure to microbes varied depending on the profession, with higher concentrations found in staff members involved in care and nursing tasks. We also found some multi-drug resistant isolates, indicating a potential risk for antimicrobial resistance transmission in these settings.
ANNALS OF WORK EXPOSURES AND HEALTH
(2023)
Article
Public, Environmental & Occupational Health
Elke Eriksen, Anne Mette Madsen, Anani Komlavi Afanou, Anne Straumfors, Alexander Eiler, Pal Graff
Summary: This study assessed microorganisms in personal inhalable work air samples from waste workers and found that bacterial and fungal concentrations varied significantly between waste sorting plants. A portion of the identified species were classified as human pathogens, and there were correlations between concentrations of certain fungi and self-reported adverse symptoms in exposed workers. The combination of culture-based methods and next generation sequencing contributed to the risk assessment of occupational exposure in waste sorting.
INTERNATIONAL JOURNAL OF HYGIENE AND ENVIRONMENTAL HEALTH
(2023)
Article
Public, Environmental & Occupational Health
Hanne Line Daae, Pal Graff, Oda Astrid Haarr Foss, Victor Carp Kofoed, Anani Komlavi Afanou, Margit Wagtberg Frederiksen, Anne Mette Madsen
Summary: This cross-sectional study investigated the exposure of workers in drilling waste treatment plants to harmful substances such as airborne dust, bacteria, and fungi. The results showed that some workers were exposed to high levels of endotoxin, bacteria, and fungi, with exposure levels depending on the treatment technique. The drilling waste contained large concentrations of bacteria, and elevated concentrations of airborne bacteria were found near drilling waste basins. Additionally, several pathogenic bacterial and fungal species were detected. Therefore, drilling waste treatment plants pose potential risks to the health of workers.
ANNALS OF WORK EXPOSURES AND HEALTH
(2023)
