Article
Chemistry, Applied
Hyoyoung Kim, Eun Joo Baek, Yong-Kyoung Kim, Hyejin Park, Suel Hye Hur, Jang-Eok Kim, Ho Jin Kim
Summary: In this study, a specific, reliable, and suitable method for a fast and simultaneous analysis of 335 pesticides in ginseng was developed using liquid chromatography quadrupole mass spectrometry (LC-MS/MS) and gas chromatography quadrupole mass spectrometry (GC-MS/MS). The method was validated for linearity, sensitivity, selectivity, accuracy, and precision, with limits of detection (LOD) and limits of quantification (LOQ) ranging from 0.1-5.8 μg/kg and 0.3-17.5 μg/kg, respectively. Analysis of 467 ginseng samples from 2016 to 2019 revealed pesticide residues in 304 samples, but most were below the standard. The hazard quotient (HQ) of ginseng for detected pesticides was less than 1, indicating low risk.
Article
Chemistry, Analytical
Amanda L. Burkhart, Ruth Waddell Smith, Victoria L. McGuffin
Summary: A kinetic model was developed to predict evaporation of compounds based on gas chromatographic retention index. Experimental determination of rate constants for highly volatile compounds allowed for extension of the model's applicability range with successful validation through comparison of predicted and experimental chromatograms.
ANALYTICA CHIMICA ACTA
(2021)
Article
Environmental Sciences
Na-Rae Choi, Yong-Jun Yoon, Ki-Tae Park, Ki-Ae Kim, Yong-Pyo Kim, Yun-Gyong Ahn, Ji-Yi Lee
Summary: This study utilized GC-MS/MS technology to analyze saccharides in pristine marine aerosols, finding that the detection limits were lower compared to conventional GC-MS, improving the time resolution for sampling saccharides.
Article
Medical Laboratory Technology
Songlin Yu, Danchen Wang, Xiaoli Ma, Yuanyuan Zhang, Dandan Sun, Yutong Zou, Jialei Yu, Fang Zhang, Guohua Yang, Shaowei Xie, Qian Cheng, Xinqi Cheng, Ling Qiu
Summary: A LC-MS/MS method was established to quantify G17 and G34 simultaneously, showing high analytical performance. The method was compared with CLIA and RIA, and it was found to be highly correlated with both methods. The study suggests that G17 may be the most specific biomarker for gastrinoma diagnosis.
CLINICAL CHEMISTRY
(2021)
Article
Chemistry, Applied
Olanrewaju Sam Olayeriju, Adele Papetti, Raffaella Colombo, Barbara Mannucci, Mary Tolulope Olaleye, Akintunde Afolabi Akindahunsi
Summary: The phytochemical constituents in the aqueous methanolic leaf extract of Triclisia gilletii responsible for its nephroprotective potentials against ethane-1,2-diol induced nephrolithiasis were elucidated. Phenols, fatty acids, vitamins, and steroids were detected using both gas chromatography and liquid chromatography. Kaempferol and dihydrovomifoliol-O-glucoside were also identified. These constituents contribute to the pharmacological potential of Triclisia gilletii against ethane-1,2-diol induced nephrolithiasis.
NATURAL PRODUCT RESEARCH
(2022)
Article
Chemistry, Applied
Peng Sun, Zhao Yu, Chao Wang, Shuo Li
Summary: In this study, the content of eight sterols in rapeseed, including cholesterol, cholestanol, brassicasterol, ergosterol, campesterol, stigmasterol, 8-sitosterol, and stigmastanol, was determined using pressurized liquid extraction coupled with gas chromatography-mass spectrometry. The results showed a good linear relationship between the peak area and the corresponding mass concentration of the eight sterols. The developed method was efficient, simple, and accurate, suitable for the qualitative and quantitative analysis of sterols in rapeseed.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2023)
Article
Chemistry, Analytical
Sophia A. Schreckenbach, Denina Simmons, Adam Ladak, Lauren Mullin, Derek C. G. Muir, Myrna J. Simpson, Karl J. Jobst
Summary: This study developed a suspect screening method based on industrial compounds with bioaccumulation potential. Results showed that SQDIA has a significantly lower false-positive rate compared to standard DIA. Through suspect screening and nontargeted screening, multiple pollutants were identified, with some compounds confirmed through standards and structural analysis.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Magdalena Slowik-Borowiec, Ewa Szpyrka, Paulina Ksiazek-Trela, Magdalena Podbielska
Summary: New analytical approaches were developed for the identification and quantification of pesticides and PAHs in five representative matrices. The optimized procedure showed satisfactory results for all analytes, with improved recovery and precision. The method was successfully applied to determine pesticide residues in commercial samples and showed potential for reducing sample preparation time and solvent consumption.
Review
Medical Laboratory Technology
Kenichi Tamama
Summary: Drugs of abuse testing is commonly used in clinical and forensic settings, with urine being the preferred specimen type but other emerging types include saliva, sweat, hair, and meconium. Liquid chromatography-tandem mass spectrometry (LC-tandem-MS) has become the new gold standard for confirmatory drug testing due to its ability to analyze a wider range of analytes compared to gas chromatography-mass spectrometry (GC-MS). Technical advancements such as high-resolution mass spectrometry and miniature ambient ionization mass spectrometry have improved the speed and efficiency of drugs of abuse testing.
CLINICA CHIMICA ACTA
(2021)
Article
Multidisciplinary Sciences
Cara Olivier, Bianca Allen, Laneke Luies
Summary: This study optimized a urine preparation procedure for non-targeted metabolomics using GC-MS. The direct analysis method showed superior repeatability and higher metabolome coverage compared to the organic acid methods. The findings contribute to the advancement of urine metabolomics research.
SCIENTIFIC REPORTS
(2023)
Article
Food Science & Technology
Liyuan Wang, Zhengyan Hu, Jing Chen, Tianjiao Wang, Pinggu Wu, Ying Ying
Summary: This study developed a method to simultaneously detect a dozen preservatives in pastry using gas chromatography-mass spectrometry. The preservatives showed good linearity within the range of 1.0-50 μg/mL, with detection limits of 0.04-2.00 mg/kg and quantification limits of 0.12-6.67 mg/kg. The method is suitable for the determination of 12 preservatives in pastry.
Article
Biochemical Research Methods
T. Mouskeftara, C. Virgiliou, G. Theodoridis, H. Gika
Summary: The sensitive and precise GC-MS/MS method was developed for quantifying a large number of organic acids in human urine, based on key metabolic intermediates. Extensively validated, it can find wide applicability in metabolomics for clinical or nutritional studies.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Review
Chemistry, Analytical
A. Fernez Asensio, E. Alvarez-Gonzalez, A. Rodriguez, L. M. Sierra, E. Blanco-Gonzalez
Summary: Dysregulation of TCA cycle enzymes leads to abnormal accumulation of oncometabolites, which contribute to cancer formation and progression. GC-MS and LC-MS are powerful analytical tools for accurate identification and determination of these metabolites.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Applied
Petar Ristivojevic, Vesna Jovanovic, Dusanka Milojkovic Opsenica, Jihae Park, Judith M. Rollinger, Tanja Cirkovic Velickovic
Summary: A novel high-throughput methodological approach was developed for tracing compounds with radical scavenging and antimicrobial activities in brown seaweeds. Major components identified with these activities include stearidonic, eicosapentaenoic, and arachidonic acids. This method allows fast identification and quantification of bioactive compounds in multicomponent biological samples.
Article
Food Science & Technology
Mark M. Skinner, Jared T. Seale, Maranda S. Cantrell, Joseph M. Collins, Matthew W. Turner, Owen M. McDougal
Summary: This experimental study aimed to detect acrylamide in French fries using methods suitable for the food processing industry, comparing the pros and cons of each method to provide decision-making suggestions for food processors.
Article
Microbiology
Mengyin Yao, Felix J. Elling, CarriAyne Jones, Sulung Nomosatryo, Christopher P. Long, Sean A. Crowe, Maciek R. Antoniewicz, Kai-Uwe Hinrichs, Julia A. Maresca
ENVIRONMENTAL MICROBIOLOGY
(2016)
Article
Cardiac & Cardiovascular Systems
Scott B. Crown, Joanne K. Kelleher, Rosanne Rouf, Deborah M. Muoio, Maciek R. Antoniewicz
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2016)
Article
Chemistry, Analytical
Brian O. McConnell, Maciek R. Antoniewicz
ANALYTICAL CHEMISTRY
(2016)
Article
Biotechnology & Applied Microbiology
Woo Suk Ahn, Scott B. Crown, Maciek R. Antoniewicz
METABOLIC ENGINEERING
(2016)
Article
Multidisciplinary Sciences
Troy E. Sandberg, Christopher P. Long, Jacqueline E. Gonzalez, Adam M. Feist, Maciek R. Antoniewicz, Bernhard O. Palsson
Article
Multidisciplinary Sciences
Shawn W. Jones, Alan G. Fast, Ellinor D. Carlson, Carrissa A. Wiedel, Jennifer Au, Maciek R. Antoniewicz, Eleftherios T. Papoutsakis, Bryan P. Tracy
NATURE COMMUNICATIONS
(2016)
Article
Oncology
Santiago Diaz-Moralli, Esther Aguilar, Silvia Marin, Johannes F. Coy, Mieke Dewerchin, Maciek R. Antoniewicz, Oscar Meca-Cortes, Leen Notebaert, Bart Ghesquiere, Guy Eelen, Timothy M. Thomson, Peter Carmeliet, Marta Cascante
Article
Multidisciplinary Sciences
Christopher P. Long, Jacqueline E. Gonzalez, Adam M. Feist, Bernhard O. Palsson, Maciek R. Antoniewicz
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2018)
Article
Biochemical Research Methods
Christopher P. Long, Maciek R. Antoniewicz
Article
Biotechnology & Applied Microbiology
Robert Kyle Bennett, Alec Agee, Jie R. G. Har, Bryan von Hagel, Maciek R. Antoniewicz, Eleftherios T. Papoutsakis
Summary: This study identified a key limitation of autonomous methylotrophy in E. coli and successfully improved biosynthesis of limiting amino acids from methanol carbon by modifying global and local metabolic networks, leading to enhanced growth in methanol minimal medium.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Biotechnology & Applied Microbiology
Jie Ren Gerald Har, Alec Agee, R. Kyle Bennett, Eleftherios T. Papoutsakis, Maciek R. Antoniewicz
Summary: Recent studies have shown that bacteria can synthesize all amino acids from methanol without stoichiometric coupling, by evolving strains through adaptive laboratory evolution. These evolved strains efficiently produce amino acids from methanol and can achieve autonomous growth on methanol as the sole carbon source, offering potential for further engineering and adaptive evolution in the future.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Maciek R. Antoniewicz
Summary: Metabolic engineering aims to improve biological production by designing and optimizing metabolic pathways, quantifying metabolic fluxes, and analyzing metabolic network models; essential concepts include metabolic network models and metabolic fluxes; in recent years, advancements in technology and methods have allowed for a deeper understanding of the capabilities of biological systems.
METABOLIC ENGINEERING
(2021)
Article
Biotechnology & Applied Microbiology
Eleanor H. Oates, Maciek R. Antoniewicz
Summary: Adipose tissue plays a key role in regulating lipid and energy homeostasis by storing excess nutrients and releasing energetic substrates through lipolysis. Differentiated adipocytes undergo significant metabolic changes, including increased glucose uptake, redirection of glucose utilization, and increased pathway fluxes through glycolysis and oxidative pentose phosphate pathway.
METABOLIC ENGINEERING
(2022)
Article
Biotechnology & Applied Microbiology
Michael L. Dahle, Eleftherios T. Papoutsakis, Maciek R. Antoniewicz
Summary: This study used C-13 metabolic flux analysis to investigate the core metabolic pathways of acetogenic bacteria Clostridium ljungdahlii. The study found that autotrophic, heterotrophic, and mixotrophic growth can be achieved in a defined medium. The internally-produced carbon dioxide from the metabolism of asparagine and fructose was consumed by the Wood-Ljungdahl pathway, while externally supplied CO2 was the main carbon source. The study also revealed important findings about the production and utilization of ATP, NADH, and acetyl-CoA.
METABOLIC ENGINEERING
(2022)
Article
Biotechnology & Applied Microbiology
Eleanor H. Oates, Maciek R. Antoniewicz
Summary: Hypoxia has a significant impact on the physiology and metabolism of adipocytes, leading to the suppression of adipogenesis, de novo lipogenesis, and metabolic reprogramming. It alters the patterns of cellular metabolism, redirects glucose metabolism, rewires glutamine metabolism, suppresses branched chain amino acid consumption, and reduces the biosynthesis of certain fatty acids.
METABOLIC ENGINEERING
(2023)
