Article
Chemistry, Applied
Yingying Li, Yingying Zhang, Chaodi Kang, Wentao Zhao, Shilei Li, Shouwei Wang
Summary: Food fraud is a global issue that has raised wide public concern in recent years. Mass spectrometry techniques have a significant advantage in analyzing food authenticity. A simple, specific, and fast LC-MS/MS method has been developed to quantitatively determine pork content in processed meat products.
Article
Nutrition & Dietetics
Tobias Messmer, Richard G. J. Dohmen, Lieke Schaeken, Lea Melzener, Rui Hueber, Mary Godec, Carin Didoss, Mark J. Post, Joshua E. Flack
Summary: The study characterized cellular heterogeneity within bovine muscle and muscle-derived cell cultures over time using single-cell RNA sequencing. The researchers identified numerous distinct cell types and developed protocols for easy purification and proliferation of these populations. They also used transcriptomics to identify conditions that promote the growth of specific cell types, providing insights for efficient cultured meat production.
FRONTIERS IN NUTRITION
(2023)
Article
Chemistry, Analytical
Youli Tian, Yiren Cao, Genhan Zha, Ke-Er Chen, Muhammad Idrees Khan, Jicun Ren, Weiwen Liu, Yuxing Wang, Qiang Zhang, Chengxi Cao
Summary: This study proposes a marker-free isoelectric focusing method that uses a convolutional neural network model to efficiently and accurately classify isoelectric focusing patterns. A dataset of 1449 isoelectric focusing patterns was collected and experimental pH gradient drift was introduced to enhance the model's robustness. With simple preprocessing, rapid inference speed (only 50 ms), and a high accuracy of 97.1%, the CNN model outperformed traditional algorithms in simultaneously identifying meat species and cuts, indicating its great potential for large-scale isoelectric focusing analyses of complex protein samples when combined with microstrip isoelectric focusing.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Jia Li, Quanhu Sheng, Yu Shyr, Qi Liu
Summary: Single-cell RNA sequencing is a powerful method for identifying and characterizing cellular heterogeneity. This study introduces a bidirectional method, scMRMA, which utilizes a hierarchical reference to improve annotation accuracy. scMRMA outperforms existing methods in multiple benchmark datasets and successfully reveals CD8 T cell population expansion in squamous cell carcinoma after anti-PD-1 treatment.
NUCLEIC ACIDS RESEARCH
(2022)
Review
Food Science & Technology
Ee Theng Ng, Satnam Singh, Wee Swan Yap, Shi Hua Tay, Deepak Choudhury
Summary: Cultured meat has emerged as a breakthrough technology with the potential to minimize negative impacts associated with population growth. A patentometric analysis was conducted to highlight the current patent landscape in the field, identifying active organizations and geographical locations. The analysis of over 190 patents published in English from 1997 to 2020 provided valuable insights into the technology focus, origin country, and assignees.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Article
Food Science & Technology
Christof Falkenberg, Alena Trexler, Christian Garaus, Siegfried Poechtrager
Summary: The consumption of meat substitutes has grown significantly in the past decade. In order to understand the potential of plant-based meat alternatives, it is crucial to have detailed knowledge of the current market offerings. This study analyzed plant-based minced and sausage products in Austrian supermarkets, comparing them to conventional meat and incorporating results from a comparative study in Australia. The findings highlight the potential of meat substitutes as an alternative protein source, as they have comparable protein content but significantly lower caloric intake. However, plant-based products are still priced significantly higher than conventional meat.
Article
Food Science & Technology
Mirian dos Santos, Daniela Almeida Vieira Fogaca da Rocha, Oigres Daniel Bernardinelli, Fernando Divino Oliveira Junior, Diogenes Gomes de Sousa, Edvaldo Sabadini, Rosiane Lopes da Cunha, Marco Antonio Trindade, Marise Aparecida Rodrigues Pollonio
Summary: Hybrid meat products are a great way to incorporate plant proteins into traditional meat formulations. Soy, pea, and sunflower proteins are compatible with the meat matrix, resulting in stable emulsion systems, cohesive protein networks, and good fat distribution. However, rice and fava bean proteins lead to poor emulsion stability.
Review
Agriculture, Dairy & Animal Science
Jose Felipe Orzuna-Orzuna, Griselda Dorantes-Iturbide, Alejandro Lara-Bueno, German David Mendoza-Martinez, Luis Alberto Miranda-Romero, Hector Aaron Lee-Rangel
Summary: The addition of tannins in sheep diets improves productive performance, antioxidant status in blood serum, oxidative stability of meat and some other characteristics related to meat and carcass quality. Tannin supplementation has positive effects on growth and antioxidant capacity in sheep, as well as improving meat quality and shelf life.
Review
Food Science & Technology
Urska Dobersek, Kelsey Teel, Sydney Altmeyer, Joshua Adkins, Gabrielle Wy, Jackson Peak
Summary: This meta-analysis examined the relationship between meat consumption or avoidance, depression, and anxiety. The findings showed that meat consumption was associated with lower levels of depression and anxiety compared to abstaining from meat. Meat consumers also experienced lower levels of depression compared to vegans. The quality of the studies explained a significant portion of the heterogeneity in depression and anxiety. The analysis suggests that higher quality studies show a more positive and consistent relationship between meat consumption and better mental health.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Article
Agriculture, Multidisciplinary
Vaikundamoorthy Ramalingam, Inho Hwang
Summary: The study compared the transcriptomic profiles of fibroblasts from different muscles, revealing genes associated with factors driving meat quality like collagen synthesis, cell differentiation, and muscle tissue development. Networks showed a close relationship between intramuscular fat deposition and meat tenderness, with real-time PCR validation confirming key roles in muscle growth and tenderness. The analysis highlighted various genes responsible for toughness and tenderness variance in different beef muscles.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Environmental Sciences
Guangxing Wang, Zhihao Yin, Zhigang Hu, Gang Chen, Wei Li, Yadong Bo
Summary: The study analyzed the performance of the BeiDou Global Ionospheric Model (BDGIM) through Single Point Positioning (SPP) experiments, and found that it performs best in high-latitude areas, significantly improving positioning accuracy. Accuracy in the northern hemisphere is better than in the southern hemisphere, with a global average positioning accuracy of 4.60 meters, and there are seasonal differences in performance.
Article
Engineering, Electrical & Electronic
Davide Magrin, Martina Capuzzo, Andrea Zanella, Michele Zorzi
Summary: This paper presents a mathematical model for estimating the performance of a LoRaWAN gateway serving a set of devices. The model allows for systematic analysis of different configurations and is validated through simulations.
IEEE TRANSACTIONS ON WIRELESS COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Xueqin Cao, Muchun Li, Liuchun Ma, Miaomiao Wang, Xueling Hou, Maitinuer Maiwulanjiang
Summary: In this study, the chromatographic fingerprint of Artemisia rupestris L. samples was established, and the constituents of ten components were determined using quantitative analysis of multi-components by single marker (QAMS) and the external standard method (ESM). The results showed that the QAMS method and chromatographic fingerprint analysis were feasible and accurate in evaluating the quality of Artemisia rupestris L., making them a reliable reference for traditional Chinese medicine quality control.
Article
Agriculture, Dairy & Animal Science
Piotr Matyba, Tomasz Florowski, Krzysztof Dasiewicz, Karolina Ferenc, Jaroslaw Olszewski, Michal Trela, Gilbert Galemba, Miroslaw Slowinski, Maria Sady, Dominika Domanska, Zdzislaw Gajewski, Romuald Zabielski
Summary: Pigs with intrauterine growth restriction (IUGR) have high perinatal mortality and dysfunction of internal organs, adipose, and muscle tissues. This study compares IUGR pigs and normal pigs from birth to slaughter, finding that although IUGR pigs have lower slaughter weight, their meat quality is not significantly different from normal pigs and is even preferred by consumers for its taste, smell, and tenderness. The slightly different chemical composition and structure of muscle tissue in IUGR pigs contribute to their consumer appeal.
Article
Multidisciplinary Sciences
Monica T. Dayao, Maigan Brusko, Clive Wasserfall, Ziv Bar-Joseph
Summary: RAMCES is a method that utilizes a convolutional neural network to learn optimal markers and perform cell segmentation. Testing on existing datasets demonstrates its ability to accurately identify cell boundary markers and assign cell types in new spatial proteomics data.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Siriluck Ponsuksili, Michael Oster, Henry Reyer, Frieder Hadlich, Nares Trakooljul, Markus Rodehutscord, Amelia Camarinha-Silva, Joern Bennewitz, Klaus Wimmers
Summary: This study identified miRNAs and mRNAs related to phosphorus utilization in Japanese quail, along with their genetic regulation and association with microbiota. The results provide novel insights into the roles of mRNA and miRNA in host gut tissue functions.
Article
Genetics & Heredity
Marua Abu Risha, Puntita Siengdee, Dirk Dannenberger, Klaus Wimmers, Siriluck Ponsuksili
Summary: The study found that PUFAs have an impact on the transition of C2C12 cells from proliferation to differentiation phase, prolonging proliferation and preventing differentiation.
Article
Genetics & Heredity
Frieder Hadlich, Henry Reyer, Michael Oster, Nares Trakooljul, Eduard Murani, Siriluck Ponsuksili, Klaus Wimmers
Summary: Commercial and customized microarrays are valuable tools for analyzing holistic expression patterns, but require integration of the latest genomic information. This study presents a comprehensive workflow using the rePROBE R package to assign probes and annotate probe sets based on up-to-date genomic and transcriptomic data. The workflow reveals updated probe assignments and annotations for various livestock species and human, with a focus on providing a detailed basis for comprehensive analyses such as eQTL studies.
GENOMICS PROTEOMICS & BIOINFORMATICS
(2021)
Article
Spectroscopy
Kay Sowoidnich, Michael Oster, Klaus Wimmers, Martin Maiwald, Bernd Sumpf
Summary: The study demonstrates the potential of shifted excitation Raman difference spectroscopy (SERDS) for inspecting intact feed pellets and their constituents, with the ability to efficiently analyze various components and promising prospects for future development.
JOURNAL OF RAMAN SPECTROSCOPY
(2021)
Article
Agronomy
Dario Fornara, Elizabeth M. E. Ball, Christina Mulvenna, Henry Reyer, Michael Oster, Klaus Wimmers, Hanne Damgaard Poulsen, Arno Rosemarin
Summary: The over-supplementation of animal feeds with phosphorus leads to high rates of P excretion and negative environmental impacts. Adding phytase to pig and poultry diets can reduce P excretion, but the effects on plant-soil systems are poorly understood. This study found that phytase supplementation significantly influenced P content of animal waste and plant P dynamics, with intermediate phytase levels contributing to higher P use efficiency and lower soil P availability. The effects of dietary P content on P losses from soils varied depending on the type of organic amendment.
Article
Agriculture, Dairy & Animal Science
Christina C. Mulvenna, Ursula M. McCormack, Elizabeth Magowan, John McKillen, Mike R. Bedford, Carrie L. Walk, Michael Oster, Henry Reyer, Klaus Wimmers, Dario A. Fornara, M. Elizabeth E. Ball
Summary: Phytase has been a common addition to poultry diets for over 20 years, showing benefits in growth performance, nutrient digestibility, and bone parameters. This study found that superdosing phytase in rapeseed meal and maize dried distiller grains with solubles diets can improve performance, increase digestibility, and reduce P excretion by 15% compared to birds without phytase.
Article
Biochemistry & Molecular Biology
Maruf Hasan, Michael Oster, Henry Reyer, Siriluck Ponsuksili, Eduard Murani, Petra Wolf, Dagmar-Christiane Fischer, Klaus Wimmers
Summary: This study investigated the tissue-wide expression patterns of key genes related to vitamin D metabolism and FGF23 signaling in pigs fed different phosphorus diets. The results showed that non-renal tissues and cells have specific requirements for active vitamin D formation. Low phosphorus diet suppressed vitamin D signaling locally, while high phosphorus diet stimulated local vitamin D synthesis and regulation of FGF23 signaling in bone.
Article
Agriculture, Dairy & Animal Science
Adewunmi O. Omotoso, Henry Reyer, Michael Oster, Steffen Maak, Siriluck Ponsuksili, Klaus Wimmers
Summary: Phosphorus inclusion in broiler diets needs to be tailored to their physiological demands at different developmental stages. A timed nutritional conditioning strategy, such as P depletion, may enhance mineral homeostasis mechanisms and reduce dietary P supply. This study investigated the effects of dietary P depletion on broiler chickens at different developmental stages, revealing marked responses early on, but indications of compensatory mechanisms later. Endocrine control mediated by calcitriol, intestinal P uptake, and mineral mobilization from bones were identified as potential mechanisms for maintaining mineral homeostasis. Precise timing, duration, and extent of P depletion should be considered for optimized nutrient utilization in broiler husbandry.
Article
Chemistry, Analytical
Mohd. Rahil Hasan, Pradakshina Sharma, Shifa Shaikh, Saumitra Singh, Roberto Pilloton, Jagriti Narang
Summary: We have developed a paper-based experimental platform for analyzing dengue virus, which incorporates screen-printing technology and black carbon conductive ink. The platform utilizes both two-electrode and three-electrode systems, with an LOD of 0.1 μg/mL for detecting the virus. The three-electrode system exhibits a significantly higher current range and amplification compared to the two-electrode system, making it a viable option for sensitive detection of dengue virus and other diseases.
Article
Nutrition & Dietetics
Maruf Hasan, Michael Oster, Henry Reyer, Klaus Wimmers, Dagmar-Christiane Fischer
Summary: Vitamin D-3 (Vit D-3) and 25(OH)D-3 are used as dietary sources of active vitamin D in pig husbandry, and while their primary effects are on the intestine, kidney, and bone, they also have wide-ranging effects on peripheral tissues. The use of Vit D-3 and 25(OH)D-3 in pig nutrition has been shown to have different outcomes in terms of reproductive capacities, growth performance, immunity, and bone development.
BRITISH JOURNAL OF NUTRITION
(2023)
Correction
Nutrition & Dietetics
Maruf Hasan, Michael Oster, Henry Reyer, Klaus Wimmers, Dagmar-Christiane Fischer
BRITISH JOURNAL OF NUTRITION
(2023)
Article
Agriculture, Dairy & Animal Science
Hanne Honerlagen, Henry Reyer, Ibrahim Abou-Soliman, Dierck Segelke, Siriluck Ponsuksili, Nares Trakooljul, Norbert Reinsch, Bjoern Kuhla, Klaus Wimmers
Summary: Improving the nitrogen-utilization efficiency (NUE) of dairy cows through breeding selection has benefits in terms of nutrition, environment, and economics. The individual milk urea concentration (MU) of cows has been proposed as an indicator trait for NUE. This study aimed to identify different microbial genera in the rumen of Holstein cows with divergent genomic breeding values for MU (GBVMU). The identified microbial genera were further investigated for their correlations with MU and other NUE-associated traits, providing insight into genetically determined N-utilization in Holstein cows.
JOURNAL OF DAIRY SCIENCE
(2023)
Article
Cell Biology
Julia Brenmoehl, Elli Brosig, Nares Trakooljul, Christina Walz, Daniela Ohde, Antonia Noce, Michael Walz, Martina Langhammer, Stefan Petkov, Monika Roentgen, Steffen Maak, Christina E. E. Galuska, Beate Fuchs, Bjoern Kuhla, Siriluck Ponsuksili, Klaus Wimmers, Andreas Hoeflich
Summary: This study investigated the contribution of non-mitochondrial and mitochondrial metabolic pathways in contracting muscles during repeated training. The results showed that marathon mice displayed increased glucose and fatty acid oxidation, reduced lactate dehydrogenase activity, and inhibited lactate formation compared to trained control mice. This suggests that marathon mice have enhanced metabolic flexibility towards lipid catabolism regulated by lactate dehydrogenase.
Review
Agriculture, Dairy & Animal Science
Benjamin Diehl, Michael Oster, Andreas Vernunft, Klaus Wimmers, Hartwig Bostedt
Summary: The losses of piglets in commercial pig farming remain a concern and sustainable breeding and management strategies are needed. This review systematically presents the three phases of neonatal adaptation in piglets and highlights the key developments and influencing factors in each phase.
ARCHIVES ANIMAL BREEDING
(2022)
Review
Biochemistry & Molecular Biology
Fabio Sarais, Alvaro Perdomo-Sabogal, Klaus Wimmers, Siriluck Ponsuksili
Summary: This review focuses on the latest discoveries of tsRNAs, with special attention to molecules initially thought to be associated with tiRNAs. The paper outlines their biological functions, provides a collection of useful databases, and discusses future research perspectives and applications in livestock basic and applied research.