Article
Microbiology
Sarah Hendrickx, Joao Luis Reis-Cunha, Sarah Forrester, Daniel C. Jeffares, Guy Caljon
Summary: The study identified potential genetic variations associated with paromomycin resistance in Leishmania, including genes involved in transcription, translation, protein turnover, virulence, mitochondrial function, signaling, and vesicular trafficking. These results suggest that paromomycin affects protein translational processes and highlights the complex and multifactorial origin of resistance in Leishmania.
Article
Microbiology
Bart Cuypers, Pieter Meysman, Ionas Erb, Wout Bittremieux, Dirk Valkenborg, Geert Baggerman, Inge Mertens, Shyam Sundar, Basudha Khanal, Cedric Notredame, Jean-Claude Dujardin, Malgorzata A. Domagalska, Kris Laukens
Summary: Aneuploidy has a significant impact on the transcriptome, proteome, and metabolome of Leishmania donovani, leading to disruptions in stochiometric balances and metabolic differences between aneuploid strains. However, protein complex subunits and non-cytoplasmic proteins show attenuations in dosage effects. The differentially expressed proteins are enriched for those involved in protein metabolism. These findings suggest that aneuploidy in Leishmania can be adaptive and make it an attractive model for studying protein homeostasis in the context of aneuploidy.
Review
Plant Sciences
Qi Zheng, Vera Putker, Aska Goverse
Summary: Cyst nematodes pose a major threat to crop production by infecting a wide range of crop species. Plants activate their innate immune system to defend against cyst nematodes by conferring basal and host-specific defense responses. The use of R genes and QTLs is a major approach to control cyst nematodes in crop cultivation, but the emergence of virulent field populations threatens crop production, highlighting the importance of studying host-specific resistance mechanisms.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Immunology
Johannes S. P. Doehl, Helen Ashwin, Najmeeyah Brown, Audrey Romano, Samuel Carmichael, Jon W. Pitchford, Paul M. Kaye
Summary: Research indicates that in hosts infected with parasites of the Leishmania donovani complex, heavily parasite-infected myeloid cells are found in patches in the skin, from which the parasites spread to other cells. The combination of imaging and ecological pattern analysis offers new insights into understanding the behavior of myeloid cells following parasitism by L. donovani and potentially other intracellular tissue-resident pathogens.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Multidisciplinary Sciences
Aretha Chan, Jose-Mauricio Ayala, Fernando Alvarez, Ciriaco Piccirillo, George Dong, David Langlais, Martin Olivier
Summary: This study analyzed the early innate inflammatory events during L. major infection, confirming the significant role of the virulence factor GP63 in providing favorable conditions for host cell infection. The data suggest that GP63 does not greatly influence pathogen-induced inflammatory cell recruitment, but rather affects their activation status and effector function, as well as potentially influencing the internalization of promastigotes during early infection.
Article
Parasitology
Sushmita Ghosh, Vinay Kumar, Aditya Verma, Tanya Sharma, Dibyabhaba Pradhan, Angamuthu Selvapandiyan, Poonam Salotra, Ruchi Singh
Summary: Leishmaniasis is a major global public health concern and drug resistance is a key obstacle in controlling and eliminating this disease. This study compared the whole-genome sequence of a drug-resistant parasite with a sensitive clinical isolate, and found a large number of gene variations in the resistant parasite. These variations may have led to rapid evolutionary changes.
PARASITOLOGY RESEARCH
(2022)
Article
Microbiology
Emily Yang, Serina Huang, Yasaman Jami-Alahmadi, Gerald M. McInerney, James A. Wohlschlegel, Melody M. H. Li
Summary: The TRIM family of E3 ubiquitin ligases, especially TRIM25, plays important roles in antiviral response and cellular pathways. A specific mutation, R54P, is critical for TRIM25's catalytic activity and can trap substrates. TRIM25 targets proteins involved in stress granule formation, mRNA decay, nucleoside synthesis, and mRNA translation and stability. The R54P mutation eliminates TRIM25's inhibition of alphaviruses independently of the host interferon response, indicating that this antiviral effect is directly related to ubiquitination. The study demonstrates the diverse biological processes regulated by TRIM25 and highlights the multi-faceted role of ubiquitination network.
Review
Neurosciences
Constantino Sotelo
Summary: Over the past 50 years, our understanding of the cerebellum has evolved significantly, from the description of extracerebellar projections' terminations by Voogd to the discovery of the biochemical heterogeneity of Purkinje cells by Hawkes and colleagues, leading to a more complex subdivision of the cerebellar cortex into precise longitudinal compartments.
Article
Immunology
Keerti, Narendra Kumar Yadav, Sumit Joshi, Sneha Ratnapriya, Amogh Anant Sahasrabuddhe, Anuradha Dube
Summary: This study demonstrates the effectiveness of a chemo-immunotherapeutic strategy in improving immune response and reducing parasitic load in visceral leishmaniasis. The combination of recombinant proteins and a low dose of antileishmanial drug shows promise for controlling the infection with minimal side effects and drug resistance emergence.
JOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTION
(2023)
Article
Immunology
Rahat Ali, Shams Tabrez, Sajjadul Kadir Akand, Fazlur Rahman, Atahar Husein, Mohd Arish, Ali S. Alqahtani, Mohammad Z. Ahmed, Mohammad Husain, Abdur Rub
Summary: This study evaluated the antileishmanial potential of sesamol against L. donovani, showing that sesamol inhibits parasite growth and proliferation through induction of reactive oxygen species (ROS), mitochondrial dysfunction, DNA fragmentation, cell cycle arrest, and apoptosis-like cell death. The results suggest the possible use of sesamol for the treatment of leishmaniasis following further in vivo validations.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Parool Gupta, Rani Mansuri, Priya Priydarshni, Sachidananda Behera, Amir Zaidi, Shamshun Nehar, Ganesh Chandra Sahoo, Krishna Pandey, Vahab Ali
Summary: This study revealed the interaction between two proteins, Cfd1 and Nbp35, in L. donovani, forming a stable complex involved in the cellular process of Fe-S cluster biogenesis and transfer. The upregulation of Cfd1 and Nbp35 proteins in drug-resistant parasites leads to increased Fe-S protein levels, promoting parasite survival under drug pressure.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Plant Sciences
Wei Deng, Zhiwen Duan, Yang Li, Cheng Peng, Shuzhong Yuan
Summary: This study investigated the resistance levels and potential resistance mechanisms of Eleusine indica biotypes to glyphosate, a widely used herbicide in non-cultivated land in China. The study found that EPSPS gene overexpression and mutation were common resistance mechanisms. In addition, candidate genes involved in non-target-site resistance were identified.
Article
Immunology
Amrita Das, Mohd. Kamran, Nahid Ali
Summary: The study demonstrates that the liposomal formulation of HO-3867 is effective in inducing apoptosis in Leishmania parasites while being safe for normal murine macrophages. The treatment effectively reduces intracellular L. donovani amastigote burden by inducing parasite killing through ROS and NO activity.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Review
Medicine, General & Internal
Iris Bararu Bojan (Bararu), Carmen Elena Plesoianu, Oana Viola Badulescu, Maria Cristina Vladeanu, Minerva Codruta Badescu, Dan Iliescu, Andrei Bojan, Manuela Ciocoiu
Summary: Aortic aneurysms are a common pathology affecting different segments of the aorta. The article reviews the mechanisms leading to the development of aortic aneurysms and explores different treatment options. Surgical management remains the most common approach, despite the associated risks.
Review
Biochemistry & Molecular Biology
Gaetano Caramori, Francesco Nucera, Sharon Mumby, Federica Lo Bello, Ian M. Adcock
Summary: Inhaled glucocorticoids (GCs) are widely used to treat asthma patients. They prevent the activation of immune and inflammatory cells in the lungs and have significant effects on airway structure. However, some patients show poor asthma control even with high doses of GCs, which may be due to GC resistance.
MOLECULAR ASPECTS OF MEDICINE
(2022)
Article
Infectious Diseases
Neena George, Vasundhra Bhandari, Paresh Sharma
INFECTION GENETICS AND EVOLUTION
(2017)
Article
Parasitology
Aditya Verma, Vasundhra Bhandari, Deepak Kumar Deep, Shyam Sundar, Jean Claude Dujardin, Ruchi Singh, Poonam Salotra
INTERNATIONAL JOURNAL FOR PARASITOLOGY-DRUGS AND DRUG RESISTANCE
(2017)
Review
Immunology
Shalini Chakraborty, Sonti Roy, Hiral Uday Mistry, Shweta Murthy, Neena George, Vasundhra Bhandari, Paresh Sharma
FRONTIERS IN IMMUNOLOGY
(2017)
Review
Immunology
Veena Kumari Haradara Bahubali, Priya Vijayan, Vasundhra Bhandari, Nagarathna Siddaiah, Dwarakanath Srinivas
INDIAN JOURNAL OF MEDICAL MICROBIOLOGY
(2018)
Article
Multidisciplinary Sciences
Umarani Brahma, Richa Kothari, Paresh Sharma, Vasundhra Bhandari
SCIENTIFIC REPORTS
(2018)
Article
Chemistry, Physical
Praveen Chapala, Pandu Sunil Kumar, Joydip Joardar, Vasundhra Bhandari, Swati Ghosh Acharyya
APPLIED SURFACE SCIENCE
(2019)
Article
Multidisciplinary Sciences
Debabrata Dandasena, Vasundhra Bhandari, G. S. Sreenivasamurthy, Shweta Murthy, Sonti Roy, Vandna Bhanot, Jaspreet Singh Arora, Satparkash Singh, Paresh Sharma
SCIENTIFIC REPORTS
(2018)
Article
Immunology
Vasundhra Bhandari, Shalini Chakraborty, Umarani Brahma, Paresh Sharma
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2018)
Article
Biophysics
Suryasnata Tripathy, Vasundhra Bhandari, Paresh Sharma, Siva Rama Krishna Vanjari, Shiv Govind Singh
BIOSENSORS & BIOELECTRONICS
(2019)
Article
Infectious Diseases
Gajanand Mittal, Vasundhra Bhandari, Rajni Gaind, Vandana Rani, Shimpi Chopra, Reetika Dawar, Raman Sardana, P. K. Verma
BMC INFECTIOUS DISEASES
(2019)
Article
Physiology
Sonti Roy, Vasundhra Bhandari, Debabrata Dandasena, Shweta Murthy, Paresh Sharma
FRONTIERS IN PHYSIOLOGY
(2019)
Article
Multidisciplinary Sciences
Umarani Brahma, Paresh Sharma, Shweta Murthy, Savitri Sharma, Shalini Chakraborty, Sundarapu Naga Appalaraju, Vasundhra Bhandari
SCIENTIFIC REPORTS
(2019)
Correction
Multidisciplinary Sciences
Hiral Mistry, Paresh Sharma, Sudipta Mahato, R. Saravanan, P. Anand Kumar, Vasundhra Bhandari
Review
Biochemistry & Molecular Biology
Namrata Devsani, Divya Vemula, Vasundhra Bhandari
Summary: Leishmaniasis is a tropical parasitic disease caused by Leishmania spp., and it presents in different forms ranging from cutaneous to visceral leishmaniasis. The current drugs for treating leishmaniasis are limited, and drug resistance is a major issue. Therefore, exploring new treatment targets is necessary, and gp63, a glyco-protein that plays a significant role in host-pathogen interaction and virulence, is being investigated as a potential target. This review discusses the potential of gp63 as a drug target and summarizes studies on gp63 inhibitors identified through computational methods.
Article
Biochemistry & Molecular Biology
Siva Singothu, Pathan J. Begum, Dhanashri Maddi, Namrata Devsani, Vasundhra Bhandari
Summary: This study identifies potential LasI inhibitors from a marine compound database using molecular docking and simulation techniques. Out of 10 compounds, two demonstrate consistent stability in protein-ligand complex throughout a 100 ns simulation. These compounds show promise for further validation as LasI inhibitors through in vitro and in vivo experiments.
JOURNAL OF CELLULAR BIOCHEMISTRY
(2023)