Article
Microbiology
Hermenegildo Taboada-Castro, Jeovanis Gil, Leopoldo Gomez-Caudillo, Juan Miguel Escorcia-Rodriguez, Julio Augusto Freyre-Gonzalez, Sergio Encarnacion-Guevara
Summary: A comparative proteomic study was conducted to investigate the metabolic pathways and transcriptional regulatory networks of Rhizobium under different growth conditions. The results show that Rhizobium is able to adapt to different conditions and has distinct transcriptional regulation. These findings are important for a better understanding of the metabolic pathways and transcriptional regulation mechanism in Rhizobium.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Jesus Lirio-Paredes, Katty Ogata-Gutierrez, Doris Zuniga-Davila
Summary: The study isolated Rhizobium strains from soil samples collected from coffee fields, and inoculated them on red kidney beans. The results showed that the strain RZC12 significantly improved plant growth, development, and yield of bean plants.
Article
Multidisciplinary Sciences
O. Mario Aguilar, Monica M. Collavino, Ulises Mancini
Summary: This study investigated the symbiotic affinity between common beans and rhizobia. It found that beans from Mesoamerica and the southern Andes have a strong affinity for rhizobial strains from Mexico and Ecuador, while beans from Ecuador-Peru and the Andean region show no affinity for sympatric rhizobial strains. Additionally, genetic variations associated with nodulation competitiveness were observed.
SCIENTIFIC REPORTS
(2022)
Article
Biotechnology & Applied Microbiology
Ivana Rajnovic, Martha-Helena Ramirez-Bahena, Sanja Kajic, Jose M. Igual, Alvaro Peix, Encarna Velazquez, Sanja Sikora
Summary: This study identified a symbiotic relationship between nodulated legume and strains of genus Rhizobium in Croatia. The analysis revealed that these strains are closely related to species from the Rhizobium leguminosarum phylogenetic complex. Based on genetic and phenotypic analyses, two novel species within the genus Rhizobium were proposed and named Rhizobium croatiense and Rhizobium redzepovicii.
SYSTEMATIC AND APPLIED MICROBIOLOGY
(2022)
Article
Plant Sciences
Rocio Reyero-Saavedra, Sara Isabel Fuentes, Alfonso Leija, Gladys Jimenez-Nopala, Pablo Pelaez, Mario Ramirez, Lourdes Girard, Timothy G. Porch, Georgina Hernandez
Summary: This study aims to isolate and characterize symbiotic mutants in common bean (Phaseolus vulgaris) in order to understand the symbiotic nitrogen fixation (SNF) process in this important food crop. Three non-nodulating mutants were identified and their impairments in different symbiotic steps were characterized. The responsible mutated gene is being mapped for further understanding of SNF in common bean.
Article
Agronomy
Fernando de Souza Buzo, Lucas Martins Gare, Nayara Fernanda Siviero Garcia, Maura Santos Reis de Andrade da Silva, Pedro Henrique Giova da Silva, Pamela Roberta de Souza Morita, Juliana Barboza Correa, Juliana Trindade Martins, Everlon Cid Rigobelo, Amaia Nogales, Orivaldo Arf
Summary: Chemical seed treatment and mycorrhizal inoculation benefit bean plants and their nutritional status. The best combinations for the bean crop were metalaxyl + fludioxonil + tiabendazole with 41.4 mg of the inoculant per 100 seeds and pyraclostrobin + thiophanate methyl + fipronil with 62.1 mg of the inoculant per 100 seeds.
PEST MANAGEMENT SCIENCE
(2022)
Article
Biology
Bruna Fernanda Silva De Sousa, Lucia Domingo-Serrano, Alvaro Salinero-Lanzarote, Jose Manuel Palacios, Luis Rey
Summary: The genes of the T6SS system in Rhizobium etli Mim1 were divided into three modules, which were found to be unnecessary for effective nodulation with beans. The expression of the T6SS system was observed to be lower in symbiosis compared to free living. The secretion of Re78 protein required an active T6SS system, and Re78 and Re79 proteins were identified as a toxic effector/immunity protein pair.
Article
Biology
Daniel Perez-Mendoza, Lorena Romero-Jimenez, Miguel Angel Rodriguez-Carvajal, Maria J. Lorite, Socorro Munoz, Adela Olmedilla, Juan Sanjuan
Summary: Bacterial exopolysaccharides play important roles in bacterial survival, colonization, and host-microbe interactions. Rhizobium etli is the first bacterium capable of producing cellulose and mixed linkage beta-glucan. The production of these polysaccharides is regulated by c-di-GMP levels and contributes to the interaction between the bacterium and its host.
Article
Microbiology
Jenjira Wongdee, Pongdet Piromyou, Pongpan Songwattana, Teerana Greetatorn, Neung Teaumroong, Nantakorn Boonkerd, Eric Giraud, Nico Nouwen, Panlada Tittabutr
Summary: RpoN is an alternative sigma factor that recruits the core RNA polymerase to gene promoters. It has diverse physiological functions in bacteria, including a key role in nitrogen fixation genes. In the Bradyrhizobium sp. DOA9 strain, both the chromosomal and plasmid encoded RpoN proteins have pleiotropic effects on the physiology of the bacteria under free-living and symbiotic conditions. Inactivation of these proteins severely impacts bacterial motility, carbon and nitrogen utilization, EPS production, and biofilm formation under free-living conditions, and also reduces symbiotic nodulation, nitrogen fixation efficiency, and intracellular survival ability.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Plant Sciences
Raul Davila-Delgado, Karen Flores-Canul, Marco Adan Juarez-Verdayes, Rosana Sanchez-Lopez
Summary: PvSYMRK-EGFP undergoes constitutive and rhizobia-induced endocytosis, which is regulated by the phosphorylation status, the endocytic motif YXXo, and the kinase activity of the receptor. These findings suggest that rhizobia-induced endocytosis modulates the duration and amplitude of the SYMRK signaling pathway.
Article
Microbiology
Francisco Fuentes-Romero, Pilar Navarro-Gomez, Paula Ayala-Garcia, Isamar Moyano-Bravo, Francisco-Javier Lopez-Baena, Francisco Perez-Montano, Francisco-Javier Ollero-Marquez, Sebastian Acosta-Jurado, Jose-Maria Vinardell
Summary: This study demonstrates that the nodD1 gene of Sinorhizobium fredii HH103 can restore the nodulation capacity of a double nodD1/nodD2 mutant of Rhizobium tropici CIAT 899 in bean plants. Mutation of certain symbiotic regulatory genes affects the symbiotic behavior and nitrogenase activity of HH103, leading to the formation of colonized nodules.
Article
Plant Sciences
Arantza del-Canto, Alvaro Sanz-Saez, Anna Sillero-Martinez, Eider Mintegi, Maite Lacuesta
Summary: Drought is a major challenge in agriculture due to its negative impact on crop growth and yield, but inoculating pulses with rhizobium strains can improve drought tolerance. This study evaluated the ability of indigenous rhizobia strains from nearby crop fields to enhance drought tolerance in common bean plants. Three strains were found to be highly efficient under drought conditions, showing better infectiveness and effectiveness compared to reference strains and chemically fertilized plants. These results provide a basis for developing efficient common bean inoculants to increase crop yield under drought conditions in Northern Spain.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Genetics & Heredity
Carolina Cervera-Torres, Manoj-Kumar Arthikala, Miguel Lara, Lourdes Blanco, Kalpana Nanjareddy
Summary: In this study, a comprehensive analysis of the PvSnRK gene family in Phaseolus vulgaris was conducted. The results showed that PvSnRK genes play an important regulatory role in symbiosis, as well as hormones and abiotic stresses. The analysis of gene structures and sequence conservation revealed the diversity and high conservation of the PvSnRK gene family.
Article
Plant Sciences
Karima F. Abdelgawad, Asmaa H. R. Awad, Marwa R. Ali, Richard A. Ludlow, Tong Chen, Mohamed M. El-Mogy
Summary: This study found that the combination of chitosan with tea tree oil, peppermint oil, or ascorbic acid can reduce the quality loss of fresh-cut green bean pods during storage, maintaining weight and firmness, and increasing nutrient content and antioxidant capacity. The combination of chitosan and ascorbic acid showed the best effect.
Article
Biotechnology & Applied Microbiology
Sameh H. Youseif, Fayrouz H. Abd El-Megeed, Akram H. Mohamed, Amr Ageez, Esteban Veliz, Esperanza Martinez-Romero
Summary: In this study, 80 rhizobial isolates were obtained from berseem clover root nodules in Egypt, most of which were closely related to Rhizobium aegyptiacum and Rhizobium aethiopicum, showing superior nodulation and nitrogen fixation capabilities. Some strains were also affiliated to other rhizobial species, indicating a diverse symbiotic relationship with berseem clover.
SYSTEMATIC AND APPLIED MICROBIOLOGY
(2021)
Article
Biochemical Research Methods
Oscar Gerardo Silva-Gaona, Juan Manuel Guzman-Flores, Magdalena Hernandez-Ortiz, Katya Vargas-Ortiz, Joel Ramirez-Emiliano, Sergio Encarnacion-Guevara, Victoriano Perez-Vazquez
Summary: The study found that curcumin can reverse the expression changes of seven proteins in the liver of diabetic mice, which are involved in the metabolism of amino acids, carbohydrates, and lipids.
CURRENT PROTEOMICS
(2022)
Article
Microbiology
Isaac B. Olivar-Casique, Liliana Medina-Aparicio, Selena Mayo, Yitzel Gama-Martinez, Javier E. Rebollar-Flores, Gabriel Martinez-Batallar, Sergio Encarnacion, Edmundo Calva, Ismael Hernandez-Lucas
Summary: Salmonella enterica serovar Typhi uses multiple genetic elements to resist the presence of human bile, and enhances energy production to sustain damaged cell envelope components.
JOURNAL OF MEDICAL MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Karla N. Valenzuela-Valderas, Gabriel Moreno-Hagelsieb, John R. Rohde, Rafael A. Garduno
Summary: Research has shown that the bacterial pathogen Legionella pneumophila's chaperonin protein HtpB has a unique function in interaction with the eukaryotic protein ECM29, which can be mapped to specific amino acid positions.
Article
Pharmacology & Pharmacy
Maria Cristina Leon-Garcia, Oscar Gerardo Silva-Gaona, Magdalena Hernandez-Ortiz, Katya Vargas-Ortiz, Joel Ramirez-Emiliano, Ma Eugenia Garay-Sevilla, Sergio Encarnacion-Guevara, Victoriano Perez-Vazquez
Summary: The study demonstrated that curcumin could reduce the glycation levels of specific proteins in the hearts of mice fed with a high fructose diet, which are involved in various biological processes such as cellular respiration and oxidative phosphorylation.
CURRENT PHARMACEUTICAL DESIGN
(2022)
Article
Genetics & Heredity
Victor H. Tierrafria, Claire Rioualen, Heladia Salgado, Paloma Lara, Socorro Gama-Castro, Patrick Lally, Laura Gomez-Romero, Pablo Pena-Loredo, Andres G. Lopez-Almazo, Gabriel Alarcon-Carranza, Felipe Betancourt-Figueroa, Shirley Alquicira-Hernandez, J. Enrique Polanco-Morelos, Jair Garcia-Sotelo, Estefani Gaytan-Nunez, Carlos-Francisco Mendez-Cruz, Luis J. Muniz, Cesar Bonavides-Martinez, Gabriel Moreno-Hagelsieb, James E. Galagan, Joseph T. Wade, Julio Collado-Vides
Summary: This paper presents a radical upgrade of RegulonDB, offering more than 2000 high-throughput datasets on transcriptional regulation. The datasets have been processed for comparison and include information on transcription termination sites, transcription units, transcription factor binding interactions, and expression profiles. The browsing and visualization tools have also been expanded, allowing comparisons against previous knowledge, genome viewing, and metadata querying.
MICROBIAL GENOMICS
(2022)
Article
Biochemical Research Methods
Oscar Gerardo Silva-Gaona, Magdalena Hernandaz-Ortiz, Katya Vargas-Ortiz, Joel Ramirez-Emiliano, Ma. Eugenia Garay-Sevilla, Sergio Encarnacion-Guevara, Victoriano Perez-Vazquez
Summary: Increased fructose consumption is associated with metabolic diseases. Curcumin, a natural compound, can regulate protein expression and metabolic pathways. This study found that the expression of 113 proteins changed in the livers of mice fed fructose, and curcumin modified the expression of 64 proteins in mice fed fructose and curcumin. Curcumin can prevent some protein expression changes induced by fructose consumption.
JOURNAL OF PROTEOMICS
(2022)
Article
Microbiology
Jonathan Padilla-Gomez, Roberto Jhonatan Olea-Ozuna, Sandra Contreras-Martinez, Orlando Morales-Tarre, Daniela A. Garcia-Soriano, Diana X. Sahonero-Canavesi, Sebastian Poggio, Sergio Encarnacion-Guevara, Isabel M. Lopez-Lara, Otto Geiger
Summary: This study reveals that in Rhodobacteria, the structural gene for serine palmitoyltransferase (SPT) co-occurs with genes coding for a putative acyl carrier protein (ACP) and a putative acyl-CoA synthetase (ACS). It is found that the putative ACP is a specialized ACP (AcpR) required for sphingolipid biosynthesis, and the putative ACS is a specialized acyl-ACP synthetase (AasR). The study also shows that acyl-AcpR is used as a substrate for the synthesis of 3-oxo-sphinganine.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Maria Luisa Castrejon-Godinez, Efrain Tovar-Sanchez, Ma Laura Ortiz-Hernandez, Sergio Encarnacion-Guevara, Angel Gabriel Martinez-Batallar, Magdalena Hernandez-Ortiz, Enrique Sanchez-Salinas, Alexis Rodriguez, Patricia Mussali-Galante
Summary: Methyl parathion, a widely used pesticide, poses a significant threat to ecosystems and human health due to its high toxicity and persistence in the environment. Identifying microorganisms capable of degrading methyl parathion is crucial for bioremediation efforts at pesticide-polluted sites.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2022)
Article
Microbiology
Hermenegildo Taboada-Castro, Jeovanis Gil, Leopoldo Gomez-Caudillo, Juan Miguel Escorcia-Rodriguez, Julio Augusto Freyre-Gonzalez, Sergio Encarnacion-Guevara
Summary: A comparative proteomic study was conducted to investigate the metabolic pathways and transcriptional regulatory networks of Rhizobium under different growth conditions. The results show that Rhizobium is able to adapt to different conditions and has distinct transcriptional regulation. These findings are important for a better understanding of the metabolic pathways and transcriptional regulation mechanism in Rhizobium.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biology
Maria J. Lorite, Ariana Casas-Roman, Lourdes Girard, Sergio Encarnacion, Natalia Diaz-Garrido, Josefa Badia, Laura Baldoma, Daniel Perez-Mendoza, Juan Sanjuan
Summary: The second messenger cyclic diguanylate (cdG) is known to play a crucial role in biofilm formation in bacteria. This study found that high levels of cdG not only promote the secretion of proteins involved in adhesion and biofilm formation, but also facilitate the export of cytoplasmic proteins. These cytoplasmic proteins have been previously described as moonlighting or multifunctional proteins, often found extracellularly or at the bacterial cell surface. The findings suggest that cdG promotes the active exportation of cytoplasmic proteins through yet unknown mechanisms involving post-translational modification.
Article
Pharmacology & Pharmacy
Marcos H. Salazar, Magdalena Hernandez Ortiz, Sergio Encarnacion, Fernando Zamudio, Lourival D. Possani, John Cleghorn, Maricruz Moran, Hildaura Acosta, Gerardo Corzo
Summary: In recent years, there has been an increase in morbidity caused by Tityus championi scorpion sting in Panama. The LD50 of the venom was determined to be 2.9 mg/kg through intravenous injection. Toxic fractions of the venom were identified and tested in mice to find the most toxic fractions to mammals. Additionally, the venom fractions were tested against invertebrates to search for insect-specific toxin peptides. The primary structures of the most relevant venom peptides were obtained, with one of the neurotoxic peptides being found in at least four other Tityus species in Panama. This neurotoxin could be an important protein target for developing antivenoms against the sting of this Panamanian scorpion or other relevant Tityus species.
Article
Food Science & Technology
Ivan Jalil Anton Carreno-Marquez, Rene Renato Balandran-Quintana, Jose Antonio Azamar-Barrios, Jose Angel Huerta-Ocampo, Ana Maria Mendoza-Wilson, Gabriela Ramos-Clamont Montfort, Sergio Encarnacion-Guevara, Magdalena Hernandez-Ortiz
Summary: Wheat bran aqueous extracts contribute to the synthesis of spherical microparticles of dicalcium phosphate dihydrate or Brushite, which has potential applications in the food industry. Proteins in the extract were identified as scaffold biomolecules for the growth of Brushite crystals. Size exclusion chromatography revealed specific fractions responsible for Brushite synthesis. Results suggest that certain proteins in the wheat bran aqueous extract play a role as a scaffold in spherical Brushite biomineralization.
Article
Microbiology
Lucia Jimenez, Mariana Diaz-Zaragoza, Magdalena Hernandez, Luz Navarro, Ricardo Hernandez-Avila, Sergio Encarnacion-Guevara, Pedro Ostoa-Saloma, Abraham Landa
Summary: A study on a cysticercosis model of Taenia crassiceps ORF strain in mice found that susceptible mice showed a Th2 response promoting parasite growth, while resistant mice showed a sustained Th1 response limiting parasite growth. Proteomics analysis revealed 128 expressed proteins in the parasite under the Th1 environment in resistant mice, with 11 proteins increasing expression at 4 weeks and decreasing at 8 weeks. These proteins participate in tissue repair, immunoregulation, and parasite establishment, indicating their potential as drug or vaccine targets.
Article
Multidisciplinary Sciences
Xin Wei, Gabriel Moreno-Hagelsieb, Bernard R. Glick, Andrew C. Doxey
Summary: Cytokinin, the plant hormone used in agriculture, is synthesized by the enzyme Isopentenyl transferase (IPT). Comparisons of IPT genes between plant pathogens and plant growth-promoting bacteria (PGPB) show differences in gene structure, regulation, and activity levels, which may determine their effects on host plants.
Article
Multidisciplinary Sciences
Julie E. Hernandez-Salmeron, Tanya Irani, Gabriel Moreno-Hagelsieb
Summary: Average Nucleotide Identity (ANI) is a standard measure for bacterial species delimitation, but its calculation time is often longer than similarity estimates based on short nucleotide sampling. In this study, two sketching programs, mash and dashing, were compared to ANI in delimiting species among Esterobacterales genomes. The results showed that fast estimates of genome similarity were as accurate as ANI for species delimitation, indicating their potential use in efficient bacterial identification and clustering.
