Article
Biochemistry & Molecular Biology
Nur Diyana Jamaluddin, Nur-Fadhilah Mazlan, Ling Ling Tan, Nurul Yuziana Mohd Yusof, Bahariah Khalid
Summary: In this study, an acrylic micropolymer-based RNA biosensor was developed to detect viral RNA using a G-quadruplex DNA probe. The biosensor showed a rapid response time and high sensitivity, allowing real-time detection of DENV2 in non-invasive human fluid samples.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Letter
Immunology
Marta Giovanetti, Luiz Augusto Pereira, Gilberto A. Santiago, Vagner Fonseca, Maria Paquita Garcia Mendoza, Carla de Oliveira, Laise de Moraes, Joilson Xavier, Stephane Tosta, Hegger Fristch, Emerson de Castro Barbosa, Evandra Strazza Rodrigues, Dana Figueroa-Romero, Carlos Padilla-Rojas, Omar Caceres-Rey, Ana Flavia Mendonca, Fernanda de Bruycker Nogueira, Rivaldo Venancio da Cunha, Ana Maria Bispo de Filippis, Carla Freitas, Cassio Roberto Leonel Peterka, Carlos Frederico Campelo de Albuquerque, Leticia Franco, Jairo Andres Mendez Rico, Jorge L. Munoz-Jordan, Vinicius Lemes da Silva, Luiz Carlos Junior Alcantara
Summary: In this study, a cosmopolitan genotype of dengue virus serotype 2 was identified in a 56-year-old male patient from the state of Goias in Brazil using nanopore sequencing and phylogenetic analysis. The emergence of this genotype in Brazil highlights the need for risk assessment and surveillance to mitigate its epidemic potential.
EMERGING INFECTIOUS DISEASES
(2022)
Article
Chemistry, Multidisciplinary
Poulomi Biswas, Giri Nandagopal Mukunthan Sulochana, Theneyur Narayanaswamy Banuprasad, Pankaj Goyal, Dolonchampa Modak, Ananta Kumar Ghosh, Suman Chakraborty
Summary: A multiplex paper/polymer-based detection strip has been developed for early detection of all four serotypes of dengue viruses, which can facilitate disease management and patient recovery.
Article
Virology
Priya Verma, Sayan Banerjee, Upasana Baskey, Supradip Dutta, Sagnik Bakshi, Raina Das, Sandip Samanta, Shanta Dutta, Provash C. Sadhukhan
Summary: This study aims to investigate the circulating dengue serotypes and their clinicopathological associations among pediatric patients in Kolkata, India. It was found that all four serotypes co-circulated, with DENV2 being the major strain. The study also observed changes in trends of clinical manifestations and liver function profiles during different phases of dengue fever.
JOURNAL OF MEDICAL VIROLOGY
(2022)
Article
Virology
Hirawati Deval, Sthita P. Behera, Awdhesh Agrawal, Rajeev Singh, Brijranjan Misra, Vanka Janardhan, Gajanan Patil, Kamlesh Sah, Niraj Kumar, Ravishankar Singh, Vijay P. Bondre
Summary: During the dengue outbreaks in 2015 to 2016 in Uttar Pradesh and Bihar, only DEN-2 serotype was prevalent, with DV-2 being the main circulating serotype. Genetic analysis revealed the presence of lineages I and III of DV-2 cosmopolitan genotype in 2015, and lineage II in 2016, based on full-length envelope (E) protein sequence derived from patient's sera and DV isolates. The phylogenetic analysis showed a genetic relationship between these DV-2 strains and recent DV-2 genotypes reported in DEN outbreaks in north India, indicating the circulation of cosmopolitan genotype of DV-2 in eastern Uttar Pradesh and western Bihar.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Article
Biochemistry & Molecular Biology
Ken-Ichiro Matsunaga, Michiko Kimoto, Vanessa Weixun Lim, Hui Pen Tan, Yu Qian Wong, William Sun, Shawn Vasoo, Yee Sin Leo, Ichiro Hirao
Summary: Expanding the genetic alphabet of DNA with unnatural bases significantly enhances the affinity of DNA aptamers for target proteins, resulting in high-specificity UB-DNA aptamers; these high-affinity UB-DNA aptamers demonstrate remarkable target specificity, capable of identifying subtle variants of DEN-NS1 and enabling precise diagnoses of diseases.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Virology
Maria Celeste Torres, Marcos Cesar Lima de Mendonca, Cintia Damasceno dos Santos Rodrigues, Vagner Fonseca, Mario Sergio Ribeiro, Ana Paula Brandao, Rivaldo Venancio da Cunha, Ana Isabel Dias, Lucy Santos Vilas Boas, Alvina Clara Felix, Maira Alves Pereira, Luzia Maria de Oliveira Pinto, Anavaj Sakuntabhai, Ana Maria Bispo de Filippis
Summary: The study found significant differences in viral population structure among different clinical classifications of Dengue virus serotype 2 circulating in Brazil, driven mainly by different infection times and selection pressures rather than clinical outcomes. However, diversity in the NS2B gene was found to be constrained, regardless of clinical outcome and infection time. High frequency of non-synonymous intrahost single-nucleotide variants were identified among cases with warning signs and severe cases, but no single variant appeared to characterize cases of greater severity independently.
Article
Virology
Lijuan Qiu, Xinwei Huang, Jia Luo, Yujiao Zhao, Shan Hong, Xiaodan Wang, Kai Feng, Yue Pan, Qiangming Sun
Summary: The study investigates the risk of cross infection and severity of dengue virus in BALB/c mice, particularly focusing on the impact of maternal immunity and heterotypic infection. The results suggest that maternal immunity enhances subsequent heterotypic infection and can lead to severe disease. This study has implications for understanding cross infection and vaccine development.
JOURNAL OF MEDICAL VIROLOGY
(2022)
Article
Multidisciplinary Sciences
Soumya P. Tripathy, Manvitha Ponnapati, Suhaas Bhat, Joseph Jacobson, Pranam Chatterjee
Summary: In this study, computational protein modeling tools were used to propose a molecular beacon architecture for high-sensitivity detection of the SARS-CoV-2 spike protein receptor binding domain. By integrating these beacons on a miniaturized total internal reflection fluorescence microscope, the SARS-CoV-2 and pseudotyped SARS-CoV-2 could be detected with limits of detection in the femtomolar range.
Article
Microbiology
Hua Sun, Wenwu Yao, Abubakar Siddique, Fan He, Min Yue
Summary: This study aimed to investigate the genetic diversity and molecular evolution of the dengue virus in Hangzhou, China, during and after a dengue fever epidemic. The results showed that the virus strains from 2017 were imported from Singapore, Malaysia, and Thailand, while local strains from 2018 and 2019 were closely associated with Guangzhou isolates, indicating within-country transmission. Various amino acid substitutions and recombination events were observed.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Immunology
Su-Jhen Hung, Huey-Pin Tsai, Ya-Fang Wang, Wen-Chien Ko, Jen-Ren Wang, Sheng-Wen Huang
Summary: This study investigates the association between the detected frequencies/times of SNVs/DVGs in dengue patients and severe disease. The results show that certain SNVs and DVGs are significantly different between patients with dengue fever and those with severe dengue, and a machine learning model can successfully discriminate severe patients. These findings expand our understanding of the intrahost viral population in dengue pathogenesis.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Biophysics
Rimsha B. Jamal, Toni Vitasovic, Ulrich Gosewinkel, Elena E. Ferapontova
Summary: In this study, a sensitive and simple electrocatalytic amplification method for detecting Escherichia coli 23S ribosomal rRNA has been developed. The RNA sample was cleaved by RNase H enzyme, and then hybridized with a methylene-blue (MB) - labelled DNA probe. The formed duplex acted as an electrical wire for electron transfer, enabling the electrocatalytic reduction on the modified electrodes. This assay allowed the detection of synthetic E. coli DNA and 23S rRNA with a limit of 1 fM.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Infectious Diseases
Esther Annan, Jailos Lubinda, Jesus Trevino, William Messer, Dina Fonseca, Penghua Wang, Jurgen Pilz, Benjamin Lintner, Aracely Angulo-Molina, Ana L. Gallego-Hernandez, Ubydul Haque
Summary: Pathogen strain diversity plays a crucial role in epidemic trajectories. In this study, the impact of bioclimatic factors on the spatial distribution of dengue virus serotypes was examined in Mexico. Municipality-level environmental suitability maps were developed using climatic variables and maximum entropy modeling. The results highlighted the importance of temperature, precipitation, and humidity in determining the suitability for different DENV serotypes. Ongoing surveillance and monitoring of bioclimatic parameters are recommended to track potential changes in DENV serotype distribution.
TRANSBOUNDARY AND EMERGING DISEASES
(2023)
Article
Immunology
Yu-Ching Dai, Ava Kristy Sy, Mario Jiz, Jih-Jin Tsai, Joan Bato, Mary Ann Quinones, Mary Anne Joy Reyes, Wei-Kung Wang
Summary: It is important to develop an assay that can identify the baseline DENV serostatus of Dengvaxia recipients. The University of Hawaii's DENV1-4 NS1 IgG ELISA showed high sensitivity and specificity in determining DENV serostatus and has implications for assessing the long-term safety and effectiveness of Dengvaxia.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Virology
Mya Myat Ngwe Tun, Kishor Pandey, Takeshi Nabeshima, Aung Kyaw Kyaw, Mandira Adhikari, Sandra Kendra Raini, Shingo Inoue, Shyam Prakash Dumre, Basu Dev Pandey, Kouichi Morita
Summary: Dengue virus (DENV) is increasingly prevalent in Nepal, with the most common serotype being DENV-2. Genomic analysis showed multiple amino acid substitutions in the E, NS3, and NS5 genes, and DENV-2 isolates in Nepal were classified into Asian and Indian clades. Further research on different DENV serotypes is needed to understand the epidemics in Nepal.
Article
Biochemistry & Molecular Biology
Jirayu Boonyakida, Indra Memdi Khoris, Fahmida Nasrin, Enoch Y. Park
Summary: This study aimed to develop a modular display platform for protein decoration on norovirus-like particles (NoV-LPs) by combining the NoVLP scaffold with the SpyTag/SpyCatcher bioconjugation system. Results showed that adding an extension linker increased the conjugation efficiency and level of protein decoration on the NoV-LPs. This study provides a strategy for enhancing the conjugation efficiency of NoV-LP and demonstrates the platform's utility for developing vaccines or functional nanoparticles.
Article
Engineering, Environmental
Manh Dung Nguyen, Thanh Binh Nguyen, Linh Hai Tran, Thuy Giang Nguyen, Is Fatimah, Eko Prasetyo Kuncoro, Ruey-An Doong
Summary: In this study, flower-like core-shell CNBS@MnO2 heterojunction with Z-scheme structure was successfully constructed for enhanced photocatalytic degradation of diclofenac. CNBS@MnO2 provided large specific surface area and reduced interfacial resistance for rapid electron transport, and the redox cycling of Mn species activated PMS to produce sulfate radicals. The heterojunction exhibited excellent performance and stability, and the dominant reactive species for diclofenac degradation were identified.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Chemistry, Analytical
Akhilesh Babu Ganganboina, Indra Memdi Khoris, Akinori Konno, Tian-Cheng Li, Akihiro Okamoto, Enoch Y. Park
Summary: This article describes the design and construction of a visible light-driven photoelectrochemical (PEC) device based on a CdSe-Co3O4@TiO2 nanoflower. The device demonstrated ultrasensitive detection of viruses including hepatitis E virus (HEV), HEV-like particles (HEV-LPs), and SARS-CoV-2 spike protein in complex lysate solution. The enhanced photocurrent response of the PEC device can be attributed to the CdSe quantum dot sensitization effect and strong visible light absorption, improving system stability and energy conversion efficiency. The device achieved considerable sensitivity, good specificity, and acceptable stability, indicating its potential for affordable and portable biosensing capabilities.
Article
Chemistry, Multidisciplinary
Cheng-Kuo Tsai, Ching-Hsuan Huang, Jao-Jia Horng, Hui Lin Ong, Ruey-An Doong
Summary: A novel copper metal-organic framework (CuxO/MOF) material has been developed with excellent photocatalytic degradation and adsorption properties for the commonly used antibiotic ciprofloxacin (CIP) in the environment.
Article
Biochemical Research Methods
Fahmida Nasrin, Indra Memdi Khoris, Ankan Dutta Chowdhury, Sjakurrizal El Muttaqein, Enoch Y. Park
Summary: To prevent viral diseases, the development of a disposable, rapid, and convenient biosensor with high sensitivity and reliability is highly desired. In this study, a practical impedimetric biosensor has been created on a disposable electrode for the detection of two mosquito-borne viruses. The biosensor fabrication involves the formation of a conductive film, physical binding of gold nanoparticles-polyaniline, and biofunctionalization. The disposable electrode has been designed to detect Chikungunya virus and Zika virus separately with a wide linear range and low detection limits.
BIOTECHNOLOGY JOURNAL
(2023)
Article
Chemistry, Analytical
Eric R. Halabaso, Pooja Somnath Dayma, Jonathan W. L. Salvacion, Ruey-an Doong
Summary: Copper tungstate (CuWO4) decorated 2-dimensional reduced graphene oxide (CWO/rGO) nanocomposite with large specific surface area and abundant mesopores was synthesized and used for capacitive deionization (CDI). The CuWO4 provides electroactive sites and the rGO enhances electric conductivity and offers mesoporous channels for electrosorption of ions. The CWO/rGO exhibits high specific capacitances, specific electrosorption capacity, charge efficiency, low energy consumption, and long-term stability, making it a potential material for brackish water desalination.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2023)
Review
Electrochemistry
Michelle S. Carbonell, Al Rey C. Villagracia, Hui Lin Ong, Ruey-An Doong
Summary: This study provides a bibliometric analysis and recent developments on using biochar as fuel in the DC-SOFC, after adopting thermochemical conversion and activation methods. The dominant researchers from different countries and their contributions to the development of DC-SOFC are reported. The study also discusses the physicochemical characteristics of biochar and its effects on the electrochemical performance of DC-SOFC when used as fuel. The data reveals that other biomasses can also be used as DC-SOFC fuel through pyrolysis.
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
(2023)
Article
Nanoscience & Nanotechnology
Taisei Motomura, Kenshin Takemura, Toshimi Nagase, Nobutomo Morita, Tatsuo Tabaru
Summary: By using a magnetic mirror-type magnetron cathode, the temperature of the substrate can be controlled below the environmental temperature of less than 40°C in magnetron sputtering, allowing film deposition on low heat-resistant substrates.
Article
Chemistry, Multidisciplinary
Tzu-Heng Wang, Min-Jen Chen, YenJung Sean Lai, Ruey-an Doong, Paul Westerhoff, Bruce Rittmann
Summary: By integrating visible-light-emitting diodes, optical fibers, and O2-delivering hollow-fiber membranes, we have achieved low-energy and chemical-free photocatalytic production of hydrogen peroxide. This is made possible by utilizing a stable iron-based metal-organic framework photocatalyst fixed onto the optical fibers, improving light utilization and enabling catalyst reusability and O2 supply. The production rate of hydrogen peroxide in pure water is as high as 290 mM h-1 catalyst-g-1, which is 60 times higher than the best-reported values using photocatalytic slurries. The system also has a low energy cost of 2.3 kWh kgH2OH2O2-1 and can sustain high production rates for at least five repeated cycles (2 hours per cycle).
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2023)
Article
Biochemistry & Molecular Biology
Marina Ikegaya, Enoch Y. Park, Takatsugu Miyazaki
Summary: Glycoside hydrolase family 31 (GH31) contains a-glycoside hydrolases with different substrate specificities involved in various physiological functions. This study characterized two a-galactosidases, BsGH31_19 and FpGH31_19, and found that they showed high substrate specificity against a-(1?4)-linkages in a-(1?4)-galactobiose and globotriose, unlike other reported GH31 a-galactosidases PsGal31A and MYORG. Structural analyses revealed differences in amino acid residues and dimer interfaces between BsGH31_19 and FpGH31_19 compared to PsGal31A and MYORG, providing insights into the molecular diversity and evolutionary relationships of GH31 a-galactosidases and other a-galactosidase families.
Article
Biochemistry & Molecular Biology
Jaewook Lee, Ji-Heon Lee, Seung-Yeul Lee, Sin A. Park, Jae Hoon Kim, Dajeong Hwang, Kyung A. Kim, Han Sang Kim
Summary: In this study, biocompatible nanomaterials were synthesized using gallic acid (GA) and coated onto iron oxide nanoparticles (IONPs). The GA-IONPs exhibited excellent biocompatibility and antioxidant activity, effectively attenuating exosome secretion induced by H2O2-induced oxidative stress.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Immunology
Doddy Irawan Setyo Utomo, Hamizah Suhaimi, Nor Azila Muhammad Azami, Fazren Azmi, Mohd Cairul Iqbal Mohd Amin, Jian Xu
Summary: Efforts have been made to develop attenuated and subunit vaccines against SARS-CoV-2 using diverse virus-like particles (VLPs) as carriers for antigen display. Genetic or chemical methods can incorporate antigens or antigenic domains from SARS-CoV-2 into VLPs, allowing the screening of safer and more effective vaccine candidates. This review provides insights into the future development of SARS-CoV-2 VLP vaccines by summarizing the latest updates and perspectives on antigen display platforms.
Article
Nanoscience & Nanotechnology
Kenshin Takemura, Taisei Motomura, Wataru Iwasaki, Nobutomo Morita
Summary: The fabrication of nanoscale metal interface structures has been widely used in various fields due to their conducive electrical and optical properties. In this study, highly active gold electrodes with a specific surface topography were directly fabricated from raw rose petals. The use of these electrodes significantly improved the sensitivity in detecting arsenic(III) compared to smooth gold films. This research demonstrates the potential of using biomaterial interface structures to fabricate complex metal surfaces for highly sensitive detection.
ACS APPLIED NANO MATERIALS
(2023)
Review
Chemistry, Analytical
Sjaikhurrizal El Muttaqien, Indra Memdi Khoris, Sabar Pambudi, Enoch Y. Y. Park
Summary: In the past few decades, nanomaterials have been explored in the sensing research field. However, less attention has been given to their application in biosensors for pathogenic virus detection. This review focuses on 3D nanospheres, a type of nanostructured material, and their extensive use in various sensing techniques. Signal amplification strategies using different types of nanospheres are discussed, along with challenges related to toxicity and commercialization in the development of nanosphere-based biosensors.
Article
Engineering, Chemical
Thi Kim Anh Nguyen, Nguyen Thi Ngoc Anh, Manh Dung Nguyen, Van Thanh Nguyen, Ruey-an Doong
Summary: MXene material Ti3C2Tx was synthesized using a viable approach for enhanced capacitive deionization (CDI). The synthesized nanosheets showed hydrophilic and well-delaminated characteristics with large surface area and exceptional electrochemical performance. The material exhibited good cyclability and remarkable deionization capability during the CDI process.
SEPARATION AND PURIFICATION TECHNOLOGY
(2023)