Article
Chemistry, Analytical
Qiang Liu, Mei Liu, Yan Jin, Baoxin Li
Summary: In this study, an enzyme-free signal amplification method for miRNA detection was developed using target-triggered catalytic hairpin assembly (CHA). The method showed high sensitivity and fast detection speed, with good recoveries in serum samples.
Article
Chemistry, Analytical
Xiaojia Zhao, Zicheng Zhu, Rong Zou, Lingyun Wang, Hang Gong, Changqun Cai
Summary: A 3D DNA walker was developed for the reliable and quantitative detection of H5N1 DNA, demonstrating excellent analytical sensitivity and specificity with a detection limit of 60 pM. The method showed potential for clinical applications by selectively detecting H5N1 DNA in clinical blood serum samples.
MICROCHEMICAL JOURNAL
(2021)
Article
Biophysics
Pinru Chen, Luying Wang, Peipei Qin, Bin-Cheng Yin, Bang-Ce Ye
Summary: In this study, a one-step detection method for microRNAs based on the RNA-based catalytic hairpin assembly (CHA) circuit coupled with CRISPR-Cas12a was proposed. The target miRNA can specifically trigger RNA-based CHA and induce a configurational change of the blocked crRNAs into precursor crRNAs (pre-crRNAs), which can be processed into mature crRNAs to function by leveraging the inherent RNase activities of Cas12a. The developed circuit achieves a femtomolar detection limit and shows accurate detection of miRNA levels in different cell lines.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Analytical
Jianxin Tang, Tao Wang, Qing Li
Summary: A novel enzyme-free and label-free fluorescence assay based on DNA silver nanocluster-lightened catalytic hairpin assembly has been developed for sensitive and cost-effective detection of mRNA expression level. The strategy relies on the use of two hairpin probes with DNA-stabilized silver nanoclusters to specifically target mRNA, showing a linear relationship between fluorescence intensity and concentration of surviving mRNA with a detection limit of about 45 pM. This platform has great potential for detection of disease-related biomarkers and early clinical theragnostic.
MICROCHEMICAL JOURNAL
(2021)
Article
Chemistry, Analytical
Ya-Xin Wang, Dong-Xia Wang, Jing Wang, Bo Liu, An-Na Tang, De-Ming Kong
Summary: The study developed a highly integrated DNA nanoamplifier by anchoring hairpin probes on the surface of DNA nanolantern, enabling high sensitivity detection of miRNAs. The nanoamplifier showed enhanced signal amplification capability, accelerated reaction kinetics, and enabled miRNA imaging and analysis in living cells and mice.
Article
Chemistry, Analytical
Terefe Tafese Bezuneh, Tadesse Haile Fereja, Shimeles Addisu Kitte, Haijuan Li, Yongdong Jin
Summary: An enzyme-free target-triggered catalytic hairpin assembly (CHA) was proposed for signal amplified fluorescence thrombin detection, demonstrating a limit of detection (LOD) of 165 pM and linear range from 0.2 to 100 nM. The method could be potentially applied in complex biological matrices for sensitive detection of thrombin.
MICROCHEMICAL JOURNAL
(2021)
Article
Chemistry, Analytical
Junduan Dai, Chao Xing, Yuhong Lin, Yuqing Huang, Yuling Yang, Zhaowei Chen, Chunhua Lu, Huanghao Yang
Summary: A localized catalytic hairpin assembly reaction on DNA Origami was proposed for detection and imaging of intracellular microRNA-21, which offered faster reaction kinetics and increased sensitivity compared to traditional CHA reaction. DNA origami rectangle also served as a carrier to protect DNA hairpins from nuclease attack and could be easily integrated with reported DDSA-based strategy for wide applications.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Biophysics
Ye Zhang, Xiaohe Zhang, Bo Situ, Yuan Wu, Shihua Luo, Lei Zheng, Yurong Qiu
Summary: The study introduced a robust electrochemical biosensor using multifunctional DNA tetrahedrons for exo-miRNA analysis, demonstrating high sensitivity and rapid detection. The platform showed efficient quantification of exo-miRNA and profiling of tumor-associated miRNAs in breast cancer, with high sensitivity for breast tumor diagnosis and early detection.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Analytical
Jiawei Shen, Tingting Li, Min Wang, Bo Yao
Summary: This study proposes an isothermal and enzyme-free miRNA assay based on CHA amplification and ICP-MS detection. By utilizing hybridization reaction and CHA cycles, high sensitivity and specificity analysis of miRNA-141 were achieved. The method also demonstrated multiple detecting capability and the potential of becoming a high-throughput method.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
Wen Yun, Yiyan Lin, Ruiqi Wang, Xia Ha, Nana Xie, Xiaoli Xiong, Zhengwei Xiong, Ning Li, Xingmin Wang, Lizhu Yang
Summary: An efficient and innovative strategy for colorimetric detection of bisphenol A (BPA) is presented, based on target-induced catalytic hairpin assembly (CHA) and DNAzyme-caused fragment self-assembly hybridization chain reaction (HCR). The biosensor has high sensitivity for BPA detection at concentrations ranging from 0.8 pM to 500 pM, with a detection limit as low as 0.2 pM, providing a promising prospective ultrasensitive detection of BPA.
ANALYTICAL METHODS
(2023)
Article
Nanoscience & Nanotechnology
Chun Hong Li, Wen Yi Lv, Fei Fan Yang, Shu Jun Zhen, Cheng Zhi Huang
Summary: Simultaneous detection and imaging of two miRNAs was achieved using a DTN probe, showing great potential for improving detection accuracy.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Biophysics
Xia Li, Fang Yang, Chunfang Gan, Ruo Yuan, Yun Xiang
Summary: The researchers have developed a novel DNA hairpin assembly method by introducing palindrome sequences, which can image miRNA-155 in living tumor cells with high sensitivity, providing a new approach for early disease diagnosis and biological studies.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Analytical
Yan Wu, Cuicui Fu, Wenbing Shi, Jinyang Chen
Summary: Abnormal expression of miRNAs is closely associated with health disorders, making them potential biomarkers for early clinical diagnosis. The enzyme-free CHA signal amplification method shows significant potential in improving the sensitivity of miRNA detection strategies, with various sensing strategies based on this approach being developed.
Article
Chemistry, Multidisciplinary
Yuqiu He, Qing Wang, Chen Hong, Ruomeng Li, Jinhua Shang, Shanshan Yu, Xiaoqing Liu, Fuan Wang
Summary: Synthetic catalytic DNA circuits have potential for sensitive intracellular imaging, but their selectivity and efficiency are limited by off-site signal leakage and inefficient on-site activation. An endogenously activated DNAzyme strategy was integrated with a catalytic DNA circuit for selective and efficient microRNA imaging in vivo. The on-site modulation strategy allows these circuits to be expanded in biological systems.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Multidisciplinary
Huimin Wang, Yuqiu He, Jie Wei, Hong Wang, Kang Ma, Yangjie Zhou, Xiaoqing Liu, Xiang Zhou, Fuan Wang
Summary: This study developed a versatile autocatalytic DNA circuit for high-performance in vivo bioanalysis. The circuit showed sensitive and selective detection of analytes, and enabled robust miRNA imaging in living cells and mice. These findings expand the toolbox for accurate diagnosis and programmable therapeutics.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Chemistry, Analytical
Yujia Ying, Huilin Li
Summary: Enzymes play a crucial role as biological catalysts in accelerating biochemical reactions in living organisms. This study proposes a convenient method for monitoring enzymatic catalytic processes using native top-down mass spectrometry. By exploring the heterogeneity of the chymotrypsin sample and using tandem mass spectrometry, the researchers were able to monitor covalent and noncovalent enzymatic complexes, substrates, and products during catalysis. The results demonstrate that this method has the potential to be a promising tool for characterizing biocatalysts.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Kang Yang, Shuaibo Shi, Jinyu Wu, Shaolong Han, Shengdi Tai, Shishen Zhang, Kun Zhang
Summary: H2O and D2O are important analogues closely related to various industries and monitoring fields. This study successfully distinguishes and detects H2O and D2O by designing a novel eu(III)-macrocycle with dual emitters, Eu-2a, and conducting fluorescence titration experiments.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Julia Kuligowski, Alvaro Perez-Rubio, Marta Moreno-Torres, Polina Soluyanova, Judith Perez-Rojas, Ivan Rienda, David Perez-Guaita, Eugenia Pareja, Ramon Trullenque-Juan, Jose Castell, Marcha Verheijen, Florian Caiment, Ramiro Jover, Guillermo Quintas
Summary: This study introduces a novel variable selection approach called cluster PLS (c-PLS) to assess the joint impact of variable groups selected based on biological characteristics on the predictive performance of a multivariate model. The usefulness of c-PLS is shown using miRNomic and metabolomic datasets obtained from the analysis of liver tissue biopsies.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Meixue Lai, Lijie Zhong, Siyi Liu, Yitian Tang, Tingting Han, Huali Deng, Yu Bao, Yingming Ma, Wei Wang, Li Niu, Shiyu Gan
Summary: This study presents a method for constructing wearable sweat electrolyte sensors using carbon fiber-based solid-contact ion-selective electrodes (SC-ISEs). By using carbon fibers extracted from commercial cloth as electrode material, the cost and reproducibility issues of flexible SC-ISEs were addressed. The results showed that the carbon fiber-based SC-ISEs exhibited reversible voltammetric and stable impedance performances, and had high reproducibility of standard potentials between normal and bending states.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Yang, Ying Liu, Min Deng, Peipei Wang, Dan Cheng, Songjiao Li, Longwei He
Summary: A near-infrared fluorescent probe has been developed to accurately measure the levels of peroxynitrite (ONOO-) in the endoplasmic reticulum (ER) in acute lung injury (ALI). The probe demonstrated rapid response, high selectivity, good sensitivity, and enhanced fluorescence intensity in response to ONOO-. It was successfully used to detect changes in ONOO- levels and showed significant increases in an ALI cell model and an ALI mouse model.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yanping Wang, Yuemei Chen, Kejun Li, Jinrong Zhou, Xin Yuan, Mei Zhang, Ke Huang
Summary: In this work, a portable analytical system based on point discharge chemical vapor generation atomic emission spectrometry (PD-CVG-AES) coupling with gold filament enrichment was designed. The highly sensitive analysis of Hg2+ indirectly realized the detection of ascorbic acid (AA). The measurement is based on the fact that Ag+ can decrease the concentration of Hg2+ by forming Ag-Hg amalgam in the presence of the reductant SnCl2, while AA can pre-reduce Ag+ to Ag0, leading to the generation of silver nanoparticles (Ag NPs). The developed novel analytical strategy broadens the application of microplasma-based AES and offers a higher level of sensitivity compared to current AA detection techniques.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yundi Huang, Bo Song, Kaiwen Chen, Deshu Kong, Jingli Yuan
Summary: This study developed two lysosome-targetable background-free TGL probes for efficient and accurate detection of 1O2, which can be used for monitoring endogenous 1O2 concentrations in lysosomes and discriminating variability induced by different photosensitizers. Furthermore, a smart luminescent sensor film was successfully prepared for on-site 1O2 production detection during PDT processes, offering a promising clinical monitoring tool for skin diseases.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Wei Lang, Jia-Mei Qin, Qian-Yong Cao
Summary: A novel polymer-based probe P1 was successfully synthesized for fluorescently ratiometric sensing of H2S with high selectivity and sensitivity. A smartphone sensing platform was constructed to conduct visual quantitative detection of H2S. P1 can be employed in evaluating the level fluctuations of H2S in living cells, testing water samples/wine samples, and monitoring food freshness.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yuanyuan Qin, Shuda Liu, Shuyun Meng, Dong Liu, Tianyan You
Summary: The study developed a split aptamer-based sandwich-type ratiometric biosensor for the detection of 17 beta-estradiol (E2) using photoelectrochemical and electrochemical assays. The biosensor utilized split aptamer fragments to recognize E2 and trigger a hybridization chain reaction, resulting in the production of double-stranded DNA labeled with CdTe quantum dots. This DNA complex was able to sensitize CdTe quantum dots and generate response signals for E2 detection. The developed biosensor demonstrated high sensitivity and accuracy with two linear ranges and low detection limits.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Siriwan Teepoo, Jongjit Jantra, Khaunnapa Panapong, David Taiwo Ajayi
Summary: A novel immunochromatographic assay based on hyperbranched Au plasmonic blackbodies with a smartphone readout was developed for rapid and sensitive detection of leucomalachite green residues in fish and shrimp products.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Andrea L. Larraga-Urdaz, Borja Moreira-Alvarez, Jorge Ruiz Encinar, Jose M. Costa-Fernandez, Maria Luisa Fernandez-Sanchez
Summary: A major challenge in the 21st century is the development of point-of-care diagnostic tools. In this study, a highly sensitive and simple bioassay using AuNPs and MNAzymes was developed for rapid detection and quantification of miRNA-4739. The proposed strategy shows potential for breast cancer diagnosis.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Xiaohan Zhao, Anyu Wang, Lingzi Zhai, Jiuhe Gao, Sizhe Lyu, Yingshan Jiang, Tian Zhong, Ying Xiao, Xi Yu
Summary: A novel method using polystyrene-coated magnetic nanoparticles for extracting monohydroxy polycyclic aromatic hydrocarbons from urine samples was investigated. The proposed method is simple, sensitive, and efficient, with desirable sensitivity for analyzing low-abundance metabolites in large volumes of complex urine samples.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Quan, Yuqing Zeng, Wenke Zhang, Fengfeng Li, Mengjiao Li, Zhihe Qing, Linlin Wu
Summary: In this study, a visual and reusable biosensor was developed for detecting substrates that are closely associated with human physiological health. The immobilized oxidase showed higher stability and sensitivity under harsh conditions, enabling reliable detection of substrates in complex fluids.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Dongjuan Wang, Xiuqian Ding, Jinling Xie, Juan Wang, Guanhao Li, Xin Zhou
Summary: In this study, a three-in-one sensor was developed for real-time detection of biogenic amines (BAs) with high sensitivity and selectivity. The sensor showed multimodal responses and could be used to fabricate portable devices for on-site non-destructive assessment of food spoilage indicators.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Jinting Meng, Zihao Xu, Shasha Zheng, Hongqun Yang, Tianfu Wang, Hong Wang, Yingwei Zhang
Summary: This study demonstrates the development of a cascade signal amplification system using a multi-pedal DNA walker and strand displacement reactions for the electrochemical detection of miRNA-155. The biosensor exhibited high sensitivity, selectivity, and the potential for clinical applications.
ANALYTICA CHIMICA ACTA
(2024)