4.7 Letter

Antimicrobial Activity of CHIR-090, an Inhibitor of Lipopolysaccharide Biosynthesis, against the Burkholderia cepacia Complex

期刊

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
卷 54, 期 8, 页码 3531-3533

出版社

AMER SOC MICROBIOLOGY
DOI: 10.1128/AAC.01600-09

关键词

-

资金

  1. NIGMS NIH HHS [R01 GM051310, GM-51310] Funding Source: Medline

向作者/读者索取更多资源

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.7
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

Article Biochemistry & Molecular Biology

Characterization of homologous sphingosine-1-phosphate lyase isoforms in the bacterial pathogen Burkholderia pseudomallei

Christopher J. McLean, Jon Marles-Wright, Rafael Custodio, Jonathan Lowther, Amanda J. Kennedy, Jacob Pollock, David J. Clarke, Alan R. Brown, Dominic J. Campopiano

JOURNAL OF LIPID RESEARCH (2017)

Review Biochemistry & Molecular Biology

Sphingolipid biosynthesis in man and microbes

Peter J. Harrison, Teresa M. Dunn, Dominic J. Campopiano

NATURAL PRODUCT REPORTS (2018)

Article Chemistry, Organic

The carbon chain-selective adenylation enzyme TamA: the missing link between fatty acid and pyrrole natural product biosynthesis

Piera M. Marchetti, Van Kelly, Joanna P. Simpson, Mairi Ward, Dominic J. Campopiano

ORGANIC & BIOMOLECULAR CHEMISTRY (2018)

Article Biochemistry & Molecular Biology

Solution Structure and Conformational Dynamics of a Doublet Acyl Carrier Protein from Prodigiosin Biosynthesis

Thitapa Thongkawphueak, Ashley J. Winter, Christopher Williams, Hannah J. Maple, Siriwat Soontaranon, Chonthicha Kaewhan, Dominic J. Campopiano, Matthew P. Crump, Pakorn Wattana-Amorn

Summary: This study presents the NMR structure analysis of the doublet ACP domains from PigH, revealing the inherent plasticity of PigH ACP(1)-ACP(2) structure with conformational exchange predominantly in the ACP2 domain. Furthermore, ensemble models obtained from SAXS data show distinct conformers, bent and extended, of both apo and holo PigH ACP(1)-ACP(2) mediated by the central linker. These results provide new insights into the behavior of the interdomain linker of multiple ACP domains in modulating protein-protein interactions, which may have implications for metabolic engineering in prodigiosin and related biosynthetic pathways.

BIOCHEMISTRY (2021)

Article Biochemistry & Molecular Biology

Creation of an Engineered Amide Synthetase Biocatalyst by the Rational Separation of a Two-Step Nitrile Synthetase

Alexis J. A. Hennessy, Wenli Huang, Chloe Savary, Dominic J. Campopiano

Summary: The synthesis of amides through acid and amine coupling is common but requires more sustainable and biocatalytic methods. The study identified the potential of nitrile synthetase in amide bond formation and successfully engineered a biocatalyst without the need for coupling agents. This suggests the further development of nitrile synthetases as efficient, coupling agent-free biocatalysts for amide formation.

CHEMBIOCHEM (2022)

Article Chemistry, Multidisciplinary

N-Phenylputrescine (NPP): a natural product inspired amine donor for biocatalysis

Catherine A. McKenna, Maria Stiblarikova, Irene De Silvestro, Dominic J. Campopiano, Andrew L. Lawrence

Summary: The synthesis of chiral amines is a key reaction in chemical synthesis. Researchers are striving to develop more sustainable alternatives to metal-catalyzed methods currently used in various industries. By using N-phenylputrescine as an amine donor, a broad range of synthetically useful TA biocatalysts can be utilized under various reaction conditions, demonstrating its potential in practical applications like synthesizing pharmaceuticals such as sitagliptin.

GREEN CHEMISTRY (2022)

Article Biochemistry & Molecular Biology

BioWF: A Naturally-Fused, Di-Domain Biocatalyst from Biotin Biosynthesis Displays an Unexpectedly Broad Substrate Scope

Shona M. Richardson, Peter J. Harrison, Michael A. Herrera, Menglu Wang, Rebecca Verez, Gustavo Perez Ortiz, Dominic J. Campopiano

Summary: The carbon backbone of biotin is synthesized from the C-7 diacid pimelate, and the conversion to an acyl-CoA thioester is catalyzed by an ATP-dependent pimeloyl-CoA synthetase (PCAS, encoded by BioW). This acyl-thioester is then condensed with β-alanine to form an aminoketone. The study found that the BioW-BioF fusion from Corynebacterium amycolatum accepts a wider range of substrates, suggesting it is a versatile biocatalyst.

CHEMBIOCHEM (2022)

Article Microbiology

Characterization of inositol lipid metabolism in gut-associated Bacteroidetes

Stacey L. Heaver, Henry H. Le, Peijun Tang, Arnaud Basle, Claudia Mirretta Barone, Dai Long Vu, Jillian L. Waters, Jon Marles-Wright, Elizabeth L. Johnson, Dominic J. Campopiano, Ruth E. Ley

Summary: This study characterizes the pathways responsible for inositol lipid production in human gut Bacteroides and explores the importance of these lipids for capsule expression, antimicrobial peptide resistance, and colonization. The authors identify the biosynthetic pathway and enzymes involved in inositol lipid synthesis, and show that loss of inositol lipid production affects bacterial fitness and symbiosis in a mouse model. They also discover a previously undescribed pathway for bacterial PI-DAG synthesis. Overall, the findings suggest that inositol sphingolipid production is widespread in host-associated Bacteroidetes and has implications for symbiotic relationships.

NATURE MICROBIOLOGY (2022)

Article Chemistry, Physical

Coupled Natural Fusion Enzymes in a Novel Biocatalytic Cascade Convert Fatty Acids to Amines

Shona M. Richardson, Piera M. Marchetti, Michael A. Herrera, Dominic J. Campopiano

Summary: A unique di-domain biocatalyst (PtTamH) has been discovered, which can convert fatty acids to amines and has potential applications. Moreover, the structural organization of PtTamH has been revealed.

ACS CATALYSIS (2022)

Article Chemistry, Analytical

An improved, optimised and robust keratin azure assay for accurate assessment of keratinase activity

Rhona M. Cowan, Eleanor Birch, Grace Nisbet, Chimaeze Onyeiwu, Clare Campbell, Ian Archer, Dominic J. Campopiano

Summary: This study introduces a novel method for identifying and quantifying keratinase activity, which can be used for valorising the alpha-keratin found in natural wool fibres.

ANALYTICAL METHODS (2023)

Article Chemistry, Organic

Direct monitoring of biocatalytic deacetylation of amino acid substrates by 1H NMR reveals fine details of substrate specificity

Silvia De Cesare, Catherine A. McKenna, Nicholas Mulholland, Lorna Murray, Juraj Bella, Dominic J. Campopiano

Summary: In this study, the l-selective ornithine deacetylase ArgE was shown to catalyze the hydrolysis of a wide range of N-acyl-amino acid substrates. The appearance of the acetate product signal was monitored using H-1 NMR spectroscopy, revealing the enzyme's activity. This method was also utilized to investigate the subtle structural selectivity of the biocatalyst with a substrate capable of adopting different rotameric conformations.

ORGANIC & BIOMOLECULAR CHEMISTRY (2021)

Article Chemistry, Physical

d-Phenylglycine aminotransferase (d-PhgAT) - substrate scope and structural insights of a stereo-inverting biocatalyst used in the preparation of aromatic amino acids

Annabel Serpico, Silvia De Cesare, Jon Marles-Wright, M. Kalim Akhtar, Gary J. Loake, Dominic J. Campopiano

CATALYSIS SCIENCE & TECHNOLOGY (2020)

Article Biochemistry & Molecular Biology

Synthesis of N-acyl amide natural products using a versatile adenylating biocatalyst

Piera M. Marchetti, Shona M. Richardson, Noor M. Kariem, Dominic J. Campopiano

MEDCHEMCOMM (2019)

Article Chemistry, Organic

Non-invasive 19F NMR analysis of a protein-templated N-acylhydrazone dynamic combinatorial library

Alexander G. Ekstrom, Jue Theresa Wang, Juraj Bella, Dominic J. Campopiano

ORGANIC & BIOMOLECULAR CHEMISTRY (2018)

Article Biotechnology & Applied Microbiology

Hydrogen Peroxide-Based Fluorometric Assay for Real-Time Monitoring of SAM-Dependent Methyltransferases

M. Kalim Akhtar, Dhanya Vijay, Saima Umbreen, Chris J. McLean, Yizhi Cai, Dominic J. Campopiano, Gary J. Loake

FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY (2018)

暂无数据