Article
Cell Biology
Daniel Simoes de Jesus, Paula Bargi-Souza, Vinicius Cruzat, Vijay Yechoor, Angelo Rafael Carpinelli, Rodrigo Antonio Peliciari-Garcia
Summary: The pancreatic beta cells circadian clock and NADPH oxidase (NOX) family play important roles in glucose metabolism. Absence of BMAL1 may trigger an increase in NOX2-derived reactive oxygen species (ROS) in pancreatic beta cells, leading to inhibition of insulin secretion.
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Anthony L. Sylvester, David X. Zhang, Sophia Ran, Natalya S. Zinkevich
Summary: Reactive oxygen species (ROS) play important roles in health and disease, with both beneficial and harmful effects. Different sources of ROS have different roles in various pathologies. NOX1 is associated with hypertension through ROS production, making it a promising therapeutic target. NOX2 and NOX4 produce hydrogen peroxide, influencing cellular processes. Increased ROS production from NOX5 contributes to atherosclerosis.
Article
Toxicology
Masahide Inoue, Koji Sakamoto, Atsushi Suzuki, Shinya Nakai, Akira Ando, Yukihiko Shiraki, Yoshio Nakahara, Mika Omura, Atsushi Enomoto, Ikuhiko Nakase, Makoto Sawada, Naozumi Hashimoto
Summary: Our study using a murine model demonstrated that the pulmonary toxicity of silica nanoparticles depended on their physico-chemical properties through distinct mechanisms. Cellular uptake of larger particles by macrophages decreased, while surface amine modification modulated endosomal reactive oxygen species (ROS) signaling via NADPH oxidase 2 (NOX2), both of which were involved in mitigating the immune response in macrophages and resulting lung injury.
PARTICLE AND FIBRE TOXICOLOGY
(2021)
Article
Plant Sciences
Arkajo Majumdar, Rup Kumar Kar
Summary: Plasma membrane located NADPH oxidase (NOX) and H+-ATPase function together to maintain membrane electrical balance and mediate root growth through wall relaxation. The transcriptional co-regulation of these two enzymes, mediated by H2O2 and Ca+ 2, is essential for root growth. The involvement of polyamine metabolism in ROS-mediated root growth regulation has also been demonstrated. Inhibition of polyamine oxidase leads to reduced NOX and H+-ATPase activities, while exogenous application of polyamines stimulates their cascade. The interplay between NOX-PM H+-ATPase cascade and polyamine metabolism is mediated by H2O2 and Ca+ 2 during early root growth in Vigna radiata (L.) Wilczek seedlings.
JOURNAL OF PLANT GROWTH REGULATION
(2023)
Article
Biochemistry & Molecular Biology
Tian Xie, Zehua Duan, Shilong Sun, Chengnan Chu, Weiwei Ding
Summary: The study found that beta-Lactams exert immunomodulation effects through modulating neutrophil extracellular traps (NETs) formation, depending on the PKC-Akt-mTOR signal pathway.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Biotechnology & Applied Microbiology
Fuju Tai, Shuai Wang, Benshuai Liang, Yue Li, Jiakai Wu, Chenjie Fan, Xiuli Hu, Hezhong Wang, Rui He, Wei Wang
Summary: This study discovered the positive effect of fullerene-based carbon nanomaterials on maize root-hair growth under induced oxidative stress. The application of IFQA can ameliorate oxidative stress by decreasing NADPH oxidase activity, improving ROS scavenging through the ASA-GSH cycle, and regulating the expressions of genes affecting maize-root-hair development. This research enriches the understanding of the mechanism by which fullerene-based nanoelicitors promote plant growth and protect against oxidative stress.
JOURNAL OF NANOBIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Mara Fiorani, Rita De Matteis, Barbara Canonico, Giulia Blandino, Alessandro Mazzoli, Mariele Montanari, Andrea Guidarelli, Orazio Cantoni
Summary: The conversion of human SW872 preadipocytes to mature adipocytes involves time-dependent changes in differentiation markers' expression, morphological changes, and accumulation of lipid droplets. Reactive oxygen species (ROS) formation was significant at specific time points during differentiation, with NADPH oxidase (NOX)-2 playing a role. Mitochondrial ROS (mROS) were only detected in the late phase of differentiation, indicating potential mitochondrial dysfunction. These findings suggest a potential link between ROS, adipogenesis, and adipose tissue dysfunction in SW872 cells.
Review
Biochemistry & Molecular Biology
Tiziana Pecchillo Cimmino, Rosario Ammendola, Fabio Cattaneo, Gabriella Esposito
Summary: Reactive oxygen species (ROS) are highly reactive molecules that can induce cytotoxicity or regulate cellular physiology. Among the ROS, the superoxide anion radical (O2 center dot-) plays a key role in redox signaling, generated by the NADPH oxidase (NOX) enzyme family and the mitochondrial electron transport chain. In this review, the authors discuss the emerging roles of NOX enzymes in modulating cancer cell metabolism and highlight their potential as therapeutic targets for rewiring tumor metabolism.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Stephenson B. Owusu, Elodie Hudik, Celine Ferard, Sophie Dupre-Crochet, Eric C. D. K. Addison, Kwasi Preko, Tania Bizouarn, Chantal Houee-Levin, Laura Baciou
Summary: Simulating oxidative stress with gamma irradiation leads to shortened half-lives of neutrophils but pre-activates surviving cells to produce superoxide anions. Incomplete assembly of the NADPH oxidase complex after irradiation significantly enhances neutrophil reactivity.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Rana M. Nassif, Elias Chalhoub, Pia Chedid, Margarita Hurtado-Nedelec, Elia Raya, Pham My-Chan Dang, Jean-Claude Marie, Jamel El-Benna
Summary: Metformin exhibits anti-inflammatory effects by inhibiting the differentiation of human monocytes into proinflammatory macrophages and by limiting ROS production by macrophages via the activation of AMPK.
Article
Microbiology
Hasnaa Maksouri, Dounia Darif, Jerome Estaquier, Myriam Riyad, Christophe Desterke, Meryem Lemrani, Pham My-Chan Dang, Khadija Akarid
Summary: This study evaluated the impact of Moroccan strains of L. major and L. tropica on PMNs. The results showed that L. tropica inhibited O-2(-) production, while L. major did not. Additionally, Leishmania soluble antigens from both species inhibited O-2(-) induction.
Article
Agronomy
Jie Li, Lidan Feng, Dong Li, Xianglin Liu, Yangyang Pan, Jing He, Junxia Zhang
Summary: This study investigates the role of ROS mediated by NADPH oxidase in the growth of F. solani. The results demonstrate that NCF2 gene plays a significant role in regulating the activity of NADPH oxidase and the expression of ROS metabolism enzyme genes, which affects colony growth in F. solani. Furthermore, exogenous H2O2 treatment also influences the growth and ROS metabolism of F. solani.
Article
Oncology
Hui Chen, Han Zhang, Lixing Cao, Jinling Cui, Xuan Ma, Chong Zhao, Shutao Yin, Hongbo Hu
Summary: Sodium selenite has a stronger lethality to cancer cells under glucose limitation and an enhanced inhibitory effect on tumor growth when combined with intermittent fasting. Additionally, this treatment has no obvious toxicity to normal cells and mice.
Article
Biotechnology & Applied Microbiology
Mengxue Liu, Yao Liu, Lei Zhang, Facheng Qiu
Summary: In this study, the function of NADPH oxidase in Chlorella pyrenoidosa was investigated. The results showed that NADPH oxidase actively participated in the production of intracellular ROS, and the ROS levels were higher under nitrogen and phosphorus-deficient conditions.
BIOTECHNOLOGY LETTERS
(2023)
Article
Biochemistry & Molecular Biology
Giulia Blandino, Mara Fiorani, Barbara Canonico, Rita De Matteis, Andrea Guidarelli, Mariele Montanari, Gloria Buffi, Lucia Coppo, Elias S. J. Arner, Orazio Cantoni
Summary: Long-term treatment of schizophrenia with clozapine may lead to metabolic disorders, and this study found that clozapine can inhibit adipogenesis and reduce reactive oxygen species produced by NADPH oxidase. Clozapine also suppresses antioxidant responses and promotes mitochondrial superoxide formation, which may be associated with insulin resistance and metabolic syndrome. These cellular events may provide insights into the molecular mechanisms underlying the adverse metabolic effects of clozapine.