Article
Chemistry, Multidisciplinary
Meirong Cui, Dan Zhang, Qingfu Wang, Jie Chao
Summary: DNAzymes hold great promise as transducing agents for the analysis of intracellular biomarkers. However, their low delivery efficiency and limited signal amplification capability hinder their application in low-abundance biomarker analysis. In this study, a general strategy to design an intelligent, autocatalytic DNAzyme biocircuit is developed for amplified microRNA imaging in living cells. The DNAzyme biocircuit is constructed based on a nanodevice composed of catalytic hairpin assembly (CHA) and DNAzyme biocatalytic functional units, sustained by Au nanoparticles (AuNPs) and MnO2 nanosheets (CD/AM nanodevices). The intelligent autocatalytic multilayer DNAzyme biocircuit can effectively avoid signal leakage and obtain high amplification gain, expanding the application of programmable complex DNA nanocircuits in biosensing, nanomaterial assembly, and biomedicine.
Article
Chemistry, Analytical
Ruomeng Li, Fengzhe Li, Yanping Zhang, Yuqiu He, Yushi Wang, Fuan Wang
Summary: Accurate diagnosis requires the development of multiple guaranteed DNA circuits. We developed a compact-yet-efficient hierarchical DNA hybridization (HDH) circuit for simultaneous analysis of multiple miRNAs and specifically discriminating cancer cells. The HDH circuit enables programmable multiple microRNA activations and localized cascaded signal amplification. It provides a powerful toolkit for accurate diagnostics and pathological evolution.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Shanshan Yu, Jinhua Shang, Shizhen He, Qing Wang, Ruomeng Li, Yingying Chen, Xiaoqing Liu, Fuan Wang
Summary: In this study, a high-contrast in vivo imaging of microRNA (miRNA) was achieved by integrating an entropy-driven catalytic DNA amplification machine with on-site amplified substrate exposure procedure. The undesired signal leakage was eliminated by grafting substrate subunits into an auxiliary DNA polymerization amplification accessory. The intact machine-propelling functions were restored by cell-specific mRNA, ensuring the high specificity of on-site amplified miRNA imaging. This intelligent machinery shows great potential in biomedical diagnosis.
Article
Chemistry, Analytical
Chang Xue, Mengxue Luo, Lei Wang, Congcong Li, Shuyao Hu, Xin Yu, Pei Yuan, Zai-Sheng Wu
Summary: DNAzymes with enzymatic activity selected from random DNA pools have led to the development of an autonomous-motion molecular machine for sensitive imaging of different intracellular microRNAs. The machine utilizes a self-feedback circuit to achieve sustainable autonomous operation and sensitive detection of miRNA-21, showing great potential in molecular biology research and early diagnosis of human diseases.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Wen-Hsin Chang, Yi-Fang Lee, Yen-Wenn Liu, Itamar Willner, Wei-Ching Liao
Summary: A method for synthesizing DNA-based acrylamide hydrogel microcapsules loaded with quantum dots as readout signal is introduced, which can detect cancer-associated microRNAs. The detection sensitivity can be improved by coupling the microcapsules with strand displacement amplification.
Article
Chemistry, Multidisciplinary
Yanan Peng, Zhijun Gao, Bin Qiao, Dongxia Li, Huajie Pang, Xiangde Lai, Qiumei Pu, Rui Zhang, Xuan Zhao, Guangyuan Zhao, Dan Xu, Yuanyuan Wang, Yuxiang Ji, Hua Pei, Qiang Wu
Summary: In this study, size-controlled 9-tile nanoarrays were designed to achieve caveolae-mediated endocytosis and amplify the imaging of microRNAs (miRNAs). These nanoarrays showed high sensitivity and specificity for miRNAs, bypassing lysosomal traps and delivering miRNAs efficiently into the cytoplasm.
Article
Chemistry, Multidisciplinary
Yong-Xiang Wu, Dailiang Zhang, Xiaoxiao Hu, Ruizi Peng, Junbin Li, Xiaobing Zhang, Weihong Tan
Summary: Novel theranostic nanosystems based on two-photon fluorescence have been designed and prepared for the diagnosis and therapy of various cancers. These nanosystems can be used for intracellular imaging and drug delivery in cancer cells, enhancing imaging depth and tissue spatial localization.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Optics
Yusong Sun, Huang Jian, Dongfeng Shi, Linbin Zha, Zijun Guo, Kee Yuan, Shunxing Hu, Yingjian Wang
Summary: This study proposes an approach of cosinusoidal encoding multiplexed structured illumination multispectral ghost imaging, which can capture the multispectral image of the target object within a short period of time while maintaining high imaging efficiency. The core of the approach is the novel encoding strategy that enables easy decoding and reconstruction of multispectral images via Fourier transform.
Article
Materials Science, Biomaterials
Zhe Dong, Xizhu Xu, Jing Ni, Yuancheng Li, Kang An, Ling Meng, Han Wu
Summary: A novel aptamer-functionalized cruciate DNA probe has been developed for imaging multiple low-abundance miRNAs in living cells. It offers improved stability, efficient delivery, and amplified signals for sensitive miRNA detection.
JOURNAL OF MATERIALS CHEMISTRY B
(2022)
Article
Chemistry, Analytical
Linyan Li, Xiaojun Fang, Jingqing Le, Yanhui Zheng, Xiarong Tan, Zhou Jiang, Hongxia Li, Jianguo Xu, Huo Xu
Summary: In this study, a cascade catalytic hairpin assembly (CCHA) method was developed for sensitive and selective detection of miRNA. The method showed high assay sensitivity, excellent sequence specificity, and the ability for intracellular imaging, demonstrating promising potential for biosensing, clinical diagnosis, or prognosis.
Article
Chemistry, Multidisciplinary
Qingqing Zhang, Shanshan Yu, Jinhua Shang, Shizhen He, Xiaoqing Liu, Fuan Wang
Summary: To improve the sensing performance of DNAzymes in live cells, a compact and robust Zn@DDz nanoplatform was developed. This platform enables amplified intracellular imaging of microRNA by controlling the disassembly of Zn@DDz nanoparticles. This work provides a promising approach for DNAzyme-based biosensing in living cells.
Article
Chemistry, Multidisciplinary
Zhaoyu Ma, Mohamed F. Foda, Huageng Liang, Yanli Zhao, Heyou Han
Summary: The discovery of near-IR-II (NIR-II) tumor phototheranostics uses nanozyme-augmented NIR-II agents for specific tumor ablation, providing a promising all-in-one approach for tumor therapeutics without harming normal tissues.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Article
Biophysics
Xia Cheng, Dandan Ren, Guanhong Xu, Fangdi Wei, Jing Yang, Jian Xu, Lin Wang, Qin Hu, Yao Cen
Summary: A metal-organic frameworks-assisted nonenzymatic cascade amplification strategy was developed for simultaneous quantification of three AMI-related miRNAs, showing high sensitivity and promising potential for improving the accuracy of clinical early AMI diagnosis.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Biophysics
Kejun Dong, Wei Zhang, Hao Hu, Shuangshuang Cheng, Yaoqin Mu, Bei Yan, Wan Shu, Longjie Li, Hongbo Wang, Xianjin Xiao
Summary: This study innovatively invented self-amplified dual-input synthetic genetic circuit nano-vehicle, which provides a solution to the compromise between specificity and sensitivity, and demonstrated its potential application in tumor recognition and treatment through experiments.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Multidisciplinary
Wei Zhu, Er-Li Cai, Hao-Zheng Li, Ping Wang, Ai-Guo Shen, Juergen Popp, Ji-Ming Hu
Summary: By designing small molecular monomers containing large Raman scattering cross-sections and polymerizing them at the nanoscale, smaller but brighter optical nanotags have been developed for high-throughput multi-color biomedical imaging, offering a new method for generating high sensitivity SRS imaging tags.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Chemistry, Multidisciplinary
Jia Ma, Daoxia Guo, Xiaoyuan Ji, Yanfeng Zhou, Chang Liu, Qian Li, Jiye Zhang, Chunhai Fan, Haiyun Song
Summary: It is found that hollow mesoporous CuS nanoparticles have the ability to inhibit GPX4. When these nanoparticles are loaded with an inhibitor of FSP1, they block two parallel redox systems and reinforce ICD through near-infrared irradiation. Furthermore, a hydrogel delivering CuS nanoparticles targeting cancer cells and SSO targeting immunosuppressive cells is fabricated, which enhances ICD and reinstates immune perception through lipid metabolic reprogramming, thereby triggering robust innate and adaptive immunity to restrain tumor growth, relapse, and metastasis. This study provides an immunometabolic therapy via orchestrated lipid modulation in the tumor milieu.
ADVANCED MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Miao Mao, Zhun Lin, Liang Chen, Zhengyu Zou, Jie Zhang, Quanhao Dou, Jiacheng Wu, Jinglin Chen, Minhao Wu, Li Niu, Chunhai Fan, Yuanqing Zhang
Summary: By constructing modular DNA-origami-based nanoarrays, we improved the binding affinity of surface proteins by matching the spatial distribution of target protein clusters. The nanoarrays significantly enhanced the binding affinity of target cells and synergistically recognized low-affinity antigen-specific cells. Furthermore, the DNA nanoarrays successfully verified their precise recognition ability and high-affinity RLIs in the clinical detection of circulating tumor cells.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Multidisciplinary Sciences
Yinan Zhang, Xiaoyao Yin, Chengjun Cui, Kun He, Fei Wang, Jie Chao, Tao Li, Xiaolei Zuo, Ailing Li, Lihua Wang, Na Wang, Xiaochen Bo, Chunhai Fan
Summary: In this study, a model based on DNA origami frameworks (DOFs) is designed to solve the prime factorization problem. The model uses DX tiles to assemble prime and composite integers, and the result is visualized using atomic force microscopy. This DNA-based parallel computing strategy has the potential to solve complex mathematical puzzles like prime factoring.
Review
Multidisciplinary Sciences
Zhimei He, Kejun Shi, Jinggang Li, Jie Chao
Summary: Since the early 1980s when Ned Seeman introduced the concept of DNA Holliday junction, the field of DNA nanotechnology has experienced significant development. DNA origami, in particular, has revolutionized DNA nano-technology by creating intricate structures with nanoscale precision using Watson-Crick base pairing. With its high programmability and addressability, DNA origami has emerged as a versatile nanomachine for various applications such as transportation, sensing, and computing. This review provides a brief overview of recent progress in DNA origami, two-dimensional patterning, and three-dimensional assembly, as well as its applications in nanofabrication, biosensing, drug delivery, and computational storage. The prospects and challenges in DNA origami assembly and application are also discussed.
Article
Chemistry, Multidisciplinary
Lele Sun, Fengyun Shen, Zijian Xiong, Yu Chao, Chunhai Fan, Zhuang Liu
Summary: Precisely organized antibody arrangements using DNA origami can enhance T cell activation and expand CD8+ T cells for tumor immunotherapy.
Article
Chemistry, Multidisciplinary
Meiyuan Qi, Wenhe Ma, Qin Xu, Fei Wang, Ping Song, Sisi Jia, Xiaolei Zuo, Mingqiang Li, Guangbao Yao, Chunhai Fan
Summary: Dynamic molecular interactions in chemical reaction networks lead to complex behaviors in living systems. Programming DNA molecular reactions at molecular and nanometer scales have been achieved, but achieving programmable autonomous behavior at submicron or larger scales remains challenging. This study presents a mechanism of meta-DNA strand displacement reactions (M-SDRs) mediated solely by meta-toehold (M-toehold) using versatile submicron building blocks of meta-DNA (M-DNA). M-SDR emulates the toehold binding and branch migration processes and shows potential for programming autonomous behavior at the cellular level.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Chemistry, Multidisciplinary
Kun Dai, Yang Xu, Yang Yang, Jianfeng Shen, Xiaoguo Liu, Xinyi Tu, Lu Yu, Xiaodong Qi, Jiang Li, Lihua Wang, Xiaolei Zuo, Yingbin Liu, Hao Yan, Chunhai Fan, Guangbao Yao
Summary: This study utilizes single-stranded RNA (ssRNA) origami as ligands for nucleic acid-sensing receptors, achieving improved stability, targeting, and immunogenicity prediction. The ssRNA self-folds into compact nanoparticles with defined shapes and exhibits resistance against degradation in cells. It can activate macrophages and neutrophils in the tumor microenvironment, leading to anti-tumoral immune response and tumor growth retardation in a mouse model. This ssRNA origami strategy provides a new solution for ligand design and biomedical applications of nucleic acid sensors.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Review
Chemistry, Analytical
Lihui Yuwen, Shifeng Zhang, Jie Chao
Summary: This paper reviews the history, principles, and applications of DNA nanobiosensors in virus detection. Compared to traditional virus detection methods, DNA nanobiosensors have higher sensitivity and specificity, and lower time and cost. However, challenges still exist for DNA nanobiosensors in virus detection, and further development and improvement are needed.
Article
Chemistry, Analytical
Jianfeng Ma, Lin Feng, Jie Li, Dan Zhu, Lianhui Wang, Shao Su
Summary: As a gold standard biomarker, cTnI is widely used for the diagnosis of AMI. In this study, a point-of-care electrochemical aptasensor was developed for facile and fast cTnI detection using screen-printed carbon electrodes and aptamers. The aptasensor showed a wide linear range and low detection limit for cTnI detection, with high selectivity and stability. Importantly, the aptasensor could be efficiently used with a mobile phone and a simple app for point-of-care testing, showcasing its promising potential.
Review
Chemistry, Analytical
Renqiang Yuan, Jing Cai, Haojie Ma, Yi Luo, Lianhui Wang, Shao Su
Summary: This review provides a comprehensive summary of recent advances in electrochemical aptasensors, including their construction methods, advantages, and applications in detecting various chemical and biological molecules. The prospects and challenges associated with this technology are also discussed.
Article
Chemistry, Analytical
Dan Zhu, Jiaxuan Huang, Yanting Xia, Shao Su, Xiaolei Zuo, Qian Li, Lianhui Wang
Summary: A novel multicolor DNAzymes-embedded framework nucleic acids (FNAzymes) were developed for simultaneous and quantitative detection of intracellular Cu2+ and Zn2+. The FNAzymes, with high uniformity and structural accuracy, can enter cells and achieve non-destructive, semi-quantitative detection of metal ions in living cells. This proposed method provides new opportunities for multi-target detection with low-nanomolar sensitivity.
Article
Computer Science, Artificial Intelligence
Yan Zhao, Shuting Cao, Yue Wang, Fan Li, Lixuan Lin, Linjie Guo, Fei Wang, Jie Chao, Xiaolei Zuo, Ying Zhu, Lihua Wang, Jiang Li, Chunhai Fan
Summary: The environments in living cells are highly heterogeneous and compartmentalized, posing a grand challenge for the deployment of theranostic agents with spatiotemporal precision. Despite rapid advancements in creating nanodevices responsive to various cues in cellular environments, it remains difficult to control their operations based on the temporal sequence of these cues.
NATURE MACHINE INTELLIGENCE
(2023)
Article
Chemistry, Multidisciplinary
Yao Luo, Min Yin, Chunlan Mu, Xingjie Hu, Hui Xie, Jingyi Li, Tingting Cao, Nan Chen, Ji Wu, Chunhai Fan
Summary: This study developed a nanocomplex, Pdot-siRNA, based on semiconductor polymer dots for effective gene knockdown in female germline stem cells (FGSCs). The high fluorescence brightness of Pdots was utilized for comprehensive investigation of their cellular uptake, intracellular trafficking, and exocytosis in FGSCs. Furthermore, Pdots demonstrated excellent biocompatibility and minimal disturbance to FGSCs differentiation. The intracellular Pdots could escape from lysosomes and undergo active exocytosis, making them ideal nanocarriers for bioactive cargos. Additionally, Pdot-siRNA could penetrate into 3D ovarian organoids derived from FGSCs and down-regulate the expression levels of target genes. This study sheds light on the manipulation and medical application of FGSCs and explores the interface between theranostic nanoparticles and FGSCs for the first time.
ADVANCED MATERIALS
(2023)
Review
Biochemical Research Methods
Yuan Luo, Zhen Cao, Yifan Liu, Rong Zhang, Shijia Yang, Ning Wang, Qingyuan Shi, Jie Li, Shurong Dong, Chunhai Fan, Jianlong Zhao
Summary: DNA has shown great potential as an alternative for digital information storage but is currently hindered by high material cost, time consumption for data reading/writing, and lack of an integrated system. Microfluidics has emerged as a promising solution because of its ability to handle and process micro-scale fluid samples in a highly integrated manner. This review discusses recent efforts and advancements in applying microfluidics to DNA data storage, highlighting its potential and future directions for integration and real-life applications.
Article
Biotechnology & Applied Microbiology
Kaidi Wang, Jing Chen, Jay Martiniuk, Xiangyun Ma, Qifeng Li, Vivien Measday, Xiaonan Lu
Summary: Reliable typing of yeast strains is crucial for the alcoholic beverage industry, and this study proposes a method using Raman spectroscopy and CNN for species identification and strain discrimination of S. cerevisiae and S. uvarum. The results demonstrate high accuracy and fast speed, providing significant implications for improving the quality of fermented beverages and conducting high-throughput screening.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
