Review
Biochemistry & Molecular Biology
Gabriela Vieira Silva Zolin, Fauller Henrique da Fonseca, Carolina Reis Zambom, Saulo Santesso Garrido
Summary: Buccal candidiasis, caused by Candida albicans, can lead to systemic infection with resistance to common antifungal drugs. Histatin 5, a natural antimicrobial peptide found in human saliva, shows high antifungal activity against C. albicans. Research aims to explore the promising use of Hst5 metallopeptides in developing effective drugs to overcome antifungal resistance.
Article
Genetics & Heredity
Parisa Moghaddam-Taaheri, Jesse A. Leissa, Haleigh B. Eppler, Christopher M. Jewell, Amy J. Karlsson
Summary: The study demonstrates the therapeutic potential of the K11R-K17R Hst-5 variant in preventing and treating Candida albicans biofilms.
FUNGAL GENETICS AND BIOLOGY
(2021)
Article
Microbiology
Pratibha Sharma, Mehak Chaudhary, Garima Khanna, Praveen Rishi, Indu Pal Kaur
Summary: Histatin 5 (Hstn 5), an antimicrobial peptide found in saliva, exhibits fungicidal activity against various fungi and shows potential as a novel approach for controlling fungal infections.
Article
Chemistry, Medicinal
Joanna X. Campbell, Sean Gao, Keerthi S. Anand, Katherine J. Franz
Summary: This study investigates the role of Zn2+ in regulating the antifungal activity of Hist-5. Increased Zn2+ concentration prevents peptide internalization, reducing cytotoxicity and membrane disruption. The activity of Hist-5 can be rescued by decreasing the availability of Zn2+.
ACS INFECTIOUS DISEASES
(2022)
Article
Cell Biology
Bowen Zhang, Xin Qin, Mi Zhou, Taoran Tian, Yue Sun, Songhang Li, Dexuan Xiao, Xiaoxiao Cai
Summary: The study successfully synthesized TDN/His-5, demonstrating that modification by TDNs increased transport efficiency and improved anti-fungal effect of His-5.
CELL PROLIFERATION
(2021)
Article
Biotechnology & Applied Microbiology
Rui Li, Mengjie Hou, Liying Yu, Wen Luo, Jie Kong, Renmei Yu, Ruihan Liu, Qian Li, Lisi Tan, Chunling Pan, Hongyan Wang
Summary: This study aimed to investigate the effects of salivary histatin 5 (Hst5) on Porphyromonas gingivalis (P. gingivalis) biofilms in vitro and in vivo and the possible mechanisms. The results showed that 25μg/mL Hst5 effectively inhibited biofilm formation and increased concentrations of Hst5 increased the inhibitive effect. Hst5 could regulate membrane function and metabolic processes in P. gingivalis, and RpoD and FeoB proteins might play important roles in this process. Moreover, 100μg/mL Hst5 inhibited periodontal inflammation and alveolar bone loss in rat periodontitis through its antibacterial and anti-inflammatory effects.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Infectious Diseases
Yongqin Wu, Yisheng Chen, Huaiwei Lu, Chunmei Ying
Summary: Invasive candidiasis is a common and serious fungal disease, and drug resistance in Candida spp. is a growing problem. Miltefosine, an approved drug for invasive candida infections, has shown good antifungal activity but its mechanism of action is unclear. This study found that miltefosine increased intracellular reactive oxygen species (ROS) and induced apoptosis in Candida albicans. Through various analyses, Aif1 and the oxidative stress pathway were identified as the mechanisms of miltefosine's action. These findings improve our understanding of how miltefosine affects fungi.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2023)
Article
Chemistry, Multidisciplinary
Maisa Santos Fonseca, Daniela Meria Rodrigues, Ana Rita Sokolonski, Danijela Stanisic, Luiz Marcelo Tome, Aristoteles Goes-Neto, Vasco Azevedo, Roberto Meyer, Danilo Barral Araujo, Ljubica Tasic, Ricardo Dias Portela
Summary: This study evaluated the activity of Fusarium oxysporum-based silver nanoparticles (AgNPs) on Candida spp. isolated from denture stomatitis lesions and investigated their interference on biofilm formation and mechanisms of action. The results showed that AgNPs were effective against most Candida spp. and could potentially be a new therapeutic agent against oral candidiasis.
Article
Genetics & Heredity
Saif Hossain, Amanda O. Veri, Zhongle Liu, Kali R. Iyer, Teresa R. O'Meara, Nicole Robbins, Leah E. Cowen
Summary: The study found that the functionality of mitochondria in Candida albicans is closely related to the impact of Hsp90 inhibitors on morphogenesis, with disruption of mitochondrial function reducing sensitivity to the inhibitors. Further research revealed that mutations in certain genes can increase cellular efflux and decrease susceptibility to compounds. Deletion of specific transporter genes can restore sensitivity to Hsp90 inhibitors, enabling filamentation.
Article
Nanoscience & Nanotechnology
Bernhard Friedrich, Rainer Tietze, Michaela Duemig, Alexandru Sover, Marius-Andrei Boca, Eveline Schreiber, Julia Band, Christina Janko, Sven Krappmann, Christoph Alexiou, Stefan Lyer
Summary: Magnetic nanoparticles have been successfully utilized for rapid sample preparation and separation of pathogens, such as Candida albicans, for diagnostic purposes. By functionalizing the nanoparticles with a suitable peptide, the magnetic separation method ensures selective enrichment of the target microorganism without compromising the integrity of human blood cells.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Simona Vaitkiene, Neringa Kuliesiene, Sandra Sakalauskaite, Laura Bekere, Laura Krasnova, Brigita Vigante, Gunars Duburs, Rimantas Daugelavicius
Summary: Styrylpyridinium compounds showed antifungal activity against C. albicans cells, some exhibited synergism with fluconazole, but also posed toxicity to mammalian cells.
CHEMICAL BIOLOGY & DRUG DESIGN
(2021)
Article
Biochemistry & Molecular Biology
Jakub Suchodolski, Jakub Muraszko, Przemyslaw Bernat, Anna Krasowska
Summary: Studies have shown that lactate can reduce Candida albicans' resistance to fluconazole by affecting the expression of the ERG11 gene and ergosterol content, leading to the delocalization of the Cdr1 protein and inhibition of efflux.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Pharmacology & Pharmacy
Hongkang Wang, Zhe Ji, Yanru Feng, Tianhua Yan, Yongbing Cao, Hui Lu, Yuanying Jiang
Summary: Inhibiting sphingolipid biosynthesis to block membrane localization and inactivate Cdr1 was found to enhance the antifungal activity of azoles against azole-resistant C. albicans, suggesting its therapeutic potential.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Microbiology
Zhongjie Li, Xiaoyuan Jing, Yaping Yuan, Yingbin Shui, Shasha Li, Zhuoran Zhao, Bo Deng, Wenlu Zhang
Summary: Phibilin, a new antimicrobial peptide obtained from Philomycus bilineatus, effectively inhibits the growth and activities of antifungal-resistant Candida albicans. It acts as a fungicidal peptide by disrupting the membrane and interacting with the DNA of the fungi. Moreover, Phibilin induces necrosis in C. albicans via the ROS-related pathway and inhibits biofilm formation. In addition, it displays a synergistic effect with clotrimazole and shows promising results in a mouse cutaneous infection model.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Infectious Diseases
Jie Hou, Jin Deng, Ya Liu, Weili Zhang, Siying Wu, Quanfeng Liao, Ying Ma, Mei Kang
Summary: The aim of this study was to investigate the current status of candidemia and evaluate the clinical characteristics, risk factors, and outcomes among different species. The study found variations in the prevalence of Candida albicans and non-albicans Candida and differences in the susceptibility to antifungal agents among different species. Risk factors associated with Candida albicans and non-albicans Candida were identified, and no significant differences in mortality were observed between the two species.
Article
Immunology
Rohitashw Kumar, Darpan Saraswat, Swetha Tati, Mira Edgerton
INFECTION AND IMMUNITY
(2015)
Review
Biochemistry & Molecular Biology
Darpan Saraswat, Rohitashw Kumar, Tanaya Pande, Mira Edgerton, Paul J. Cullen
MOLECULAR MICROBIOLOGY
(2016)
Article
Multidisciplinary Sciences
Rohitashw Kumar, Christine Breindel, Darpan Saraswat, Paul J. Cullen, Mira Edgerton
SCIENTIFIC REPORTS
(2017)
Article
Microbiology
Swetha Tati, Rui Li, Sumant Puri, Rohitashw Kumar, Peter Davidow, Mira Edgerton
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2014)
Article
Multidisciplinary Sciences
Sumant Puri, Rohitashw Kumar, Sonia Chadha, Swetha Tati, Heather R. Conti, Bernhard Hube, Paul J. Cullen, Mira Edgerton
Article
Multidisciplinary Sciences
Swetha Tati, Woong Sik Jang, Rui Li, Rohitashw Kumar, Sumant Puri, Mira Edgerton
Article
Microbiology
Andrew D. McCall, Rohitashw Kumar, Mira Edgerton
Article
Microbiology
Sumant Puri, Rohitashw Kumar, Isolde G. Rojas, Ornella Salvatori, Mira Edgerton
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2019)
Article
Microbiology
Rohitashw Kumar, Malabika Maulik, Ruvini U. Pathirana, Paul J. Cullen, Mira Edgerton
Article
Microbiology
Ornella Salvatori, Rohitashw Kumar, Sarah Metcalfe, Margaret Vickerman, Jason G. Kay, Mira Edgerton
Article
Microbiology
Hannah L. Norris, Rohitashw Kumar, Chih Yean Ong, Ding Xu, Mira Edgerton
Article
Microbiology
Hannah L. Norris, Rohitashw Kumar, Mira Edgerton
Summary: The study revealed that Hst 5+Zn-treated C. albicans cells show increased levels of cell-wall chitin, mannan, and beta-1,3 glucan, higher adhesion but lower invasiveness to oral epithelial cells, and promote anti-inflammatory cytokine release.
Article
Immunology
Rohitashw Kumar, Isolde Gina Rojas, Mira Edgerton
Summary: Candida albicans Sap6, a secreted aspartyl protease, activates immune cells through its protease activity and binding to host integrins. It has been found that Sap6 can interact with and activate human oral epithelial cells (OECs) through the PAR2 signaling pathway and its RGD domain. The study provides new insights into the mechanisms of Candida albicans oral infection and suggests potential drug targets for intervention.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Microbiology
Sumant Puri, Justin Friedman, Darpan Saraswat, Rohitashw Kumar, Rui Li, Donna Ruszaj, Mira Edgerton