Article
Biotechnology & Applied Microbiology
Jianrong Chen, Weizhuo Tang, Chunyan Li, Ding Kuang, Xiaojiang Xu, Yuan Gong, Fang Liu, Song Gao
Summary: This study revealed the key genes related to the biosynthesis of flavonoids in gardenia by analyzing the transcriptome and metabolome. It was found that these genes showed a certain spatial and temporal pattern in gene expression during fruit development and ripening.
Article
Food Science & Technology
Haibo Zhou, Sen Zhang, Lianghua Chen, Yimei Liu, Luhong Shen, Jiuliang Zhang
Summary: Gardenia has been highly valued as a food herbal medicine due to its bioactive compounds, but the mechanism underlying its hypoglycemic effect is unknown. This study evaluated the effect of gardenia extracts on type 2 diabetes and compared the efficacy of different purified fragments. The results showed that geniposide had a better inhibitory effect on T2DM in animal experiments, while crocin I had a better effect in vitro. These findings suggest potentially different mechanisms between geniposide and crocin I in treating T2DM.
Article
Geriatrics & Gerontology
Pyeong Geun Choi, So-Hyun Park, Farida S. Nirmala, Hee Soo Kim, Min Jung Kim, Jeong-Hoon Hahm, Hyo-Deok Seo, Jiyun Ahn, Taeyoul Ha, Chang Hwa Jung
Summary: Since the 1900s, human life span has increased significantly, but healthy aging remains a challenge. This study investigated the effect of Gardenia jasminoides Ellis fruit ethanol extract (GFE) on the life span and healthspan of Caenorhabditis elegans (C. elegans). Results showed that GFE increased life span by 27.1% and improved healthspan-related markers. The main component of GFE, geniposide, also increased life span by 18.55% and improved healthspan markers.
JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES
(2023)
Article
Multidisciplinary Sciences
Yuan Pan, Xiao Zhao, Yu Wang, Jun Tan, Da-xia Chen
Summary: This study comprehensively compared the transcriptome and metabolites profiles of Gardenia jasminoides Ellis (G. jasminoides) fruits, revealing significant differences in metabolite accumulation and gene expression among different fruit colors and parts. The negative correlation between the biosynthesis of geniposide and crocin was found, suggesting the redirection of metabolic flux and regulation of key enzymes as main reasons for changes in iridoid and crocin biosynthesis in G. jasminoides fruit. Valuable information for functional genomic library and insights for metabolic engineering of secondary metabolites in G. Jasminoides were provided.
Article
Integrative & Complementary Medicine
Jing Liu, Chao Yang, Zhigui Wu, Jianguo Pei, Yao Chen, Xiao Huang, Sha Gao, Rui Kan, Wenna Zhang, Saisai Xie, Xiaomei Fu
Summary: The study investigated the protective effects of Gardenia jasminoides Ellis fruit extracts (GFE) on TNBS-induced colitis in rats. Results showed that GFE significantly alleviated colitis symptoms and may have anti-inflammatory and antioxidative properties, making it a promising protective agent for colitis warranting further research.
EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE
(2021)
Article
Plant Sciences
Luhong Zhang, Yang Ai, Yunzhu Chen, Changzhu Li, Peiwang Li, Jingzhen Chen, Lijuan Jiang, Yuhong Pan, An Sun, Yan Yang, Qiang Liu
Summary: This study investigates the accumulation and biosynthesis of geniposide and crocin during fruit development in Gardenia jasminoides. The cumulative amount of geniposide was highest (2.035%) during the unripe-fruit period, while the highest content of crocin (1.098%) was observed during the mature-fruit period. Transcriptome sequencing identified key enzymes involved in geniposide and crocin biosynthesis, and their expression levels were consistent with the accumulation of geniposide and crocin content. qRT-PCR results further confirmed the trends of relative expression. This study provides important insights into the accumulation and biosynthesis of geniposide and crocin in G. jasminoides fruits.
Review
Food Science & Technology
Chengyu Jin, Abel Wend-Soo Zongo, Hengjun Du, Yuanchao Lu, Ningxiang Yu, Xiaohua Nie, Ashton Ma, Qin Ye, Hang Xiao, Xianghe Meng
Summary: This review provides a critical and comprehensive summary of research on Gardenia fruit (GF), including its nutrient content, extraction technologies, and potential health benefits. The study highlights the need for more efficient extraction methods and further research to understand the specific molecular mechanisms underlying its bioactivities. The review also proposes novel industrial applications for GF and emphasizes its potential as an innovative food resource.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Article
Agriculture, Multidisciplinary
Xianghe Meng, Congcong Wu, Haizhen Liu, Qiwen Tang, Xiaohua Nie
Summary: Dietary fiber from gardenia husk can be fractionated into four parts, each showing differences in structure and in vitro hypoglycemic effect. Water-soluble fiber has advantages in glucose adsorption and α-amylase activity inhibition, while alkali-soluble fiber is more effective in retarding glucose diffusion across a membrane.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2021)
Article
Agricultural Engineering
Danli Wang, Yawen Yuan, Tingfeng Xie, Guangwei Tang, Gongshuai Song, Ling Li, Tinglan Yuan, Fuping Zheng, Jinyan Gong
Summary: In this study, ultrasonic pretreatment was used to accelerate the aqueous enzymatic extraction of gardenia fruit oil. The extraction conditions of two enzymes were optimized using the response surface method. Ultrasonic pretreatment reduced the protein content in the raw material and increased the fluidity of the powder. The oil extracted by ultrasound-assisted aqueous enzymatic extraction showed better quality and higher contents of tocopherol, squalene, and polyunsaturated fatty acids.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Article
Agricultural Engineering
Wenjie Xu, Qian Lou, Lijun Hao, Kaizhi Hu, Min Cao, Yanqin Liu, Rongrong Han, Chunnian He, Jingyuan Song
Summary: Through multidimensional transcriptional and metabolic analyses of Gardenia jasminoides at different developmental stages, researchers identified ten genes involved in geniposide biosynthesis and successfully cloned two genes capable of catalyzing the conversion of geniposidic acid into geniposide. These two species-specific genes were found to be unique to Gardenia jasminoides, indicating the existence of a Gardenia-specific geniposide biosynthetic pathway.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Energy & Fuels
Can Qin, Shunrui Wang, Zhipeng Wang, Kai Ji, Shunjie Wang, Xueying Zeng, Xingmao Jiang, Gang Liu
Summary: The study demonstrated an efficient and sustainable approach for high performance supercapacitors by using biomass waste carbon, resulting in hierarchical porous carbon with large specific surface area and excellent electrochemical performance.
JOURNAL OF ENERGY STORAGE
(2021)
Article
Horticulture
Kangqin Li, Liqin Gao, Xiaoli Yang, Meixia Wang, Xiaotao Feng, Shaoyong Deng
Summary: In this study, 19 samples of Gardenia jasminoides from China were resequenced, revealing the presence of diversity sites mainly located in the intergenic region. The samples were classified into four categories, and the study provided a theoretical foundation for molecular markers, genetic localization, and utilization of the plant.
Article
Multidisciplinary Sciences
Xinyu Xu, Bihua Chen, Juan Zhang, Siren Lan, Shasha Wu
Summary: This study evaluated the genomic characteristics of Gardenia jasminoides by re-sequencing wild and cultivated materials. The results showed that Gardenia jasminoides has rich genetic diversity and identified numerous SNPs and other gene variations. Additionally, the study further explored gene variations in the biosynthetic pathway of the main medicinal substances in Gardenia jasminoides.
Article
Biochemistry & Molecular Biology
Xinyu Xu, Bihua Chen, Juan Zhang, Siren Lan, Shasha Wu, Weiwei Xie
Summary: Based on transcriptome sequencing and metabolic group analysis, this study analyzed the morphological and structural changes of Gardenia fruit and studied the formation mechanism and content changes of Geniposide and Crocin in Gardenia fruit. It was found that the content of Geniposide decreased with fruit development, while the content of Crocin increased. The study provides a theoretical basis for the utilization and breeding of Gardenia fruit.
MOLECULAR BIOLOGY REPORTS
(2023)
Article
Pharmacology & Pharmacy
Nan Zhang, Ying Bian, Lei Yao
Summary: The essential oils extracted from two gardenia varieties have anti-inflammatory effects on murine alveolar macrophages, inhibiting nitric oxide release and reducing the production of TNF-alpha and PGE2. Components such as linalool and alpha-farnesene in the gardenia flower essential oils have potential as anti-inflammatory agents.
Article
Chemistry, Analytical
Yanxiao Jiang, Bo Zhang, Jingkang Li, Ying Sun, Xinghua Wang, Pinyi Ma, Daqian Song
Summary: A novel vortex-assisted solid-phase extraction method was developed for the determination of parabens in water samples. The method combined the preparation of sponge column materials with high performance liquid chromatography analysis, successfully detecting four parabens in different water samples with low limits of detection and high accuracy.
Article
Biochemical Research Methods
Dan Li, Fangfang Chen, Na Li, Xiwen Ye, Ying Sun, Pinyi Ma, Daqian Song, Xinghua Wang
Summary: A new type of fluorescent silicon nanoparticles was prepared via a one-pot hydrothermal method for constructing a ratiometric fluorescence assay, allowing rapid and sensitive determination of xanthine in human serum. The assay demonstrated superior sensitivity and reduced testing time compared to traditional methods, showing potential for future fabrication of novel fluorescent probes for detecting biological metabolites involved in H2O2-generation or consumption reactions.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Wei Wang, Yue Zhang, Wei Zhang, Yibing Liu, Pinyi Ma, Xinghua Wang, Ying Sun, Daqian Song
Summary: A sensing platform for the determination of alkaline phosphatase (ALP) in human serum based on SERS-fluorescent dual-mode assay was developed, allowing for evaluation of ALP inhibitors. The platform utilizes a nanoprobe that can provide both SERS and fluorescence signals without the need for additional reporters or fluorophores.
ANALYTICA CHIMICA ACTA
(2021)
Article
Biochemical Research Methods
Xueqi Zhao, Yue Zhang, Xinghua Wang, Pinyi Ma, Daqian Song, Ying Sun
Summary: In this study, a LRSPR biosensor was developed using PDA/Ti3C2-MXene/PDA-gold film as the sensing substrate and AuNPs as enhancers. The biosensor demonstrated excellent performance with a broad detection range and low detection limit.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Wei Zhang, Yu Zhang, Xin Liu, Yue Zhang, Yibing Liu, Wei Wang, Rui Su, Ying Sun, Yibing Huang, Daqian Song, Yanhua Wu, Xinghua Wang
Summary: A sensing platform with both ratiometric fluorescence and colorimetric responses towards copper(II) ions and D-penicillamine was developed based on carbon dots. The platform exhibited excellent selectivity and sensitivity, and showed potential for live cell imaging.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Yibing Liu, Yu Zhang, Xianlong Zhang, Wei Zhang, Xinghua Wang, Ying Sun, Pinyi Ma, Yibing Huang, Daqian Song
Summary: The study developed a probe based on Ag&Mn:ZnInS QDs for detecting TYR activity with good selectivity and low detection limit. The probe showed good biocompatibility in intracellular imaging experiments on A549 cells.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Siqi Zhang, Yu Zhang, Lihe Zhao, Lanlan Xu, Hao Han, Yibing Huang, Qiang Fei, Ying Sun, Pinyi Ma, Daqian Song
Summary: Mitochondrial viscosity is important for pathological activities, and JLQL can monitor viscosity effectively.
Article
Chemistry, Analytical
Lanlan Xu, Yu Zhang, Lihe Zhao, Hao Han, Siqi Zhang, Yibing Huang, Xinghua Wang, Daqian Song, Pinyi Ma, Ping Ren, Ying Sun
Summary: A novel dual-signal colorimetric fluorescent probe XH-2 was developed for detecting hydrogen peroxide, with a good linear range and low detection limit. The probe can also monitor hydrogen peroxide in a colorimetric manner, making it a superior tool for evaluating drug-induced liver injury.
Article
Biochemical Research Methods
Xiwen Ye, Yanxiao Jiang, Xiaowei Mu, Ying Sun, Pinyi Ma, Ping Ren, Daqian Song
Summary: A highly sensitive fluorescence-based assay for cholesterol detection was developed using water-dispersible green-emitting silicon nanoparticles (SiNPs) as a fluorescence indicator and enzyme-catalyzed oxidation product PPDox as a quencher. The established ultrasensitive fluorescent assay accurately measured cholesterol with a low limit of detection and a wide linear range, and demonstrated good accuracy in detecting real samples.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Li Jingkang, Jiang Yanxiao, Yang Jukun, Sun Ying, Ma Pinyi, Song Daqian
Summary: A novel environmental-friendly composite absorbent material was designed and prepared in this study. The material showed excellent adsorption performance and stable reusability. It was characterized using high performance liquid chromatography and other analytical techniques, and successfully applied for the solid phase extraction of pollutants from water samples.
CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
(2022)
Article
Chemistry, Analytical
Yibing Liu, Xue Jiang, Wei Wang, Wei Zhang, Xinghua Wang, Ying Sun, Yibing Huang, Pinyi Ma, Daqian Song
Summary: In this study, a multifunctional probe was developed for bimodal imaging of glutathione (GSH) by improving stability and targeted recognition capability. The probe showed good magnetic resonance imaging (MRI) contrast agent and near-infrared emission properties, making it a promising tool in biological detection.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Analytical
Haohua Yang, Xueqi Zhao, Ziwei Zhang, Pinyi Ma, Xinghua Wang, Daqian Song, Ying Sun
Summary: This paper presents a sensitive SPR biosensor based on ZnO@Au nanomaterial for the detection of hIgG. The biosensor utilizes a sandwich strategy using biotin-streptavidin for secondary signal amplification. With optimal experimental conditions, the biosensor achieves a favorable concentration range of 0.0375-40 μg/mL for hIgG determination, which is approximately 67 times lower than conventional gold film-based SPR sensors.
Article
Chemistry, Analytical
Lanlan Xu, Hongyu Chu, Dejiang Gao, Qiong Wu, Ying Sun, Zhenxin Wang, Pinyi Ma, Daqian Song
Summary: Fluorescence imaging-guided diagnostics is a promising method for detecting tumors in situ due to its simplicity and non-invasiveness. The chemosensor LAN-beta gal, with a specific recognition unit of d-galactose residue and a fluorophore of LAN-OH, showed remarkable fluorescence enhancement after beta-gal was digested. It successfully visualized endogenous beta-gal, distinguished ovarian cancer cells from normal cells, and was applied in vivo for tumor detection and surgical resection of ovarian cancer. The LAN-beta gal chemosensor shows potential as a new tool for the clinical diagnosis and treatment of ovarian cancer.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Wei Wang, Jingkang Li, Yibing Liu, Wei Zhang, Ying Sun, Pinyi Ma, Daqian Song
Summary: Alkaline phosphatase (ALP) is a widely present and important biomarker in the human body. Researchers synthesized a core-shell metal-organic framework sensor that can be used for sensitive and accurate ALP detection. By self-triggered control of phosphate ions (Pi), the sensor avoids the potential inaccuracy caused by using ascorbic acid (AA) as the reducing component. The developed strategy has shown linear response to ALP concentrations ranging from 0.1 to 150 mU/mL with a detection limit of 0.03 mU/mL.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Zhang Yue, Zhang Wei, Wang Xing-Hua, Ma Pin-Yi, Sun Ying, Song Da-Qian
Summary: A detection system based on g-C3N4 nanosheets, copper ions, and o-phenylenediamine (OPD) was developed for the detection of Coenzyme A (CoA). The system showed good sensitivity and selectivity with a proportional fluorescence response to CoA concentration. This method has the potential for detecting CoA in complex biological matrices.
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Gang Qu, Yuxin Zhao, Qiaoli Zhang, Jina Wu, Xiaosen Li, Yang Yang, Shilei Liu
Summary: In this study, magnetic mesoporous materials combined with real-time in situ mass spectrometry were used for the high-throughput detection of hydrolyzed products of organophosphorus nerve agents. The method showed good linearity, low limits of detection and quantification, and high extraction recoveries. The magnetic preparation method used was quick, cost-effective, rugged, and safe. The results demonstrated the potential of this method for rapid and efficient determination of the target analytes in environmental samples.
ANALYTICAL METHODS
(2024)
Article
Chemistry, Analytical
Anna Hildebrand, Mariam Merchant, Danny O'Hare
Summary: Substandard and falsified artemisinin derivatives in antimalarials have caused significant deaths and economic losses. This study evaluates the feasibility of voltammetric methods for identifying and quantifying artemether. The findings suggest that electrochemical analysis shows promise as a method for artemether identification and quantification.
ANALYTICAL METHODS
(2024)
Article
Chemistry, Analytical
Marx Osorio Araujo Pereira, Alvaro Ferreira Junior, Edson Silvio Batista Rodrigues, Helena Mulser, Giovanna Nascimento de Mello e Silva, Wallans Torres Pio dos Santos, Eric de Souza Gil
Summary: Brazilian spotted fever (BSF) is a serious and rapidly evolving disease. A new impedimetric immunosensor was developed for rapid diagnosis by measuring specific antibodies in plasma. The sensor demonstrated selectivity and accuracy, and has potential for important applications in diagnostic testing.
ANALYTICAL METHODS
(2024)
Article
Chemistry, Analytical
Kathrin Schilling, Ronald A. Glabonjat, Olgica Balac, Marta Galvez-Fernandez, Arce Domingo-Relloso, Vesna Slavkovich, Jeff Goldsmith, Miranda R. Jones, Tiffany R. Sanchez, Ana Navas-Acien
Summary: Analysis of trace elements in urine is an important tool for assessing exposures, diagnosing nutritional status, and guiding public health and healthcare intervention. This study provides a sensitive method for analyzing 18 elements in urine samples, using only 100 μL. The results show good accuracy and sensitivity of the method.
ANALYTICAL METHODS
(2024)
Article
Chemistry, Analytical
Mengya Li, Shijie Liu, Shiliang Guo, Dong Liang, Miaoyun Li, Yaodi Zhu, Lijun Zhao, Jong-Hoon Lee, Gaiming Zhao, Yangyang Ma, Yanxia Liu
Summary: In this study, a magnetic flow device was developed to purify spores in a culture medium system. The device used magnetic nanoparticles to absorb vegetative cells, separating them from the spores. The achieved purity of the collected spores was over 95%. The study also demonstrated a rapid quantitative detection method using Raman spectroscopy.
ANALYTICAL METHODS
(2024)
Article
Chemistry, Analytical
Wanqiong Liu, Zixuan Wu, Jianwei Peng, Zebin Xu, Yong Liang
Summary: Metal-organic frameworks (MOFs) are effective carriers for molecular imprinting, but their poor dispersibility in aqueous solution is a significant drawback. In this study, we have applied amphiphilic block copolymers and molecularly imprinted technology on MOFs to improve the hydrophilicity of molecularly imprinted fluorescent materials.
ANALYTICAL METHODS
(2024)