Article
Microbiology
Teck-Phui Chua, Jennifer Danielewski, Kaveesha Bodiyabadu, Catriona S. Bradshaw, Dorothy A. Machalek, Suzanne M. Garland, Erica L. Plummer, Lenka A. Vodstrcil, Gerald L. Murray
Summary: This study investigates the association between single nucleotide polymorphisms (SNPs) in the 16S rRNA gene of Mycoplasma genitalium and change in organism load. Though doxycycline is not highly effective at eradicating M. genitalium infections, it can reduce organism load. The study concludes that the low efficacy of doxycycline against M. genitalium is not due to variation in the 16S rRNA gene.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Microbiology
Miguel Blanco-Fuertes, Florencia Correa-Fiz, Lorenzo Fraile, Marina Sibila, Virginia Aragon
Summary: This study highlights the role of nasal microbiota in subsequent systemic infection by Mycoplasma hyorhinis and the differences between this infection and Glasser's disease. Animals from disease farms had lower diversity in their nasal microbiota compared to healthy farms, but the composition was different between the two diseases, indicating divergent microbiota imbalances may predispose animals to different systemic infections.
Article
Immunology
Yacui Wang, Xiaoran Yu, Fang Liu, Xue Tian, Shuting Quan, Anxia Jiao, Xuemei Yang, Xi Zeng, Weiwei Jiao, Hui Qi, Fang Xu, Qinjing Li, Shuping Liu, Baoping Xu, Lin Sun, Adong Shen
Summary: We analyzed the microbial community in bronchoalveolar lavage fluid samples from children with Mycoplasma pneumoniae pneumonia (MPP) and found significant differences in diversity compared to a control group. The abundance of Tenericutes and Mycoplasma was significantly increased in the MPP group. Using Mycoplasma abundance as a diagnostic method, we achieved high sensitivity and specificity. Our study also revealed a correlation between Mycoplasma abundance and disease severity in children with MPP.
EMERGING MICROBES & INFECTIONS
(2023)
Article
Microbiology
Matthew Waldner, Andrea Kinnear, Elhem Yacoub, Tim McAllister, Karen Register, Changxi Li, Murray Jelinski
Summary: This study used a genome-wide association study (GWAS) approach to investigate the antimicrobial resistance (AMR) of Mycoplasma bovis, identifying nucleotide variants (NVs) associated with resistance to different classes of antimicrobials. The study concluded that AMR in M. bovis is influenced by multiple factors, including NVs in genes coding for membrane proteins, tRNA-ligases, peptidases, and transposases.
Article
Biochemistry & Molecular Biology
Shohei Akagawa, Yuko Akagawa, Sohsaku Yamanouchi, Yoshiki Teramoto, Masahiro Yasuda, Sadayuki Fujishiro, Jiro Kino, Masato Hirabayashi, Kenji Mine, Takahisa Kimata, Masaki Hashiyada, Atsushi Akane, Shoji Tsuji, Kazunari Kaneko
Summary: Neonatal jaundice, caused by excess serum bilirubin levels, is a common condition in neonates. Imbalance in gut microbiota may play a role in its development, with dysbiosis characterized by decreased abundance of Bifidobacteriales found in neonates with jaundice.
Article
Parasitology
Sandor Hornok, Jeno Kontschan, Nora Takacs, Heloise Heyne, Aron Botond Kovacs, Olivier Plantard, Gergo Keve, Denis Fedorov, Miklos Gyuranecz, Ali Halajian
Summary: The genus Ixodes has the highest number of species among hard ticks, and the majority of them are found in the Afrotropical region. This study analyzed 58 Ixodes spp. ticks collected from mammalian and avian hosts in South Africa using molecular and phylogenetic methods. The results identified 11 morphologically distinct tick species, with the majority belonging to the Ixodes pilosus group. Molecular analyses confirmed the presence of two species in this group and discovered a new species closely related to I. rubicundus. Phylogenetic analyses also revealed the relationships between different subgenera of Ixodes ticks.
PARASITES & VECTORS
(2023)
Article
Multidisciplinary Sciences
Kazuaki Matsui, Takeshi Miki
Summary: When there is excessive wastewater volume during extreme rainfall, untreated sewage is discharged into rivers as combined sewer overflow (CSO). This study compared the impacts of CSOs and stormwater on urban waterways by assessing physicochemical water quality, bacterial community structure, and microbial functions. The results showed that CSOs significantly affected microbial function and bacterial community structure, but had no confirmed impact on physicochemical parameters, bacterial abundance, and chlorophyll a. The implementation of a stormwater storage pipe alleviated CSO-induced microbial disturbances and supported efficient waterway management in urban areas.
Article
Biochemistry & Molecular Biology
Nabeel Merali, Tarak Chouari, Julien Terroire, Maria-Danae Jessel, Daniel S. K. Liu, James-Halle Smith, Tyler Wooldridge, Tony Dhillon, Jose I. Jimenez, Jonathan Krell, Keith J. Roberts, Timothy A. Rockall, Eirini Velliou, Shivan Sivakumar, Elisa Giovannetti, Ayse Demirkan, Nicola E. Annels, Adam E. Frampton
Summary: The bile microbiome plays an important role in pancreatic ductal adenocarcinoma (PDAC) and can distinguish malignant tumors from benign ones. It was found that the composition of the bile microbiome is altered in patients with PDAC, suggesting that these microbial changes could potentially serve as diagnostic and prognostic biomarkers for PDAC.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Infectious Diseases
Marllon Barbosa, Patricia G. Paulino, Tays A. Camilo, Debora Martins, Leo Paulis, Nathalia A. Senne, Olga Lucia H. Ramirez, Isabele C. Angelo, Carlos L. Massard, Huarrisson A. Santos
Summary: This study investigated the epidemiology and spatial distribution of hemotropic Mycoplasma spp. and Mycoplasma haemocanis in household dogs from Rio de Janeiro, Brazil, revealing infection rates and factors associated with the presence of these pathogens in dogs. The findings also suggest a potential vector for M. haemocanis and provide insights for prevention of Mycoplasma spp. infection in dogs in this region.
INFECTION GENETICS AND EVOLUTION
(2021)
Article
Microbiology
Dandan Yu, Jinru Yang, Min Jin, Bin Zhou, Linli Shi, Lei Zhao, Jieying Zhang, Zhenyu Lin, Jinghua Ren, Li Liu, Tao Zhang, Hongli Liu
Summary: The gut microbiome plays a critical role in predicting gastric cancer and liver metastasis, with Streptococcus potentially serving as a biomarker.
Article
Ecology
Robert Starke, Victor Satler Pylro, Daniel Kumazawa Morais
Summary: Sequencing 16S rRNA gene amplicons is commonly used to study prokaryotic communities, but gene copy normalization (GCN) does not improve the accuracy of results. The study questions the use of GCN for metataxonomic surveys until a more comprehensive catalogue of copy numbers becomes available.
Article
Fisheries
Manoharmayum Shaya Devi, Prasenjit Paria, Vikash Kumar, Pranaya Kumar Parida, Praveen Maurye, Bijay Kumar Behera, Basanta Kumar Das
Summary: This study identified a non-pathogenic serotype of Vibrio cholerae isolated from an Indian wetland system, emphasizing the importance of proper management in preventing diseases in aquaculture.
Article
Marine & Freshwater Biology
Zhen Wu, Xiaohong Yang, Senjie Lin, Wai Hin Lee, Paul K. S. Lam
Summary: A study identified a Rhizobium species from a marine toxic dinoflagellate Gambierdiscus culture for the first time, suggesting a potential physiological interaction between the dinoflagellate and the bacterium as its abundance increased during the late exponential growth phase and under nitrogen limitation. Further investigation is needed to explore the prevalence and ecological roles of dinoflagellate-associated Rhizobium bacterium.
MARINE LIFE SCIENCE & TECHNOLOGY
(2021)
Article
Microbiology
Jasmin L. Millar, Elizabeth A. Bagshaw, Arwyn Edwards, Ewa A. Poniecka, Anne D. Jungblut
Summary: Cryoconite holes, containing water and microbe-mineral aggregates, are hotspots of microbial diversity on glacial surfaces. They form in various conditions and locations, impacting their structure and community composition. The bacterial and microalgal communities in Arctic and Antarctic cryoconite holes show clear divisions, with regionally distinct microbial communities adapted to differing conditions within the cryosphere.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Oncology
Anchao Zhu, Yingying Liu, Zongmin Li, Ying He, Lijing Bai, Youtian Wu, Yuying Zhang, Ying Huang, Ping Jiang
Summary: This study assessed the microbial composition and diversity of SCRC tumors with varying MLH1 status, and investigated the effects of functional genes on bacterial markers and clinical diagnostic prediction. The results identified Lachnoclostridium and Coprococcus as key species that may serve as crucial biomarkers for non-invasive diagnostic prediction of DM in patients with SCRC.
FRONTIERS IN ONCOLOGY
(2023)
Article
Immunology
Aleksandar Potkonjak, Alicia Rojas, Ricardo Gutierrez, Yaarit Nachum-Biala, Gabriela Kleinerman, Sara Savic, Vladimir Polacek, Ivan Pusic, Shimon Harrus, Gad Baneth
COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES
(2020)
Article
Microbiology
Ricardo Gutierrez, Tali Shalit, Barak Markus, Congli Yuan, Yaarit Nachum-Biala, Daniel Elad, Shimon Harrus
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2020)
Article
Infectious Diseases
Luiz Ricardo Goncalves, Shimon Harrus, Ricardo Gutierrez, Heitor Miraglia Herrera, Inalda Angelica de Souza Ramos, Grasiela Edith de Oliveira Porfirio, Yaarit Nachum-Biala, Keyla Carstens Marques de Sousa, Thiago Merighi Vieira da Silva, Joao Bosco Vilela Campos, Wagner Lemos, Darci Moraes Barros-Battesti, Rosangela Zacarias Machado, Marcos Rogerio Andre
TRANSBOUNDARY AND EMERGING DISEASES
(2020)
Article
Microbiology
Kensuke Shima, Mary M. Weber, Christiane Schnee, Konrad Sachse, Nadja Kaeding, Matthias Klinger, Jan Rupp
Article
Veterinary Sciences
Godelind Alma Wolf-Jackel, Mikael Lenz Strube, Kirstine Klitgaard Schou, Christiane Schnee, Jorgen S. Agerholm, Tim Kare Jensen
Summary: This study aimed to identify potentially abortifacient bacteria without bias, increase diagnostic rate using molecular methods, and investigate the presence of difficult-to-culture zoonotic agents in bovine abortions in Denmark. The use of 16S rDNA amplicon sequencing-guided FISH approach was found to enhance abortion diagnostics, leading to an increased diagnostic rate for cases with tissue lesions compared to routine methods. No evidence of emerging or epizootic bacterial pathogens was found.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Microbiology
Alison Favaroni, Alexander Trinks, Michael Weber, Johannes H. Hegemann, Christiane Schnee
Summary: Chlamydia psittaci, the etiological agent of chlamydiosis in birds, can also infect humans, causing severe systemic disease. Variation in Pmps, especially the G group, may contribute to the host tropism of avian and mammalian C. psittaci strains. Adhesins like Pmp22D, Pmp8G, and OmcB play essential roles in infection, while Pmp17G may be a key player in host adaptation to avian cells.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Fabien Vorimore, Rachid Aaziz, Bertille de Barbeyrac, Olivia Peuchant, Monika Szymanska-Czerwinska, Bjorn Herrmann, Christiane Schnee, Karine Laroucau
Summary: C. psittaci is the causative agent of avian chlamydiosis and human psittacosis. A PCR-HRM assay was developed to screen for phylogenetically informative SNPs related to the identified C. psittaci lineages, allowing quick identification of the source of infection. The method was validated with reference strains and field isolates, and was consistent with previous genotyping data.
Article
Infectious Diseases
Oystein Angen, Thor Bech Johannesen, Randi Fons Petersen, Soren Anker Uldum, Christiane Schnee
Summary: A species-specific real-time PCR assay for Chlamydia psittaci has been developed based on an 84 bp indel in a gene unique to C. psittaci. The assay showed excellent test characteristics and high reliability in identifying C. psittaci in clinical samples from both animals and humans. Overall, this new PCR test offers a promising method for the detection of C. psittaci.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2021)
Article
Microbiology
Rosemonde Isabella Power, Nichola Elisa Davies Calvani, Yaarit Nachum-Biala, Harold Salant, Shimon Harrus, Jan Slapeta
Summary: This study demonstrates the use of Next Generation Sequencing (NGS) for accurate identification of Bartonella species in fleas from different hosts. The study shows that NGS is superior to traditional Sanger sequencing in resolving mixed Bartonella infections, and has the potential for future studies with mixed bacterial infections.
JOURNAL OF MEDICAL MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Ricardo Gutierrez, Yoav Ram, Judith Berman, Keyla Carstens Marques de Sousa, Yaarit Nachum-Biala, Malka Britzi, Daniel Elad, Gad Glaser, Shay Covo, Shimon Harrus
Summary: The emergence of resistant bacteria during antimicrobial treatment is a critical health threat, stress-induced mutagenesis and heteroresistance mechanisms can enhance microbial adaptation to antibiotics. Bartonella can acquire single mutations at a high rate during rifampicin exposure, while exhibiting minimal heteroresistance capacity. This highlights the importance of monitoring and controlling antibiotic use to prevent the development of resistant strains.
MOLECULAR BIOLOGY AND EVOLUTION
(2021)
Review
Biochemistry & Molecular Biology
Ruth Rodriguez-Pastor, Yarden Shafran, Nadav Knossow, Ricardo Gutierrez, Shimon Harrus, Luis Zaman, Richard E. Lenski, Jeffrey E. Barrick, Hadas Hawlena
Summary: This article introduces laboratory experiments on the evolution of blood-borne parasitic microbes in animal hosts, offering guidelines for designing such experiments. The importance of selecting appropriate ancestral genotypes, treatments, replicates, controls, variables, covariates, and timing for checkpoints is emphasized, along with recommended preliminary experiments for quantification and transmission methods. Despite their technical nature, these methodological considerations also have conceptual implications.
MOLECULAR ECOLOGY RESOURCES
(2022)
Article
Agriculture, Dairy & Animal Science
Francisco Rodriguez, Ruben S. Rosales, Ana S. Ramirez, Jose B. Poveda
Summary: Mycoplasma hyopneumoniae is the causative agent of enzootic pneumonia in pigs, leading to impaired immune-inflammatory function and fatal pneumonia. Vaccination is commonly used, but its effectiveness varies. Enhancing Th1 response is crucial to improve vaccine efficacy.
Article
Parasitology
Jan Slapeta, Bruce Halliday, Jason A. Dunlop, Yaarit Nachum-Biala, Harold Salant, Sajjad Ghodrati, David Modry, Shimon Harrus
Summary: This study clarifies the identity of the southeastern Europe lineage of the Rhipicephalus sanguineus tick species complex, revealing that it corresponds to the morphology of R. rutilus. The researchers characterized the complete mitogenomes of R. rutilus, R. turanicus, and R. sanguineus to improve our understanding of the phylogenetic relationships within this tick species complex. The study concludes that the name R. rutilus is correctly linked to the southeastern Europe lineage of R. sanguineus.
CURRENT RESEARCH IN PARASITOLOGY & VECTOR-BORNE DISEASES
(2023)
Article
Microbiology
Martin Hoelzer, Lisa-Marie Barf, Kevin Lamkiewicz, Fabien Vorimore, Marie Lataretu, Alison Favaroni, Christiane Schnee, Karine Laroucau, Manja Marz, Konrad Sachse
Article
Veterinary Sciences
A. Berkowitz, S. Harrus, R. Gutierrez, Y. Nachum-Biala, A. Bouznach, E. Green, A. Davidoff, S. Klainbart, T. Waner
ISRAEL JOURNAL OF VETERINARY MEDICINE
(2020)
