Article
Microbiology
Jeong In Hur, Jinshil Kim, Sangryeol Ryu, Byeonghwa Jeon
Summary: This study demonstrates the existence of phylogenetic clusters in Campylobacter jejuni associated with cold stress tolerance and identifies the genetic element cfrA as a contributor to cold stress tolerance.
MICROBIOLOGY SPECTRUM
(2022)
Article
Infectious Diseases
Nehal A. Saif, Jose F. Cobo-Diaz, Menattallah Elserafy, Iman El-Shiekh, Avelino Alvarez-Ordonez, Shaimaa F. Mouftah, Mohamed Elhadidy
Summary: This study characterized Campylobacter jejuni from different sources in Egypt using multiple typing techniques. The analysis revealed that cgMLST and host-segregating markers were the most discriminative methods, and identified retail chicken and cattle reservoir as important sources of C. jejuni infection in Egypt. The findings emphasize the importance of using whole genome sequencing typing methods for source tracking of C. jejuni and suggest the role of non-poultry sources in human campylobacteriosis control measures in Egypt.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Infectious Diseases
Rozan O. Al-Khresieh, Hala Al-Daghistani, Saeid M. Abu-Romman, Lubna F. Abu-Niaaj
Summary: This study investigates the link between human campylobacteriosis and the consumption of potentially contaminated food, finding that chicken meat is the main source of infection. The colonization rate of Campylobacter jejuni in chickens is influenced by the slaughterhouse environment, and antibiotic resistance is widespread among Campylobacter strains.
Article
Microbiology
Stephen Li, Jenna Lam, Leonidas Souliotis, Mohammad Tauqeer Alam, Chrystala Constantinidou
Summary: This study investigates the posttranscriptional regulation of Campylobacter jejuni (C. jejuni) by small noncoding RNAs (sRNAs) under different stress conditions. The researchers generated transcriptomic data from 21 host- and transmission-relevant conditions, which helped predict putative sRNAs and their biological functions. The results revealed a novel sRNA (CjSA21) that inhibits chemotaxis and promotes survival under food processing conditions. This research provides valuable insights into the adaptation of C. jejuni to stress conditions and highlights a potential chemotaxis inhibitor.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Dina Ramic, Blaz Jug, Katarina Simunovic, Magda Tusek Znidaric, Urban Kunej, Natasa Toplak, Minka Kovac, Marjorie Fournier, Polona Jamnik, Sonja Smole Mozina, Anja Klancnik
Summary: Campylobacter jejuni is a leading foodborne bacterial pathogen in humans. This study investigates the key role of the luxS gene in the biological processes of C. jejuni and reveals its importance in metabolic pathways, stress response, and survival.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Andrea Laconi, Roberta Tolosi, Ilenia Drigo, Luca Bano, Alessandra Piccirillo
Summary: This study assessed the ability of C. jejuni and C. coli strains isolated from diseased poultry to form biofilm. The results showed that C. jejuni strains were classified as no, weak, and moderate biofilm producers, while all C. coli strains were weak producers. Genes involved in chemotaxis, adhesion, and invasion were present in all C. jejuni strains. Further investigations are needed to better understand the genetic determinants involved in extra-intestinal Campylobacter biofilm formation.
VETERINARY MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Medelin Ocejo, Beatriz Oporto, Jose Luis Lavin, Ana Hurtado
Summary: Campylobacter, a leading cause of gastroenteritis in humans, asymptomatically colonises the intestinal tract of a wide range of animals. In this study, Illumina whole-genome sequencing was used to characterise AMR mechanisms in Campylobacter jejuni and Campylobacter coli from cattle and sheep in northern Spain. The analysis revealed extensive genetic diversity, identified resistance genotypes, and highlighted the importance of updated resistance gene databases for accurate AMR detection.
SCIENTIFIC REPORTS
(2021)
Article
Biology
Stephanie Peters, Ben Pascoe, Zuowei Wu, Sion C. Bayliss, Ximin Zeng, Adam Edwinson, Sakteesh Veerabadhran-Gurunathan, Selina Jawahir, Jessica K. Calland, Evangelos Mourkas, Robin Patel, Terra Wiens, Marijke Decuir, David Boxrud, Kirk Smith, Craig T. Parker, Gianrico Farrugia, Qijing Zhang, Samuel K. Sheppard, Madhusudan Grover
Summary: Researchers used whole-genome sequencing and phenotypic analysis to identify potential genetic factors linked to irritable bowel syndrome caused by Campylobacter jejuni infection. They found that specific genetic variations in bacterial stress response, adhesion protein, and core biosynthetic pathway genes may contribute to the development of post-infection irritable bowel syndrome (PI-IBS). The study demonstrated that certain Campylobacter genotypes exhibit increased virulence in vitro, shedding light on the complex host-pathogen interactions underlying this condition.
COMMUNICATIONS BIOLOGY
(2021)
Article
Microbiology
Emily Stoakes, George M. Savva, Ruby Coates, Noemi Tejera, Mark G. Poolman, Andrew J. Grant, John Wain, Dipali Singh
Summary: This study investigated the substrate utilization and energy metabolism of non-growing Campylobacter jejuni. By integrating phenotypic microarrays and genome-scale metabolic modelling, the study found that C. jejuni can survive on various substrates and produce ATP through different metabolic mechanisms. The ability to utilize a wide range of substrates may contribute to C. jejuni's success in surviving in changing environments.
Article
Agriculture, Dairy & Animal Science
Gaia Casalino, Giancarlo Bozzo, Francesca Rita Dinardo, Francesco D'Amico, Michela Maria Dimuccio, Antonio Camarda, Edmondo Ceci, Diana Romito, Elena Circella
Summary: This study evaluated the incidence of C. jejuni and C. coli infection in laying hens housed in different rearing systems, with higher infection rates found in hens housed in aviaries. Antibiotic resistance was mainly detected among C. coli strains, highlighting the need to increase biosecurity measures.
Article
Immunology
Brandon Ruddell, Alan Hassall, Orhan Sahin, Qijing Zhang, Paul J. Plummer, Amanda J. Kreuder
Summary: The genes metA and metB are essential for l-methionine production in Campylobacter jejuni in the absence of luxS, and play a critical role in normal chicken colonization by the bacteria.
INFECTION AND IMMUNITY
(2021)
Article
Food Science & Technology
Juan Wang, Zhi Wang, Jumei Zhang, Yu Ding, Zonghao Ma, Fufeng Jiang, Xiang Nie, Shengjun Tang, Moutong Chen, Shi Wu, Haiyan Zeng, Tao Lei, Xiaojuan Yang, Shuhong Zhang, Qingping Wu
Summary: Campylobacter jejuni isolated from retail food in China showed high resistance to vancomycin, cefoperazone, and quinolones, but sensitivity to erythromycin. The strains exhibited genetic diversity with the presence of new sequence types, and some strains were capable of forming strong biofilms.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Clinical Neurology
Ana P. Ramos, Sonja E. Leonhard, Susan K. Halstead, Mireya A. Cuba, Carlos C. Castaneda, Jose A. Dioses, Martin A. Tipismana, Jesus T. Abanto, Alejandro Llanos, Dawn Gourlay, Max Grogl, Mariana Ramos, Jesus D. Rojas, Rina Meza, Daniela Puiu, Rachel M. Sherman, Steven L. Salzberg, Patricia J. Simner, Hugh J. Willison, Bart C. Jacobs, David R. Cornblath, Hugo F. Umeres, Carlos A. Pardo
Summary: The study found that the 2019 Peruvian GBS outbreak was associated with Campylobacter jejuni infection, with the related strains circulating widely worldwide.
NEUROLOGY-NEUROIMMUNOLOGY & NEUROINFLAMMATION
(2021)
Article
Microbiology
Januana S. S. Teixeira, Valerie F. F. Boras, Benjamin M. M. Hetman, Eduardo N. N. Taboada, G. Douglas Inglis
Summary: This study found that in an agricultural ecosystem with high cattle density, cattle are the main source of Campylobacter infection, far exceeding chickens. This has important implications for reducing the burden of campylobacteriosis in the study location and elsewhere.
Article
Multidisciplinary Sciences
Canh Phung, Timothy B. Wilson, Jose A. Quinteros, Peter C. Scott, Robert J. Moore, Thi Thu Hao Van
Summary: Campylobacter hepaticus causes Spotty Liver Disease in chickens, and this study developed an enhanced liquid culture method for the efficient production of bacterial biomass. The addition of l-cysteine, l-glutamine, and sodium pyruvate significantly increased C. hepaticus growth, leading to nearly a tenfold increase in culture density.
SCIENTIFIC REPORTS
(2021)
Article
Microbiology
Chenyang Li, Jayaseelan Murugaiyan, Christian Thomas, Thomas Alter, Carolin Riedel
FRONTIERS IN MICROBIOLOGY
(2020)
Review
Agriculture, Dairy & Animal Science
Leonid Ushanov, Besarion Lasareishvili, Irakli Janashia, Andreas E. Zautner
Article
Medicine, General & Internal
Anja Doerschug, Julian Schwanbeck, Andreas Hahn, Anke Hillebrecht, Sabine Blaschke, Kemal Mese, Uwe Gross, Sascha Dierks, Hagen Frickmann, Andreas E. Zautner
Summary: Serological assays can be used to estimate the population's exposure to the SARS-CoV-2 virus, with varying sensitivity and specificity observed in different diagnostic assays for SARS-CoV-2-specific antibodies. Immunoglobulin G showed the best results in terms of sensitivity and agreement between different assays.
Article
Medicine, General & Internal
Anja Doerschug, Hagen Frickmann, Julian Schwanbeck, Elif Yilmaz, Kemal Mese, Andreas Hahn, Uwe Gross, Andreas E. Zautner
Summary: This study evaluated the impact of an RNA vaccine on antibody detection results, revealing the characteristics of antibody response post-vaccination, and suggesting that the simultaneous application of anti-spike protein and anti-nucleocapsid serological tests is an effective way to differentiate vaccine response and natural infection.
Article
Microbiology
Julian Schwanbeck, Wolfgang Bohne, Ufuk Hasdemir, Uwe Gross, Yvonne Pfeifer, Boyke Bunk, Thomas Riedel, Cathrin Sproeer, Jorg Overmann, Hagen Frickmann, Andreas E. Zautner
Summary: This study investigated the plasmid-resistome of seven bla(OXA-48) gene-carrying Klebsiella pneumoniae isolates in Germany, identifying different phenotypic resistance patterns associated with IncL/M and IncR plasmids among isolates of the ST101 cluster, indicating possible nosocomial transmission.
Article
Microbiology
Julian Schwanbeck, Ines Oehmig, Uwe Gross, Andreas E. Zautner, Wolfgang Bohne
Summary: The translation discusses the importance of flagellar motility for intestinal pathogens and highlights the swimming strategy of Clostridioides difficile at the single cell level and its dependence on environmental parameters.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Medicine, General & Internal
Sascha Dierks, Oliver Bader, Julian Schwanbeck, Uwe Gross, Michael S. Weig, Kemal Mese, Raimond Lugert, Wolfgang Bohne, Andreas Hahn, Nicolas Feltgen, Setare Torkieh, Fenja R. Denker, Peer Lauermann, Marcus W. Storch, Hagen Frickmann, Andreas Erich Zautner
Summary: This study compared the performance characteristics of two SARS-CoV-2-specific rapid antigen assays, as well as a transcription-mediated amplification (TMA) assay and real-time PCR. The results indicated that LumiraDx had higher sensitivity and specificity compared to NADAL antigen assay, and TMA testing was as accurate as PCR in a low prevalence setting.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Allergy
Moritz M. Hollstein, Lennart Muensterkoetter, Michael P. Schoen, Armin Bergmann, Thea M. Husar, Anna Abratis, Abass Eidizadeh, Meike Schaffrinski, Karolin Zachmann, Anne Schmitz, Jason S. Holsapple, Hedwig Stanisz-Bogeski, Julie Schanz, Andreas Fischer, Uwe Gross, Andreas Leha, Andreas E. Zautner, Moritz Schnelle, Luise Erpenbeck
Summary: This study found differences in humoral and cellular immune responses between different SARS-CoV-2 vaccination regimes (homologous and heterologous). Booster vaccination with BNT162b2 was more effective than ChAdOx1 nCoV-19, and no booster effect on T-cell activation was observed in the ChAdOx1 nCoV-19/BNT162b2 group.
Article
Virology
Lennart Muensterkoetter, Moritz Maximilian Hollstein, Andreas Hahn, Andrea Kroeger, Moritz Schnelle, Luise Erpenbeck, Uwe Gross, Hagen Frickmann, Andreas Erich Zautner
Summary: In this study, the anti-SARS-CoV-2 surrogate neutralization assays by TECOmedical and DiaPROPH-Med were evaluated, with TECOmedical showing a sensitivity of 100% and specificity of 61.3%, and DiaPROPH-Med showing a sensitivity of 95.0% and specificity of 48.4%. Furthermore, differences in the likelihood of expressing neutralizing antibodies were observed based on the number of vaccine doses and the type of exposure.
Article
Immunology
Clemens Grimm, Steffen Dickel, Julian Grundmann, Didier Payen, Julie Schanz, Andreas Erich Zautner, Bjoern Tampe, Onnen Moerer, Martin Sebastian Winkler
Summary: This study reported a case of multidrug-resistant superinfected COVID-19 acute respiratory distress syndrome. The immune system was successfully reactivated using IFN gamma therapy, leading to rapid resolution of infection and discharge of the patient.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Genetics & Heredity
Anastasia-Lisa Dieckmann, Thomas Riedel, Boyke Bunk, Cathrin Sproeer, Joerg Overmann, Uwe Gross, Oliver Bader, Wolfgang Bohne, Burkhard Morgenstern, Morteza Hosseini, Andreas E. Zautner
Summary: The recent discovery of mixed Campylobacter coli strains suggests that C. coli and C. jejuni may be going through a process of accelerated gene transfer termed 'despeciation'. The phylogenetic tree revealed a separate cluster of Campylobacter isolates that possibly indicates genetic introgression. These hybrid strains, resulting from genetic introgression between C. coli and C. jejuni, have a genomic mosaic structure influenced by C. jejuni.
MICROBIAL GENOMICS
(2021)
Article
Microbiology
Andreas Erich Zautner, Hagen Frickmann, Andreas Podbielski
Summary: Environmental investigation was conducted to guide exposure prevention for a peritoneal dialysis patient with transient colonization of her nostrils by Lichtheimia corymbifera. Although the mold itself was not grown from the collected samples, targeted recommendations were made based on systematic screening for molds in the air and on surfaces. Such investigations can help derive preventive recommendations based on the environmental distribution of molds.
Letter
Allergy
Moritz M. Hollstein, Lennart Muensterkoetter, Michael P. Schoen, Armin Bergmann, Thea M. Husar, Anna Abratis, Abass Eidizadeh, Sascha Dierks, Meike Schaffrinski, Karolin Zachmann, Anne Schmitz, Jason S. Holsapple, Hedwig Stanisz-Bogeski, Julie Schanz, Andreas Fischer, Uwe Gross, Andreas Leha, Andreas E. Zautner, Moritz Schnelle, Luise Erpenbeck
Article
Biotechnology & Applied Microbiology
Thi Thu Tra Vu, Thi Thu Ha Hoang, Susanne Fleischmann, Hong Ngan Pham, Thi Lan Huong Lai, Thi Thu Ha Cam, Lan Oanh Truong, Van Phan Le, Dac Cam Phung, Thomas Alter
Summary: This study reveals a high prevalence of V. parahaemolyticus in retail seafood in Hanoi, with a significant quantitative load and antimicrobial resistance.
JOURNAL OF FOOD PROTECTION
(2022)
Article
Microbiology
Yulan Su, Thomas Alter, Greta Goelz
Summary: The aim of this study was to investigate the impact of surrounding viscosity on the expression of motility related genes in Campylobacter jejuni. RNA was extracted from liquid cultures and bacterial cells recovered from the edge and center of a swarming halo. The study found different gene expression patterns and higher energy demand in high viscous media compared to liquid culture, indicating the importance of considering viscosity in motility related studies.
EUROPEAN JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY
(2023)