Article
Chemistry, Applied
Xixi Jia, Xiaowei Li, Liang Zhao, Saiwa Liu, Zhinan Wang, Jingjing Du, Lirui Ge, Kunxia Zhao, Lu Tian, Jian Xu, Zuhao Yan, Jiangyi Ding, Xi Xia
Summary: A sensitive and selective method using hydrophilic interaction chromatography combined with tandem mass spectrometry was developed to detect 18 aminoglycosides in bovine milk. Aminoglycosides were extracted and isolated from milk using a mixture of trichloroacetic acid, ammonium acetate, ethylenediaminetetraacetic acid, and sodium chloride, and an Oasis HLB solid-phase extraction cartridge. The method showed satisfactory separation of the analytes with detection and quantification limits ranging from 0.05-2 μg/kg and 0.1-5 μg/kg, respectively. The method was successfully applied to detect neomycin in one fresh bovine milk sample, and it provides a powerful tool for the surveillance of aminoglycosides in bovine milk.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2023)
Article
Biochemical Research Methods
Ming Huang, Xiaobin Xu, Haibo Qiu, Ning Li
Summary: In this study, a generic HILIC-MS/MS method was developed for in-depth characterization of nucleic acid therapeutic modalities. High-resolution mass spectrometry was used to determine the deconvoluted masses of oligonucleotide and siRNA standards and their impurities. Optimized higher-energy C-trap dissociation was used to improve the sequence coverage of DNA and RNA oligonucleotides, achieving higher on-column sensitivity for phosphorothioate oligonucleotide analysis.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Environmental Sciences
Yang Yue, Li Li, Baocheng Qu, Ying Liu, Xuankai Wang, Houyu Wang, Siyu Chen
Summary: Artificial sweeteners (ASs) were found in influent and effluent water samples of wastewater treatment plants (WWTPs) in Dalian, China. Sucralose (SUC) was the most abundant type of ASs, while removal efficiency for SUC was poor. The concentrations of ACE and SUC were higher in spring and summer, and ASs showed lower levels in winter, possibly due to higher ice-cream consumption.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Chemistry, Analytical
M. Mar Aparicio-Muriana, Rocio Carmona-Molero, Francisco J. Lara, Ana M. Garcia-Campana, Monsalud del Olmo-Iruela
Summary: The development of a HILIC-MS/MS multiclass method for simultaneous analysis of eight cyanotoxins in reservoir water samples is described in this work, providing a reliable and sensitive analytical approach for cyanotoxin analysis. The study shows that a tandem solid-phase extraction system can effectively extract and preconcentrate different classes of cyanotoxins.
Article
Chemistry, Analytical
Dun-Jian Wang, Da-Wei Wang, Qiu-Chen Fang, Ye Shen, Nv-Jin Zeng, Yan-Ling Yang, Hong-Wen Zhang, Yong-Qing Wang, Lu-Ning Sun
Summary: A simple, fast, and sensitive tandem mass spectrometry method was established to detect the concentration of regadenoson in human plasma, which was used to study the pharmacokinetic characteristics of regadenoson in healthy Chinese subjects. The method showed linear calibration curve, low precision, high accuracy, and no matrix effect for regadenoson or regadenoson-d3.
JOURNAL OF SEPARATION SCIENCE
(2022)
Article
Chemistry, Applied
Jie Wang, Jie Liao, Honghai Wang, Xiaofang Zhu, Linqiu Li, Weibo Lu, Gongshuai Song, Qing Shen
Summary: A method for analyzing plasmalogens in shellfish was developed, with mussel identified as having the highest content and being a good choice for large-scale preparation.
Article
Environmental Sciences
Dandan Li, Qiuda Zheng, Zhe Wang, Yuan Ren, Kevin V. Thomas, Phong K. Thai
Summary: Understanding the consumption patterns of artificial sweeteners in China is important for public health policies. Wastewater analysis revealed that university students consume more artificial sweeteners than the general population, and there is a correlation between temperature increase and higher consumption of artificial sweeteners.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Biochemical Research Methods
Camila Cristina Rodrigues Ferreira da Cunha, Mylena Gomes Freitas, Daniel Aparecido da Silva Rodrigues, Andre Luis Correa de Barros, Marcelo Carlos Ribeiro, Ananda Lima Sanson, Robson Jose de Cassia Franco Afonso
Summary: An analytical method based on LTPE combined with mass spectrometry was developed for determination of multiclass antibiotics in wastewater. The study showed that suspended wastewater particulate should not be neglected, and total concentrations of analytes in whole sample are important for accurate assessment.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Environmental Sciences
V Kerberova, H. Zlamalova Gargosova, J. CaslavskY
Summary: This study found that the acute toxicity of artificial sweeteners poses low risk to the aquatic ecosystem, sucralose has high concentration in wastewater but low ecotoxicity, saccharin has high ecotoxicity but is almost completely removed during wastewater treatment process. Further studies are needed to evaluate chronic toxicity of artificial sweeteners and possible synergistic or antagonistic effects in mixtures.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL SCIENCE AND TECHNOLOGY
(2022)
Article
Environmental Sciences
Dandan Li, Jake W. O'Brien, Benjamin J. Tscharke, Phil M. Choi, Fahad Ahmed, Jack Thompson, Jochen F. Mueller, Hongwen Sun, Kevin V. Thomas
Summary: A 7-year study on artificial sweetener consumption in Australia showed that the consumption of acesulfame and saccharin increased annually, while cyclamate consumption decreased. This study highlights the feasibility of quantitatively measuring artificial sweetener consumption over time through wastewater analysis.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Biochemical Research Methods
Naoki Ochi
Summary: A simple and effective clean-up procedure was developed for the simultaneous determination of ten paralytic shellfish toxins and tetrodotoxin in scallop and short-necked clam samples. The method showed minimal matrix effects and allowed quantification of the toxins through simple external calibration.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Biochemical Research Methods
Elif Ozturk Er, Belma Ozbek, Sezgin Bakirdere
Summary: A novel analytical method was described for the determination of underivatized amino acids in human plasma and urine samples, achieving accurate results through liquid chromatography-tandem mass spectrometry, demonstrating high reliability and reproducibility.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Biochemical Research Methods
Marcio David Bocelli, Deyber Arley Vargas Medina, Julie Paulin Garcia Rodriguez, Fernando Mauro Lancas, Alvaro Jose Santos-Neto
Summary: Dynamic single-drop microextraction (SDME) was automated using an Arduino-based Cartesian robot and combined with liquid chromatography-tandem mass spectrometry for the determination of parabens in wastewater samples. The robot-assisted technique facilitated the control of dynamic SDME, making the process more feasible, robust, and reliable. This setup proved to be a competitive strategy for the automated extraction of organic pollutants from water samples.
Article
Food Science & Technology
Zhixing Gu, Xi Nie, Ping Guo, Yuehua Lu, Bo Chen
Summary: An analytical method using HPLC with diode array and evaporative light scattering detectors was established for the simultaneous determination of hydrophobic and hydrophilic compounds in the dried rhizome of Atractylodes macrocephala Koidz. The method provides a new choice for the quality control of traditional Chinese medicines and health foods.
Article
Chemistry, Analytical
Xianjiang Li, Sheng Wang, Zhen Guo, Xiuqin Li, Qinghe Zhang, Hongmei Li
Summary: A novel method for the determination of fosetyl-aluminum residue in wheat flour was developed, with satisfactory sensitivity and compliance with European legislation requirements. The method was successfully applied to analyzing 75 wheat flour samples produced in four provinces in China, demonstrating its potential for routine quality control.
Article
Chemistry, Analytical
Maria Marinaki, Ioannis Sampsonidis, Artemis Lioupi, Panagiotis Arapitsas, Nikolaos Thomaidis, Kyriaki Zinoviadou, Georgios Theodoridis
Summary: This study developed an optimized HS-SPME-GC-MS protocol to analyze Greek white and red wines of six monovarietal PDO and PGI varieties. The results showed differences between varieties and provided conclusions about aroma characteristics.
Article
Infectious Diseases
Kelsey Ng, Nikiforos A. Alygizakis, Nikolaos S. Thomaidis, Jaroslav Slobodnik
Summary: The presence of antibiotics in the environment could lead to the development of antibiotic-resistant bacteria and pose a public health crisis. A study was conducted in 48 wastewater treatment plants across 11 countries, revealing the detection of 47 antibiotics. This finding suggests the presence of antibiotics in European ecosystems, which could trigger undesirable responses, including antibiotic resistance.
Article
Cardiac & Cardiovascular Systems
Georgia Vogiatzi, George Lazaros, Evangelos Oikonomou, Marios Kostakis, Zacharenia Kypritidou, Evangelia Christoforatou, Panagiotis Theofilis, Ariadne Argyraki, Nikolaos Thomaidis, Dimitris Tousoulis
Summary: This study investigated the effects of drinking water hardness on carotid atherosclerotic burden and arterial stiffness. The results showed that individuals living in areas with lower water hardness had lower risk of carotid atherosclerotic burden and plaque formation. However, the association with arterial stiffness remains uncertain and further research is needed.
HELLENIC JOURNAL OF CARDIOLOGY
(2023)
Article
Environmental Sciences
Olga S. Arvaniti, Georgios Gkotsis, Maria-Christina Nika, Stelios Gyparakis, Thrassyvoulos Manios, Nikolaos S. Thomaidis, Michalis S. Fountoulakis, Athanasios S. Stasinakis
Summary: The presence of 220 emerging contaminants, including artificial sweeteners, personal care products, coffee and tobacco-related compounds, and industrial chemicals, was investigated in the hospital wastewater of a Greek General Hospital for the first time. The results showed that the most frequently detected substances were coffee and tobacco-related compounds, followed by artificial sweeteners, parabens, and industrial chemicals. High concentrations of artificial sweeteners in hospital effluents indicate that hospitals should be considered as important sources of these contaminants.
Article
Environmental Sciences
Pradeep Dewapriya, Nikolaos Rousis, Cassandra Rauert, Nikolaos S. Thomaidis, Kevin V. Thomas
Summary: Urban stormwater runoff is a major source of water pollution caused by a large mixture of chemicals. The pollutant profile of stormwater samples collected from an Australian creek was analyzed using high-resolution mass spectrometry and chemometric tools. The study identified compounds such as nonpolymeric surfactants, plastic additives, rubber and resin-related products, and natural products in the stormwater. The prioritization workflow used in this study proved to be an effective tool for assessing key stormwater-related compounds and determining their environmental effects.
Article
Environmental Sciences
Nikolaos Rousis, Richard Bade, Ivan Romero-Sanchez, Jochen F. Mueller, Nikolaos S. Thomaidis, Kevin Thomas, Emma Gracia-Lor
Summary: The market for illicit drugs and new psychoactive substances (NPS) has increased significantly and festivals are identified as high-risk environments for substance use. Traditional surveillance methods have limitations, and wastewater-based epidemiology (WBE) proves to be a cost-effective and timely alternative. Utilizing WBE, a study was conducted in a large city in Spain during the New Year period and a summer Festival, identifying high consumption rates of specific NPS and illicit drugs, as well as dynamic changes in their use patterns over a six-month period. The study highlights the importance of WBE in complementing public health information and providing insights into drug use trends at festivals.
ENVIRONMENT INTERNATIONAL
(2023)
Article
Environmental Sciences
Wiebke Duerig, Sofia Lindblad, Oksana Golovko, Georgios Gkotsis, Reza Aalizadeh, Maria-Christina Nika, Nikolaos Thomaidis, Nikiforos A. Alygizakis, Merle Plassmann, Peter Haglund, Qiuguo Fu, Juliane Hollender, Jade Chaker, Arthur David, Uwe Kunkel, Andre Macherius, Lidia Belova, Giulia Poma, Hugues Preud'Homme, Catherine Munschy, Yann Aminot, Carsten Jaeger, Jan Lisec, Martin Hansen, Katrin Vorkamp, Linyan Zhu, Francesca Cappelli, Claudio Roscioli, Sara Valsecchi, Renzo Bagnati, Belen Gonzalez, Ailette Prieto, Olatz Zuloaga, Ruben Gil-Solsona, Pablo Gago-Ferrero, Sara Rodriguez-Mozaz, Helene Budzinski, Marie-Helene Devier, Georg Dierkes, Lise Boulard, Griet Jacobs, Stefan Voorspoels, Heinz Ruedel, Lutz Ahrens
ENVIRONMENT INTERNATIONAL
(2023)
Article
Chemistry, Applied
Anthi Panara, Evagelos Gikas, Nikolaos S. Thomaidis
Summary: Chemical characterization of six different trims of saffron was conducted using LC-QToF-MS. A total of 62 compounds were identified, including amino acids, vitamins, flavonoids, phenolics, carotenoids, and cyclohexenones. Multivariate Chemometrics techniques successfully discriminated between red trims (Pushal, Sargol and Negin) and between Style and Bunch. Proline and isophorone were highlighted as authenticity markers. The effect of three harvesting years on the most contributing compounds for trimming discrimination was evaluated.
Article
Fisheries
M. Kotsiri, A. Panara, A. Koupa, E. Gourzioti, N. s. Thomaidis, G. Rigos
Summary: This study aimed to determine the persistence of formaldehyde in seawater and gilthead seabream tissues after a formalin bath. Water and tissue samples were collected and analyzed using chromatographic techniques. The results showed that there was no increase in formaldehyde concentration in the fish fillets after immersion, and only negligible amounts of formaldehyde remained in the seawater. Further research is needed to investigate the degradation cycle of formaldehyde under Mediterranean seawater conditions.
MEDITERRANEAN MARINE SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Anthi Panara, Evagelos Gikas, Ilias Tzavellas, Nikolaos S. Thomaidis
Summary: Antioxidants are important for human health and the development of products with antioxidant activity has become prominent. A local industry has created new antioxidant drinks using pomegranate, blackberries, red grapes, and aronia. LC-qTOFMS was used to characterize the drinks' constituents, with suspect and target screening conducted using a modified MS-DIAL workflow. Compounds with potential antioxidant capacity, such as flavonoids, amino acids, and fatty acids, were identified, highlighting the significance of the drinks and the efficacy of the workflow developed.
Article
Biochemistry & Molecular Biology
Anthi Panara, Evagelos Gikas, Anastasia Koupa, Nikolaos S. Thomaidis
Summary: This study produced a novel fertilizer via the hydrolysis of an onion-derived compost, and evaluated its efficacy by applying it to tomato plants. The analysis of the plant's leaf metabolites demonstrated the effectiveness of the fertilizer product. The study also demonstrated the efficiency of the analytical and statistical methods used.
Article
Medicine, General & Internal
Sotirios G. Papageorgiou, Ioanna Mavroeidi, Marios Kostakis, Aris Spathis, Danai Leventakou, Evangelia Kritikou, Nikolaos Oikonomopoulos, Chrysoula Kourkouti, Maria Krania, Anthi Bouchla, Thomas Thomopoulos, Zoi Tsakiraki, Konstantinos Markakis, Ioannis G. Panayiotides, Nikolaos Thomaidis, Vasiliki Pappa, Periklis G. Foukas, Melpomeni Peppa
Summary: Primary adrenal lymphoma (PAL) is a rare disease presenting as unilateral or bilateral rapidly growing adrenal masses. This study reports two cases of PAL presenting as adrenal incidentalomas (AIs) with autonomous cortisol production. Both patients showed symptoms of lumbar pain and were diagnosed with PAL through imaging and hormonal evaluation. They underwent biopsies that confirmed the presence of diffuse large B-cell lymphoma. Treatment was administered, but one patient died from infection shortly after. This study highlights the need for further analysis of PAL cases to understand its pathogenesis and course.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Antigoni Margariti, Vasiliki D. Papakonstantinou, George M. Stamatakis, Constantinos A. Demopoulos, Christina Machalia, Evangelia Emmanouilidou, Gregor Schnakenburg, Maria-Christina Nika, Nikolaos S. Thomaidis, Athanassios I. Philippopoulos
Summary: This study investigates the antiplatelet and anti-inflammatory activities of a series of complexes. It is found that certain complexes exhibit potent inhibitory effects on platelet aggregation induced by Platelet-Activating Factor (PAF) and also affect the metabolic enzymatic pathways of PAF, resulting in anti-inflammatory properties.
Article
Chemistry, Applied
Sofia K. Drakopoulou, Anastasia S. Kritikou, Carsten Baessmann, Nikolaos S. Thomaidis
Summary: This study explores the potential of LC-TIMS-HRMS as a reliable analytical platform for authenticity studies and applied it in the adulteration study of extra virgin olive oil (EVOO). Using an integrated untargeted 4D-metabolomics approach, the study successfully built robust prediction models that could discriminate between authentic EVOOs and adulterated ones, while also identifying specific markers for each group. The addition of TIMS in the LC-HRMS workflow significantly increased the metabolic coverage and enabled the detection of adulteration down to 1%.
Article
Biochemistry & Molecular Biology
Ioanna Barla, Panagiotis Efentakis, Sofia Lamprou, Maria Gavriatopoulou, Meletios-Athanasios Dimopoulos, Evangelos Terpos, Ioanna Andreadou, Nikolaos Thomaidis, Evangelos Gikas
Summary: The antineoplastic agent carfilzomib (Cfz) is used to treat multiple myeloma; however, it can cause cardiotoxicity and nephrotoxicity as common adverse effects. While the mechanisms of these side effects are well-established, there is limited research on the drug's metabolic alterations and their impacts.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Analytical
Gang Qu, Yuxin Zhao, Qiaoli Zhang, Jina Wu, Xiaosen Li, Yang Yang, Shilei Liu
Summary: In this study, magnetic mesoporous materials combined with real-time in situ mass spectrometry were used for the high-throughput detection of hydrolyzed products of organophosphorus nerve agents. The method showed good linearity, low limits of detection and quantification, and high extraction recoveries. The magnetic preparation method used was quick, cost-effective, rugged, and safe. The results demonstrated the potential of this method for rapid and efficient determination of the target analytes in environmental samples.
ANALYTICAL METHODS
(2024)
Article
Chemistry, Analytical
Anna Hildebrand, Mariam Merchant, Danny O'Hare
Summary: Substandard and falsified artemisinin derivatives in antimalarials have caused significant deaths and economic losses. This study evaluates the feasibility of voltammetric methods for identifying and quantifying artemether. The findings suggest that electrochemical analysis shows promise as a method for artemether identification and quantification.
ANALYTICAL METHODS
(2024)
Article
Chemistry, Analytical
Marx Osorio Araujo Pereira, Alvaro Ferreira Junior, Edson Silvio Batista Rodrigues, Helena Mulser, Giovanna Nascimento de Mello e Silva, Wallans Torres Pio dos Santos, Eric de Souza Gil
Summary: Brazilian spotted fever (BSF) is a serious and rapidly evolving disease. A new impedimetric immunosensor was developed for rapid diagnosis by measuring specific antibodies in plasma. The sensor demonstrated selectivity and accuracy, and has potential for important applications in diagnostic testing.
ANALYTICAL METHODS
(2024)
Article
Chemistry, Analytical
Kathrin Schilling, Ronald A. Glabonjat, Olgica Balac, Marta Galvez-Fernandez, Arce Domingo-Relloso, Vesna Slavkovich, Jeff Goldsmith, Miranda R. Jones, Tiffany R. Sanchez, Ana Navas-Acien
Summary: Analysis of trace elements in urine is an important tool for assessing exposures, diagnosing nutritional status, and guiding public health and healthcare intervention. This study provides a sensitive method for analyzing 18 elements in urine samples, using only 100 μL. The results show good accuracy and sensitivity of the method.
ANALYTICAL METHODS
(2024)
Article
Chemistry, Analytical
Mengya Li, Shijie Liu, Shiliang Guo, Dong Liang, Miaoyun Li, Yaodi Zhu, Lijun Zhao, Jong-Hoon Lee, Gaiming Zhao, Yangyang Ma, Yanxia Liu
Summary: In this study, a magnetic flow device was developed to purify spores in a culture medium system. The device used magnetic nanoparticles to absorb vegetative cells, separating them from the spores. The achieved purity of the collected spores was over 95%. The study also demonstrated a rapid quantitative detection method using Raman spectroscopy.
ANALYTICAL METHODS
(2024)
Article
Chemistry, Analytical
Wanqiong Liu, Zixuan Wu, Jianwei Peng, Zebin Xu, Yong Liang
Summary: Metal-organic frameworks (MOFs) are effective carriers for molecular imprinting, but their poor dispersibility in aqueous solution is a significant drawback. In this study, we have applied amphiphilic block copolymers and molecularly imprinted technology on MOFs to improve the hydrophilicity of molecularly imprinted fluorescent materials.
ANALYTICAL METHODS
(2024)
