Article
Chemistry, Analytical
Chong Cai, Qi Zhang, Seyni Nidiaye, Honglin Yan, Wen Zhang, Xiaoqian Tang, Peiwu Li
Summary: An anti-idiotypic nanobody VHH C4 was developed and showed good specificity and sensitivity in detecting aflatoxin M-1, serving as a promising alternative antigen in immunological methods. A competitive ELISA method was used for detection, and its potential application in dairy products was validated.
MICROCHEMICAL JOURNAL
(2021)
Article
Environmental Sciences
Arpana Parihar, Nishant Kumar Choudhary, Palak Sharma, Raju Khan
Summary: Detection of food toxins requires sensitive and selective techniques to prevent economic loss. Nanostructured material-enabled biosensors have been developed to improve the detection of food toxins, particularly aflatoxin. Biosensor-based detection of aflatoxin is quick, affordable, and requires less skilled personnel compared to conventional techniques. MXenes and aptamers are promising elements for biosensing, offering biocompatibility, mechanical strength, selectivity, and ease of synthesis. This review explores the latest aptamer-based MXene-enabled biosensing technologies for detecting mycotoxins in food and environmental samples, discussing synthesis processes, computational approaches, and future potential. The advantages of aptasensors over conventional methods are highlighted, along with their limitations. The review emphasizes the benefits of next-generation MXene-enabled biosensing technologies in terms of simplicity, sensitivity, and selectivity, as well as their economic/commercial potential. Challenges and a roadmap for developing advanced biosensing technologies are also discussed.
ENVIRONMENTAL POLLUTION
(2023)
Article
Chemistry, Analytical
Shuofeng Li, Fangfang Wang, Bin Zhao, Chun Wang, Zhi Wang, Qiuhua Wu
Summary: In this study, a colorimetric and fluorescent dual-mode aptasensor based on MnO2 nanoflowers was developed for accurate and sensitive detection of AFB1. The proposed method showed promising results in the determination of AFB1 in milk samples and exhibited high detection sensitivity and accuracy.
ANALYTICA CHIMICA ACTA
(2023)
Article
Mycology
D. Costamagna, M. Gaggiotti, M. L. Signorini
Summary: A quantitative risk assessment was conducted on exposure to aflatoxin M1 (AFM(1)) in milk and dairy products in Argentina. The study found that while certain populations may face higher risks of exposure, overall the impact on the health of infants, toddlers, and children is not significant.
MYCOTOXIN RESEARCH
(2021)
Article
Chemistry, Multidisciplinary
Qinqin Qiao, Xiaodong Guo, Fang Wen, Lu Chen, Qingbiao Xu, Nan Zheng, Jianbo Cheng, Xiuheng Xue, Jiaqi Wang
Summary: A fast and simple method for detecting AFM(1) based on a structure-switching signaling aptamer was developed in this study. This aptasensor can quantitatively monitor AFM(1) concentrations by changes in fluorescence signal and was applied successfully to milk samples for high-throughput and quantitative determination of mycotoxin levels in dairy products.
FRONTIERS IN CHEMISTRY
(2021)
Article
Chemistry, Applied
Guangming Li, Chen Liu, Xingcai Zhang, Pengjie Luo, Guimiao Lin, Wenxiao Jiang
Summary: A facile hydrothermal method was used to synthesize highly photoluminescent N-doped carbon dots with quantum yields reaching 97.1%. An label-free immunosensor based on the inner filter effect of carbon dots was developed for ultrasensitive detection of aflatoxin M1 residues in milk, with a detection limit of 0.0186 ng/mL. The immunosensor showed good linear relationship, high recoveries, and low relative standard deviations, making it a reliable on-site screening method for aflatoxin M1 residue analysis.
Article
Food Science & Technology
Abu Hasan Sumon, Farjana Islam, Nayan Chandra Mohanto, Rahanuma Raihanu Kathak, Noyan Hossain Molla, Sohel Rana, Gisela H. Degen, Nurshad Ali
Summary: The study revealed that 78.6% of milk and dairy products in Bangladesh were contaminated with AFM(1) in concentrations ranging from 5.0 to 198.7 ng/L. However, yogurt and milk powder samples had lower levels of AFM(1) below the EU regulatory limit for milk. This highlights the need for further monitoring of aflatoxin contamination in milk and dairy products in Bangladesh.
Article
Chemistry, Applied
Seyedeh Fatemeh Ahmadi, Mohammad Hojjatoleslamy, Hossein Kiani, Hooman Molavi
Summary: In this study, a label-free electrochemical aptasensor was developed for accurate and rapid detection of Aflatoxin M1. By optimizing aptamer immobilization time, concentration, and binding time, the sensor showed a linear range and limit of detection. Compared to traditional HPLC methods, this sensor offers a faster and more accurate way to detect Aflatoxin M1.
Article
Agriculture, Dairy & Animal Science
Magdalena Stobiecka, Jolanta Krol, Aneta Brodziak
Summary: Consumption of food products rich in natural antioxidants improves the antioxidant status and reduces the risk of civilization diseases; milk and dairy products are rich sources of antioxidant compounds that can be enhanced through animal nutrition or processing modifications.
Article
Food Science & Technology
Marzieh Hajmohammadi, Reza Valizadeh, Abassali Naserian, Mohammad E. Nourozi, Carlos A. F. Oliveira
Summary: The study found that using raw mineral clays with appropriate particle sizes can effectively reduce the content of AFM(1) in dairy cow milk, with a reduction effect equivalent to commercial adsorbents. This method can provide a more economical alternative to reduce the transfer rate of AFM(1) to dairy cow milk.
INTERNATIONAL JOURNAL OF DAIRY TECHNOLOGY
(2021)
Review
Chemistry, Analytical
Xiaoqian Tang, Jiasi Zuo, Cheng Yang, Jun Jiang, Qi Zhang, Jianfeng Ping, Peiwu Li
Summary: Biotoxins, including mycotoxins, marine toxins, and bacterial food toxins, pose significant threats in various fields such as food, feed, seafood, and medicine due to their potential harmful effects on humans. The increased consumption of fast food and prepared food without considering associated toxins is a critical issue. Therefore, the development of effective methods for monitoring biotoxins is crucial. In recent years, biosensors have been widely used for biotoxin detection due to their simplicity, quick detection procedure, low cost, and other advantages. This study provides a comprehensive overview of the application of biosensors in biotoxin detection, focusing on optical, electrochemical, piezoelectric, and photothermal applications. Additionally, the integration of different detection platforms as multimodal combination biosensors is discussed to meet the demand for highly accurate technologies. Major challenges faced by biosensors are examined, and novel insights for improving food safety technology are provided.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Review
Biochemistry & Molecular Biology
Ruben Agregan, Noemi Echegaray, Maria Lopez-Pedrouso, Radwan Kharabsheh, Daniel Franco, Jose M. Lorenzo
Summary: Proteomics has made significant advances in the field of medicine and has applications in other scientific areas, such as the study of milk. Milk proteomics can help characterize milk samples and products in detail, with significant applications in industry for quality control and improvement.
Article
Chemistry, Applied
Harpreet Singh, Shalini Singh, Sanjeev K. Bhardwaj, Gurjeet Kaur, Madhu Khatri, Akash Deep, Neha Bhardwaj
Summary: In this research, a novel fluorescent immunosensor based on nitrogen-doped carbon quantum dots has been developed for the analysis of aflatoxin M1 in dairy products. The sensor exhibited high sensitivity and low limit of detection, and was further developed as a lateral flow design for on-site detection.
Article
Food Science & Technology
I Salazar, I Lopez, P. Glorio-Paulet, C. Gomez
Summary: Limited research on aflatoxin contamination in Peru focused on detecting AFB(1) and AFM(1) levels, with a study conducted in a typical intensive dairy farm in Northern Peru revealing seasonal variations. The study found that December had higher levels of AFM(1) contamination in milk and AFB(1) in feedstuffs. Commonly used feedstuffs like maize, soybean meal, and whole soybean showed varying levels of AFB(1) contamination.
WORLD MYCOTOXIN JOURNAL
(2021)
Review
Food Science & Technology
Yun Jiang, Ibukun M. Ogunade, Diwakar Vyas, Adegbola T. Adesogan
Summary: Aflatoxins, produced by fungi, are toxic and carcinogenic substances that can contaminate livestock feeds and transfer to milk, posing threats to animal and human health as well as the safety of dairy products. Effective strategies are needed to reduce aflatoxin contamination and prevent toxin transfer for improving production profitability and safeguarding health.
Article
Chemistry, Analytical
Qi Chen, Di Li, Amirreza Malekanfard, Quanliang Cao, Jianhan Lin, Maohua Wang, Xiaotao Han, Xiangchun Xuan
ANALYTICAL CHEMISTRY
(2018)
Article
Chemistry, Analytical
Qi Chen, Fengchun Huang, Gaozhe Cai, Maohua Wang, Jianhan Lin
SENSORS AND ACTUATORS B-CHEMICAL
(2018)
Article
Chemistry, Analytical
Xiaoting Huo, Qi Chen, Lei Wang, Gaozhe Cai, Wuzhen Qi, Zengzilu Xia, Weijia Wen, Jianhan Lin
Article
Biochemistry & Molecular Biology
Xuerou Wang, Qi Chen, Zhichang Sun, Yidan Wang, Benchao Su, Chenghui Zhang, Hongmei Cao, Xing Liu
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2020)
Article
Chemistry, Analytical
Jing Hu, Shu Zhou, Lifeng Zeng, Qi Chen, Hong Duan, Xirui Chen, Xiangmin Li, Yonghua Xiong
Summary: The study synthesized hydrazide and carboxylic group modified quantum dot beads and compared two coupling strategies for preparing QB probes systematically. Results showed that the QB probe by HDZ-O method had a shorter reaction time and optimal labeled antibody content compared to the carbodiimide method, with higher bio-activity. The QB probe by HDZ-O method also showed better detection performance in a sandwich ICA for hepatitis B surface antigen detection.
Article
Food Science & Technology
Zhen Zhang, Yuhao Wu, Qi Chen, Xiaolin Huang, Yonghua Xiong
Summary: In this work, a novel hyperbranched Au plasmonic blackbody (AuPB) was synthesized and applied as a signal amplification label in an immunochromatographic test strip (ICTS) sensor for the sensitive detection of aflatoxin B-1 (AFB(1)) in maize. The AuPB-ICTS sensor showed significantly lower detection limit and improved accuracy compared to traditional ICTS sensor. This method can serve as an alternative rapid diagnostic approach for sensitive and reliable screening of AFB(1) in maize.
FOOD ANALYTICAL METHODS
(2021)
Article
Food Science & Technology
Kunlu Bao, Xing Liu, Qi Xu, Bencao Su, Zilong Liu, Hongmei Cao, Qi Chen
Summary: In this study, a heptamer fusion protein was developed by fusing the OTA-specific nanobody with the C4-binding protein, which was utilized to enhance the sensitivity of detecting OTA in coffee samples using a competitive ELISA. The sensitivity enhancement strategy resulted in a significantly lower IC50 and LOD compared to traditional monomer-based ELISA, demonstrating the potential of nanobody multimerization in enhancing analytical signals in immunoassays.
Article
Food Science & Technology
Kunlu Bao, Xing Liu, Yujing Liao, Zilong Liu, Hongmei Cao, Long Wu, Qi Chen
Summary: "Ochratoxin A (OTA), a well-known mycotoxin, poses potential toxic effects on food safety in humans and animals. In this study, a one-step bioluminescent enzyme-linked immunosorbent assay (BLEIA) using a nanoluciferase-nanobody fusion protein (Nb28-Nluc) was developed for the detection of OTA in coffee samples. The BLEIA method showed advantages of simplicity, rapidity, and high sensitivity compared to conventional enzyme-linked immunosorbent assay. It also exhibited good repeatability and accuracy in spike recovery experiments. Thus, the proposed one-step BLEIA holds great potential for sensitive and accurate screening of OTA in food samples."
Article
Chemistry, Analytical
Zilong Liu, Xing Liu, Hua Pei, Kunlu Bao, Benchao Su, Hongmei Cao, Long Wu, Qi Chen
Summary: We developed a convenient method for the fabrication of homogeneous multivalent primary antibody-enzyme complexes (PAECs) using protein self-assembly and validated it using anti-alpha-fetoprotein nanobody and alkaline phosphatase as models. The heptavalent PAECs showed a 4-fold enhancement in enzymatic catalytic activity compared to monovalent PAECs. The developed heptavalent PAEC-based ELISA demonstrated a detection limit of 0.69 ng mL(-1), about 3 times higher than that of monovalent PAECs, with the entire detection process completed within 3 hours.
ANALYTICAL METHODS
(2023)
Article
Chemistry, Multidisciplinary
Qi Chen, Yuanyuan Wang, Fujing Mao, Benchao Su, Kunlu Bao, Zeling Zhang, Guifang Xie, Xing Liu
Article
Chemistry, Analytical
Benchao Su, Yidan Wang, Hua Pei, Zhichang Sun, Hongmei Cao, Chenghui Zhang, Qi Chen, Xing Liu
ANALYTICAL METHODS
(2020)
Review
Chemistry, Multidisciplinary
Qi Chen, Zhenyun He, Fujing Mao, Hua Pei, Hongmei Cao, Xing Liu
Article
Chemistry, Multidisciplinary
Qi Chen, Danyang Sun, Hua Pei, Benchao Su, Kunlu Bao, Hongmei Cao, Chenghui Zhang, Bruce D. Hammock, Xing Liu
Article
Biochemical Research Methods
Qi Chen, Di Li, Jessica Zielinski, Lukasz Kozubowski, Jianhan Lin, Maohua Wang, Xiangchun Xuan
Article
Chemistry, Applied
M. Graca Dias, Elsa Vasco, Francisco Ravasco, Lufsa Oliveira
Summary: This study estimated the vitamin D intake of "adults" and "elderly" populations in Portugal using the TDS methodology. The results showed that the majority of people had inadequate vitamin D intake, well below the Dietary Reference Values.
Article
Chemistry, Applied
Yanan Wang, Jiachen Shi, Yong-Jiang Xu, Chin-Ping Tan, Yuanfa Liu
Summary: This study investigates the variations in lipid digestion profiles among individuals of different ages using in vitro digestion models. The findings suggest that adults have a more comprehensive lipid digestion compared to infants, and infants tend to release shorter chain length and more saturated free fatty acids during digestion. Additionally, the particle sizes in the stomach of the elderly were consistently larger. This study enhances our understanding of how lipids with different degrees of unsaturation undergo digestion in diverse age groups.
Article
Chemistry, Applied
Hyunjong Yu, Huisu Kim, Pahn-Shick Chang
Summary: Chitosan-pectin emulsion-filled hydrogel (EFH) was developed to enhance the bioaccessibility of lipophilic bioactive compounds through intestinal delivery. The EFH, prepared without crosslinking agents, demonstrated improved mechanical strength and compactness with higher pectin concentration. It retained the emulsion at pH 2.0 and released it at pH 7.4, resulting in enhanced release of free fatty acids and improved bioaccessibility of curcumin.
Article
Chemistry, Applied
Tongze Zhang, Siqi Hong, Jia-Rong Zhang, Pin-He Liu, Siyi Li, Zixian Wen, Jianwei Xiao, Guirong Zhang, Olivier Habimana, Nagendra P. Shah, Zhongquan Sui, Harold Corke
Summary: Lactic acid fermentation significantly affects the morphology and physicochemical properties of proso millet starch, including the formation of surface indentations and small pores, decrease in gelatinization temperatures, and changes in hardness and adhesiveness.
Article
Chemistry, Applied
Liqin Kong, Feng Hong, Peng Luan, Yiping Chen, Yaoze Feng, Ming Zhu
Summary: This study presents a novel impedance biosensor using composite nanomaterials and T1R1 as a signal probe, which can competitively and ultra-sensitively detect umami intensity. The biosensor exhibits exceptional analytical performance and is suitable for food flavor evaluation.
Article
Chemistry, Applied
Kunying Lv, Yixin Yang, Qilong Li, Ran Chen, Liang Deng, Yiwei Zhang, Ning Jiang
Summary: Horse's milk, with its high nutritional value and low allergenic proteins, could be a substitute for cow's milk in infant consumption. A proteomic method was used to identify and compare milk fat globule membrane (MFGM) and whey proteins from different horse breeds. The study found differences in protein composition and functionality, which could support the development of formulas more suitable for human infants.
Article
Chemistry, Applied
Enrique Jacobo Diaz-Montana, Helene Brignot, Ramon Aparicio-Ruiz, Thierry Thomas- Danguin, Maria Teresa Morales
Summary: Sensory perception of virgin olive oil is influenced by phenols and volatiles, which are affected by the composition of the oil and biological factors. This study investigated the effect of saliva and phenols on the release of volatiles, and found that the presence of phenols decreased the release of saturated volatiles.
Article
Chemistry, Applied
Wei Zhou, Rui Zhang, Zhen Cai, Fangfang Wu, Yong Hu, Chao Huang, Kun Hu, Yun Chen
Summary: Environmentally friendly and outstanding pH-responsive cationic starch nanoparticles (CSNP) were prepared from pH-sensitive starch. CSNP exhibited nanosize and regular sphere, highly free-flowing molecular chains, and demonstrated excellent pH responsiveness through multiple emulsion/demulsification transitions.
Article
Chemistry, Applied
Andrea Koo, Vinayak Ghate, Weibiao Zhou
Summary: This study suggests that direct seeding may negatively affect the nutritional quality of crops, causing a decrease in ascorbic acid, vitamin K, and total glucosinolate content.
Article
Chemistry, Applied
Tonghao Du, Yazhou Xu, Xiaoyan Xu, Shijin Xiong, Linli Zhang, Biao Dong, Jinqing Huang, Tao Huang, Muyan Xiao, Tao Xiong, Mingyong Xie
Summary: This study successfully improved the ACE inhibitory activity of black sesame seeds by fermenting them with Lactobacillus Plantarum NCU116 and hydrolyzing them using acid protease. The RF-PSO model was used to predict the ACE inhibitory activity during the hydrolysis process. Eight peptides with ACE inhibitory activity were identified from fermented black sesame seed hydrolysates after separation and screening.
Article
Chemistry, Applied
Yao Liu, Songyi Lin, Kexin Liu, Shan Wang, Qiaozhen Liu, Na Sun
Summary: This study analyzed the structural changes of shrimp proteins during digestion, predicted the immunodominant epitopes, and validated their allergenicity. The results showed that shrimp proteins were degraded into peptides during digestion, but still carried IgE epitopes that trigger allergic reactions.
Article
Chemistry, Applied
Tiantian Fu, Hongwei Cao, Yu Zhang, Xiao Guan
Summary: This study investigates the impact of milling on the active components in rice, with a focus on the stability and bioaccessibility of phenols, VB1, and alpha-GABA during cooking and digestion. The findings show that milling exacerbates the instability of gamma-GABA during cooking and VB1 during digestion, and it affects the bioaccessibility of these active compounds.
Article
Chemistry, Applied
Zhihao Yang, Yanru Hou, Min Zhang, Puxin Hou, Chang Liu, Lu Dou, Xiaoyu Chen, Lihua Zhao, Lin Su, Ye Jin
Summary: This study investigated the molecular mechanism of feeding regimes on lamb flavor by using TMT labeling combined with MS. The results showed that pasture-fed groups had higher levels of amino acids and volatile flavor substances compared to concentrate-fed groups. Additionally, several differentially abundant proteins associated with lamb flavor were identified.
Article
Chemistry, Applied
Zixuan Xie, De Zhang, Junyu Zhu, Qianqian Luo, Jun Liu, Jingtao Zhou, Xiaoyong Wang, Yuqiong Chen, Zhi Yu, Dejiang Ni
Summary: This study investigated the acidification of aroma-enhanced black tea during storage. Analysis of non-volatile substances and organic acids using UPLC-Q-TOF/MS and HPLC revealed a decrease in soluble sugars and amino acids, while an increase in organic acids such as oxalic acid, malic acid, and quinic acid. In vitro experiments further demonstrated that the acidification is a result of the decomposition of sugars and amino acids by heating, as well as the oxidation of aromatic aldehydes. Additionally, the study showed that the taste composition of tea infusion is altered, with reduced amino acids, catechins, soluble sugars, and flavonoids. This research provides a theoretical basis for improving the quality of black tea.
Article
Chemistry, Applied
Lin Wang, Falai Ma, Zihan Li, Yan Zhang
Summary: This study developed time-temperature integrators based on amyloglucosidase@Cu3(PO4)2 nanoflowers for monitoring the freshness of chilled pork. The results showed that the integrators were highly reliable and accurate in predicting the quality of chilled pork.
