Article
Biochemistry & Molecular Biology
Yu-Ran Lee, Eun Young Bae, Hong Ryang Kil, Byeong-Hwa Jeon, Geena Kim
Summary: This study investigated the association between APE1/Ref-1 and Kawasaki disease (KD). The results showed that APE1/Ref-1 levels were significantly higher in KD patients, especially in refractory KD. APE1/Ref-1 could be a beneficial biomarker for the diagnosis and prognosis of KD.
Article
Medicine, General & Internal
In Seol Yoo, Yu-Ran Lee, Seong Wook Kang, Jinhyun Kim, Hee-Kyoung Joo, Su-Jin Yoo, Chan Keol Park, Ha-Reum Lee, Ji Ah Park, Byeong-Hwa Jeon
Summary: The study demonstrates that the levels of APE1/Ref-1 are significantly elevated in the serum and synovial fluid of rheumatoid arthritis patients, and are positively correlated with disease activity.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Huanhuan Wang, Linghao Zhang, Huaqing Sun, Shufeng Xu, Kun Li, Xin Su
Summary: In this study, we developed an inhibitory aptamer for APE1 using SELEX technology. The aptamer showed high binding affinity and could entirely inhibit the activity of APE1. These aptamers can be utilized for early cancer diagnosis and treatment, as well as a valuable tool for studying the function of APE1.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Chemistry, Analytical
Yuqiang Hu, Zhen Zhang, Weicong Ye, Wei Zhang, Minghao Hu, Wenqian Yuan, Hongbo Wang, Xianjin Xiao, Tongbo Wu
Summary: Human apurinic/apyrimidinic endonuclease 1 (APE1) is a key player in DNA repair and gene regulation, with abnormal variations of its concentration in human blood and tissue cells being highly correlated to various diseases. This study introduces a DNA structure-mediated fluorescent biosensor for detecting APE1 activity with high sensitivity and selectivity. Through clever design, the biosensor improves APE1 reactivity and minimizes interference from other enzymes, achieving a low detection limit of 1.7 x 10(-6) U/mL and successful application in real biological samples and screening of APE1 inhibitors.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Cell Biology
Thais Teixeira Oliveira, Fabricia Lima Fontes-Dantas, Rayssa Karla de Medeiros Oliveira, Daniele Maria Lopes Pinheiro, Leonam Gomes Coutinho, Vandeclecio Lira da Silva, Sandro Jose de Souza, Lucymara Fassarella Agnez-Lima
Summary: Chemical inhibition of APE1/Ref-1 affects gene expression in an inflammatory cellular model, reducing the expression of certain cytokines and chemokines. It also impacts transcriptional factors such as NRF1 and ETS family regulators, suggesting partial overlap between APE1 redox and DNA repair functions.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Gabriella D. Hartman, Nathan A. Lambert-Cheatham, Mark R. Kelley, Timothy W. Corson
Summary: As global population ages, the prevalence of neovascular eye diseases is increasing, highlighting the importance of developing therapies targeting multiple pathways to alleviate the burden and risks associated with current treatments.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Lauren Sahakian, Ainsley M. Robinson, Linda Sahakian, Rhian Stavely, Mark R. Kelley, Kulmira Nurgali
Summary: Inflammatory bowel disease (IBD) is a chronic relapsing inflammation of the gastrointestinal tract. The prevalence of IBD is increasing worldwide, calling for the development of new treatments. APE1/Ref-1 has been identified as a potential target for IBD therapy due to its role in regulating crucial pathways in inflammatory diseases. This review discusses the current status of IBD treatments, the role of APE1/Ref-1 in intestinal inflammation, and the potential of small molecule inhibitors to modulate inflammation and oxidative stress in IBD.
Article
Biochemistry & Molecular Biology
Arianna Barchiesi, Veronica Bazzani, Agata Jabczynska, Lukasz S. Borowski, Silke Oeljeklaus, Bettina Warscheid, Agnieszka Chacinska, Roman J. Szczesny, Carlo Vascotto
Summary: APE1 is a versatile protein involved in DNA repair, gene expression regulation, and RNA metabolism in mitochondria. Loss of APE1 leads to accumulation of damaged mitochondrial mRNA, impairing protein translation and mitochondrial respiration efficiency. The data demonstrate that APE1 plays a crucial role in maintaining mitochondrial well-being.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Suravi Pramanik, Yingling Chen, Heyu Song, Irine Khutsishvili, Luis A. Marky, Sutapa Ray, Amarnath Natarajan, Pankaj K. Singh, Kishor K. Bhakat
Summary: This study identifies a novel role for the protein APE1 in regulating stable G4 formation and KRAS expression in PDAC, suggesting that G4 structures could be potential prognostic markers and therapeutic targets for PDAC.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Chemistry, Analytical
Yanting Feng, Zewei Luo, Juan Wu, Zhijun Huang, Lu He, Yaqiang Feng, Xian Li, Xudong Zhang, Yonghui Tian, Yongxin Li, Yixiang Duan
Summary: A new DNA logic platform coupled with multivariate statistical analysis was developed to classify seven different types of cancer cells. The platform demonstrated high specificity and sensitivity, generating strong dual fluorescent output signals in living cells.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Medicine, Research & Experimental
Zhou Hai, Qin Jia
Summary: The multifunctional key protein APE1 is closely associated with asthma, and the mRNA levels of APE1 in peripheral blood neutrophils can be used as a marker for diagnosing and distinguishing the disease.
INVESTIGACION CLINICA
(2022)
Article
Biochemistry & Molecular Biology
Junyu Fan, Manqi Liu, Xiaomin Li, ShengLan Gao, Yahong Wang, Ao Li, Lujun Chen, Dengshuang Zhou, Hongqiao Chen, Zhiliang Xu, Zijun Wu, Keng Wu
Summary: Cardiomyocyte apoptosis caused by fat metabolism disorder plays an essential role in the pathogenesis of diabetic cardiomyopathy. Apurinic/apyrimidinic endonuclease 1 (APE1) has multiple functions, including regulating redox and DNA repair. However, the role of APE1 in the pathogenesis of DCM remains unclear.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Chemistry, Multidisciplinary
Yecan Pan, Rui Weng, Linghao Zhang, Jing Qiu, Xinlu Wang, Guangqin Liao, Zhaohui Qin, Lingpu Zhang, Haihua Xiao, Yongzhong Qian, Xin Su
Summary: Cellular oxidative stress caused by reactive oxygen species (ROS) and its induced molecular response is crucial in the development of various diseases and biological processes. By using DNA nanostructure-based fluorescent probes, the localization and abundance of ROS and its related molecules can be simultaneously mapped in living cells. This study introduces intramolecular Orthogonal Reporters (iOR) based on DNA nanostructure scaffolds, which eliminate undesired fluorophore interaction by precise positioning of fluorescence reporters. iOR, functionalized with nuclear localization signal peptide, enables high sensitivity and specificity in imaging ROS and its associated DNA repair enzyme (APE1) in the nucleus and cytoplasm. The study reveals a strong positive correlation and synergistic regulation between ROS and APE1 in living cells and tumor-bearing mice through direct fluorescence imaging. This work presents a new approach for developing nanostructure-based molecular probes with broad applications in cellular biology and nanotechnology.
Article
Chemistry, Analytical
Qi Kang, Xiaoyan Yang, Yumin Du, Yinxiao Qi, Zhiqiang He, Hua Xiang
Summary: In this study, a molecular gated hyperbranched rolling circle amplification (HRCA) quick sensing system was designed for rapid detection of APE1. The system exhibited rapid, simple, and sensitive APE1 detection and showed potential therapeutic applications for cancer treatment.
MICROCHEMICAL JOURNAL
(2023)
Article
Immunology
Gerco den Hartog, Lindsay D. Butcher, Amber L. Ablack, Laura A. Pace, Jailal N. G. Ablack, Richard Xiong, Soumita Das, Thaddeus S. Stappenbeck, Lars Eckmann, Peter B. Ernst, Sheila E. Crowe
Summary: APE1 negatively regulates Rac1 to inhibit invasive bacteria from entering human intestinal epithelial cells, protecting the barrier function of epithelial cells.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Shuyu Piao, Ikjun Lee, Seon-Ah Jin, Seonhee Kim, Harsha Nagar, Su-jeong Choi, Byeong Hwa Jeon, Cuk-Seong Kim
Summary: Deletion of CRIF1 in endothelial cells leads to impaired cardiac function, likely due to mitochondrial dysfunction, oxidative stress, inflammation, and decreased expression of SIRT1. The SIRT1 inducer SRT1720 improves cardiac dysfunction by activating endothelial nitric oxide synthase, reducing oxidative stress, and inhibiting inflammation.
Review
Immunology
Seungwha Paik, Jin Kyung Kim, Prashanta Silwal, Chihiro Sasakawa, Eun-Kyeong Jo
Summary: This review article discusses the molecular details of NLRP3 inflammasome activation, focusing on priming/licensing and assembly steps, as well as protein-protein interactions, posttranslational modifications, and spatiotemporal regulation of the NLRP3 inflammasome machinery. Multiple positive and/or negative regulatory pathways providing upstream signals for NLRP3 inflammasome complex assembly are also summarized, with the ultimate goal of developing methods for the prevention and treatment of NLRP3 inflammasome-related diseases.
CELLULAR & MOLECULAR IMMUNOLOGY
(2021)
Article
Medicine, General & Internal
In Seol Yoo, Yu-Ran Lee, Seong Wook Kang, Jinhyun Kim, Hee-Kyoung Joo, Su-Jin Yoo, Chan Keol Park, Ha-Reum Lee, Ji Ah Park, Byeong-Hwa Jeon
Summary: The study demonstrates that the levels of APE1/Ref-1 are significantly elevated in the serum and synovial fluid of rheumatoid arthritis patients, and are positively correlated with disease activity.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Yu-Ran Lee, Eun Young Bae, Hong Ryang Kil, Byeong-Hwa Jeon, Geena Kim
Summary: This study investigated the association between APE1/Ref-1 and Kawasaki disease (KD). The results showed that APE1/Ref-1 levels were significantly higher in KD patients, especially in refractory KD. APE1/Ref-1 could be a beneficial biomarker for the diagnosis and prognosis of KD.
Article
Biochemistry & Molecular Biology
Su-jeong Choi, Harsha Nagar, Jun Wan Lee, Seonhee Kim, Ikjun Lee, Shuyu Piao, Byeong Hwa Jeon, Cuk-Seong Kim
Summary: This study found that IDH2 deficiency induced increased expression of SDC2 in endothelial cells, with MMP7 and TGF-beta signaling playing regulatory roles. UTI could reverse the increase in SDC2, MMP7, and TGF-beta signaling caused by IDH2 deficiency.
Review
Oncology
Wonhyoung Seo, Prashanta Silwal, Ik-Chan Song, Eun-Kyeong Jo
Summary: In this review, the multifaceted functions of autophagy in AML were discussed, including its link to genetic alterations, potential prognostic predictors, and drivers of AML tumorigenesis. The crosstalk between autophagy and tumor cell metabolism and its impact on AML progression and treatment were also examined. Additionally, the potential therapeutics for AML, such as autophagy regulators, were described, along with their translation into clinical practice.
JOURNAL OF HEMATOLOGY & ONCOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Sunga Choi, Yu-Ran Lee, Ki-Mo Kim, Euna Choi, Byeong-Hwa Jeon
Summary: The regulation of inflammatory signaling in the tumor microenvironment using adenoviral-mediated PPTLS-APE1/Ref-1 can inhibit the activity of inflammatory immune cells and cancer cells, providing a potential therapeutic strategy for treating triple-negative breast cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Sungmin Kim, Yu-Ran Lee, Eun-Ok Lee, Hao Jin, Yeon-Hee Choi, Hee-Kyoung Joo, Byeong-Hwa Jeon
Summary: This study found that dietary capsanthin has anti-inflammatory and anti-vascular inflammation effects on atherosclerotic mice, reducing plaque formation and inflammatory marker levels.
Article
Biochemistry & Molecular Biology
Eun-Ok Lee, Hee-Kyoung Joo, Yu-Ran Lee, Sungmin Kim, Kwon-Ho Lee, Sang-Do Lee, Byeong-Hwa Jeon
Summary: Apurinic/apyrimidinic endonuclease 1/redox factor-1 (APE1/Ref-1) is involved in DNA repair and redox regulation. It inhibits adipocyte differentiation by regulating adipogenic transcription factors, suggesting it as a potential therapeutic target for regulating adipocyte differentiation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Shuyu Piao, Ikjun Lee, Seonhee Kim, Hyewon Park, Harsha Nagar, Su-Jeong Choi, Giang-Huong Vu, Minsoo Kim, Eun-Ok Lee, Byeong-Hwa Jeon, Dong Woon Kim, Youngduk Seo, Cuk-Seong Kim
Summary: This study investigates the role of CRIF1 deficiency in modulating mitochondrial oxidative phosphorylation (OXPHOS) system dysfunction and its antitumor effects in MCF-7 breast cancer cells. CRIF1 silencing decreases the assembly of mitochondrial OXPHOS complexes, leading to mitochondrial dysfunction and elevated reactive oxygen species (ROS) levels. Inhibition of CRIF1 suppresses cell proliferation and inhibits cell migration in MCF-7 cells, and intratumoral injection of CRIF1 siRNA nanoparticles inhibits tumor growth in MCF-7 xenograft mice.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Young Jae Kim, Eun-Jin Park, Sang-Hee Lee, Prashanta Silwal, Jin Kyung Kim, Jeong Seong Yang, Jake Whang, Jichan Jang, Jin-Man Kim, Eun-Kyeong Jo
Summary: In this study, dimethyl itaconate (DMI) was found to have potential as a candidate for host-directed therapy (HDT) against both Mycobacterium tuberculosis (Mtb) and nontuberculous mycobacteria. It can activate multiple immune defense mechanisms in the host to promote intracellular killing of mycobacteria and suppress inflammation. This research contributes to the development of new anti-tuberculosis drugs.
CELL AND BIOSCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Ikjun Lee, Shuyu Piao, Seonhee Kim, Harsha Nagar, Su-jeong Choi, Minsoo Kim, Giang-Huong Vu, Byeong-Hwa Jeon, Cuk-Seong Kim
Summary: Endothelial senescence impairs vascular function and is a major factor in age-related vasculature diseases. IDH2 is involved in the production of alpha-ketoglutarate and preservation of mitochondrial function. However, the mechanism and regulation of IDH2 in endothelial senescence have not been fully understood.
Article
Pharmacology & Pharmacy
Thi Hoa Pham, Gi Ho Lee, Sun Woo Jin, Seung Yeon Lee, Eun Hee Han, Nam Doo Kim, Chul Yung Choi, Gil-Saeng Jeong, Sang Ki Lee, Hyung Sik Kim, Hye Gwang Jeong
Summary: This study found that sesamin can effectively reduce lipid accumulation, alleviate lipotoxicity, and improve lipid metabolism imbalance. Additionally, sesamin can regulate key factors involved in lipogenesis and lipolysis by activating specific signaling pathways. These findings suggest that sesamin may be a promising candidate for treating hepatic steatosis.
BIOCHEMICAL PHARMACOLOGY
(2023)
Article
Medicine, General & Internal
Yumin Jo, Yeojung Kim, Eunhye Park, Yuran Lee, Jiyeon Kim, Minwoong Kang, Jaesung Lim, Insang Song, Chaeseong Lim, Byeonghwa Jeon
Summary: The study found that the plasma APE1/Ref-1 level of patients undergoing cancer surgery increases and is related to the degree of lymph node invasion, but is not affected by the choice of different anesthetics.
MEDICINA-LITHUANIA
(2021)
Article
Nutrition & Dietetics
Ki Mo Kim, Joo Young Lee, Byeong Hwa Jeon, Khong Trong Quan, MinKyun Na, Kung-Woo Nam, Sungwook Chae
Summary: The study showed that dietary intake of Curcuma zedoaria extract reduced atherosclerosis in mice, along with decreasing serum triglyceride and high-density lipoprotein levels, and reducing inflammatory molecule expression.
NUTRITION RESEARCH AND PRACTICE
(2021)