Article
Chemistry, Analytical
Wenjing Wang, Yun Li, Axiu Nie, Gao-Chao Fan, Heyou Han
Summary: A lateral flow microRNA-21 biosensing platform was developed using a portable SERS reader and a catalytic hairpin assembly signal amplification strategy, achieving sensitive quantification of microRNA-21 with a detection limit as low as 84 fM. This approach shows great potential for amplified point-of-care biosensing for clinical diagnosis by detecting targets in serum samples.
Article
Chemistry, Analytical
Linyan Li, Xiaojun Fang, Jingqing Le, Yanhui Zheng, Xiarong Tan, Zhou Jiang, Hongxia Li, Jianguo Xu, Huo Xu
Summary: In this study, a cascade catalytic hairpin assembly (CCHA) method was developed for sensitive and selective detection of miRNA. The method showed high assay sensitivity, excellent sequence specificity, and the ability for intracellular imaging, demonstrating promising potential for biosensing, clinical diagnosis, or prognosis.
Article
Chemistry, Analytical
Li Zou, Xinghui Li, Yufen Lai
Summary: An enzyme-free colorimetric aptasensor was developed for the sensitive detection of kanamycin in complex samples, exhibiting high sensitivity and selectivity with a broad linear range and a limit of detection of 10 pM. The aptasensor integrates target-triggered catalytic hairpin assembly (CHA) amplification with the aggregation of DNA-gold nanoparticle probes, leading to a colorimetric readout that can be visualized by naked eye or measured by UV-vis spectroscopy. The aptasensor also demonstrates excellent applicability for kanamycin detection in complex samples with satisfactory recoveries and results comparable to those of the ELISA method, suggesting great potential applications in various fields.
MICROCHEMICAL JOURNAL
(2021)
Article
Chemistry, Analytical
Furui Jin, Danke Xu
Summary: The mi-CHA method detects miRNAs by changing the conformation of hairpin structured DNA probes on the chip surface, showing high sensitivity and specificity for clinical diagnosis.
ANALYTICA CHIMICA ACTA
(2021)
Article
Biophysics
Xia Li, Fang Yang, Chunfang Gan, Ruo Yuan, Yun Xiang
Summary: The researchers have developed a novel DNA hairpin assembly method by introducing palindrome sequences, which can image miRNA-155 in living tumor cells with high sensitivity, providing a new approach for early disease diagnosis and biological studies.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Analytical
Yan Wu, Cuicui Fu, Wenbing Shi, Jinyang Chen
Summary: Abnormal expression of miRNAs is closely associated with health disorders, making them potential biomarkers for early clinical diagnosis. The enzyme-free CHA signal amplification method shows significant potential in improving the sensitivity of miRNA detection strategies, with various sensing strategies based on this approach being developed.
Article
Chemistry, Analytical
Huo Xu, Danlong Chen, Lee Jia
Summary: A highly sensitive and specific amplification strategy for the detection of miRNA-155 based on a combination of nicked rolling circle amplification and catalyzed hairpin assembly has been developed. This method allows for early cancer diagnosis with amplified fluorescence signal and excellent specificity, showing potential for clinical use.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Analytical
Ya-Xin Wang, Dong-Xia Wang, Jing Wang, Bo Liu, An-Na Tang, De-Ming Kong
Summary: The study developed a highly integrated DNA nanoamplifier by anchoring hairpin probes on the surface of DNA nanolantern, enabling high sensitivity detection of miRNAs. The nanoamplifier showed enhanced signal amplification capability, accelerated reaction kinetics, and enabled miRNA imaging and analysis in living cells and mice.
Article
Chemistry, Analytical
Liuting Mo, Mingxiu Mo, Danlian Liang, Chan Yang, Weiying Lin
Summary: Improving the accuracy, sensitivity, and speed of intracellular miRNA imaging is crucial for early cancer diagnosis. This study proposes a strategy for imaging two distinct miRNAs using DNA tetrahedron-based catalytic hairpin assembly (DCHA). Two nanoprobes, DTH-13 and DTH-24, were prepared and their structures were functionalized DNA tetrahedrons with CHA hairpins responsive to miR-21 and miR-155. These DNA nanoparticles were used as carriers to easily enter living cells. The presence of miR-21 or miR-155 triggered CHA between DTH-13 and DTH-24, resulting in independent fluorescence signals. The sensitivity and kinetics of this system were significantly enhanced due to the DCHA strategy. The performance of this method was thoroughly investigated in various samples, and the results confirmed the potential of DTH nanoprobes as a diagnostic tool for early-stage cancer.
Article
Chemistry, Analytical
Terefe Tafese Bezuneh, Tadesse Haile Fereja, Shimeles Addisu Kitte, Haijuan Li, Yongdong Jin
Summary: An enzyme-free target-triggered catalytic hairpin assembly (CHA) was proposed for signal amplified fluorescence thrombin detection, demonstrating a limit of detection (LOD) of 165 pM and linear range from 0.2 to 100 nM. The method could be potentially applied in complex biological matrices for sensitive detection of thrombin.
MICROCHEMICAL JOURNAL
(2021)
Article
Biophysics
Pinru Chen, Luying Wang, Peipei Qin, Bin-Cheng Yin, Bang-Ce Ye
Summary: In this study, a one-step detection method for microRNAs based on the RNA-based catalytic hairpin assembly (CHA) circuit coupled with CRISPR-Cas12a was proposed. The target miRNA can specifically trigger RNA-based CHA and induce a configurational change of the blocked crRNAs into precursor crRNAs (pre-crRNAs), which can be processed into mature crRNAs to function by leveraging the inherent RNase activities of Cas12a. The developed circuit achieves a femtomolar detection limit and shows accurate detection of miRNA levels in different cell lines.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Analytical
Xiangyu Ma, Yuheng Ma, Onome Ejeromedoghene, Martha Kandawa-Schulz, Wei Song, Yihong Wang
Summary: The ultra-sensitive photochemical biosensor developed in this study is based on the target miRNA-triggered catalytic hairpin assembly (CHA) reaction. By linking Au nanoparticles/C3N4 nanosheets and CdS quantum dots through a double helix structure, a strong photocurrent is generated after the CHA reaction is triggered by the target miRNA. This design effectively eliminates nonspecific adsorption interference and false-positive signals, achieving a low detection limit of 92 aM. The biosensor demonstrates good reproducibility and selectivity in serum sample analysis, showing great potential in disease diagnostics and bioanalysis.
Article
Chemistry, Analytical
Qiang Liu, Mei Liu, Yan Jin, Baoxin Li
Summary: In this study, an enzyme-free signal amplification method for miRNA detection was developed using target-triggered catalytic hairpin assembly (CHA). The method showed high sensitivity and fast detection speed, with good recoveries in serum samples.
Article
Chemistry, Analytical
Junduan Dai, Chao Xing, Yuhong Lin, Yuqing Huang, Yuling Yang, Zhaowei Chen, Chunhua Lu, Huanghao Yang
Summary: A localized catalytic hairpin assembly reaction on DNA Origami was proposed for detection and imaging of intracellular microRNA-21, which offered faster reaction kinetics and increased sensitivity compared to traditional CHA reaction. DNA origami rectangle also served as a carrier to protect DNA hairpins from nuclease attack and could be easily integrated with reported DDSA-based strategy for wide applications.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Biophysics
Ye Zhang, Xiaohe Zhang, Bo Situ, Yuan Wu, Shihua Luo, Lei Zheng, Yurong Qiu
Summary: The study introduced a robust electrochemical biosensor using multifunctional DNA tetrahedrons for exo-miRNA analysis, demonstrating high sensitivity and rapid detection. The platform showed efficient quantification of exo-miRNA and profiling of tumor-associated miRNAs in breast cancer, with high sensitivity for breast tumor diagnosis and early detection.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Analytical
Yan Huang, Yiyi Zhang, Wenbin Hao, Huiting Lu, Haifeng Dong, Xueji Zhang
Summary: To achieve fast, convenient, and highly sensitive detection of microRNA (miRNA), palladium-gold (Pd-Au) bimetallic nanoplates with high photothermal effect were synthesized and combined with locked nucleic acid (LNA) detection probe to develop a photothermal lateral flow locked nucleic acid biosensor (P-LFLNAB) for miRNA detection. The P-LFLNAB enables sensitive and selective miRNA-21 detection with detection limit down to 0.094 pM using a smartphone with external thermal imager, which is 639.5 times lower than that of the conventional gold nanoparticles based colorimetric lateral flow biosensor and the most sensitive enzyme-free lateral flow biosensor for miRNA detection. Besides, the P-LFLNAB achieved single-base mismatch discrimination and can quantify signals in various cell lysates. This highly sensitive, selective and portable P-LFLNAB with smartphone readout has great potential in point-of-care test of miRNAs-related biomedical application.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Multidisciplinary
Xiangdan Meng, Sirong Sun, Chenchen Gong, Junyan Yang, Zhou Yang, Xueji Zhang, Haifeng Dong
Summary: In this study, we report the design of a Ti-based metal-organic framework (MIL) and silver nanoparticles (Ag NPs) heterostructure, MIL@Ag. It is found that the introduction of Ag NPs significantly improves the electron transfer efficiency and O2 adsorption capacity of MIL, leading to enhanced reactive oxygen species (ROS) generation for sonodynamic therapy (SDT). Under ultrasound irradiation, the activated electrons and holes from MIL and Ag NPs produce superoxide radicals and hydroxyl radicals, respectively, improving the therapeutic efficiency of SDT. MIL@Ag-PEG-mediated SDT demonstrates effective cancer cell killing and bacterial infection eradication. Thus, MIL@Ag-PEG provides a promising strategy for enhancing SDT performance through rational heterostructure design of sonosensitizers.
Article
Chemistry, Multidisciplinary
Chuan Fan, Yong Luo, Meng Tian, Mengyun Zhou, Lirong Wang, Tailin Xu, Xueji Zhang
Summary: Green synthesis is widely used in drug synthesis and nanomaterial preparation. The integration of automated micro-synthesis and high-throughput screening systems is an effective way to achieve green manufacturing. In this study, a one-click green synthesis platform is designed for the high-throughput screening of MNPs@Cu-MOF, which integrates droplet arrays-based micro-synthesis and Raman enhancement performance evaluation. The results show that the AgNPs@Cu-MOF exhibited the best Raman enhancement performance, indicating its potential applications in various fields.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Chemistry, Analytical
Shuangshuang Yang, Jinya Du, Menglian Wei, Yan Huang, Yufan Zhang, Yeyu Wang, Jinze Li, Wei Wei, Yuchun Qiao, Haifeng Dong, Xueji Zhang
Summary: Rapid, simple, sensitive and reliable approaches for quantifying biogenic amines in food samples are essential for food safety. Here, the authors reported a Fe3O4@Au nanoparticles based multimodal readout LFIA for rapid quantification of putrescine and histamine. The assay showed high sensitivity and could be easily expanded to detect other biogenic amines.
ANALYTICA CHIMICA ACTA
(2023)
Article
Biophysics
Mengyun Zhou, Yong Luo, Lirong Wang, Chuan Fan, Tailin Xu, Xueji Zhang
Summary: The outbreak of COVID-19 has posed a great challenge to global health systems. A rapid and sensitive POCT diagnostic platform for SARS-CoV-2 that can be deployed on-site is crucial in containing the epidemic. A portable microdroplet detection platform with temperature control and micro-stirring has been developed, enabling high-throughput and ultrafast COVID-19 diagnosis. The platform, utilizing a PN junction as the temperature controller and incorporating an ultrasonic micro-stirring unit, shows good linearity and low LOD, providing results in a short time. This integrated platform offers a promising tool for point-of-care diagnosis of COVID-19 in resource-limited settings.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Materials Science, Multidisciplinary
Chun-Yu Cheng, Yuan-Yuan Guo, Yi-Ming Zou, Amanda Jiamin Ong, Alfred Iing Yoong Tok, Shuzhou Li
Summary: The melting mechanisms of Pt-based multimetallic nanoparticles (NPs) were investigated using molecular dynamics (MD) simulation. The study found that the melting and coalescence behaviors of heterogeneous Pd-Pt NPs with different sizes and compositions varied, and PtPd alloy could form at the Pd/Pt interface before complete melting of Pd NP. Furthermore, a two-step melting process was observed in Pd/Pt/Ir trimetallic NPs.
Article
Nanoscience & Nanotechnology
Bo Niu, Delong Cai, Zhihua Yang, Xiaoming Duan, Wenjiu Duan, Donghui Long, Shuzhou Li, Dechang Jia, Yu Zhou
Summary: The high-temperature dielectric loss of hexagonal boron nitride (h-BN) ceramics can be reduced by orienting grains, leading to a minimized defect ions transport and lower dielectric loss.
SCRIPTA MATERIALIA
(2023)
Article
Chemistry, Analytical
Yong Luo, Mengyun Zhou, Chuan Fan, Yongchao Song, Lirong Wang, Tailin Xu, Xueji Zhang
Summary: This paper reports an integrated active enrichment platform for direct hand-held detection of COVID-19 nucleic acid in nanoliter samples without PCR. The platform comprises a capillary-assisted liquid-carrying system for sampling, an integrated circuit system for ultrasound output, and a cell-phone-based surface-enhanced Raman scattering (SERS) system. With the use of functionalized gold nanorods, this platform can actively assemble gold nanorods and achieve highly sensitive SERS detection of COVID-19 biomarkers in nanoliter samples within 5 minutes. The platform demonstrates high stability, repeatability, and selectivity, and shows potential for the detection of throat swab samples, making it a promising diagnostic tool for direct and rapid COVID-19 detection.
ANALYTICAL CHEMISTRY
(2023)
Article
Engineering, Environmental
Yi Xing, Mengyun Zhou, Xiangmei Liu, Minghang Qiao, Liping Zhou, Tailin Xu, Xueji Zhang, Xin Du
Summary: Chemodynamic therapy (CDT), which catalyzes the production of highly toxic reactive oxygen species (ROS), has been widely used in tumor therapy. However, the effectiveness of CDT is often limited by the poor tumor penetration of current nanomaterials. In this study, bioinspired jellyfish-like carbon/manganese nanomotors (JCMNs) were designed to achieve dual-propulsion for enhanced CDT therapy. The JCMNs utilized hydrogen peroxide (H2O2) and NIR light to propel themselves and improve tumor penetration and ROS generation, leading to improved CDT efficiency.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Multidisciplinary Sciences
Yuanmiao Sun, Jiarui Wang, Shibo Xi, Jingjing Shen, Songzhu Luo, Jingjie Ge, Shengnan Sun, Yubo Chen, John V. Hanna, Shuzhou Li, Xin Wang, Zhichuan J. Xu
Summary: In this study, the relationship between surface reconstruction of spinel electrocatalysts and metal-oxygen covalency polarity was investigated. It was found that a stronger metal-oxygen covalency contributes to a more thorough surface reconstruction towards oxyhydroxides. The researchers also developed a criterion to evaluate the reconstruction degree and provide guidelines for cation selection in spinel pre-catalyst design.
NATURE COMMUNICATIONS
(2023)
Article
Materials Science, Ceramics
Yuchen Liu, Yu Zhou, Dechang Jia, Zhihua Yang, Wenjiu Duan, Daxin Li, Shuzhou Li, Ralf Riedel, Bin Liu
Summary: Using ab-initio calculations, the atomic structure and mechanical properties of amorphous silicoboron carbonitride ceramics with 13 different compositions in the Si-BN-C phase diagram were investigated. The chemical bonds and local structures in the amorphous network are dependent on the elemental composition. The mechanical properties of SiBCN ceramics are greatly influenced by their local structural characteristics. SiB2C3N2 and SiB3C2N3 with low Si contents and moderate C and/or BN contents exhibit high elastic moduli, high tensile/shear strengths, and good debonding capability. The optimization of SiBCN ceramics can be guided based on these discoveries.
JOURNAL OF ADVANCED CERAMICS
(2023)
Review
Chemistry, Multidisciplinary
Conghui Liu, Jingyu Chen, Jiahui Liang, Tailin Xu, Xueji Zhang
Summary: Artificial micro/nanomotors have shown promising potential in nucleic acid biosensing, enabling targeted delivery of detection probes and improving sensitivity and specificity. This comprehensive overview discusses the advancements in nucleic acid biosensing through the integration of micro/nanomotors over the past decade. Various applications of micro/nanomotors in nucleic acid biosensing, including fluorescence recovery-based biosensing and aggregation-enhanced biosensing, are explored. Challenges hindering their practical applications and future development prospects are also discussed.
Article
Chemistry, Multidisciplinary
Yiding Ma, Yilin Cao, Tao Yu, Zhixiang Zhang, Weipeng Lai, Chao Chen, Linyuan Wen, Yingzhe Liu
Summary: This study successfully predicted the polymorphs of CHON-based energetic molecules with high accuracy and good efficiency using a tailor-made polarizable force field (TMPFF) and the USPEX evolutionary algorithm.
Article
Chemistry, Analytical
Jingyu Xiao, Shuxin Zhang, Qingzhou Liu, Tailin Xu, Xueji Zhang
Summary: This study presents a microfluidic-based wearable plasmonic microneedle sensor for monitoring uric acid. The sensor shows excellent reproducibility and sensitivity, enabling fast identification of target molecules, which may contribute to efficient medical healthcare and early disease prevention.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
