Article
Biotechnology & Applied Microbiology
Hao Zhang, Zhilin Ouyang, Nannan Zhao, Shuangyan Han, Suiping Zheng
Summary: In this study, the AmtR regulon in C. glutamicum ATCC 14067 was identified using ChIP-seq, revealing new operons related to creatine and creatinine degradation. The negative regulation of transcription and expression of these genes by AmtR was confirmed. Additionally, it was found that C. glutamicum ATCC 14067 can utilize creatine or creatinine as a sole nitrogen source.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Qiaoyun Zhu, Qi Liu, Chaoying Yao, Yuanxing Zhang, Menghao Cai
Summary: In this study, yeast transcriptional device libraries were developed for precise synthesis of value-added chemicals from methanol. The transcriptional devices showed good activity and expression strength in a methylotrophic yeast. By selecting and optimizing the devices, the production of chemicals from methanol can be significantly increased.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Biotechnology & Applied Microbiology
Tao Su, Chengchuan Che, Jiyu Han, Yuying Zhao, Zihan Zhang, Guangdi An, Meiru Si, Can Chen
Summary: The study reveals that AtsR is a redox regulator that senses oxidative stress and can directly repress and indirectly regulate the expression of related genes.
MICROBIAL CELL FACTORIES
(2022)
Article
Biochemistry & Molecular Biology
Soong-bin Kang, Jong-il Choi
Summary: The introduction of the response regulator DR1558 successfully enhanced the production of cadaverine in C. glutamicum under acidic conditions, indicating its impact on gene expression and stress defense mechanisms.
PROCESS BIOCHEMISTRY
(2021)
Article
Engineering, Chemical
Zaiwei Man, Jin Li, Huihui Cui, Zhiqiang Cai, Jing Guo
Summary: This study optimized the L-citrulline biosynthesis operon in C. glutamicum and successfully constructed an efficient L-citrulline-producing strain. The L-citrulline production was further improved by plasmid-based overexpression of the optimized operon.
Article
Agricultural Engineering
An Jiang, Yunhai Song, Jia You, Xian Zhang, Meijuan Xu, Zhiming Rao
Summary: Efficient production of ectoine in Corynebacterium glutamicum was achieved through metabolic engineering and plug-in repressor library strategy, providing guidance for the construction of microbial cell factories.
BIORESOURCE TECHNOLOGY
(2022)
Article
Microbiology
Jiaxun Zhang, Lihua Hu, Hua Zhang, Zheng-Guo He
Summary: In this study, we discovered a new mechanism of hypoxic adaptation in M. bovis driven by the second messenger molecule c-di-GMP. We found that c-di-GMP accumulates in bacterial cells under hypoxic stress, blocking the inhibitory activity of ArgR and promoting arginine synthesis and nitrite respiration. These findings provide a potential new target for blocking the life cycle of M. tuberculosis infection.
ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Microbiology
Nadja A. Henke, Irene Krahn, Volker F. Wendisch
Summary: This study successfully achieved strong and overexpression of target genes by designing new expression vectors and combining two strategies, providing new possibilities for the development of biotechnology applications.
Article
Biochemical Research Methods
Yanting Duan, Weiji Zhai, Weijia Liu, Xiaomei Zhang, Jin-Song Shi, Xiaojuan Zhang, Zhenghong Xu
Summary: In this research, 17 core promoters and 29 RBSs in Corynebacterium glutamicum were characterized, showing significant differences in transcriptional and translational activity. The study revealed that RBSs contribute more to gene expression than promoters, but their efficacy is influenced by gene context, indicating poor orthogonality.
ACS SYNTHETIC BIOLOGY
(2021)
Article
Microbiology
Tobias Busche, Hana Dostalova, Lenka Rucka, Jiri Holatko, Ivan Barvik, Vaclav Stepanek, Miroslav Patek, Joern Kalinowski
Summary: The sigma H and sigma E subunits of Corynebacterium glutamicum RNA polymerase belong to Group 4 of sigma factors, also called extracytoplasmic function (ECF) sigma factors. While the genes of the C. glutamicum sigma(Eta) regulon involved in heat and oxidative stress response have been studied, the genes of the sigma(E) regulon, involved in cell surface stress response, have not been explored until now. Differential gene expression analysis revealed a significant overlap between the sigma(Eta) and sigma(E) regulons, and identified genes involved in protein quality control, regulation of Clp proteases, and membrane integrity maintenance.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Koichi Toyoda, Masayuki Inui
Summary: When transitioning from exponential to stationary growth phases, bacterial metabolism shifts from aerobic respiration to fermentation in response to limited oxygen availability. The ldhA gene in Corynebacterium glutamicum encodes l-lactate dehydrogenase, responsible for l-lactate production, and is controlled by transcription regulators SugR, LldR, and GlxR. GlxR has been shown to act as a transcriptional activator of ldhA, affecting both its promoter activity and l-lactate production.
Article
Microbiology
Jianxiao Zhao, Xi Sun, Zhitao Mao, Yangyang Zheng, Zhouxiao Geng, Yuhan Zhang, Hongwu Ma, Zhiwen Wang
Summary: Gene expression in bacteria is regulated by multiple transcription factors. In this study, we used independent component analysis to analyze the gene expression profile of Corynebacterium glutamicum and constructed a quantitative transcriptional regulatory network. We identified potential targets for transcription factors and observed changes in the network under different growth and environmental conditions.
MICROBIOLOGICAL RESEARCH
(2023)
Article
Biotechnology & Applied Microbiology
Nannan Zhao, Jian Wang, Aiqing Jia, Ying Lin, Suiping Zheng
Summary: A putrescine-specific biosensor (pSenPuuR) was developed in Corynebacterium glutamicum using Escherichia coli-derived transcriptional factor PuuR. The response characteristics of the biosensor to putrescine were improved by optimizing the genetic components of pSenPuuR. The study suggests that pSenPuuR has the potential to assess putrescine production in C. glutamicum and is suitable for high-throughput genetic variant screening.
BIOENGINEERING-BASEL
(2023)
Article
Biochemistry & Molecular Biology
Aigera Mendauletova, John A. Latham
Summary: This study investigates the regulation mechanisms of Mycofactocin (MFT) expression in Mycobacterium smegmatis mc(2)155. It is found that the transcription regulator, MftR, binds to the mft promoter region and is induced by long-chain acylCoAs. The results suggest that MFT may play a critical role in fatty acid metabolism or cell wall reorganization.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Microbiology
Jingwen Huang, Jiuzhou Chen, Yu Wang, Tuo Shi, Xiaomeng Ni, Wei Pu, Jiao Liu, Yingyu Zhou, Ningyun Cai, Shuangyan Han, Ping Zheng, Jibin Sun
Summary: By developing a hyperosmotic stress inducible gene expression system, successful gene auto-regulation was achieved in C. glutamicum, significantly increasing lysine production.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Energy & Fuels
Soo-Jin Han, Jin-Soo Park
Summary: The study investigates the fouling tendency of positively charged, negatively charged, and non-charged surfactants on ion-exchange membranes. It is found that charged foulants in seawater could significantly foul the membranes, while the worst fouling behavior is observed when charged foulants are present in river streams. Through zeta potential measurement, it is demonstrated that the Debye length of charged foulants decreases in seawater streams due to higher ionic strength, leading to lower net electrostatic effect and less fouling tendency in reverse electrodialysis.
Article
Materials Science, Multidisciplinary
Jongkook Hwang, Jin Hyung Lee, Jinyoung Chun
Summary: The study introduced a facile synthetic method to prepare spherical mesoporous silica nanoparticles with tunable mesopores, utilizing acetic acid and PEG as key components to control the particle size and morphology.
Article
Energy & Fuels
Yang Mo Gu, Seon Young Park, Ji Yeon Park, Byoung-In Sang, Byoung Seong Jeon, Hyunook Kim, Jin Hyung Lee
Summary: Attrition ball-mill pretreatment of food waste showed improvements in particle size uniformity, soluble chemical oxygen demand (SCOD), biochemical methane potential, and total volatile fatty acids. Increasing milling speed and time led to higher SCOD and methane production, but extended milling time did not significantly improve the methane yield. Additionally, the microbial community analysis revealed a shift towards acetoclastic methanogens dominance and higher relative abundance of hydrogenotrophic methanogens in the pretreated samples.
Article
Biochemistry & Molecular Biology
Chan Hee Lee, Hye Sun Lee, Jae Won Lee, Jangyong Kim, Jin Hyung Lee, Eon Seon Jin, Ee Taek Hwang
Summary: Enzyme immobilization using inorganic materials, such as CaCO3, has been shown to improve enzyme stability and activity. In this study, in situ and crosslink-immobilized enzymes in a CaCO3 carrier were compared, with both methods preserving over 30% of initial enzyme activity after 180 days. However, differences in enzyme loading, activity, and characteristics were observed between the two methods.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Chemistry, Multidisciplinary
Prabhsharan Kaur, Da-Eun Kim, Gaurav Verma, Jin-Soo Park, Satpal Singh Sekhon
Summary: The surface modification of carbon nanomaterials with heteroatoms enhances their electrocatalytic activity for oxygen reduction reaction. PDDA and Triton X-100 have different effects on the surface modification of carbon nanofibers, affecting electron acceptance and intermolecular charge transfer. The conjugated systems exhibit higher efficiency and current density closer to platinum for oxygen reduction reaction.
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
(2021)
Article
Electrochemistry
Jong-Hyeok Park, Mun-Sik Shin, Jin-Soo Park
Summary: This study investigates the influence of solvents in ionomer dispersions on the performance and durability of catalyst layers in PEMFCs. It shows that using ethylene glycol based ionomer dispersions can lead to the formation of an optimal microstructure with thinner ionomer layer and crack-free surface, resulting in higher performance and durability for PEMFCs. This research provides valuable insights for selecting dispersing solvents for catalyst layers with enhanced performance and durability in PEMFCs.
ELECTROCHIMICA ACTA
(2021)
Article
Green & Sustainable Science & Technology
Ji Yeon Park, Yang Mo Gu, Seon Young Park, Ee Taek Hwang, Byoung-In Sang, Jinyoung Chun, Jin Hyung Lee
Summary: An improved silica extraction process from rice husk was developed using a two-stage continuous method, involving attrition ball milling and alkaline leaching. The optimum conditions for the continuous process were determined through investigations on alkaline leaching parameters. NaOH was found to yield slightly more silica compared to KOH. The two-stage continuous extraction process operated stably for 80 hours with an 89% silica yield, with the optimal conditions of 0.2 M reaction concentration, 80 degrees C temperature, 3 hours duration time, and 6% (w/v) solid content.
Article
Pharmacology & Pharmacy
Jin Sil Lee, Hyeryeon Oh, Daekyung Sung, Jin Hyung Lee, Won Il Choi
Summary: The use of thermosponge nanoparticles loaded with paclitaxel can improve its anticancer effects by increasing drug release rate, leading to significant anticancer activity against multidrug resistance-related colorectal cancer cells.
Article
Engineering, Chemical
Il-Seop Jang, Ji Yeon Park, Hayoung Park, Yang Mo Gu, Jin Hyung Lee, Jinyoung Chun
Summary: A facile method has been developed for synthesizing spherical silica microparticles from rice husks, with crucial steps including silicate extraction under mild conditions and precipitation with a polyethylene glycol additive. The porosity of the silica particles can be controlled by adjusting the calcination temperature.
CHEMICAL ENGINEERING & TECHNOLOGY
(2022)
Article
Energy & Fuels
Beom-Seok Kim, Jin-Soo Park
Summary: This study introduces three environmentally friendly fouling mitigation technologies and evaluates their effectiveness in RED. It was found that alternating periods of power generation and fouling mitigation over five cycles resulted in 1.7 times higher power generation compared to continuous power generation cycles.
Review
Chemistry, Applied
Satpal Singh Sekhon, Jaeyoung Lee, Jin-Soo Park
Summary: Oxygen electrode catalysts are crucial for energy technologies, and biomass-derived heteroatom-doped porous carbons have emerged as low-cost, renewable materials for efficient bifunctional oxygen catalysts. These materials show good activity and stability in both oxygen reduction and evolution reactions.
JOURNAL OF ENERGY CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Virginia Ghita Firsty, Ji Yeon Jeong, Yang Mo Gu, Jin Hyung Lee, Soo-Jeong Shin
Summary: The acid hydrolysis treatment of kenaf outer bast fiber can produce pure cellulose content and hydrolyzed hemicellulose to monosaccharides. The study investigated the effects of various reaction temperatures, acid concentrations, and reaction times to find the optimum conditions for obtaining pure cellulose content. The results showed that a higher reaction temperature of 130 degrees C coupled with a short reaction time of 60 min using 0.25 N sulfuric acid was optimal for the hydrolysis of hemicellulose, while a longer reaction time of 120 min with a high sulfuric acid concentration of 1.00 N was necessary for improving the purity of cellulose.
APPLIED SCIENCES-BASEL
(2023)
Article
Biochemistry & Molecular Biology
Mahamuda Akter, Jin-Soo Park
Summary: This study investigates the impact of surface roughness and hydrophilicity on membrane fouling using two types of ion exchange membranes. The commercially available membranes have rough and hydrophilic surfaces, while the lab-made membranes have smooth and hydrophobic surfaces. The fouling tests confirm that electrical attraction is the main cause of membrane fouling, with anionic and cationic foulants affecting anion and cation exchange membranes, respectively. The results show that a rough surface has a higher fouling tendency in short-term tests, but the fouling can be easily mitigated due to the hydrophilic surface. Conversely, a smooth surface initially reduces fouling, but leads to irreversible fouling due to the accumulation of foulants on the hydrophobic surface.
Article
Biochemistry & Molecular Biology
Beom-Seok Kim, Jong-Hyeok Park, Jin-Soo Park
Summary: In this study, blended PFSA ionomers with different equivalent weights were prepared and studied for their applications in fuel cells. The results showed that blended ionomers exhibited higher ion conductivity and proton conduction ability, resulting in improved fuel cell performance.
Article
Chemistry, Multidisciplinary
Seongseop Kim, Ji Yeon Park, Yang Mo Gu, Il-Seop Jang, Hayoung Park, Kyeong Keun Oh, Jin Hyung Lee, Jinyoung Chun
Summary: A method for obtaining size-controlled spherical silica from rice husk using an eco-friendly and simplified process has been developed. The silica particles obtained were uniformly spherical in shape and had a surface area of more than 200 m²/g. The use of polyethylene glycol additives during the precipitation step and temperature adjustment allowed for control of the size of the silica particles.
NANOSCALE ADVANCES
(2021)