Article
Multidisciplinary Sciences
Xin Hu, Shuangying Jiang, Feifei Xu, Cheng Zeng, Xiangxiang Wang, Wei Liu, Aimin Cheng, Chengying Ma, Ning Gao, Yuyu Zhao, Junbiao Dai, Guanghou Zhao
Summary: Emerging evidence suggests that ribosomes are dynamic machines with heterogeneous protein compositions, and their abilities to translate and adapt to environmental changes can be reshaped. Duplications of ribosomal protein genes affect cell function through gene dose amplification and paralog-specific regulation, providing robustness and phenotypic plasticity.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Immunology
Bernard Ong'ondo Osero, Zama Cele, Raphael Taiwo Aruleba, Rebeng A. Maine, Mumin Ozturk, Manfred B. Lutz, Frank Brombacher, Ramona Hurdayal
Summary: This study suggests that IL-4R alpha signaling on DCs is not key in the regulation of immune-mediated protection in mice against L. mexicana infection.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Microbiology
Sarah Hendrickx, Joao Luis Reis-Cunha, Sarah Forrester, Daniel C. Jeffares, Guy Caljon
Summary: The study identified potential genetic variations associated with paromomycin resistance in Leishmania, including genes involved in transcription, translation, protein turnover, virulence, mitochondrial function, signaling, and vesicular trafficking. These results suggest that paromomycin affects protein translational processes and highlights the complex and multifactorial origin of resistance in Leishmania.
Article
Immunology
Alma Reyna Escalona-Montano, Mariana Zuniga-Fabian, Nallely Cabrera, Ricardo Mondragon-Flores, Jenny Nancy Gomez-Sandoval, Araceli Rojas-Bernabe, Augusto Gonzalez-Canto, Laila Gutierrez-Kobeh, Ruy Perez-Montfort, Ingeborg Becker, Maria Magdalena Aguirre-Garcia
Summary: In this study, the researchers cloned and characterized the structure and function of PP2C in Leishmania, finding that LmxPP2C exhibited protein dephosphorylation activity under specific conditions. Immunodetection showed its localization in the flagellar structures of the protozoan, suggesting a potentially important role in specific cellular processes.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Aditya M. Kunjapur, Michael G. Napolitano, Eriona Hysolli, Karen Noguera, Evan M. Appleton, Max G. Schubert, Michaela A. Jones, Siddharth Iyer, Daniel J. Mandell, George M. Church
Summary: Research demonstrates that synthetic auxotrophy shows stability in continuous evolution, enabling normal growth at low concentrations, with potential applications for microbial containment. Allelic reconstruction reveals the contribution of three genes to fitness at low BipA concentrations. Introduction of the progenitor strain into mammalian cell culture shows effective containment of bacteria.
Article
Biochemistry & Molecular Biology
Timur N. Baymukhametov, Dmitry N. Lyabin, Yury M. Chesnokov, Ivan I. Sorokin, Evgeniya Pechnikova, Alexander L. Vasiliev, Zhanna A. Afonina
Summary: Polyribosomes, groups of ribosomes simultaneously translating a single mRNA molecule, are common in both prokaryotic and eukaryotic cells. Recent studies have shown that circular polyribosomes, although not abundant, have pseudo-regular structures and can enhance translation.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Multidisciplinary Sciences
Suad Gazi Jaafer Husaine Al Kufi, Josiah Emmerson, Heidi Rosenqvist, Catarina Mateus Moreira Garcia, Diana Onodelia Rios-Szwed, Martin Wiese
Summary: Kinesins, including LmxKIN29, play important roles in cell division, intracellular organization, and flagellum formation and maintenance. The study suggests that LmxKIN29 is a potential drug target for leishmaniasis based on its crucial role in the disease and its divergence from mammalian kinesins.
SCIENTIFIC REPORTS
(2022)
Article
Immunology
Lenka Pacakova, Karel Harant, Petr Volf, Tereza Lestinova
Summary: This study compared the proteome of Leishmania mexicana amastigotes from lesion of infected BALB/c mice with alternately cultivated amastigotes. Significant differences in the abundance of metabolic enzymes, virulence factors and proteins involved in translation and condensation of DNA were observed, with axenic amastigotes showing the most pronounced differences compared to lesion-derived amastigotes, and macrophage-derived amastigotes mostly intermediate between the two.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Review
Chemistry, Multidisciplinary
Xuejiao Zhang, Ievgen S. Donskyi, Weihao Tang, Shuo Deng, Daxu Liu, Siyu Zhang, Qing Zhao, Baoshan Xing
Summary: The remarkable progress in applied black phosphorus nanomaterials is due to its outstanding properties. However, considering the potential environmental release and human exposure, it is important to understand how these nanomaterials impact biological systems and human health.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Biochemistry & Molecular Biology
Adullah Alotaibi, Godwin U. Ebiloma, Roderick Williams, Ibrahim A. Alfayez, Manal J. Natto, Sameah Alenezi, Weam Siheri, Malik AlQarni, John O. Igoli, James Fearnley, Harry P. De Koning, David G. Watson
Summary: Ethanolic extracts of temperate zone propolis from the UK and Poland exhibited anti-trypanosomal activity, with compounds like naringenin 4 ',7-dimethyl ether and 4 '-methoxykaempferol showing the highest activity. Different compounds were isolated and characterized, showing varying levels of activity against drug-resistant strains of T. brucei and L. mexicana, without a clear structure-activity relationship observed. Multiple compounds from temperate propolis have anti-kinetoplastid activity.
Article
Microbiology
Exequiel O. Porta, Liqian Gao, Paul W. Denny, Patrick G. Steel, Karunakaran Kalesh
Summary: This study systematically characterizes the perturbing effect of HSP90 inhibition on the phosphoproteome of Leishmania mexicana, revealing distinct molecular effects in different life cycle stages. The findings provide insight into selective molecular targeting in the therapeutically relevant amastigote stage.
MICROBIOLOGY SPECTRUM
(2023)
Article
Parasitology
Elizabeth M. Coser, Bianca A. Ferreira, Edite H. Yamashiro-Kanashiro, Jose Angelo L. Lindoso, Adriano C. Coelho
Summary: Treatment of tegumentary leishmaniasis in Brazil is currently limited to a few drugs, highlighting the need for expansion of therapeutic options. Paromomycin, an aminoglycoside antibiotic already approved for visceral leishmaniasis, shows promising activity against clinical isolates of tegumentary leishmaniasis in Brazil.
Article
Biochemistry & Molecular Biology
Cristina Remes, Anas Khawaja, Sarah F. Pearce, Adam M. Dinan, Shreekara Gopalakrishna, Miriam Cipullo, Vasileios Kyriakidis, Jingdian Zhang, Xaquin Castro Dopico, Olessya Yukhnovets, Ilian Atanassov, Andrew E. Firth, Barry Cooperman, Joanna Rorbach
Summary: The detailed mechanism of mitochondrial translation in human mitochondria remains unclear. This study reveals that leaderless mRNA transcripts can be directly loaded onto assembled 55S mitochondrial ribosomes in human mitochondria, but not onto the small subunit, and this process requires initiator fMet-tRNA(Met) binding. Furthermore, this study demonstrates that mtIF3 is not involved in translation of leaderless mitochondrial transcripts but is essential for translation of ATP6 in the bicistronic ATP8/ATP6 transcript. These findings provide insights into the evolutionary divergence of mitochondrial translation and enhance our understanding of a critical process in eukaryotic metabolism.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Parasitology
Nadia Soto-Olguin, Jaime Zamora-Chimal, Jose Delgado-Dominguez, Ingeborg Becker
Summary: The activation of γδ T cells through TLR2 by Leishmania mexicana LPG was investigated. The study found that LPG activates TLR2 and leads to the activation of γδ T cells. Other receptors were also found to recognize LPG and contribute to the increase in γδ T cell numbers.
ACTA PARASITOLOGICA
(2023)
Article
Parasitology
Bianca A. Ferreira, Thaynan F. C. Martins, Elizabeth M. Coser, Viviane da L. Oliveira, Edite H. Yamashiro-Kanashiro, Mussya C. Rocha, Marcelo M. Pinto, Paulo C. Cotrim, Adriano C. Coelho
Summary: The study confirmed the endemicity of canine leishmaniasis in Embu das Artes, Brazil, and found that isolates from this region are susceptible to paromomycin and miltefosine, indicating the potential of these drugs for the clinical treatment of human visceral leishmaniasis in Brazil.
PARASITOLOGY RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Maria F. Beer, Augusto E. Bivona, Andres Sanchez Alberti, Natacha Cerny, Guillermo F. Reta, Victor S. Martin, Jose M. Padron, Emilio L. Malchiodi, Valeria P. Suelsen, Osvaldo J. Donadel
Article
Biochemistry & Molecular Biology
Valeria P. Sulsen, Emilio F. Lizarraga, Orlando G. Elso, Natacha Cerny, Andres Sanchez Alberti, Augusto E. Bivona, Emilio L. Malchiodi, Silvia I. Cazorla, Cesar A. N. Catalan
Article
Biochemistry & Molecular Biology
Andrea A. E. Mendez, Liliana B. Pena, Lucrecia M. Curto, Marisa M. Fernandez, Emilio L. Malchiodi, Sara M. Garza-Aguilar, Jorge M. Vazquez-Ramos, Susana M. Gallego
Article
Immunology
Sofia Noli Truant, Mauricio C. De Marzi, Maria B. Sarratea, Maria B. Antonoglou, Ana P. Meo, Laura V. Iannantuono Lopez, Maria J. Fernandez Lynch, Marcos Todone, Emilio L. Malchiodi, Marisa M. Fernandez
FRONTIERS IN IMMUNOLOGY
(2020)
Article
Pathology
Pablo Cassaglia, Federico Penas, Celeste Betazza, Florencia Fontana Estevez, Veronica Miksztowicz, Nadia Martinez Naya, Maria Clara Llamosas, Sofia Noli Truant, Luciana Wilensky, Veronica Volberg, Agata C. Cevey, Vanessa Touceda, Eliana Cicale, Gabriela Berg, Marisa Fernandez, Nora Goren, Celina Morales, German E. Gonzalez
AMERICAN JOURNAL OF PATHOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Orlando G. Elso, Augusto E. Bivona, Andres Sanchez Alberti, Natacha Cerny, Lucas Fabian, Celina Morales, Cesar A. N. Catalan, Emilio L. Malchiodi, Silvia Cazorla, Valeria P. Sulsen
Article
Multidisciplinary Sciences
Julia Lara, Lautaro Diacovich, Felipe Trajtenberg, Nicole Larrieux, Emilio L. Malchiodi, Marisa M. Fernandez, Gabriela Gago, Hugo Gramajo, Alejandro Buschiazzo
NATURE COMMUNICATIONS
(2020)
Article
Immunology
Maria Belen Antonoglou, Andres Sanchez Alberti, Daniela Maria Redolfi, Augusto Ernesto Bivona, Maria Julieta Fernandez Lynch, Sofia Noli Truant, Maria Belen Sarratea, Laura Valeria Iannantuono Lopez, Emilio Luis Malchiodi, Marisa Mariel Fernandez
FRONTIERS IN IMMUNOLOGY
(2020)
Article
Parasitology
Maria Priscila Saracino, Cecilia Celeste Vila, Melina Cohen, Maria Virginia Gentilini, Guido Hernan Falduto, Marcela Adriana Calcagno, Estela Roux, Stella Maris Venturiello, Emilio Luis Malchiodi
PARASITES & VECTORS
(2020)
Article
Biochemistry & Molecular Biology
Sarratea Maria Belen, Noli Truant Sofia, Mitarotonda Romina, Antonoglou Maria Belen, Chiappini Santiago, Fernandez Lynch Maria Julieta, Romasanta Pablo, Vescina Cristina, Desimone Martin, De Marzi Mauricio, Malchiodi Emilio, Fernandez Marisa
Summary: Staphylococcal enterotoxins are important causative agents of food poisoning, functioning as both gastrointestinal toxins and superantigens. Symptoms include vomiting and diarrhea, with potential severe cases leading to toxic shock syndrome. The use of SPR technology and silica nanoparticles for signal amplification has shown to enhance the detection sensitivity of SAgs.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Chemistry, Multidisciplinary
Andres Sanchez Alberti, Maria F. Beer, Natacha Cerny, Augusto E. Bivona, Lucas Fabian, Celina Morales, Albertina Moglioni, Emilio L. Malchiodi, Osvaldo J. Donadel, Valeria P. Sulsen
Summary: The study demonstrated that cumanin diacetate showed promising activity and selectivity against Trypanosoma cruzi, especially in inhibiting parasite replication in amastigote forms. This compound could be a potential candidate for further studies in the development of new therapies against Chagas disease.
Review
Food Science & Technology
Sofia Noli Truant, Daniela Maria Redolfi, Maria Belen Sarratea, Emilio Luis Malchiodi, Marisa Mariel Fernandez
Summary: Staphylococcal enterotoxins are a diverse family of bacterial exotoxins that can activate a large proportion of the host T cells. However, these toxins exhibit significant heterogeneity in their structure and homology. The mechanisms underlying their production are not fully understood. Nonetheless, studying these superantigens is crucial for understanding their functions, pathogenicity, and potential for immunotherapeutic interventions.
Article
Pharmacology & Pharmacy
Jimena Borgo, Orlando G. Elso, Jessica Gomez, Mauro Coll, Cesar A. N. Catalan, Juan Mucci, Guzman Alvarez, Lia M. Randall, Patricia Barrera, Emilio L. Malchiodi, Augusto E. Bivona, Maria Florencia Martini, Valeria P. Suelsen
Summary: Stevia species, particularly S. satureiifolia var. satureiifolia and S. alpina, are rich in terpenoid compounds with antiprotozoal activity. Two sesquiterpene lactones, santhemoidin C and 2-oxo-8-deoxyligustrin, were isolated and evaluated for their trypanocidal activity against Trypanosoma cruzi. Santhemoidin C showed promising inhibitory activity against the parasite's different stages and the Tc80 protein, making it a potential candidate for the development of trypanocidal drugs.