Article
Chemistry, Analytical
Dan Zhu, Yaqi Wei, Tao Sun, Chengwen Zhang, Lei Ang, Shao Su, Xiuhai Mao, Qian Li, Chunhai Fan, Xiaolei Zuo, Jie Chao, Lianhui Wang
Summary: This study developed a method using rationally designed one-pot assembled multicolor tetrahedral DNA frameworks (TDFs) encoded with multicomponent nucleic acid enzymes (MNAzymes) for signal-amplified multiple miRNAs imaging in living cells with high sensitivity and selectivity.
ANALYTICAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Shenglin Cai, Thomas Pataillot-Meakin, Akifumi Shibakawa, Ren Ren, Charlotte L. Bevan, Sylvain Ladame, Aleksandar P. Ivanov, Joshua B. Edel
Summary: The study presents a new sensing platform for miRNA detection, offering high sensitivity and specificity directly in human serum, and demonstrating monitoring of miR-141-3p and miR-375-3p in various stages of prostate cancer.
NATURE COMMUNICATIONS
(2021)
Article
Chemistry, Analytical
Hua Chai, Yuguo Tang, Peng Miao
Summary: Ultra-sensitive electrochemical analysis of miRNA is achieved through the construction of a three-dimensional DNA tetrahedral nanostructure, and successfully applied for the detection of miRNA in cell and serum samples.
ANALYTICAL CHEMISTRY
(2022)
Review
Chemistry, Multidisciplinary
Thomas Jet, Guillaume Gines, Yannick Rondelez, Valerie Taly
Summary: MicroRNA detection is a crucial challenge in analytical chemistry, with the need for high sensitivity, specificity, robustness, and quantitative analysis. The ability to perform multiplexed detection of miRNA concentrations is important for recognizing disease signatures and new innovative detection methods have been developed to address this challenge.
CHEMICAL SOCIETY REVIEWS
(2021)
Article
Chemistry, Multidisciplinary
Wen-Hsin Chang, Yi-Fang Lee, Yen-Wenn Liu, Itamar Willner, Wei-Ching Liao
Summary: A method for synthesizing DNA-based acrylamide hydrogel microcapsules loaded with quantum dots as readout signal is introduced, which can detect cancer-associated microRNAs. The detection sensitivity can be improved by coupling the microcapsules with strand displacement amplification.
Article
Nanoscience & Nanotechnology
Liangliang Hao, Renee T. Zhao, Nicole L. Welch, Edward Kah Wei Tan, Qian Zhong, Nour Saida Harzallah, Chayanon Ngambenjawong, Henry Ko, Heather E. Fleming, Pardis C. Sabeti, Sangeeta N. Bhatia
Summary: Protease profiling using DNA-barcoded nanosensors and CRISPR amplification enables portable cancer diagnosis. Synthetic biomarkers are bioengineered sensors that generate molecular reporters in diseased microenvironments. By using chemically stabilized nucleic acids and CRISPR nucleases, diagnostic signals can be produced in biofluids. The study shows that DNA-encoded nanosensors can non-invasively detect and differentiate disease states in tumor models, and the CRISPR-Cas amplification can be used for point-of-care paper diagnostic tools.
NATURE NANOTECHNOLOGY
(2023)
Article
Chemistry, Analytical
Shuai Wang, Liyan Zhang, Ailing Kan, Xiaowen Xu, Nan Zhang, Wei Jiang
Summary: In this study, a MnO2 nanosheet-mediated target-binding-induced fluorescence resonance energy transfer (FRET) strategy was developed for detection and imaging of multiplexed miRNAs in living cells. The strategy successfully enabled simultaneous imaging of miRNA-373 and miRNA-96 in MDA-MB-231 and L02 cells, revealing higher expression levels of target miRNAs in MDA-MB-231 cells compared to L02 cells. This approach provides an alternative method for simultaneous accurate detection and imaging of multiplexed miRNAs with potential biomedical applications.
Article
Materials Science, Biomaterials
Zhe Dong, Xizhu Xu, Jing Ni, Yuancheng Li, Kang An, Ling Meng, Han Wu
Summary: A novel aptamer-functionalized cruciate DNA probe has been developed for imaging multiple low-abundance miRNAs in living cells. It offers improved stability, efficient delivery, and amplified signals for sensitive miRNA detection.
JOURNAL OF MATERIALS CHEMISTRY B
(2022)
Article
Biophysics
Sara Cavallaro, Petra Haag, Siddharth S. Sahu, Lorenca Berisha, Vitaliy O. Kaminskyy, Simon Ekman, Rolf Lewensohn, Jan Linnros, Kristina Viktorsson, Apurba Dev
Summary: Liquid biopsies based on extracellular vesicles (EVs) show promise for tumor treatment monitoring, with a multiplexed electrokinetic sensor capable of profiling surface protein markers in small EVs (sEVs) from non-small-cell lung cancers (NSCLC). The method detects differences in streaming current generated by EV binding to functionalized microcapillaries, allowing for simultaneous detection of multiple surface markers. Analysis of EVs isolated from the pleural effusion fluids of NSCLC patients reveals potential for monitoring treatment responses and identifying distinct interpatient differences in marker expression levels.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Analytical
Xiaoshuang Zhao, Yi Xu, Xianqiang Mi
Summary: miRNA analysis is crucial for accurate cancer diagnosis, treatment, and prognosis, particularly in multiplexed miRNA imaging. This study developed a novel fluorescence emission intensity (FEI) encoding strategy based on a tetrahedron DNA framework (TDF) carrier and FRET effect between Cy3 and Cy5. By constructing six FEI-encoded TDF (FEI-TDF) samples with different labeling numbers of Cy3 and Cy5, distinct FEIs and colors were observed in vitro. The stability of FEIs was improved by dividing the ranges of FEIs, and five codes with good discrimination were developed. The excellent biocompatibility of the TDF carrier was confirmed before intracellular imaging. Barcode probes based on specific FEI-TDF samples enabled multiplexed imaging of miRNA-16, miRNA-21, and miRNA-10b, showing different fluorescence merged colors in MCF-7 cells. FEI-TDFs provide a new research perspective for future development of fluorescence multiplexing strategies.
ANALYTICAL METHODS
(2023)
Article
Chemistry, Analytical
Guangyuan Dong, Yunsong An, Peikai Yan, Jiahui Wu, Chao Li, Tao Liu
Summary: The zeta potential-based assay described in the study allows for amplified detection of telomerase activity with improved signal-to-noise ratio and sensitivity. The method is simple and time-saving, potentially serving as a technological innovation in the field of telomerase analysis.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Biophysics
Sifan Meng, Runzhi Chen, Jingxuan Xie, Jing Li, Jiayi Cheng, Yanan Xu, Haiting Cao, Xiaofeng Wu, Qiang Zhang, Houyu Wang
Summary: This study developed an innovative 3D surface-enhanced Raman scattering holography sensing strategy for rapid, sensitive, and multiplexed detection of human breast cancer-associated miRNAs. By spatially separating amplified miRNAs on silicon SERS substrates and using different Raman reporters as SERS tags, the detection of miRNAs was completed quickly and with high sensitivity.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Analytical
Xue Dong, Qingze Zeng, Li Dai, Xiang Ren, Wei Cao, Huangxian Ju, Qin Wei
Summary: In this study, a novel signal on-amplified-off detection mode for progesterone (P4) was proposed, utilizing hafnium oxide (HfO2) as an electrochemiluminescence (ECL) emitter and Ni-doped ZnO (Ni-ZnO) as a coreaction accelerator. The P4 analysis using this strategy significantly improved sensitivity and specificity, offering a practical method for biomolecule detection beyond the scope of this study.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Jing Zhang, Meifeng Hou, Guanyu Chen, Huifang Mao, Wenqian Chen, Wenshen Wang, Jinghua Chen
Summary: An electrochemical biosensor based on DNA nano-bridge was developed for detecting exosomal miR-21 with high sensitivity and selectivity, showing potential application in early diagnosis of cancer.
CHINESE CHEMICAL LETTERS
(2021)
Article
Chemistry, Analytical
Ruomeng Li, Fengzhe Li, Yanping Zhang, Yuqiu He, Yushi Wang, Fuan Wang
Summary: Accurate diagnosis requires the development of multiple guaranteed DNA circuits. We developed a compact-yet-efficient hierarchical DNA hybridization (HDH) circuit for simultaneous analysis of multiple miRNAs and specifically discriminating cancer cells. The HDH circuit enables programmable multiple microRNA activations and localized cascaded signal amplification. It provides a powerful toolkit for accurate diagnostics and pathological evolution.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Kai Shi, Baoting Dou, Jianmei Yang, Ruo Yuan, Yun Xiang
ANALYTICA CHIMICA ACTA
(2016)
Article
Chemistry, Analytical
Jianmei Yang, Baoting Dou, Ruo Yuan, Yun Xiang
ANALYTICAL CHEMISTRY
(2016)
Article
Biophysics
Baoting Dou, Jianmei Yang, Kai Shi, Ruo Yuan, Yun Xiang
BIOSENSORS & BIOELECTRONICS
(2016)
Article
Chemistry, Multidisciplinary
Cuiyun Yang, Baoting Dou, Jianmei Yang, Ruo Yuan, Yun Xiang
CHEMICAL COMMUNICATIONS
(2016)
Article
Chemistry, Analytical
Baoting Dou, Jin Li, Bingying Jiang, Ruo Yuan, Yun Xiang
ANALYTICA CHIMICA ACTA
(2018)
Article
Chemistry, Analytical
Baoting Dou, Jianmei Yang, Ruo Yuan, Yun Xiang
ANALYTICAL CHEMISTRY
(2018)
Article
Chemistry, Analytical
Baoting Dou, Jin Li, Bingying Jiang, Ruo Yuan, Yun Xiang
ANALYTICAL CHEMISTRY
(2019)
Article
Chemistry, Analytical
Xia Li, Baoting Dou, Ruo Yuan, Yun Xiang
SENSORS AND ACTUATORS B-CHEMICAL
(2019)
Article
Chemistry, Analytical
Baoting Dou, Lin Xu, Bingying Jiang, Ruo Yuan, Yun Xiang
ANALYTICAL CHEMISTRY
(2019)
Article
Biophysics
Jianling Chen, Ji Yan, Qiumei Feng, Xiangmin Miao, Baoting Dou, Po Wang
Summary: The study proposed a label-free and enzyme-free fluorescence strategy based on CDs-SH for highly sensitive detection of miRNA using strand displaced amplification (SDA), which achieved a low limit of detection.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Electrochemistry
Hui Zhou, Liming Zhao, Yajun Hong, Baoting Dou, Po Wang
Summary: A simple electrochemical sensing system based on DNA triple helix and toehold-mediated strand displacement reaction (TSDR) has been developed for the monitoring of let 7a in human serum, which achieves highly selective and sensitive detection of microRNAs. The system allows for discrimination of let 7 miRNAs family members and detection of circulating miRNAs in human serum, providing a promising approach for early clinical diagnostics.
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
(2021)
Article
Chemistry, Multidisciplinary
Hui Shen, Zhimin Li, Baoting Dou, Qiumei Feng, Po Wang
Summary: An amplified DNA logic sensor was developed for the identification of multiple biomarkers. It utilized a V-shaped probe structure and a strand displacement reaction to generate silver nanoclusters (AgNCs) for electrocatalytic reduction of H2O2. The sensor achieved sensitive detection of methylated DNA and microRNA 122, with detection limits of 3.4 and 4.1 fM, respectively. It can be used for clinical serum sample analysis and offers convenience, low cost, and versatility without electroactive label modification, making it valuable for disease diagnosis and research.
CHEMICAL COMMUNICATIONS
(2023)
Article
Chemistry, Multidisciplinary
Baoting Dou, Hui Zhou, Yajun Hong, Liming Zhao, Po Wang
Summary: A newly developed electrochemical sensing system allows for the selective detection of microRNA 122 with high sensitivity, low cost, and easy manipulation, which is significant for disease diagnosis and molecular biology research.
CHEMICAL COMMUNICATIONS
(2021)
Article
Chemistry, Analytical
Jianmei Yang, Baoting Dou, Ruo Yuan, Yun Xiang
ANALYTICAL CHEMISTRY
(2017)
Article
Biophysics
Kai Shi, Baoting Dou, Jianmei Yang, Ruo Yuan, Yun Xiang
BIOSENSORS & BIOELECTRONICS
(2017)