Article
Chemistry, Analytical
Jiawei Gong, Tongtong Zhang, Peng Chen, Fei Yan, Jiyang Liu
Summary: In this study, we constructed a dual-mode electrochemiluminescence and electrochemical sensing platform using bipolar silica nanochannel array. Through the modification of a bifunctional probe, we successfully achieved quantitative detection of specific analytes.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
Yuyang Huang, Liyun Deng, Di Su, Xiangyi Huang, Jicun Ren
Summary: The study presented a method based on FCS and PS Pdots for detecting DNA methyltransferase activity and inhibitor screening. The results demonstrate that the method has high sensitivity and reliability.
Review
Chemistry, Multidisciplinary
Yoshihiro Sohtome, Tadahiro Shimazu, Yoichi Shinkai, Mikiko Sodeoka
Summary: Developing synthetic strategies to construct covalent bonds is a common research topic among synthetic chemists. The success of such strategies relies on the tunability of conditions such as catalysts, reagents, solvents, and reaction temperature. However, controlling chemical reactions in living cells poses challenges due to a smaller number of hit substrates and a complex and inflexible environment. The use of chemical methylome analysis serves as an example of bridging the gap between chemistry and biology in this context.
ACCOUNTS OF CHEMICAL RESEARCH
(2021)
Article
Biophysics
Jessika F. F. Ribeiro, Jose R. S. Melo, Caroline de L. Santos, Claudilene R. Chaves, Paulo E. Cabral Filho, Goreti Pereira, Beate S. Santos, Giovannia A. L. Pereira, Daniela S. Rosa, Rogerio T. Ribeiro, Adriana Fontes
Summary: We developed a nanostructured electrochemical immunosensing platform using quantum dots and screen-printed carbon electrodes for the detection of the Zika virus envelope protein. The platform showed high sensitivity and specificity for EP-ZIKV detection.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2023)
Article
Chemistry, Applied
Ricarda Torre, Maria Freitas, Estefania Costa-Rama, Henri P. A. Nouws, Cristina Delerue-Matos
Summary: This study reports the development of an electrochemical immunosensor for detecting tropomyosin (TPM) in the muscles of crustacean species, a major shellfish allergen. The sensor showed good selectivity and accuracy in detecting TPM, enabling effective control of food labelling according to EU regulations.
Review
Pharmacology & Pharmacy
Zhixiong Zhang, Guan Wang, Yuyan Li, Dongsheng Lei, Jin Xiang, Liang Ouyang, Yanyan Wang, Jinliang Yang
Summary: DNA methylation is an important epigenetic process that regulates gene expression and has become a promising target for cancer treatment.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Chemistry, Analytical
Mingmin Wu, Mengtian Zhang, Zhiwei Fan, Xinyue Qin, Xiaoxia Zhu, Haiwei Ji, Yuling Qin, Qi Wang, Li Wu
Summary: Sensitive detection of DNA methyltransferase activity using a new fluorescence transducer based on Forster resonance energy transfer (FRET) is presented, with high analytical performance and capability for enzyme inhibitor screening. The method offers a promising strategy for high-performance diagnosis, drug discovery, and treatment effectiveness evaluation in various disease-related methyltransferase activities.
Article
Chemistry, Analytical
Simge Balaban, Tutku Beduk, Ceren Durmus, Eda Aydindogan, Khaled Nabil Salama, Suna Timur
Summary: In this study, a bio-platform for eIF3d sensing was constructed using LSG and surface functionalization with anti eIF3d antibody via EDC/NHS chemistry. XPS and various electrochemical methods were used to validate the sensor, showing sensitivity to eIF3d biomarker amounts between 75-500 ng/mL with a LOD of 50.4 ng/mL. The biosensor's selectivity was demonstrated through testing with interference molecules, and successful detection of EIF3d was achieved in synthetic biological samples, marking a rare use of LSGs in immunosensor studies.
Article
Chemistry, Analytical
Linfei Xiong, Zhaohui Li, Guangming Li, Huangxian Ju
Summary: This study developed a DNA dendrimer for loading signal molecules and constructing an amplified electrochemical immunosensing method. The DNA dendrimer was self-assembled by hybridizing complementary oligonucleotides, enabling signal amplification. The method showed excellent performance in analyzing prostate specific antigen as a model analyte.
ANALYTICA CHIMICA ACTA
(2021)
Article
Biochemistry & Molecular Biology
Tummala Anusha, Kalli Sai Bhavani, Rabeay Y. A. Hassan, Pradeep Kumar Brahman
Summary: A new ultrasensitive and low-cost electrochemical immunosensing probe was designed for monitoring vitamin D deficiency using 25(OH)D3 as a clinical biomarker. The probe utilized ferrocene carbaldehyde conjugated on Ab-25(OH)D3 antibodies as an electrochemical probe for signal generation. Graphene nanoribbon-modified electrode (GNRs) was employed for immobilizing the (Ab-25(OH)D3-Fc) conjugate, allowing for efficient capture of primary antibodies. The developed probe exhibited excellent sensitivity for 25(OH)D3 detection.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Multidisciplinary Sciences
Alex M. Yoshikawa, Alexandra E. Rangel, Liwei Zheng, Leighton Wan, Linus A. Hein, Amani A. Hariri, Michael Eisenstein, H. Tom Soh
Summary: Aptamer-based molecular switches have various applications such as imaging metabolites, drug delivery, and biomolecule detection. Conventional aptamer selection methods do not produce aptamers with structure-switching functionality, so post-selection modification is necessary. This study presents a screening-based strategy that can convert any aptamer into a molecular switch without prior knowledge of its structure.
NATURE COMMUNICATIONS
(2023)
Article
Chemistry, Multidisciplinary
Sneh M. Toprani, Dimitrios Bitounis, Qiansheng Huang, Nathalia Oliveira, Kee Woei Ng, Chor Yong Tay, Zachary D. Nagel, Philip Demokritou
Summary: The potential genotoxic effects of engineered nanomaterials (ENMs) on DNA repair pathways have been investigated using a fluorescence multiplex-host-cell reactivation (FM-HCR) assay. Results showed that some ENMs may enhance DNA repair capacity while others suppress it. This method can be a valuable part of a multitier, in vitro hazard assessment of ENMs, providing insights into the interplay between ENM properties, DNA repair efficiency, and genomic stability.
Article
Chemistry, Analytical
Dandan Hu, Di Wu, Yanmei Lu, Jiayue Liu, Zhiyong Guo, Sui Wang, Chunyang Zhai, Zhihe Qing, Yufang Hu
Summary: A new electrochemical biosensing method based on pH-induced DNA conformational change is reported for the detection of glucose oxidase (GOx) and urease. The platform exhibits good performance and has the potential for monitoring GOx or urease in practical biosystems and clinical medical examinations.
ANALYTICA CHIMICA ACTA
(2022)
Article
Environmental Sciences
Shahram Parvin, Pegah Hashemi, Abbas Afkhami, Mostafa Ghanei, Hasan Bagheri
Summary: Developing ultrasensitive assays for the detection of botulinum neurotoxin (BoNT) is crucial for human health and the surrounding environment. In this study, an electrochemical sandwich immunoassay was used to simultaneously detect BoNT serotypes A and E. The method showed high sensitivity, wide linear dynamic range, and good stability and reproducibility.
Article
Chemistry, Analytical
Wenxu Yuan, Kaiting Xiao, Xingxing Liu, Yanming Lai, Fazeng Luo, Wei Xiao, Jinjun Wu, Pan Pan, Yongkui Li, Heng Xiao
Summary: In this study, a colorimetric biosensor (PER-FHGD nanodevice) was developed to detect DNA MTase activity. The PER-FHGD system showed excellent sensitivity with a limit of detection (LOD) as low as 0.3 U/mL for Dam MTase, as well as remarkable selectivity and screening ability for Dam MTase inhibitors. Furthermore, this method successfully detected Dam MTase activity in both serum and E. coli cell extracts.
ANALYTICA CHIMICA ACTA
(2023)
Article
Nanoscience & Nanotechnology
Yu-Shi Liu, Xiang Wei, Xu Zhao, Li-Jian Chen, Xiu-Ping Yan
Summary: The study presents a smart nanoplatform with synergistic chemo-phototherapy function for targeted imaging and efficient sterilization of bacteria, with minimal side effects on normal tissues.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Chemistry, Multidisciplinary
Li-Jian Chen, Yao-Yao Liu, Xu Zhao, Xiu-Ping Yan
Summary: The modification of vancomycin on a porphyrinic metal-organic framework PCN-224 results in Van-PCN-224, which exhibits high photosensitive activity and excellent targeted antibacterial activity against Staphylococcus aureus. These features make Van-PCN-224 a promising candidate for antimicrobial therapy.
CHEMISTRY-AN ASIAN JOURNAL
(2021)
Article
Chemistry, Analytical
Yuan-Yuan Jiang, Xu Zhao, Li-Jian Chen, Cheng Yang, Xue-Bo Yin, Xiu-Ping Yan
Summary: This study presents a novel nanosensor for the simultaneous detection of AFB(1) and ZEN in food samples, utilizing dual-colored persistent luminescence nanoparticles and Fe3O4 magnetic nanoparticles. The nanosensor integrates features such as autofluorescence-free detection, high specificity, and rapid magnetic separation, enabling highly sensitive and selective detection of mycotoxins. The developed technology offers a universal optical platform for the simultaneous detection of multiple contaminants in complex samples.
Article
Chemistry, Analytical
Lu-Ming Pan, Xu Zhao, Xiang Wei, Li-Jian Chen, Chan Wang, Xiu-Ping Yan
Summary: A ratiometric luminescence aptasensor based on dual-emissive persistent luminescent nanoparticles (PLNP) was developed for the accurate determination of trace aflatoxin B1 (AFB1) in complex food samples. The aptasensor exhibited excellent linearity, low limit of detection, and good precision. It also showed high selectivity and was successfully applied for the determination of AFB1 in various food samples with satisfactory recoveries.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Physical
Bei-Bei Wang, Li-Xia Yan, Li-Jian Chen, Xu Zhao, Xiu-Ping Yan
Summary: This study reports a multifunctional nanoplatform that can effectively kill bacteria and provide high-sensitive persistent luminescence turn-on imaging by triggering the bacterial microenvironment. The platform enables accurate targeting, high sensitivity imaging, synergistic therapy, and on-demand drug release for multidrug-resistant bacterial infections.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2022)
Article
Chemistry, Applied
Dong-Hui Wang, Li-Jian Chen, Xu Zhao, Xiu-Ping Yan
Summary: We successfully designed a photosensitizer with dual-organelle targeting and dual-color emission by introducing cyano groups. This photosensitizer can light up the mitochondria, locate into the lysosome and nucleolus, and self-report cell death by observing the change of emission color.
Article
Chemistry, Applied
Jing-Xuan Guo, Lu-Ming Pan, Meng-Chao Wang, Li-Jian Chen, Xu Zhao
Summary: Here, we reported a ratiometric aptasensor based on dual-colored persistent luminescent nanoparticles for accurate detection of ochratoxin A (OTA), which was free from exogenous interference and autofluorescence. The developed aptasensor showed a low detection limit of 3.4 pg mL-1, wide linearity in the range of 0.01-50 ng mL-1, and high precision of 3.1%. It was successfully applied for the analysis of OTA in grain samples with recoveries of 97.6%-105.2%.
Article
Materials Science, Biomaterials
Meng-Chao Wang, Jing-Xuan Guo, Li-Jian Chen, Xu Zhao
Summary: The research team successfully developed a multifunctional porphyrin-COF for targeted bacteria and synergistic phototherapy/chemotherapy to achieve sterilization and wound healing. The ordered crystal structure of the porphyrin-COF effectively avoids fluorescence quenching and facilitates the storage and transport of singlet oxygen. The acrylate substituent in the COF serves as a bacterial targeting moiety and reacts with the bacterial surface protein, fixing the bacteria on the COF surface and inhibiting colonization. The bonding of COF and bacteria further enhances the therapeutic efficiency of phototherapy.
BIOMATERIALS SCIENCE
(2023)
Article
Nanoscience & Nanotechnology
Li-Ya Wang, Li-Jian Chen, Xu Zhao, Yan Lv, Tianxi Liu, Xiu-Ping Yan
Summary: The researchers report an activatable antimicrobial nanoplatform with ultralow-background persistent luminescent (PL) turn-on imaging and therapeutic capability. The platform utilizes chitosan as a linker to immobilize copper ions on the surface of persistent luminescent nanoparticles (PLNPs) to fabricate PLNPs-CS-Cu2+. The PL emission of PLNPs is quenched by Cu2+ under physiological conditions but recovered due to the conversion of Cu2+ into Cu+ by high glutathione (GSH) levels at the infection site. The as-prepared antibacterial nanoplatform shows promise for highly sensitive persistent luminescent imaging and simultaneous treatment of bacterial infection.
ACS APPLIED NANO MATERIALS
(2023)
Article
Materials Science, Multidisciplinary
Xu Zhao, Ting-Jiao Shi, Yao-Yao Liu, Li-Jian Chen
Summary: Food-borne diseases caused by fresh fruits and vegetables pose a serious threat to human health, necessitating effective antibacterial measures. Incorporating highly efficient photosensitive MOF-545 nanorods into PCL material to prepare antibacterial composite films has shown strong antibacterial activity under white LED light, offering a new option for antibacterial packaging of fruits and vegetables.
ACS APPLIED POLYMER MATERIALS
(2022)
Article
Chemistry, Applied
Jiang-Yue Wang, Li-Jian Chen, Xu Zhao, Xiu-Ping Yan
Summary: A pH-responsive colorimetric microneedle (MN) patch made from bromothymol blue (BTB) and silk fibroin meth acryloyl (SilMA) was developed for sensing salmon spoilage. The MN patch could extract tissue fluids from salmon and display color changes based on the pH variation caused by the increase of total volatile basic nitrogen. This method allows rapid determination of salmon spoilage and can be analyzed nondestructively on a smartphone.
Article
Nanoscience & Nanotechnology
Jia-Hua Gong, Li-Jian Chen, Xu Zhao, Xiu-Ping Yan
Summary: This study reports the preparation of Zn2GeO4:Cu2+ persistent luminescence nanorods and their application in treating MRSA-infected wounds. ZGC shows a long-persistent photocatalytic effect, continuously producing multiple ROS without the need for in situ excitation. ZGC@MNs patches exhibit excellent antibacterial activity and biocompatibility for promoting wound healing.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Materials Science, Biomaterials
Xu Zhao, Xiang Wei, Li-Jian Chen, Xiu-Ping Yan
Summary: Bacterial infections pose a serious threat to public health, and finding a precise sterilization method is crucial. This study reports an intelligent theranostic platform that can be activated specifically by bacteria, combining photodynamic therapy and chemodynamic therapy to achieve precise sterilization and wound healing.
BIOMATERIALS SCIENCE
(2022)
Article
Materials Science, Biomaterials
Dong-Hui Wang, Li-Jian Chen, Xu Zhao, Xiu-Ping Yan
Summary: This study presents a novel self-reporting photosensitizer TPA-3PyA+ for photodynamic therapy and real-time monitoring, featuring dual-color emissions to self-report cell death effectively.
JOURNAL OF MATERIALS CHEMISTRY B
(2021)
Article
Nanoscience & Nanotechnology
Yi Zhang, Xu-Fang Qu, Cheng-Long Zhu, Han-Jie Yang, Chen-Hui Lu, Wen-Long Wang, Yuehong Pang, Cheng Yang, Li-Jian Chen, Xing-Fang Li
Summary: Quaternized chitosan-modified black phosphorus nanosheets (BP-QCS) exhibit enhanced solubility and stability in aqueous suspensions, showing synergistic antibacterial effects under near-infrared irradiation and negligible cytotoxicity. This composite material has potential for use in antibacterial therapy, with promising results in both in vitro and in vivo studies.
ACS APPLIED BIO MATERIALS
(2021)