Article
Genetics & Heredity
Kaitlyn R. Daza, Deborah Velez-Irizarry, Sebastian Casiro, Juan P. Steibel, Nancy E. Raney, Ronald O. Bates, Catherine W. Ernst
Summary: This study integrated miRNA and gene expression data to identify 23 miRNA expression Quantitative Trait Loci (miR-eQTL) in pigs, with miR-874 being associated with multiple production traits. The results provide insight into genomic regions underlying miRNA expression variation and potential regulatory effects on economically important traits in the global pig industry.
FRONTIERS IN GENETICS
(2021)
Article
Cell Biology
Baohua Tan, Sheng Wang, Shanshan Wang, Jiekang Zeng, Linjun Hong, Zicong Li, Jie Yang, Gengyuan Cai, Enqin Zheng, Zhenfang Wu, Ting Gu
Summary: This study provided a comprehensive genome-wide view of H3K27me3 during porcine embryonic muscle development, revealing its continuous increase and negative correlation with gene expression. It also showed that H3K27me3 could enrich in the promoter to negatively regulate gene expression and impact muscle cell differentiation in pigs.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
Rui Zhang, Christiane Neuhoff, Qin Yang, Mehmet U. Cinar, Muhammad J. Uddin, Ernst Tholen, Karl Schellander, Dawit Tesfaye
Summary: This study found that sulforaphane (SFN) can enhance the proliferation of porcine satellite cells (PSCs), alter the gene expression levels of myogenic regulatory factors, inhibit histone deacetylase (HDAC) activity, and increase the expression of Smad family member 7 (SMAD7) in PSCs. Furthermore, SFN was found to increase the acetylation level of histone H4 in the SMAD7 promoter and decrease the expression of certain microRNAs that target SMAD7 in PSCs.
Article
Biotechnology & Applied Microbiology
Jiale Yun, Xiaoyu Huang, Chang Liu, Mingyue Shi, Wenxia Li, Jin Niu, Chunbo Cai, Yang Yang, Pengfei Gao, Xiaohong Guo, Bugao Li, Chang Lu, Guoqing Cao
Summary: In this study, circRNA expression profiles in the skeletal muscle of Jinfen White pigs at different stages of development were constructed using RNA-seq. Among the identified circRNAs, 584 circRNAs showed differential expression. Enrichment analysis revealed that the host genes of these differentially expressed circRNAs were mainly involved in muscle contraction, muscle organ development, muscle system processes, as well as AMPK and cAMP-related signal pathways. A circRNA-miRNA-mRNA co-expression network was also constructed to identify key circRNAs involved in the regulation of porcine skeletal muscle development through the competitive endogenous RNA (ceRNA) mechanism. Notably, the circ_0018595/miR-1343/PGM1 axis may play a regulatory role in the development of porcine skeletal muscle.
Article
Agriculture, Dairy & Animal Science
Jingwei Yue, Xinhua Hou, Xin Liu, Ligang Wang, Hongmei Gao, Fuping Zhao, Lijun Shi, Liangyu Shi, Hua Yan, Tianyu Deng, Jianfei Gong, Lixian Wang, Longchao Zhang
Summary: Chromatin accessibility plays a crucial role in the regulation of embryonic muscle development in pigs by influencing the binding of transcription factors and controlling the temporal and spatial expression patterns of key genes involved in muscle development. The study highlights the importance of understanding the regulatory dynamics of genes in pig embryonic skeletal muscle development.
JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY
(2021)
Article
Genetics & Heredity
Yunhong Jing, Mailin Gan, Zhongwei Xie, Jianfeng Ma, Lei Chen, Shunhua Zhang, Ye Zhao, Lili Niu, Yan Wang, Li Zhu, Linyuan Shen
Summary: MicroRNAs (miRNAs) are small RNAs involved in various biological processes and diseases. In this study, the weight of intrauterine growth-restricted pig longissimus dorsi was found to be significantly lower than that of normal pigs. RNA sequencing identified 333 miRNAs, of which 26 were differentially expressed. Functional analysis revealed that these differentially expressed miRNAs regulate target genes involved in skeletal muscle proliferation and differentiation. Additionally, miRNA-451 was upregulated in IUGR pig skeletal muscle and promoted myoblastic differentiation in C2C12 cells.
Article
Biochemistry & Molecular Biology
Lingli Feng, Jinglei Si, Jingwei Yue, Mingwei Zhao, Wenjing Qi, Siran Zhu, Jiayuan Mo, Lixian Wang, Ganqiu Lan, Jing Liang
Summary: This study utilized RNA-seq and ATAC-seq to analyze muscle samples from Landrace pigs at different time points after birth. It identified ACBD7, TMEM220, and ATP1A2 as key genes related to porcine skeletal muscle development and also identified potential regulatory elements. Additionally, the results showed the importance of functional elements related to lipid metabolism and cell cycle in porcine skeletal muscle development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Dandan Wang, Yabin Pu, Yefang Li, Dengke Pan, Shengnan Wang, Wenjie Tian, Yuehui Ma, Lin Jiang
Summary: The mutation of IGF2 gene leads to increased muscle mass in pigs. The interaction between IGF2 and ZBED6 plays a role in skeletal muscle development. Through RNA-seq data analysis, lncRNAs with tissue-specific expression were identified in ZBED6 KO pigs, indicating their potential roles in tissue function and ZBED6-mediated skeletal muscle development.
Article
Genetics & Heredity
Weite Li, Shulei Wen, Jiahan Wu, Bin Zeng, Ting Chen, Junyi Luo, Gang Shu, Song-bo Wang, Yongliang Zhang, Qianyun Xi
Summary: The study revealed that skeletal muscle-derived exosomes can inhibit adipogenesis of porcine preadipocytes, with certain microRNAs enriched in lipid-related metabolism processes. These findings provide a fundamental resource for understanding the detailed functions of exosomes in the skeletal muscle-adipose crosstalk and the potential relationship between skeletal muscle atrophy and obesity.
FRONTIERS IN GENETICS
(2021)
Article
Biochemistry & Molecular Biology
Chunhuai Yang, Chunwei Cao, Jiali Liu, Ying Zhao, Jianfei Pan, Cong Tao, Yanfang Wang
Summary: Cold stress leads to piglet death and economic losses. This study revealed the different transcriptional responses of skeletal muscle to cold stimulation in Tibetan pigs and Bama pigs, providing novel insights for future investigation of the cold adaptation mechanism in pigs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Genetics & Heredity
Rui Yang, Yinuo Liu, Yunyun Cheng, Chunli Wang, Jie Song, Guanhong Lu, Tianqi Feng, Siyao Wang, Xiaotong Sun, Jilun Meng, Linlin Hao
Summary: Different SNPs in the MEG3 gene are associated with meat quality traits in pigs, affecting the proliferation and differentiation of porcine skeletal muscle satellite cells (SCs) in different ways.
FRONTIERS IN GENETICS
(2021)
Review
Endocrinology & Metabolism
Wataru Aoi, Yuko Tanimura
Summary: This passage highlights the importance of muscle-derived exosomes in regulating target protein expression and activity associated with metabolism and immune function. It also discusses the altered levels of circulating exosomes and their contents in muscle disorders and metabolic-related states, suggesting their potential as biomarkers.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Genetics & Heredity
Renqiang Yuan, Jiaman Zhang, Yujie Wang, Xingxing Zhu, Silu Hu, Jianhua Zeng, Feng Liang, Qianzi Tang, Yaosheng Chen, Luxi Chen, Wei Zhu, Mingzhou Li, Delin Mo
Summary: The study found that during the transition from E35 to E80, 11.43% of the genome switched compartment status, 14.53% of topologically associating domains experienced changes in intradomain interactions, and 2,730 genes showed differential promoter-enhancer interactions and (or) enhancer activity. The alterations in genome architecture were associated with the expression of genes involved in neuromuscular junction, embryonic morphogenesis, skeletal muscle development, or metabolism. Key genes like NEFL, MuSK, SLN, Mef2D, and GCK were identified as playing significant roles in these processes. Notably, Sox6 and MATN2 were highlighted for their important roles in the transition from primary to secondary myofibres formation. The study provided insight into genomic reorganization and constructed high-resolution interaction maps for studying gene expression control during this developmental period.
Article
Biochemistry & Molecular Biology
Mu Zeng, Shanying Yan, Peng Yang, Qiaowei Li, Jiju Li, Xinhao Fan, Xiaoqin Liu, Yilong Yao, Wei Wang, Ruipu Chen, Guohao Han, Yalan Yang, Zhonglin Tang
Summary: By conducting a comprehensive analysis of circRNA expression profiles in Chinese local breed Luchuan pigs and Western commercial breed Duroc pigs, we identified a total of 56,254 circRNAs, of which 42.9% were not previously annotated. Furthermore, we found that 33.7% of these circRNAs were differentially expressed between the two breeds, and they might contribute to the phenotypic differentiation. Additionally, we discovered a tissue-specific circRNA, circPSME4, which regulates skeletal muscle development.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Di Sun, Jiaqi An, Zixu Cui, Jiao Li, Ziwei You, Chang Lu, Yang Yang, Pengfei Gao, Xiaohong Guo, Bugao Li, Chunbo Cai, Guoqing Cao
Summary: This study identifies a novel circRNA, circCSDE1, that is highly expressed in skeletal muscle. Functional studies show that circCSDE1 promotes proliferation and inhibits myoblast differentiation, while miR-21-3p has opposite effects. Dual-luciferase reporter assay confirms that circCSDE1 targets miR-21-3p directly.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Agronomy
Dario Fornara, Elizabeth M. E. Ball, Christina Mulvenna, Henry Reyer, Michael Oster, Klaus Wimmers, Hanne Damgaard Poulsen, Arno Rosemarin
Summary: The over-supplementation of animal feeds with phosphorus leads to high rates of P excretion and negative environmental impacts. Adding phytase to pig and poultry diets can reduce P excretion, but the effects on plant-soil systems are poorly understood. This study found that phytase supplementation significantly influenced P content of animal waste and plant P dynamics, with intermediate phytase levels contributing to higher P use efficiency and lower soil P availability. The effects of dietary P content on P losses from soils varied depending on the type of organic amendment.
Article
Agriculture, Dairy & Animal Science
Christina C. Mulvenna, Ursula M. McCormack, Elizabeth Magowan, John McKillen, Mike R. Bedford, Carrie L. Walk, Michael Oster, Henry Reyer, Klaus Wimmers, Dario A. Fornara, M. Elizabeth E. Ball
Summary: Phytase has been a common addition to poultry diets for over 20 years, showing benefits in growth performance, nutrient digestibility, and bone parameters. This study found that superdosing phytase in rapeseed meal and maize dried distiller grains with solubles diets can improve performance, increase digestibility, and reduce P excretion by 15% compared to birds without phytase.
Article
Biochemistry & Molecular Biology
Maruf Hasan, Michael Oster, Henry Reyer, Siriluck Ponsuksili, Eduard Murani, Petra Wolf, Dagmar-Christiane Fischer, Klaus Wimmers
Summary: This study investigated the tissue-wide expression patterns of key genes related to vitamin D metabolism and FGF23 signaling in pigs fed different phosphorus diets. The results showed that non-renal tissues and cells have specific requirements for active vitamin D formation. Low phosphorus diet suppressed vitamin D signaling locally, while high phosphorus diet stimulated local vitamin D synthesis and regulation of FGF23 signaling in bone.
Article
Oncology
Yixuan Ma, Benjamin Schulz, Nares Trakooljul, Moosheer Al Ammar, Anett Sekora, Sina Sender, Frieder Hadlich, Dietmar Zechner, Frank Ulrich Weiss, Markus M. Lerch, Robert Jaster, Christian Junghanss, Hugo Murua Escobar
Summary: Small molecule inhibitors and targeted therapy have significant potential for pancreatic ductal adenocarcinoma (PDAC) treatment. The study found that two KRAS inhibitors, BI-3406 and sotorasib, demonstrated different anti-tumor efficacy and displayed synergistic or additive effects when combined with downstream inhibitors, emphasizing the importance of KRAS as a therapeutic target for PDAC.
Article
Agriculture, Dairy & Animal Science
Siriluck Ponsuksili, Frieder Hadlich, Alvaro Perdomo-Sabogal, Henry Reyer, Michael Oster, Nares Trakooljul, Muhammad Arsalan Iqbal, Sonja Schmucker, Volker Stefanski, Christoph Roth, Amelia Camarinha Silva, Korinna Huber, Vera Sommerfeld, Markus Rodehutscord, Klaus Wimmers
Summary: Aggregation of various data sets provides a comprehensive understanding of lifelong adaptation processes in laying hens. Two strains of laying hens, LB and LSL, were analyzed at different ages, revealing strain-specific and stage-specific biosignatures, including molecular pathways. Although the strains performed similarly, they showed differences in immunological and metabolic functions, as well as gut-microbiota interactions, indicating different strategies under high performance conditions. The integrative analysis not only provides insights into functional biodiversity, but also offers guidance for further manual review of the data.
Article
Agriculture, Dairy & Animal Science
Adewunmi O. Omotoso, Henry Reyer, Michael Oster, Steffen Maak, Siriluck Ponsuksili, Klaus Wimmers
Summary: Phosphorus inclusion in broiler diets needs to be tailored to their physiological demands at different developmental stages. A timed nutritional conditioning strategy, such as P depletion, may enhance mineral homeostasis mechanisms and reduce dietary P supply. This study investigated the effects of dietary P depletion on broiler chickens at different developmental stages, revealing marked responses early on, but indications of compensatory mechanisms later. Endocrine control mediated by calcitriol, intestinal P uptake, and mineral mobilization from bones were identified as potential mechanisms for maintaining mineral homeostasis. Precise timing, duration, and extent of P depletion should be considered for optimized nutrient utilization in broiler husbandry.
Article
Chemistry, Analytical
Mohd. Rahil Hasan, Pradakshina Sharma, Shifa Shaikh, Saumitra Singh, Roberto Pilloton, Jagriti Narang
Summary: We have developed a paper-based experimental platform for analyzing dengue virus, which incorporates screen-printing technology and black carbon conductive ink. The platform utilizes both two-electrode and three-electrode systems, with an LOD of 0.1 μg/mL for detecting the virus. The three-electrode system exhibits a significantly higher current range and amplification compared to the two-electrode system, making it a viable option for sensitive detection of dengue virus and other diseases.
Article
Nutrition & Dietetics
Maruf Hasan, Michael Oster, Henry Reyer, Klaus Wimmers, Dagmar-Christiane Fischer
Summary: Vitamin D-3 (Vit D-3) and 25(OH)D-3 are used as dietary sources of active vitamin D in pig husbandry, and while their primary effects are on the intestine, kidney, and bone, they also have wide-ranging effects on peripheral tissues. The use of Vit D-3 and 25(OH)D-3 in pig nutrition has been shown to have different outcomes in terms of reproductive capacities, growth performance, immunity, and bone development.
BRITISH JOURNAL OF NUTRITION
(2023)
Correction
Nutrition & Dietetics
Maruf Hasan, Michael Oster, Henry Reyer, Klaus Wimmers, Dagmar-Christiane Fischer
BRITISH JOURNAL OF NUTRITION
(2023)
Article
Agriculture, Dairy & Animal Science
Hanne Honerlagen, Henry Reyer, Ibrahim Abou-Soliman, Dierck Segelke, Siriluck Ponsuksili, Nares Trakooljul, Norbert Reinsch, Bjoern Kuhla, Klaus Wimmers
Summary: Improving the nitrogen-utilization efficiency (NUE) of dairy cows through breeding selection has benefits in terms of nutrition, environment, and economics. The individual milk urea concentration (MU) of cows has been proposed as an indicator trait for NUE. This study aimed to identify different microbial genera in the rumen of Holstein cows with divergent genomic breeding values for MU (GBVMU). The identified microbial genera were further investigated for their correlations with MU and other NUE-associated traits, providing insight into genetically determined N-utilization in Holstein cows.
JOURNAL OF DAIRY SCIENCE
(2023)
Article
Cell Biology
Julia Brenmoehl, Elli Brosig, Nares Trakooljul, Christina Walz, Daniela Ohde, Antonia Noce, Michael Walz, Martina Langhammer, Stefan Petkov, Monika Roentgen, Steffen Maak, Christina E. E. Galuska, Beate Fuchs, Bjoern Kuhla, Siriluck Ponsuksili, Klaus Wimmers, Andreas Hoeflich
Summary: This study investigated the contribution of non-mitochondrial and mitochondrial metabolic pathways in contracting muscles during repeated training. The results showed that marathon mice displayed increased glucose and fatty acid oxidation, reduced lactate dehydrogenase activity, and inhibited lactate formation compared to trained control mice. This suggests that marathon mice have enhanced metabolic flexibility towards lipid catabolism regulated by lactate dehydrogenase.
Review
Agriculture, Dairy & Animal Science
Benjamin Diehl, Michael Oster, Andreas Vernunft, Klaus Wimmers, Hartwig Bostedt
Summary: The losses of piglets in commercial pig farming remain a concern and sustainable breeding and management strategies are needed. This review systematically presents the three phases of neonatal adaptation in piglets and highlights the key developments and influencing factors in each phase.
ARCHIVES ANIMAL BREEDING
(2022)
Review
Biochemistry & Molecular Biology
Fabio Sarais, Alvaro Perdomo-Sabogal, Klaus Wimmers, Siriluck Ponsuksili
Summary: This review focuses on the latest discoveries of tsRNAs, with special attention to molecules initially thought to be associated with tiRNAs. The paper outlines their biological functions, provides a collection of useful databases, and discusses future research perspectives and applications in livestock basic and applied research.
