Article
Pharmacology & Pharmacy
E. Unlu, G. Turna Saltoglue, G. Unlu, H. Tozak Yildiz
Summary: This study determined the AFM1 levels in breast milk samples collected from mothers who gave birth in Kirsehir, Turkey. The results showed that the AFM1 levels in breast milk were lower in mothers who did not consume milk, mothers consuming fabrication milk had lower AFM1 levels compared to mothers consuming homemade milk (p<0.01), and mothers consuming homemade or self-made bread had lower AFM1 levels (p<0.05).
EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES
(2023)
Article
Chemistry, Applied
Jiadi Sun, Miao Li, Fuguo Xing, Haiming Wang, Yinzhi Zhang, Xiulan Sun
Summary: This study developed a dual immunochromatographic test strips method based on double antibodies labeled with time resolved fluorescent microspheres to realize simultaneous rapid detection of AFM1 and OTA in milk. The method showed low detection limits and good accuracy, repeatability, and specificity.
Article
Chemistry, Applied
Harpreet Singh, Shalini Singh, Sanjeev K. Bhardwaj, Gurjeet Kaur, Madhu Khatri, Akash Deep, Neha Bhardwaj
Summary: In this research, a novel fluorescent immunosensor based on nitrogen-doped carbon quantum dots has been developed for the analysis of aflatoxin M1 in dairy products. The sensor exhibited high sensitivity and low limit of detection, and was further developed as a lateral flow design for on-site detection.
Article
Food Science & Technology
Miao Li, Haiming Wang, Jiadi Sun, Jian Ji, Yongli Ye, Xin Lu, Yinzhi Zhang, Xiulan Sun
Summary: In this study, a time-resolved fluorescence microsphere immunochromatographic test strip (TRFM-ICTS) was developed for rapid and quantitative detection of AFM1 in milk. The TRFM-ICTS showed improved sensitivity and good recoveries, with results consistent with those obtained by UPLC-MS/MS.
Article
Food Science & Technology
Nikita Saha Turna, Felicia Wu
Summary: The study found that milk samples from several nations such as Pakistan, India, and some sub-Saharan African countries had AFM1 levels that significantly exceeded the regulatory limits set by the United States and the European Union. This indicates a potential risk to individuals in those nations with high milk consumption. High levels of AFM1 in milk also suggest high levels of AFB1 in animal feed, which could potentially harm both animals and humans upon consumption.
TRENDS IN FOOD SCIENCE & TECHNOLOGY
(2021)
Article
Food Science & Technology
Abu Hasan Sumon, Farjana Islam, Nayan Chandra Mohanto, Rahanuma Raihanu Kathak, Noyan Hossain Molla, Sohel Rana, Gisela H. Degen, Nurshad Ali
Summary: The study revealed that 78.6% of milk and dairy products in Bangladesh were contaminated with AFM(1) in concentrations ranging from 5.0 to 198.7 ng/L. However, yogurt and milk powder samples had lower levels of AFM(1) below the EU regulatory limit for milk. This highlights the need for further monitoring of aflatoxin contamination in milk and dairy products in Bangladesh.
Article
Chemistry, Analytical
Jin Wang, Xiangmei Li, Xing Shen, Ang Zhang, Jinxiu Liu, Hongtao Lei
Summary: An immunochromatographic assay based on PMs was developed for sensitive and quantitative detection of AFB(1) in maize. The method showed good accuracy in both PB and maize samples, and is a simple and rapid point-of-care testing method.
Review
Chemistry, Applied
Qi Chen, Meng Meng, Wang Li, Ying Xiong, Yong Fang, Qinlu Lin
Summary: This review highlights the recent advances in immunosensors, aptasensors, and other emerging receptor-based sensors for the detection of AFM(1) in milk and dairy products. It discusses their unique advantages, drawbacks, and future opportunities and challenges.
Article
Food Science & Technology
Nysrine Mannani, Ahmed Tabarani, Chakib El Adlouni, El Hassane Abdennebi, Abdellah Zinedine
Summary: This study assessed AFM1 levels in milk samples from four Moroccan cities in 2019, finding that 13.4% of samples were contaminated, with over half having AFM1 levels below the detection limit. The estimated exposure level of Moroccan adults to AFM1 from milk was higher than dietary intakes estimated by JECFA for Middle Eastern and African diets.
Article
Chemistry, Analytical
Michailia Angelopoulou, Dimitra Kourti, Konstantinos Misiakos, Anastasios Economou, Panagiota Petrou, Sotirios Kakabakos
Summary: A silicon-based optoelectronic immunosensor for the detection of AFM1 in milk, chocolate milk, and yogurt has been developed. The immunosensor consists of ten Mach-Zehnder silicon nitride waveguide interferometers (MZIs) integrated on the same chip with the respective light sources. The detection method is based on a three-step competitive immunoassay and achieves accurate on-site AFM1 determination in milk samples.
Article
Chemistry, Applied
Xiaoqian Tang, Gaelle Catanante, Xiaorong Huang, Jean-Louis Marty, Hong Wang, Qi Zhang, Peiwu Li
Summary: This study successfully devised a nontoxic electrochemical immunosensor for the quantitative determination of aflatoxin M-1. The immunosensor demonstrated good selectivity and sensitivity, making it suitable for practical applications such as milk samples.
Article
Food Science & Technology
Gamal M. M. Hamad, Hussein S. Abo El-Makarem, Marwa G. G. Allam, Osama S. S. El Okle, Marwa I. I. El-Toukhy, Taha Mehany, Yasser El-Halmouch, Mukhtar M. F. Abushaala, Mohamed S. S. Saad, Sameh A. A. Korma, Salam A. A. Ibrahim, Elsayed E. E. Hafez, Amr Amer, Eman Ali
Summary: The aim of this research was to investigate the ability of bentonite to adsorb and/or eliminate aflatoxin M-1 (AFM(1)) from different types of raw milk. The study found that goat milk had the highest AFM(1) value compared to other milks. The adsorption of AFM(1) by bentonite was found to be concentration-dependent and reached maximum levels after 12 hours. Bentonite HAFR 3 exhibited the highest adsorption efficiency and resulted in the lowest residual levels of AFM(1) in both phosphate buffer saline and milk. Furthermore, the use of bentonite led to changes in the nutritional characteristics of milk.
Review
Chemistry, Analytical
Fabian Thurner, Fatima AlZahra'a Alatraktchi
Summary: Aflatoxin, a mycotoxin produced by molds, poses a serious health risk for humans and animals. It enters the food chain through various routes, including contaminated animal feed into milk. Rapid and reliable detection methods for aflatoxin in milk are crucial, and electrochemical biosensors, specifically immunosensors and aptasensors, offer promising solutions.
MICROCHEMICAL JOURNAL
(2023)
Article
Engineering, Electrical & Electronic
Kuter Erdil, O. Gokalp Akcan, Ozguer Gul, Y. Daghan Gokdel
Summary: In this study, a paper-based perforated disposable biosensing device is proposed for the detection of aflatoxin M1 molecules. The system utilizes a competitive assay method and low-cost materials, and enhances sensitivity with closely spaced perforations and magnetic nanoparticles. Experimental results show that the system can detect a minimum of 14μg of AFM1 molecules.
SENSORS AND ACTUATORS A-PHYSICAL
(2022)
Article
Biochemical Research Methods
Yiyi Chen, Yanghong Shen, Haifeng Wang, Jinzhi Zhang, Junli Zhu
Summary: Due to universal contamination and synergistic toxicity of multiple mycotoxins in foodstuff, a novel dual-channel immunochromatographic assay (ICA) based on improved up-conversion nanoparticles (IUCNPs) was developed for rapidly detecting aflatoxin B1 (AFB1) and zearalenone (ZEN). The IUCNPs-ICA exhibited high specificity for the targeted mycotoxins, and showed excellent accuracy with low detection limits. The method also demonstrated good stability and precision during long-term storage, suggesting potential for on-site screening in agricultural products.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Gang Qu, Yuxin Zhao, Qiaoli Zhang, Jina Wu, Xiaosen Li, Yang Yang, Shilei Liu
Summary: In this study, magnetic mesoporous materials combined with real-time in situ mass spectrometry were used for the high-throughput detection of hydrolyzed products of organophosphorus nerve agents. The method showed good linearity, low limits of detection and quantification, and high extraction recoveries. The magnetic preparation method used was quick, cost-effective, rugged, and safe. The results demonstrated the potential of this method for rapid and efficient determination of the target analytes in environmental samples.
ANALYTICAL METHODS
(2024)
Article
Chemistry, Analytical
Anna Hildebrand, Mariam Merchant, Danny O'Hare
Summary: Substandard and falsified artemisinin derivatives in antimalarials have caused significant deaths and economic losses. This study evaluates the feasibility of voltammetric methods for identifying and quantifying artemether. The findings suggest that electrochemical analysis shows promise as a method for artemether identification and quantification.
ANALYTICAL METHODS
(2024)
Article
Chemistry, Analytical
Marx Osorio Araujo Pereira, Alvaro Ferreira Junior, Edson Silvio Batista Rodrigues, Helena Mulser, Giovanna Nascimento de Mello e Silva, Wallans Torres Pio dos Santos, Eric de Souza Gil
Summary: Brazilian spotted fever (BSF) is a serious and rapidly evolving disease. A new impedimetric immunosensor was developed for rapid diagnosis by measuring specific antibodies in plasma. The sensor demonstrated selectivity and accuracy, and has potential for important applications in diagnostic testing.
ANALYTICAL METHODS
(2024)
Article
Chemistry, Analytical
Kathrin Schilling, Ronald A. Glabonjat, Olgica Balac, Marta Galvez-Fernandez, Arce Domingo-Relloso, Vesna Slavkovich, Jeff Goldsmith, Miranda R. Jones, Tiffany R. Sanchez, Ana Navas-Acien
Summary: Analysis of trace elements in urine is an important tool for assessing exposures, diagnosing nutritional status, and guiding public health and healthcare intervention. This study provides a sensitive method for analyzing 18 elements in urine samples, using only 100 μL. The results show good accuracy and sensitivity of the method.
ANALYTICAL METHODS
(2024)
Article
Chemistry, Analytical
Mengya Li, Shijie Liu, Shiliang Guo, Dong Liang, Miaoyun Li, Yaodi Zhu, Lijun Zhao, Jong-Hoon Lee, Gaiming Zhao, Yangyang Ma, Yanxia Liu
Summary: In this study, a magnetic flow device was developed to purify spores in a culture medium system. The device used magnetic nanoparticles to absorb vegetative cells, separating them from the spores. The achieved purity of the collected spores was over 95%. The study also demonstrated a rapid quantitative detection method using Raman spectroscopy.
ANALYTICAL METHODS
(2024)
Article
Chemistry, Analytical
Wanqiong Liu, Zixuan Wu, Jianwei Peng, Zebin Xu, Yong Liang
Summary: Metal-organic frameworks (MOFs) are effective carriers for molecular imprinting, but their poor dispersibility in aqueous solution is a significant drawback. In this study, we have applied amphiphilic block copolymers and molecularly imprinted technology on MOFs to improve the hydrophilicity of molecularly imprinted fluorescent materials.
ANALYTICAL METHODS
(2024)