Article
Microbiology
Sara Notararigo, Encarnacion Varela, Anna Otal, Ivan Cristobo, Maria Antolin, Francisco Guarner, Alicia Prieto, Paloma Lopez
Summary: Research indicates that the O2-substituted-(1-3)-β-D-glucan synthesized by lactic acid bacteria has prebiotic, immunomodulatory, and anti-inflammatory effects, potentially ameliorating inflammation through modulation of the gut immune system cells.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Sara Notararigo, Encarnacion Varela, Anna Otal, Maria Antolin, Francisco Guarner, Paloma Lopez
Summary: Bacterial beta-glucans are polysaccharides that can protect bacteria or participate in biofilm formation and bacterial cell adhesion. This study found that the O-2-substituted (1-3)-beta-D-glucan synthesized by Pediococcus parvulus 2.6 has a similar structure to laminarin and has the ability to modulate the immune system and adhere to human enterocytes. The study also revealed that this glucan is non-toxic to cells and can regulate cytokine production.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Xinlin Hu, Ping Yang, Changdong Chai, Jia Liu, Huanhuan Sun, Yanan Wu, Mingjie Zhang, Min Zhang, Xiaotian Liu, Hongjun Yu
Summary: This study reveals the structures and mechanisms of yeast FKS1 and the echinocandin-resistant mutant FKS1(S643P) using cryo-electron microscopy, advancing the understanding of fungal β-1,3-glucan biosynthesis and providing insights into drug-resistant mutations of FKS1. It establishes a foundation for the development of new antifungal drugs by targeting FKS.
Article
Nutrition & Dietetics
Wiebke Schloermann, Julia A. Bockwoldt, Sabine M. Huebner, Elisa Wittwer, Sarah Reiners, Stefan Lorkowski, Christine Dawczynski, Matthias A. Ehrmann, Michael Glei
Summary: The aim of this study was to investigate the production of beta-glucan and the potential prebiotic and chemopreventive effects of wheat and rye sourdoughs and breads. The effects of wild-type and non-beta-glucan-forming mutant strains of Levilactobacillus brevis and Pediococcus claussenii were examined. The results showed that concentrations of short-chain fatty acids (SCFA) increased and ammonia concentrations decreased in the fermented samples. Treatment with fermentation supernatants (FS) reduced the growth and viability of LT97 colon adenoma cells and increased caspase-2 and -3 activities. The expression levels of several genes and proteins were also affected.
Article
Chemistry, Applied
Yusuke Matsumoto, Yukiko Enomoto, Satoshi Kimura, Tadahisa Iwata
Summary: The study shows that 1,3-alpha-D-glucan and LMW-1,3-beta-D-glucan hydrogels can be compressed and recover their original shape, while HMW-1,3-beta-D-glucan hydrogels have better deformability and shape recovery.
CARBOHYDRATE POLYMERS
(2021)
Article
Agriculture, Dairy & Animal Science
Rafael Humberto de Carvalho, Marco Aurelio Callegari, Cleandro Pazinato Dias, Susanne Kirwan, Mara Cristina Ribeiro da Costa, Caio Abercio da Silva
Summary: Supplementing the diet of sows with beta G-(1,3) derived from Euglena gracilis algae results in increased colostrum production and consumption, as well as improved weight gain in piglets during the first 18 hours after birth. The colostrum produced by the sows and the blood serum of the piglets also exhibit higher concentrations of immunoglobulins.
Article
Biotechnology & Applied Microbiology
Kiran Kumar Bhukya, Bhima Bhukya
Summary: This study investigated the potential probiotic bacterium Pediococcus pentosaceus OBK05 for its antidiabetic, cholesterol-lowering, and anticancer activities. The results demonstrated significant pharmacological effects of OBK05 both in vitro and in vivo.
PROBIOTICS AND ANTIMICROBIAL PROTEINS
(2022)
Article
Polymer Science
Yuki Hori, Yukiko Enomoto, Satoshi Kimura, Tadahisa Iwata
Summary: Alpha-1,3-glucan and beta-1,3-glucan esters with carbon-carbon double bonds were synthesized from 3-butenoic acid and trifluoroacetic anhydride. Analysis revealed structural transformations and higher molecular weights than the original glucan, suggesting possible intramolecular crosslinking. Surface modifications with thiol compounds via thiol-ene reactions were successful, as confirmed by spectroscopy and contact angle measurements.
POLYMER INTERNATIONAL
(2021)
Article
Chemistry, Medicinal
James M. Apgar, Robert R. Wilkening, Dann L. Parker, Dongfang Meng, Kenneth J. Wildonger, Donald Sperbeck, Mark L. Greenlee, James M. Balkovec, Amy M. Flattery, George K. Abruzzo, Andrew M. Galgoci, Robert A. Giacobbe, Charles J. Gill, Ming-Jo Hsu, Paul Liberator, Andrew S. Misura, Mary Motyl, Jennifer Nielsen Kahn, Maryann Powles, Fred Racine, Jasminka Dragovic, Weiming Fan, Robin Kirwan, Shu Lee, Hao Liu, Ahmed Mamai, Kingsley Nelson, Michael Peel
Summary: By optimizing the chemical structure of MK-5204, researchers discovered ibrexafungerp, which showed significantly improved oral efficacy and is a superior clinical candidate for Aspergillus and Candida infections.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2021)
Article
Biotechnology & Applied Microbiology
Brian W. Vogler, Amy Ashford, Matthew C. Posewitz
Summary: The study knocked out two key enzymes responsible for chrysolaminarin synthesis in Nannochloropsis species using CRISPR/Cas9 technology, resulting in a significant decrease in soluble carbohydrate accumulation following nitrogen starvation.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2021)
Article
Food Science & Technology
Julia A. Bockwoldt, Johanna Fellermeier, Emma Steffens, Rudi F. Vogel, Matthias A. Ehrmann
Summary: Sourdough fermentation is a common practice for improving the quality and shelf life of baked goods globally. Utilizing lactic acid bacteria to form beta-glucan in sourdough offers additional benefits and potential prebiotic properties. Research has shown that methods for bacterial beta-glucan quantification and changes in fermentation temperature can affect beta-glucan yields in sourdoughs, with the sensory analysis indicating some preference among consumers for certain sourdough bread varieties.
Article
Biochemistry & Molecular Biology
Huiwon No, Jimin Kim, Chae-Ryeong Seo, Da Eun Lee, Ji Hyeon Kim, Takao Kuge, Takahiro Mori, Hisashi Kimoto, Jin-Kyung Kim
Summary: The low-molecular-weight A. pullulans-fermented beta-D-glucan showed potential as an immune modulator by reducing the release of inflammatory mediators and modulating key signaling pathways in LPS-stimulated macrophages.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Review
Food Science & Technology
Elena De Marco Castro, Philip C. Calder, Helen M. Roche
Summary: The innate immune system responds rapidly and non-specifically to immunologic threats, while the adaptive immune response is slower but more targeted. Dietary components like yeast-derived beta-glucans may modulate inflammation and antimicrobial activity to aid host defense. Pre-clinical studies suggest that beta-1,3/1,6-glucan derived from baker's yeast could enhance immuno-surveillance, but human evidence is still lacking compared to animal studies.
MOLECULAR NUTRITION & FOOD RESEARCH
(2021)
Article
Microbiology
Young-Ran Song, Chan-Mi Lee, Seon-Hye Lee, Sang-Ho Baik
Summary: PA-M76 demonstrated strong probiotic properties with high tolerance in GIT environments, broad antimicrobial activity, and effective immune response elicitation. Lactic acid fermentation of BRE by PA-M76 resulted in increased antioxidant activity, while not significantly affecting total anthocyanin and flavonoid content.
Article
Infectious Diseases
Laura Barbolini, Arnaud Riat, Christian Van Delden, Jacques Schrenzel
Summary: The inconsistent results of using the biomarker 1,3-beta-D-glucan (BDG) on cerebrospinal fluid (CSF) for diagnosing or following up the treatment of Candida albicans meningitis indicate the need for caution in its application.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Immunology
Lizbeth Estrada-Capetillo, Laura Aragoneses-Fenoll, Angeles Dominguez-Soto, Sara Fuentelsaz-Romero, Concha Nieto, Miriam Simon-Fuentes, Barbara Alonso, Pilar Portoles, Angel L. Corbi, Jose M. Rojo, Amaya Puig-Kroger
Summary: CD28 expression is not limited to T lymphocytes but also exists in macrophages with regulatory functions. Defective CD28 expression in mouse macrophages affects gene expression profile and functionality. Therefore, CD28 expression may serve as a hallmark of anti-inflammatory macrophages and a potential target for immunotherapy.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Sara Notararigo, Encarnacion Varela, Anna Otal, Maria Antolin, Francisco Guarner, Paloma Lopez
Summary: Bacterial beta-glucans are polysaccharides that can protect bacteria or participate in biofilm formation and bacterial cell adhesion. This study found that the O-2-substituted (1-3)-beta-D-glucan synthesized by Pediococcus parvulus 2.6 has a similar structure to laminarin and has the ability to modulate the immune system and adhere to human enterocytes. The study also revealed that this glucan is non-toxic to cells and can regulate cytokine production.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Immunology
Arturo Gonzalez de la Aleja, Cristina Herrero, Monica Torres-Torresano, Juan Vladimir de la Rosa, Barbara Alonso, Enrique Capa-Sardon, Ittai B. Muller, Gerrit Jansen, Amaya Puig-Kroeger, Miguel A. Vega, Antonio Castrillo, Angel L. Corbi
Summary: LXR activation limits the anti-inflammatory polarization of human macrophages and promotes the acquisition of an inflammatory profile, which is associated with the expression of specific genes and the release of activin A.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Food Science & Technology
Maria Laura Werning, Annel M. Hernandez-Alcantara, Maria Julia Ruiz, Lorena Paola Soto, Maria Teresa Duenas, Paloma Lopez, Laureano Sebastian Frizzo
Summary: Lactic acid bacteria (LAB) synthesize exopolysaccharides (EPS), which have diverse technological properties and health-promoting benefits. LAB, especially those producing EPS, are used as probiotics to improve human health and prevent/treat specific diseases. They also play a role in promoting growth and inhibiting pathogens in food-producing animal husbandry. This review discusses the characteristics, usage, biological role, and potential contributions of LAB-produced EPS in improving productivity, animal health, and public health.
Editorial Material
Food Science & Technology
Maria Teresa Duenas, Paloma Lopez
Article
Nutrition & Dietetics
Annel M. Hernandez-Alcantara, Rosana Chiva, Maria Luz Mohedano, Pasquale Russo, Jose Angel Ruiz-Maso, Gloria Del Solar, Giuseppe Spano, Mercedes Tamame, Paloma Lopez
Summary: This work describes a method for obtaining riboflavin overproducing strains of Weissella cibaria through exposure to increasing concentrations of roseoflavin. Mutations in the rib operons of the mutant strains were identified as the molecular basis for riboflavin overproduction. The production of dextran, another product of W. cibaria, was not affected by these mutations. The mutant strains were capable of producing biofortified breads with both riboflavin and dextran during experimental bread making.
FRONTIERS IN NUTRITION
(2022)
Article
Immunology
Alejandra Comins-Boo, Lorena Valdeolivas, Fernando Perez-Pla, Ignacio Cristobal, Nabil Subhi-Issa, Angeles Dominguez-Soto, Lydia Pilar-Suarez, Pilar Gasca-Escorial, Marta Calvo-Urrutia, Miguel Fernandez-Arquero, Miguel Angel Herraiz, Angel Corbi, Silvia Sanchez-Ramon
Summary: This study aimed to evaluate the role of monocyte subsets and cytokines in RPL and RIF women. The study found that CX3CR1+ and CCR5+ intermediate monocytes were significantly higher in RPL and RIF, and there was a significant positive correlation between CX3CR1+ intermediate monocytes and IL-17A. The combination of specific monocyte subsets and cytokines could aid in identifying RPL and RIF women with a pro-inflammatory profile.
JOURNAL OF REPRODUCTIVE IMMUNOLOGY
(2022)
Article
Microbiology
Norhane Besrour-Aouam, Vivian de Los Rios, Annel M. Hernandez-Alcantara, Ma Luz Mohedano, Afef Najjari, Paloma Lopez, Hadda-Imene Ouzari
Summary: Lc. lactis is used by the food industry and is found in vegetables, fruits, and meat. It produces high-molecular-weight dextran from sucrose at low temperatures, and its proteomic profile changes under cold stress. These findings are important for understanding the adaptation of Lc. lactis to low temperature and its potential industrial use.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Arturo Gonzalez de la Aleja, Cristina Herrero, Monica Torres-Torresano, Maria Teresa Schiaffino, Alejandro del Castillo, Barbara Alonso, Miguel A. A. Vega, Amaya Puig-Kroger, Antonio Castrillo, Angel L. Corbi
Summary: Monocyte-derived macrophages play a role in inflammatory diseases and their functions are influenced by the extracellular environment. Liver X Receptors (LXRs) control cholesterol metabolism and macrophage differentiation. Inhibition of LXRs can promote the anti-inflammatory gene and functional profile of human macrophages in a MAFB-dependent manner.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Article
Microbiology
Inaki Diez-Ozaeta, Lucia Martin-Loarte, Mari Luz Mohedano, Mercedes Tamame, Jose Angel Ruiz-Maso, Gloria del Solar, Maria Teresa Duenas, Paloma Lopez
Summary: This study developed a new method to select riboflavin-overproducing strains of Weissella cibaria using resistance to roseoflavin as a marker. DNA sequencing revealed mutations in the FMN riboswitch, potentially deregulating riboflavin synthesis. The selected mutants showed promising riboflavin production and potential applications in the food and health industry.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Food Science & Technology
Ana Isabel Puertas, Ma Goretti Llamas-Arriba, Oier Etxebeste, Inaki Berregi, Miguel Angel Pardo, Alicia Prieto, Paloma Lopez, Teresa Duenas
Summary: This study characterizes the physico-chemical properties of heteropolysaccharides (HePS) produced by Liquorilactobacillus sicerae CUPV261T and Secundilactobacillus collinoides CUPV237 strains isolated from cider. Genome sequencing revealed the presence of at least four putative HePS gene clusters in each strain, which correlated with their ability to secrete HePS. The HePS were resistant to gastric stress conditions and showed significant reduction in zebrafish larvae mortality in an in vivo model of inflammatory bowel disease.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Alba Garrido-Trigo, Ana M. Corraliza, Marisol Veny, Isabella Dotti, Elisa Melon-Ardanaz, Aina Rill, Helena L. Crowell, Angel Corbi, Victoria Gudino, Miriam Esteller, Iris alvarez-Teubel, Daniel Aguilar, M. Carme Masamunt, Emily Killingbeck, Youngmi Kim, Michael Leon, Sudha Visvanathan, Domenica Marchese, Ginevra Caratu, Albert Martin-Cardona, Maria Esteve, Julian Panes, Elena Ricart, Elisabetta Mereu, Holger Heyn, Azucena Salas
Summary: Ulcerative colitis and Crohn's disease are chronic inflammatory intestinal diseases with perplexing heterogeneity in disease manifestation and response to treatment. Single-cell technologies are applied to explore the transcriptional states within tissues and spatially localize macrophages and neutrophils subsets, revealing further macrophage diversity based on tissue localization and communication network involving macrophages and inflammatory fibroblasts. The cellular complexity of these diseases and myeloid and stromal compartments are important for understanding patient-to-patient heterogeneity.
NATURE COMMUNICATIONS
(2023)
Article
Immunology
Miriam Simon-Fuentes, Cristina Herrero, Lucia Acero-Riaguas, Concha Nieto, Fatima Lasala, Nuria Labiod, Joanna Luczkowiak, Barbara Alonso, Rafael Delgado, Maria Colmenares, Angel L. Corbi, Angeles Dominguez-Soto
Summary: This study investigates the expression and function of Toll-like receptor 7 (TLR7) in monocyte-derived macrophages. TLR7 activation leads to the production of type I interferon and reprograms the gene transcription profile of these cells, inducing the expression of neutrophil-attracting chemokines. The results suggest that aberrant TLR7 signaling is associated with delayed resolution of inflammation following viral infection.
JOURNAL OF INNATE IMMUNITY
(2023)
