Article
Chemistry, Multidisciplinary
Chun Liu, Jingsheng Niu, Tingting Cui, Jinsong Ren, Xiaogang Qu
Summary: In this study, a near-infrared light-controllable carbonaceous nanocalabash motor catalyst was developed for active targeted synthesis of drugs inside biofilms. The catalyst exhibited good biocompatibility and rapid autonomous motion, enabling the generation of active molecules in deep biofilm layers to remove biofilms and eradicate bacteria.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Spectroscopy
Xiangrong Li, Wei Guo, Ruonan Xu, Zhizhi Song, Tianjun Ni
Summary: This study investigated the interaction between gold nanoparticles and various proteins using spectroscopic techniques. The results showed that gold nanoparticles can form a stable protein corona by interacting with proteins, and the nature of the interaction depends on the characteristics of the proteins.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Chemistry, Multidisciplinary
Xiaochen Tan, Kevin Welsher
Summary: A new method is introduced to track individual nanoparticles in real-time and measure their protein coronas, revealing that the number of proteins in the dynamic in situ protein corona is twice that of the ex situ measured hard protein corona.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Multidisciplinary Sciences
Yue Weng, Chun-Jen Su, Haoyang Jiang, Chien-Wei Chiang
Summary: Site-selective chemical bioconjugation of peptides and proteins, particularly phenylalanine bioconjugation, has great potential in improving the therapeutic effectiveness of modified protein drugs. However, covalent bioconjugation of native phenylalanine remains a challenging problem in protein modification. In this study, a photoredox method for site-selective bioconjugation of phenylalanine is described, which has been successfully validated on peptides and protein insulin under straightforward and mild conditions. The pyrazole labeling approach used in this study allows the complete dissociation of insulin hexamer into the monomeric form, making it a potential candidate for rapid-acting insulin in diabetes treatment.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Applied
Shijun Lin, Shuwen Liu, Guofei Dai, Xiaojin Zhang, Fan Xia, Yu Dai
Summary: This study presents a click-induced fluorescence-quenching sensor based on gold nanoparticles for rapid detection of copper ions and ascorbic acid. The sensor has a fast response time and high sensitivity, able to detect the concentrations of Cu2+ and AA within less than 3 minutes.
Review
Chemistry, Applied
Farid Ahmed, Hai Xiong
Summary: This review summarizes the latest developments in chemosensors based on click generated triazoles for a range of metal cations, anions, and neutral analytes, highlighting the need for continuous research accumulation in this rapidly growing field to meet demand.
Article
Biochemistry & Molecular Biology
Erwin Doe, Hannah L. L. Hayth, Ross Brumett, Emil F. F. Khisamutdinov
Summary: Nucleic acid-based therapeutics involve conjugating small molecule drugs to nucleic acid oligomers to overcome solubility challenges and the inefficient delivery into cells. Click chemistry has emerged as a popular approach due to its simplicity and high conjugation efficiency. However, the purification of oligonucleotide conjugates is a major drawback. This study introduces a simple and rapid purification method using MWCO centrifugation to separate excess unconjugated molecules and toxic catalysts for nucleic acid nanotechnology applications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Yawen You, Qingqing Deng, Yibo Wang, Yanjuan Sang, Guangming Li, Fang Pu, Jinsong Ren, Xiaogang Qu
Summary: This study reports on a DNA-based biocompatible, highly efficient, and precisely targeted copper nanocatalyst, which exhibits excellent catalytic activity and achieves efficient prodrug activation and antitumor effects in cancer cells.
NATURE COMMUNICATIONS
(2022)
Article
Chemistry, Multidisciplinary
Sudripet Sharma, Jacek B. Jasinski, Wilfried M. Braje, Sachin Handa
Summary: A simple and environmentally friendly method for synthesizing nanoparticles on the surface of the food additive HPMC and their application in organic solvent-free cycloaddition reactions were reported. The method showed high efficiency and the obtained products were of high purity.
Review
Chemistry, Multidisciplinary
Mindy Levine
Summary: The article reviews the use of fluorescence-based pesticide detection methods, with a focus on supramolecular chemistry-based methods. It provides illustrative examples of successful applications in real-world environments and highlights areas for future research and development.
FRONTIERS IN CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Tiberiu S. Mihaila, Carina Bate, Lynn M. Ostersehlt, Jasmin K. Pape, Jan Keller-Findeisen, Steffen J. Sahl, Stefan W. Hell
Summary: With the recent advancements in fluorescence nanoscopes, the ability to dissect nanoscopic biological structures is limited by the size of protein labels. Bioorthogonal chemical groups provide a powerful tool for specific detection of biomolecules. In this study, we introduced an engineered aminoacyl-tRNA synthetase/tRNA pair to allow for the site-specific introduction of amino acids with clickable side chains into proteins. This enhanced genetic code expansion machinery was then used to label beta-actin in cells for high-resolution imaging. Our findings highlight the need for further improvements in labeling methods to fully exploit the potential of molecular-scale optical three-dimensional resolution.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Chemistry, Analytical
Fengjing Cao, Fu Jiao, Shixiang Ma, Daming Dong
Summary: A simple and highly sensitive assay for copper(II) ions detection based on click chemistry and laser-induced breakdown spectroscopy (LIBS) is outlined. The method shows a good detection limit and has the potential to broaden the applications of LIBS in metal detection in drinking water and environment.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Multidisciplinary Sciences
Yuwen Wang, Patrick Czabala, Monika Raj
Summary: In this study, the authors report a one-pot multicomponent Furan-Thiol-Amine reaction inspired by enzymes, which can generate stable pyrrole heterocycles independent of diverse functionalities. The reaction has wide applicability, including selective and irreversible labeling of peptides, synthesis of macrocyclic and stapled peptides, selective modification of different proteins with varying payloads, and labeling of lysine and cysteine in a complex human proteome.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Qiaoqiao Ruan, Cheng Zhao
Summary: A protocol is provided for better control of protein labeling using Copper-free Click Chemistry reagents. This approach offers a simpler and more economical way to perform microscale labeling and has been shown to provide more stable conjugation than standard NHS-fluorophore labeling.
SCIENTIFIC REPORTS
(2023)
Article
Chemistry, Multidisciplinary
Leona L. Rodrigues, Aaron S. Micallef, Michael C. Pfrunder, Vinh X. Truong, John C. McMurtrie, Tim R. Dargaville, Anja S. Goldmann, Florian Feist, Christopher Barner-Kowollik
Summary: A highly efficient ligation system based on visible light-induced rearrangement was introduced, generating a thiophenol for rapid thiol-Michael additions without the need for additives. The self-catalyzed ligation through pyridine mediated deprotonation of the photochemically generated thiophenol was described, leading to additive-free thiol-Michael reactions. The study explored various reaction conditions, solvents, and substrates, showcasing excellent end group fidelity in polymer modification.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Cell Biology
Luisa Klotz, Melanie Eschborn, Maren Lindner, Marie Liebmann, Martin Herold, Claudia Janoschka, Belen Torres Garrido, Andreas Schulte-Mecklenbeck, Catharina C. Gross, Johanna Breuer, Petra Hundehege, Vilmos Posevitz, Beatrice Pignolet, Giulia Nebel, Shirin Glander, Nicole Freise, Judith Austermann, Timo Wirth, Graham R. Campbell, Tilman Schneider-Hohendorf, Maria Eveslage, David Brassat, Nicholas Schwab, Karin Loser, Johannes Roth, Karin B. Busch, Monika Stoll, Don J. Mahad, Sven G. Meuth, Timothy Turner, Amit Bar-Or, Heinz Wiendl
SCIENCE TRANSLATIONAL MEDICINE
(2019)
Article
Engineering, Electrical & Electronic
Maximilian Bettenhausen, Soenke Gruessing, Elena Hardt, Julia Flesch, Friedhard Roemer, Carlos Alvarado Chavarin, Wolfgang M. Klesse, Changjiang You, Jacob Piehler, Giovanni Capellini, Bernd Witzigmann
JOURNAL OF INFRARED MILLIMETER AND TERAHERTZ WAVES
(2019)
Article
Biochemistry & Molecular Biology
Kirill Salewskij, Bettina Rieger, Frances Hager, Tasnim Arroum, Patrick Duwe, Jimmy Villalta, Sara Colgiati, Christian P. Richter, Olympia E. Psathaki, Jose A. Enriquez, Timo Dellmann, Karin B. Busch
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2020)
Article
Biochemistry & Molecular Biology
Karin B. Busch
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2020)
Article
Biochemical Research Methods
Julia Flesch, Marie Kappen, Christoph Drees, Changjiang You, Jacob Piehler
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2020)
Article
Biochemistry & Molecular Biology
Hai Pan, Miao Jin, Ashwin Ghadiyaram, Parminder Kaur, Henry E. Miller, Hai Minh Ta, Ming Liu, Yanlin Fan, Chelsea Mahn, Aparna Gorthi, Changjiang You, Jacob Piehler, Robert Riehn, Alexander J. R. Bishop, Yizhi Jane Tao, Hong Wang
NUCLEIC ACIDS RESEARCH
(2020)
Article
Chemistry, Multidisciplinary
Maniraj Bhagawati, Simon Hoffmann, Katharina S. Hoeffgen, Jacob Piehler, Karin B. Busch, Henning D. Mootz
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2020)
Article
Cell & Tissue Engineering
Yi Miao, Andrew Ha, Wim de Lau, Kanako Yuki, Antonio J. M. Santos, Changjiang You, Maarten H. Geurts, Jens Puschhof, Cayetano Pleguezuelos-Manzano, Weng Chuan Peng, Ramazan Senlice, Carol Piani, Jan W. Buikema, Oghenekevwe M. Gbenedio, Mario Vallon, Jenny Yuan, Sanne de Haan, Wieger Hemrika, Kathrin Rosch, Luke T. Dang, David Baker, Melanie Ott, Philippe Depeille, Sean M. Wu, Jarno Drost, Roeland Nusse, Jeroen P. Roose, Jacob Piehler, Sylvia F. Boj, Claudia Y. Janda, Hans Clevers, Calvin J. Kuo, K. Christopher Garcia
Article
Multidisciplinary Sciences
Stefan Peischard, Huyen Tran Ho, Ilaria Piccini, Nathalie Strutz-Seebohm, Albrecht Roepke, Ivan Liashkovich, Hiteshika Gosain, Bettina Rieger, Karin Klingel, Britta Eggers, Katrin Marcus, Wolfgang A. Linke, Frank Ulrich Mueller, Stephan Ludwig, Boris Greber, Karin Busch, Guiscard Seebohm
SCIENTIFIC REPORTS
(2020)
Article
Nanoscience & Nanotechnology
Julia Flesch, Maximilian Bettenhausen, Marcin Kazmierczak, Wolfgang M. Klesse, Oliver Skibitzki, Olympia E. Psathaki, Rainer Kurre, Giovanni Capellini, Subhajit Guha, Thomas Schroeder, Bernd Witzigmann, Changjiang You, Jacob Piehler
Summary: Label-free optical detection of biomolecules is currently limited by a lack of specificity rather than sensitivity. The engineered three-dimensional IR-resonant silicon micropillar arrays (Si-MPAs) for protein sensing utilize the characteristic refractive index dispersion in the mid-infrared (IR) regime. The spatially controlled surface functionalization enables efficient targeting of soluble and membrane proteins into sensing hotspots directly from cells cultured on Si-MPAs.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Physics, Applied
Lara Jorde, Zehao Li, Adrian Poeppelwerth, Jacob Piehler, Changjiang You, Carola Meyer
Summary: By designing a surface architecture for reversible protein immobilization on CNTs, we have successfully fabricated reusable biosensors for multiple protein analysis. The specific capturing of His-tagged proteins was achieved by combining Py-PEG-tris-NTA and Ni(II) ions, with the protein binding capacity being restored by imidazole wash.
JOURNAL OF APPLIED PHYSICS
(2021)
Article
Biology
Nadia Fuellbrunn, Zehao Li, Lara Jorde, Christian P. Richter, Rainer Kurre, Lars Langemeyer, Changyuan Yu, Carola Meyer, Jorg Enderlein, Christian Ungermann, Jacob Piehler, Changjiang You
Summary: This study introduces a method called GIET to probe the axial orientation of arrested macromolecules at lipid monolayers, and analyzes the conformational organization of proteins and complexes involved in tethering and fusion at yeast vacuoles. The results reveal that the membrane-anchored Ypt7 protein undergoes conformational reorganization upon interactions with effector proteins, and the tethering complex alternates between 'closed' and 'open' conformations dynamically when recruited to membranes by Ypt7.
Article
Biochemistry & Molecular Biology
Parminder Kaur, Ryan Barnes, Hai Pan, Ariana C. Detwiler, Ming Liu, Chelsea Mahn, Jonathan Hall, Zach Messenger, Changjiang You, Jacob Piehler, Robert C. Smart, Robert Riehn, Patricia L. Opresko, Hong Wang
Summary: The telomere specific shelterin complex including TIN2 plays a crucial role in regulating telomeric DNA structures and interactions between TRF1, TRF2, and TPP1. Knockdown of TIN2 results in the removal of TRF1 and TRF2 from telomeres, affecting downstream effects in cell-based assays.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Biochemical Research Methods
Junel Sotolongo Bellon, Oliver Birkholz, Christian P. Richter, Florian Eull, Hella Kenneweg, Stephan Wilmes, Ulrich Rothbauer, Changjiang You, Mark R. Walter, Rainer Kurre, Jacob Piehler
Summary: Localization and tracking of individual receptors by single-molecule imaging allows for the study of assembly and dynamics of signaling complexes in the plasma membrane. This article presents a comprehensive workflow for imaging and analyzing receptor diffusion and interaction in live cells using up to four colors. The use of engineered GFP variants and fluorescent dyes enables the quantitative resolution of the stoichiometry and dynamics of the IFNg receptor signaling complex in living cells.
CELL REPORTS METHODS
(2022)
Article
Chemistry, Multidisciplinary
Michael Philippi, Changjiang You, Christian P. Richter, Mercedes Schmidt, Jannis Thien, Domenik Lisse, Joachim Wollschlaeger, Jacob Piehler, Martin Steinhart
