Article
Biotechnology & Applied Microbiology
Taehee Han, Sang Yup Lee
Summary: The biosynthesis of Valerolactam (VL) was achieved by metabolically engineered Corynebacterium glutamicum, which overproduces L-lysine. This provides a green and sustainable method for the production of precursor chemicals for nylon-5 and nylon 6,5.
METABOLIC ENGINEERING
(2023)
Article
Biochemistry & Molecular Biology
Jinhui Niu, Zhitao Mao, Yufeng Mao, Ke Wu, Zhenkun Shi, Qianqian Yuan, Jingyi Cai, Hongwu Ma
Summary: The genome-scale enzyme-constrained model (ecCGL1) improves the prediction of phenotypes and simulates overflow metabolism in Corynebacterium glutamicum, while also recapitulating the trade-off between biomass yield and enzyme usage efficiency.
Article
Microbiology
Vanessa L. Goettl, Ina Schmitt, Kristina Braun, Petra Peters-Wendisch, Volker F. Wendisch, Nadja A. Henke
Summary: This study constructed a library for the repression of 74 genes of C. glutamicum using CRISPRi technology, targeting important genes involved in metabolic pathways. The results showed that CRISPRi-mediated gene repression can increase carotenoid pigment biosynthesis, while deletion of certain genes can significantly enhance pigment production.
Article
Biotechnology & Applied Microbiology
Lukas Jungmann, Sarah Lisa Hoffmann, Caroline Lang, Raphaela De Agazio, Judith Becker, Michael Kohlstedt, Christoph Wittmann
Summary: This study demonstrates the production of hydroxyectoine through metabolic engineering, using a genetically modified strain of C. glutamicum. The optimized strain achieved high yields of hydroxyectoine, showing potential for its use in the cosmetics and pharmaceutical industries as an active ingredient.
MICROBIAL CELL FACTORIES
(2022)
Review
Biotechnology & Applied Microbiology
Yan Jiang, Qi Sheng, Xiao-Yu Wu, Bang-Ce Ye, Bin Zhang
Summary: This review summarizes recent advances in molecular biology strategies for optimizing l-arginine-producing strains, including various metabolic engineering methods to reduce production costs, providing valuable insights into the current state of l-arginine production.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Cheng Li, Charles A. Swofford, Christian Ruckert, Anthony J. Sinskey
Summary: Optimizing the recombineering efficiency in Corynebacterium glutamicum by testing different recombinase/exonuclease pairs resulted in the highest efficiency reported in the literature, with 13,250 transformed colonies/10^9 viable cells achieved for dsDNA integration. This optimized system allows for the deletion of over 40,000 bp in one transformation step and will greatly improve the speed of genetic modifications in C. glutamicum.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Biochemical Research Methods
Meng Chai, Xianhao Xu, Yanfeng Liu, Jianghua Li, Guocheng Du, Xueqin Lv, Long Liu
Summary: In this study, a recombinant strain of Corynebacterium glutamicum was constructed for the efficient biosynthesis of L-Histidine. By genetic modification and optimization of energy status, the production of L-Histidine was significantly improved. This research provides an efficient cell factory for L-Histidine biosynthesis through combinatorial protein engineering and metabolic engineering.
ACS SYNTHETIC BIOLOGY
(2023)
Article
Agricultural Engineering
Jie Liu, Ying Ou, Jian-Zhong Xu, Zhi-Ming Rao, Wei-Guo Zhang
Summary: This study describes the systems metabolic engineering of Corynebacterium glutamicum to enhance L-lysine production. Key chromosomal mutations related to L-lysine synthesis were identified through whole-genome sequencing. Carbon flux was redirected to the L-lysine synthesis pathway, increasing energy availability and product transport systems. A promoter library sensitive to intracellular L-lysine concentration was constructed and used for dynamic regulation of the NADPH pool. In fed-batch fermentation, the engineered strain achieved a L-lysine titer of 223.4 ± 6.5 g/L. This study is the first to improve L-lysine production by enhancing ATP supply and NADPH self-regulation, improving intracellular conditions.
BIORESOURCE TECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Sung Sun Yim, Jae Woong Choi, Yong Jae Lee, Ki Jun Jeong
Summary: In this study, a metabolic rewiring strategy was employed to construct a heterologous PHB biosynthetic pathway in Corynebacterium glutamicum, resulting in enhanced production of PHB. A fluorescence-activated cell sorting (FACS)-based screening method was developed to quickly identify mutants with higher PHB production. This research will contribute to accelerating microbial engineering processes for the production of diverse biochemicals and biopolymers.
MICROBIAL CELL FACTORIES
(2023)
Article
Biotechnology & Applied Microbiology
Young Jin Ko, Minhye Kim, Seung Kyou You, Sang Kyu Shin, Joonhee Chang, Hae Jin Choi, Wu-Young Jeong, Myeong-Eun Lee, Dong-Hyeok Hwang, Sung Ok Han
Summary: The study demonstrated the development of engineered Corynebacterium glutamicum capable of high-yield production of heme, with a maximum titer of 309.18 mg/L, including secreted heme of 242.95 mg/L, which are the highest values reported to date. Various strategies including system metabolic engineering and modification of membrane surface were utilized to enhance heme production, making C. glutamicum a potential cell factory for animal-free heme production.
METABOLIC ENGINEERING
(2021)
Review
Biotechnology & Applied Microbiology
Shengzhu Yu, Bo Zheng, Zhenya Chen, Yi-Xin Huo
Summary: Branched chain amino acids have been synthesized through metabolic engineering in Corynebacterium glutamicum. Further research should focus on the feedback inhibition and transcription attenuation mechanisms of key enzymes, as well as the development of enzymes with substrate specificity for the production of individual BCAAs. The strategies used in constructing BCAAs-producing strains provide valuable guidance for the biosynthesis of other high value-added compounds.
MICROBIAL CELL FACTORIES
(2021)
Article
Biotechnology & Applied Microbiology
Zihua Li, Yufei Dong, Yu Liu, Xuecong Cen, Dehua Liu, Zhen Chen
Summary: In this study, an efficient C. glutamicum strain for the production of 1,3-propanediol (1,3-PDO) from glucose and xylose was developed using systems metabolic engineering approaches. Through the construction and optimization of glycerol and 1,3-PDO synthesis modules, as well as pathway engineering to reduce toxic byproducts, the engineered strain demonstrated high production of 1,3-PDO. This study highlights the potential of C. glutamicum as a chassis for the industrial production of 1,3-PDO from lignocellulosic feedstocks.
METABOLIC ENGINEERING
(2022)
Article
Agricultural Engineering
Young Jin Ko, Joy Cha, Wu-Young Jeong, Myeong-Eun Lee, Byeong-Hyeon Cho, Bhardwaj Nisha, Hyun Jin Jeong, Sung Eun Park, Sung Ok Han
Summary: In this study, an engineered Corynebacterium glutamicum was developed to overproduce isopropanol. By identifying key factors and optimizing synthetic pathways, the production of isopropanol was significantly increased. These findings are important for achieving high-level production of isopropanol in C. glutamicum.
BIORESOURCE TECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Rahul Gauttam, Christian K. Desiderato, Dusica Rados, Hannes Link, Gerd M. Seibold, Bernhard J. Eikmanns
Summary: UDP-GlcNAc is a key molecule in biosynthesis and the engineering of Corynebacterium glutamicum has shown great potential as a platform for biosynthesis, demonstrating high levels of UDP-GlcNAc production.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Paul Ramp, Alexander Lehnert, Susana Matamouros, Astrid Wirtz, Meike Baumgart, Michael Bott
Summary: By engineering Corynebacterium glutamicum, competitive strains capable of producing Scyllo-inositol were developed. The use of recombinant strains allowed for the complete conversion of myo-inositol into Scyllo-inositol in glucose-containing medium. Overproduction of key enzymes enabled the production of Scyllo-inositol from cheap carbon sources such as glucose and sucrose.
METABOLIC ENGINEERING
(2021)
Article
Agricultural Engineering
Yinping Xiang, Meiying Jia, Rui Xu, Jialu Xu, Lele He, Haihao Peng, Weimin Sun, Dongbo Wang, Weiping Xiong, Zhaohui Yang
Summary: This study investigated the impact of the non-antibiotic pharmaceutical carbamazepine on antibiotic resistance genes (ARGs) during anaerobic digestion. The results showed that carbamazepine induced the enrichment of ARGs and increased the abundance of bacteria carrying these genes. It also facilitated microbial aggregation and intercellular communication, leading to an increased frequency of ARGs transmission. Moreover, carbamazepine promoted the acquisition of ARGs by pathogens and elevated their overall abundance.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Weixin Zhao, Tianyi Hu, Hao Ma, Dan Li, Qingliang Zhao, Junqiu Jiang, Liangliang Wei
Summary: This review summarizes the effects and potential mechanisms of biochar on microbial behavior in AD systems. The addition of biochar has been found to promote microbial colonization, alleviate stress, provide nutrients, and enhance enzyme activity. Future research directions include targeted design of biochar, in-depth study of microbial mechanisms, and improved models.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Christina Karmann, Anna Magrova, Pavel Jenicek, Jan Bartacek, Vojtech Kouba
Summary: This review assesses nitrogen removal technologies in reject water treatment, highlighting the differences in environmental impacts and economic benefits. Partial nitritation-anammox shows potential for economic benefits and positive environmental outcomes when operated and controlled properly.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Wei-Hao Huang, Ying-Ju Chang, Duu-Jong Lee
Summary: This study modified pinecone biochar with layered double hydroxide (LDH) to enhance its adsorption capacity for heavy metal and phosphate ions. The LDH-biochar showed significantly improved adsorption capacities for Pb2+ and phosphate, and a slight increase for Cu2+ and Co2+. The LDH layer enhanced the adsorption through various mechanisms.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Chao Song, Fanfan Cai, Shuang Yang, Ligong Wang, Guangqing Liu, Chang Chen
Summary: This paper developed a machine learning model to predict the biochemical methane potential during anaerobic digestion. Model analysis identified lignin content, organic loading, and nitrogen content as key attributes for methane production prediction. For feedstocks with high cellulose content, early methane production is lower but can be improved by prolonging digestion time. Moreover, lignin content exceeding a certain value significantly inhibits methane production.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Sang Min Lee, Ju Young Lee, Ji-Sook Hahn, Seung-Ho Baek
Summary: This study successfully developed an efficient platform strain using Yarrowia lipolytica for the bioconversion of renewable resources into adipic acid, achieving a remarkable increase in production level.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Sefkan Kendir, Matthias Franzreb
Summary: This study presents a novel approach using magnetic separation to efficiently harvest freshwater microalgae, Chlorella vulgaris. By combining pH-induced calcium phosphate precipitation with cheap natural magnetite microparticles, harvesting efficiencies up to 98% were achieved in the model medium.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Ishaq Kariim, Ji-Yeon Park, Wajahat Waheed Kazmi, Hulda Swai, In-Gu Lee, Thomas Kivevele
Summary: The impact of reaction temperature, residence time, and ethanol: acetone on the energy compositions and yield enhancement of biocrudes was investigated. The results showed that under appropriate conditions, biocrudes with high energy and low oxygen content can be obtained, indicating a high potential for utilization.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Xiyue Zhang, Xiyao Li, Liang Zhang, Yongzhen Peng
Summary: Intermittent aeration is an innovative approach to enhance nitrogen removal in low carbon-to-nitrogen ratio municipal sewage, providing an efficient strategy for the continuous plug-flow AOA process.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Xu Yang, Mahmoud Mazarji, Mengtong Li, Aohua Li, Ronghua Li, Zengqiang Zhang, Junting Pan
Summary: This study investigated the impact of magnetite on the nitrogen cycle of pig manure biostabilisation. The addition of magnetite increased N2O emissions and decreased NH3 emissions during composting. It also increased the total nitrogen content but should be considered for its significant increase in N2O emissions in engineering practice.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Ty Shitanaka, Haylee Fujioka, Muzammil Khan, Manpreet Kaur, Zhi-Yan Du, Samir Kumar Khanal
Summary: The market value of microalgae has exponentially increased in the past two decades, thanks to their applications in various industries. However, the supply of high-value microalgal bioproducts is limited due to several factors, and strategies are being explored to overcome these limitations and improve microalgae production, thus increasing the availability of algal-derived bioproducts in the market.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Martin Kerner, Thorsten Wolff, Torsten Brinkmann
Summary: The efficiency of using enriched CO2 from flue gas for large-scale production of green microalgae has been studied. The results show that the use of membrane devices and static mixers can effectively improve the CO2 recovery rate and maintain the suitable pH and temperature during cultivation, achieving a more economical and sustainable microalgae production.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Rui Ma, Ji Li, Rd Tyagi, Xiaolei Zhang
Summary: This review summarizes the microorganisms capable of using CO2 and CH4 to produce PHAs, illustrating the production process, factors influencing it, and discussing optimization techniques. It identifies the challenges and future prospects for developing economically viable PHAs production using GHGs as a carbon source.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Bing Wang, Peng Zhang, Xu Guo, Xu Bao, Junjie Tian, Guomin Li, Jian Zhang
Summary: The addition of zeolite in the co-composting of chicken manure and straw significantly reduced the emissions of ammonia and N2O, and increased the nitrate content. Zeolite also promoted the abundance of nitrification genes and inhibited the expression of denitrification genes.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Rohit Dey, Franziska Ortiz Tena, Song Wang, Josef Martin Messmann, Christian Steinweg, Claudia Thomsen, Clemens Posten, Stefan Leu, Matthias S. Ullrich, Laurenz Thomsen
Summary: This study investigated the operation of a 1000L microalgae-based membrane photobioreactor system for continuous secondary wastewater treatment. The research focused on a green microalgae strain called Desmodesmus sp. The study aimed to understand key trends and optimization strategies by conducting experiments in both summer and winter seasons. The findings showed that maintaining low cell concentrations during periods of light inhibition was beneficial for nutrient uptake rates. Effective strategies for enhancing algae-based wastewater treatment included cell mass recycling and adjusting dilution rates based on light availability.
BIORESOURCE TECHNOLOGY
(2024)