Article
Microbiology
Yixin Zhang, Wei Wei, Huamei Wen, Zhongle Cheng, Zhongwen Mi, Jing Zhang, Xiaolong Liu, Xinjiong Fan
Summary: This study introduces a novel biofilm-targeted strategy using combined enzymes to prevent biofilm formation and break down preformed biofilms. By modifying the quorum-sensing inhibitor AidH and combining it with the glycosyl hydrolase PslG, the combined-enzyme intervention effectively disrupts biofilms of Pseudomonas aeruginosa. The strategy also helps reverse antibiotic resistance in multidrug-resistant clinical strains. This research highlights the potential clinical application of the combined-enzyme approach for P. aeruginosa infection.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Infectious Diseases
Viviane C. Oliveira, Ana P. Macedo, Luis D. R. Melo, Silvio B. Santos, Paula R. S. Hermann, Claudia H. Silva-Lovato, Helena F. O. Paranhos, Denise Andrade, Evandro Watanabe
Summary: This study evaluated the effectiveness of five bacteriophages in removing and preventing Pseudomonas aeruginosa biofilms on endotracheal tubes, finding a significant reduction in biofilms, especially in multidrug-resistant strains, after phage treatment. Two phages were selected as cocktail components, showing potential in preventing bacterial colonization on tube surfaces in a dynamic biofilm model.
Article
Chemistry, Multidisciplinary
Pengyu Li, Jieyi Pan, Yating Dong, Yingying Sun, Yalong Wang, Kang Liao, Yili Chen, Xin Deng, Shihui Yu, Haiyan Hu
Summary: Chronic pulmonary infection caused by Pseudomonas aeruginosa is a serious public health problem with high mortality rates. In this study, infection-microenvironment responsive nanoparticles were developed to eradicate biofilms and inhibit virulence. These nanoparticles showed promising results in treating chronic pulmonary infections.
JOURNAL OF CONTROLLED RELEASE
(2024)
Review
Pharmacology & Pharmacy
Yapeng Zhang, Xuanhe Pan, Linqian Wang, Liyu Chen
Summary: Pseudomonas aeruginosa, a gram-negative bacterium, is able to form biofilm on various surfaces, enhancing its antibiotic resistance and adaptability. Iron metabolism plays a crucial role in biofilm development of P. aeruginosa, offering potential targets for novel anti-biofilm therapeutics.
JOURNAL OF DRUG TARGETING
(2021)
Article
Microbiology
Rakesh Sikdar, Mikael H. Elias
Summary: Quorum sensing (QS) is a cell-to-cell communication system that regulates bacterial behaviors. Enzymatic quorum quenching (QQ) can interfere with QS and potentially enhance the effects of antibiotics. This study evaluated the impact of QS signal disruption on antibiotic resistance in Pseudomonas aeruginosa and found that it significantly affects resistance. The results highlight the complex relationship between QS and antibiotic resistance and suggest a case-by-case approach to combination therapy.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Mizuki Kanno, Takuya Shiota, So Ueno, Minato Takahara, Keisuke Haneda, Yuhei O. Tahara, Masaki Shintani, Ryoma Nakao, Makoto Miyata, Kazuhide Kimbara, Hiroyuki Futamata, Yosuke Tashiro
Summary: Membrane vesicles (MVs) are small spherical structures produced by bacteria, playing important roles in bacterial biology and host immune modulation. This study revealed enhanced MV formation in Pseudomonas aeruginosa biofilms, which is closely related to biofilm matrix synthesis and surface sensing.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Esther Shmidov, Ilana Lebenthal-Loinger, Shira Roth, Sarit Karako-Lampert, Itzhak Zander, Sivan Shoshani, Amos Danielli, Ehud Banin
Summary: The functions of bacterial TA systems are still disputed and poorly understood. This study sheds light on potential functions of bacterial TA systems. The novel type II TA system PrrT/A in Pseudomonas aeruginosa is found to regulate biofilm formation, motility, prophage induction, bacteriophage production, and aminoglycoside resistance, providing new insights into the diverse roles and impacts of TA systems in bacteria.
MICROBIOLOGY SPECTRUM
(2022)
Article
Engineering, Environmental
Han-Shin Kim, Yongsun Jang, So-Young Ham, Jeong-Hoon Park, Hyun-Jin Kang, Eun Tae Yun, Dong-Hyup Shin, Kyoung-Chul Kim, Hee-Deung Park
Summary: The study evaluated the biofouling inhibition capacity of raffinose during the MBR process, showing that raffinose significantly reduced the formation of the P. aeruginosa and S. aureus co-culture biofilm in a concentration-dependent manner. Raffinose was found to be effective in inhibiting biofilm formation in mixed cultures, demonstrating its potential for mitigating biofouling in MBR processes.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Infectious Diseases
Taehyeong Lim, So-Young Ham, SangJin Nam, Myoungsun Kim, Ki Yong Lee, Hee-Deung Park, Youngjoo Byun
Summary: This article reviews recent studies on small molecules targeting the RhlI/RhlR system in P. aeruginosa, discussing their in vitro and in vivo biological activities. The article focuses on structure-activity relationship studies and the outlook for next-generation anti-biofilm agents.
Article
Microbiology
Magdalena Ratajczak, Dorota Kaminska, Jolanta Dlugaszewska, Marzena Gajecka
Summary: The study assessed the antibiotic resistance and biofilm formation of bacteria in pharmaceutical production sites, finding that the majority of P. aeruginosa strains exhibited resistance to antibiotics and strong biofilm-forming ability, as well as containing genes encoding various virulence factors.
Article
Biotechnology & Applied Microbiology
James Redfern, Janine Wallace, Alex van Belkum, Magali Jaillard, Elliot Whittard, Roobinidevi Ragupathy, Joanna Verran, Peter Kelly, Mark Charles Enright
Summary: Pseudomonas aeruginosa is a common environmental microorganism that can form biofilms on indwelling device surfaces, promoting adhesion and survival. Research identified accessory genes and core-genome SNPs associated with enhanced biofilm production at 22 degrees C, including a 165 kb genomic island with heavy metal resistance genes.
Review
Immunology
Aref Shariati, Zahra Chegini, Ehsanollah Ghaznavi-Rad, Ehsan Nazarzadeh Zare, Seyed Mostafa Hosseini
Summary: The biofilm community is the dominant mode of microbial growth in nature and contributes to the prevalence of multidrug-resistant strains. Poly lactic-co-glycolic acid (PLGA)-based nanoplatforms show promise in delivering anti-biofilm agents (ABAs) for the destruction of microbial biofilms. However, further research is needed to evaluate the exact interaction between PLGA and biofilms.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Environmental Sciences
Kathirvel Brindhadevi, Quynh Hoang Le, Saleh H. Salmen, Indira Karuppusamy, Arivalagan Pugazhendhi
Summary: Biofilms consist of Gram-positive and Gram-negative bacteria enclosed in a matrix. Industrial biofouling caused by biofilms is a concern due to the antimicrobial resistance exhibited by these biofilms. This study evaluates the antibacterial and antibiofilm activity of Aerva lanata flower extract against Staphylococcus aureus and Pseudomonas aeruginosa, demonstrating its potential as a therapeutic in combating pathogenic bacterial biofilms.
ENVIRONMENTAL RESEARCH
(2023)
Article
Multidisciplinary Sciences
Arpit Shukla, Gaurav Shukla, Paritosh Parmar, Baldev Patel, Dweipayan Goswami, Meenu Saraf
Summary: The constant threat of multidrug resistance in human pathogens, led by Pseudomonas aeruginosa, is a significant challenge to human health. The phenomenon of quorum sensing (QS) plays a crucial role in the pathogenicity of P. aeruginosa, and natural compounds like gingerol and curcumin have been identified as QS antagonists that can inhibit virulence and biofilm formation in this bacterium.
SCIENTIFIC REPORTS
(2021)
Article
Infectious Diseases
Tomasz Bogiel, Dagmara Depka, Mateusz Rzepka, Joanna Kwiecinska-Pirog, Eugenia Gospodarek-Komkowska
Summary: The study investigated the frequency and genotype distribution of 10 virulence factor genes in 107 non-duplicated carbapenem-resistant P. aeruginosa isolates. Variations were observed in the prevalence and distribution of virulence genes, especially in terms of their carbapenemase synthesis ability and the strain origin.
Article
Biochemistry & Molecular Biology
Andrea J. Weiler, Olivia Spitz, Mirja Gudzuhn, Stephan N. Schott-Verdugo, Michael Kamel, Wolfgang R. Streit, Alexej Kedrov, Lutz Schmitt, Holger Gohlke, Filip Kovacic
Summary: This study reveals the role of a novel phospholipase B, PaPlaB, in Pseudomonas aeruginosa. PaPlaB is involved in biofilm formation and cell localization, and may serve as a potential therapeutic target.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2022)
Article
Biochemistry & Molecular Biology
Pascal Rath, Johanna Rapp, Klaus Brilisauer, Marvin Braun, Uener Kolukisaoglu, Karl Forchhammer, Stephanie Grond
Summary: This study establishes a powerful hybrid synthetic approach for efficiently obtaining various C-7 sugar analogues and applies them for structure-activity relationship studies. It reveals that 7dSh could serve as a valid target for herbicides and antimicrobial agents.
Article
Biochemistry & Molecular Biology
Jochen Sieg, Chris Claudius Sandmeier, Julia Lieske, Alke Meents, Christian Lemmen, Wolfgang R. Streit, Matthias Rarey
Summary: Protein adaptations to extreme environments, such as high temperature and high pressure in the deep sea, are important for biotechnological process optimization and understanding the molecular limits of life. This study evaluates the differences in protein structures between deep-sea organisms and non-deep-sea organisms, aiming to uncover the molecular mechanisms of adaptations to high pressure. The results show characteristic differences in sequence and structure, indicating the presence of unique correlations and potential adaptations to high pressure.
PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS
(2022)
Review
Biotechnology & Applied Microbiology
Ines Krohn, Simon Menanteau-Ledouble, Gunhild Hageskal, Yekaterina Astafyeva, Pierre Jouannais, Jeppe Lund Nielsen, Massimo Pizzol, Alexander Wentzel, Wolfgang R. Streit
Summary: This article provides an overview of the health benefits of microalgae and their associated microbiota, highlighting their potential as a source of antimicrobial and prebiotic substances. It suggests that microalgae could be a solution to the shortage of antibiotics for treating antimicrobial resistant bacteria and fungi in clinical settings.
MICROBIAL BIOTECHNOLOGY
(2022)
Article
Microbiology
Mirja Gudzuhn, Ifey Alio, Raphael Moll, Jessica de Vries, Jacob Boehlich, Maik Assmann, Jasmin Janneschuetz, Nina Schuetzenmeister, Axel Himmelbach, Anja Poehlein, Rolf Daniel, Wolfgang R. Streit
Summary: Stenotrophomonas maltophilia is a multidrug-resistant human opportunistic pathogen that causes progression of disease in cystic fibrosis patients and is commonly found in wounds, infected tissues, and catheter surfaces. Due to its well-known multidrug resistance, treating infections caused by S. maltophilia is difficult. Recent studies have shown that certain fungal diorcinols and rubrolides can reduce biofilm formation of S. maltophilia. In this study, a larger number of diorcinols and rubrolides were tested and the molecular mechanisms behind their antibiofilm effects were investigated. The compounds tested significantly reduced biofilm thickness and had strong effects on mature biofilms. Genes involved in iron transport, general metabolism, and membrane biosynthesis were found to be most strongly regulated by these compounds. The presence of prenyl group in diorcinols and increasing degree of bromination in chlorinated rubrolides were possibly responsible for their strong antibiofilm effects. This study provides valuable insights into the effects of rubrolides and diorcinols on biofilms formed by the important pathogen S. maltophilia.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Yekaterina Astafyeva, Marno Gurschke, Minyue Qi, Lutgardis Bergmann, Daniela Indenbirken, Imke de Grahl, Elena Katzowitsch, Sigrun Reumann, Dieter Hanelt, Malik Alawi, Wolfgang R. Streit, Ines Krohn
Summary: This study provides a detailed insight into the mutualistic collaboration between microalgae and bacteria, including the competitive interplay among bacteria. The experimental evidence suggests that Gram-negative bacteria of the Dyadobacter, Porphyrobacter, and Variovorax genera play key roles in the interaction between Scenedesmus quadricauda and bacteria. It is shown that Dyadobacter produces and releases polysaccharide degradation enzymes and leucine-rich repeat proteins; Variovorax supplies the consortium with auxins and vitamin B12, while Porphyrobacter produces a wide range of B vitamins. The study not only demonstrates the collaboration between microalgae and bacteria, but also the interaction among bacteria through quorum-sensing and secretion system mechanisms. The shared currency between partners appears to be vitamins, microalgae growth-promoting substances, and dissolved carbon.
MICROBIOLOGY SPECTRUM
(2022)
Article
Multidisciplinary Sciences
Yuchen Han, Birhanu M. Kinfu, Fabian Blombach, Gwenny Cackett, Hongli Zhang, Pablo Perez-Garcia, Ines Krohn, Jesper Salomon, Volkan Besirlioglu, Tayebeh Mirzaeigarakani, Ulrich Schwaneberg, Jennifer Chow, Finn Werner, Wolfgang R. Streit
Summary: Metagenomics is a powerful tool to discover novel proteins and other valuable biomolecules. However, function-based metagenome searches are often limited by the time-consuming expression of active proteins. In this study, researchers identified a novel single-subunit bacteriophage RNA polymerase, EM1 RNAP, from a metagenome data set obtained from an elephant dung microbiome. They developed an efficient medium-throughput pipeline and protocol using EM1 RNAP and a translation-competent Escherichia coli extract to express metagenome-derived genes and produce proteins in a cell-free system, allowing for initial testing of predicted activities.
SCIENTIFIC REPORTS
(2022)
Article
Microbiology
Yekaterina Astafyeva, Marno Gurschke, Wolfgang R. Streit, Ines Krohn
Summary: Based on previous research, an artificial plant-bacteria system was established to study the mutualistic collaboration between a microalga and its microbiome. The study focused on the mechanism of a type IX secretion system (T9SS) and its role in the attachment and invasion of the microalga by a bacterial isolate. Genome analysis revealed the presence of T9SS genes and other genes associated with gliding motility and protein secretion. The study proposed a model for the T9SS apparatus of the bacterial isolate and examined the bacterial colonization and penetration into the cell wall of the microalga.
FRONTIERS IN MICROBIOLOGY
(2022)
Review
Biotechnology & Applied Microbiology
Jennifer Chow, Pablo Perez-Garcia, Robert Dierkes, Wolfgang R. Streit
Summary: Global economies heavily rely on fossil-fuel-based polymers, but a significant amount of these synthetic polymers are disposed into the environment. Over the past decade, microbiologists have been searching for enzymes that can degrade plastic, but there have been limited success in finding functional biocatalysts for most plastics. The main challenge now is to identify enzymes that can target the majority of fossil-fuel-based plastics. This emerging research field is still in its infancy.
MICROBIAL BIOTECHNOLOGY
(2023)
Article
Biology
Charlotte Marie Ahle, Kristian Stodkilde, Anja Poehlein, Mechthild Boemeke, Wolfgang R. Streit, Horst Wenck, Joern Hendrik Reuter, Jennifer Huepeden, Holger Brueggemann
Summary: Research has found that the human skin is inhabited by a large number of microbes, collectively known as the skin microbiome. Among them, Staphylococcus epidermidis and Cutibacterium acnes are the most common members. The study reveals that Staphylococcus epidermidis can selectively exclude acne-associated bacteria and coexist with healthy skin-associated bacteria, thus contributing to the maintenance of healthy skin homeostasis through regulation of antimicrobial activity.
COMMUNICATIONS BIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Robert F. Dierkes, Alan Wypych, Pablo Perez-Garcia, Dominik Danso, Jennifer Chow, Wolfgang R. Streit
Summary: A fluorescent biosensor based on Comamonas thiooxidans strain S23 was constructed in this study. This biosensor has high sensitivity and can detect the PET breakdown product TPA at concentrations as low as 10 μM. Therefore, it can be used for the detection of enzymatic PET breakdown products.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Sebastian Weigert, Pablo Perez-Garcia, Florian J. Gisdon, Andreas Gagsteiger, Kristine Schweinshaut, G. Matthias Ullmann, Jennifer Chow, Wolfgang R. Streit, Birte Hoecker
Summary: The handling of plastic waste and microplastic has become one of the greatest challenges of our time. Recent studies on plastic degrading enzymes have provided new prospects for biological decomposition and recycling. In this study, the candidate PET6 enzyme from Vibrio gazogenes was chosen for further investigation. The characterization of PET6 revealed its widespread occurrence in various Vibrio strains and its superior ability to degrade polyethylene terephthalate under high salt concentrations. Mutations from other PET degrading enzymes were introduced to PET6 to enhance its activity, demonstrating the evolutionary potential of the enzyme. The crystal structure and molecular dynamics simulations of PET6 provided insights into its adaptation to saline environments. The findings suggest that Vibrio and the PET6 enzyme are worthy subjects for studying PET degradation in marine environments.
Article
Biotechnology & Applied Microbiology
Uwe Mamat, Manuel Hein, Doerte Grella, Claire S. Taylor, Thomas Scholzen, Ifey Alio, Wolfgang R. Streit, Pol Huedo, Xavier Coves, Oscar Conchillo-Sole, Andromeda-Celeste Gomez, Isidre Gibert, Daniel Yero, Ulrich E. Schaible
Summary: Using a Tn7-based genomic integration system, improved mini-Tn7 delivery plasmids were constructed to label S. maltophilia with sfGFP, mCherry, tdTomato, and mKate2. The transposition of mini-Tn7 transposons had no adverse effects on the fitness of fluorescently labeled S. maltophilia derivatives. The mini-Tn7 elements remained stably integrated in the genome of S. maltophilia over a prolonged period of time in the absence of antibiotic selection pressure.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Review
Polymer Science
Leon Klose, Neele Meyer-Heydecke, Sasipa Wongwattanarat, Jennifer Chow, Pablo Perez Garcia, Camille Carre, Wolfgang Streit, Garabed Antranikian, Ana Malvis Romero, Andreas Liese
Summary: Epoxy resins are highly valued for their properties and applications, but the challenge lies in their non-biodegradability and unsustainable recycling methods. Recent progress has been made in plastic biodegradation, but mainly focuses on other polymers, leaving epoxy underrepresented. This review examines the approaches and challenges in biodegradation and recycling of epoxy, and highlights the analytical techniques used.
Article
Biochemistry & Molecular Biology
Mandy Dittmer, Florian H. H. Brill, Andreas Kampe, Maria Geffken, Julian-Dario Rembe, Raphael Moll, Ifey Alio, Wolfgang R. Streit, Eike Sebastian Debus, Ralf Smeets, Ewa Klara Stuermer
Summary: Biofilms in hard-to-heal wounds are a significant issue, often composed of different species and resistant to antimicrobial agents. This study quantitatively analyzed the tolerance of dual-species wound biofilm models to antimicrobials and antimicrobial dressings, finding that the efficacy and tolerance were nearly species-independent.