Article
Chemistry, Medicinal
Maho Takioku, Yuta Takamura, Michiko Fujihara, Masaki Watanabe, Shoya Yamada, Mayu Kawasaki, Sohei Ito, Shogo Nakano, Hiroki Kakuta
Summary: This study found that RXR antagonist CBTF-EE may bind in different modes depending on the presence of nitrobenzoxadiazole or boron-dipyrromethene fluorophores at the end of the alkoxy chain. Fluorescence assays and docking simulations supported the conclusion that the binding modes of these compounds varied.
ACS MEDICINAL CHEMISTRY LETTERS
(2021)
Review
Chemistry, Analytical
Saimon M. M. Silva, Miaosi Li, Alexandre Xavier Mendes, Simon E. E. Moulton
Summary: The use of reagentless protein-based biosensors has the potential to revolutionize our understanding of biology and personalized health monitoring by directly monitoring molecular analytes in bodily fluids. Currently available molecular sensors are limited in their applicability to relevant biomarkers due to their reliance on specific enzymatic or chemical reactivity. A new class of reagentless protein-based electrochemical sensors has been introduced to overcome this limitation and enable direct measurements of biomarkers in unprocessed biological fluids. This mini-review discusses recent advances in the development of electroanalytical modular biosensors that integrate all the necessary components for autonomous functionality.
Article
Multidisciplinary Sciences
Yan Yan, Somnath Mukherjee, Kaleeckal G. Harikumar, Timothy S. Strutzenberg, X. Edward Zhou, Kelly Suino-Powell, Ting-Hai Xu, Ryan D. Sheldon, Jared Lamp, Joseph S. Brunzelle, Katarzyna Radziwon, Abigail Ellis, Scott J. Novick, Irving E. Vega, Russell G. Jones, Laurence J. Miller, H. Eric Xu, Patrick R. Griffin, Anthony A. Kossiakoff, Karsten Melcher
Summary: AMPK regulates cellular metabolism by modulating AL phosphorylation and accessibility, with ATP inhibiting its activity when levels are low. Researchers have developed antibodies and analyzed structures to reveal the mechanism by which adenine nucleotides and pharmacological agonists regulate AMPK activity.
Article
Biochemistry & Molecular Biology
Valentina Pagliara, Giuseppina Amodio, Vincenzo Vestuto, Silvia Franceschelli, Nicola Antonino Russo, Vittorio Cirillo, Giovanna Mottola, Paolo Remondelli, Ornella Moltedo
Summary: ATF6a is an endoplasmic reticulum protein involved in unfolded protein response in mammals. In differentiating mouse C2C12 myoblasts, only the ATF6a pathway of UPR is activated. Activation of ATF6a depends on p38 MAPK and its inhibition impairs myotube formation and trafficking of ATF6a.
Article
Biochemistry & Molecular Biology
Jing-Ting Chiou, Yi-Jun Shi, Yuan-Chin Lee, Liang-Jun Wang, Ying-Jung Chen, Long-Sen Chang
Summary: SEM-LA activates TNF-α-mediated apoptosis in U937 cells through the NOX4/p38 MAPK/PP2A axis and promotes this process via Ca2+-NOX4-ROS axis-mediated p38 MAPK activation. Restoration of TTP expression alleviates the effects of TNF-α upregulation and cell death induced by SEM-LA.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Chemistry, Medicinal
Shiyan Zhang, Ziqin Yan, Yafang Li, Yang Gong, Xilin Lyu, Jianfeng Lou, Daizhou Zhang, Xiangjing Meng, Yujun Zhao
Summary: A new small-molecule inhibitor, 31 (YL93), was designed and synthesized to target both MDM2 and MDM4, resulting in stronger antitumor activities in wild-type p53 and MDM4-overexpressing cancer cells.
JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Biology
Yingyu Zhou, Tomohiro Takano, Xuyang Li, Yimei Wang, Rong Wang, Zhangliang Zhu, Masaru Tanokura, Takuya Miyakawa, Satoshi Hachimura
Summary: Beta-elemene regulates the balance of proinflammatory cytokines in mouse white adipose tissue through MAPK signaling, affecting the imbalance of M1-M2 macrophages and inhibiting the phosphorylation of MAPK pathways. These results suggest that beta-elemene may be a potential therapeutic medicine for macrophage-mediated chronic diseases.
COMMUNICATIONS BIOLOGY
(2022)
Article
Biology
Alejandra Escos, Ester Diaz-Mora, Michael Pattison, Pilar Fajardo, Diego Gonzalez-Romero, Ana Risco, Jose Martin-Gomez, Eric Bonneil, Nahum Sonenberg, Seyed Mehdi Jafarnejad, Juan Jose Sanz-Ezquerro, Steven C. Ley, Ana Cuenda
Summary: Evidence implicating p38? and p38d in inflammation is mainly based on experiments using Mapk12/Mapk13-deficient mice. A new mouse model, p38?/dKIKO, with specific depletion of p38? and p38d was generated to study their functions. The results showed reduced inflammation, susceptibility to septic shock, and Candida albicans infection in p38?/dKIKO mice compared to wild-type mice. Gene expression and phosphorylation analyses revealed the involvement of p38?/p38d in regulating innate immune responses through MEF2D phosphorylation and transcriptional activity.
Article
Infectious Diseases
Shuai-Bing Dong, Di Xiao, Jing-Yao Liu, Hui-Mei Bi, Zun-Rong Zheng, Li-Da Wang, Xiao-Wen Yang, Guo-Zhong Tian, Hong-Yan Zhao, Dong-Ri Piao, Zhi-Feng Xing, Hai Jiang
Summary: This study evaluated the value of Fluorescence Polarization Immunoassay (FPA) for the diagnosis of human brucellosis in China by recruiting 320 suspected cases. FPA showed high diagnostic value with a cutoff value of 88.5 mP, and when combined with ELISA, the sensitivity of diagnosis was significantly improved. FPA may have potential as a diagnostic method for human brucellosis in China.
INFECTIOUS DISEASES OF POVERTY
(2021)
Article
Biochemistry & Molecular Biology
Simon Kwoon Ho Chow, Can Cui, Keith Yu Kin Cheng, Yu Ning Chim, Jinyu Wang, Carissa Hing Wai Wong, Ka Wai Ng, Ronald Man Yeung Wong, Wing Hoi Cheung
Summary: The study found that low-magnitude high-frequency vibration can improve the inflammatory response at the fracture site by activating the p38 MAPK pathway, but does not directly affect the polarization of macrophages. This suggests that p38 MAPK regulates the enhancement effects of mechanical stimulation in vivo, while the exact mechanism may involve further investigation into different subsets of immune cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Olivia Molinar-Inglis, Jacob M. Wozniak, Neil J. Grimsey, Lennis B. Orduna-Castillo, Norton Cheng, Ying Lin, Monica L. Gonzalez Ramirez, Cierra A. Birch, John D. Lapek, David J. Gonzalez, JoAnn Trejo
Summary: This study identified candidate proteins regulated by PAR1-p38 signaling that may play important roles in endothelial dysfunction, by analyzing the global phosphoproteome in human cultured endothelial cells.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Cardiac & Cardiovascular Systems
Katharina Bottermann, Lisa Kalfhues, Rianne Nederlof, Anne Hemmers, Lucia M. Leitner, Vici Oenarto, Jana Nemmer, Mirjam Pfeffer, Vidisha Raje, Rene Deenen, Patrick Petzsch, Heba Zabri, Karl Koehrer, Andreas S. Reichert, Maria Grandoch, Jens W. Fischer, Diran Herebian, Johannes Stegbauer, Thurl E. Harris, Axel Goedecke
Summary: The study found that p38 MAPK alpha plays a crucial role in alleviating metabolic dysfunction and inflammation during cardiac adaptation to pressure overload. Lipolysis in adipose tissue and lipid accumulation in cardiomyocytes exacerbate cardiac inflammation, with neutrophils particularly triggering rapid left ventricular dilation.
BASIC RESEARCH IN CARDIOLOGY
(2022)
Article
Entomology
Huiting Zhao, Zhu Peng, Li Huang, Shuguo Zhao, Miaomiao Liu
Summary: This study focused on the classic OBP AcerOBP6 from the Asian honeybee, showing its high expression in the antennae of forager bees and extensive binding affinity with various odors, especially linolenic acid. The research provides insights into the olfactory recognition mechanism of A. cerana cerana.
Article
Chemistry, Medicinal
Kizuku Miura, Michiko Fujihara, Masaki Watanabe, Yuta Takamura, Mayu Kawasaki, Shogo Nakano, Hiroki Kakuta
Summary: This study measured the internal polarity of the RXR alpha-ligand binding pocket (LBP) by measuring fluorescence solvatochromism. The findings are expected to aid in the design of selective ligands for the RXR receptor. This method could also be applicable for evaluating the internal polarity of LBPs in other receptors.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2023)
Article
Biochemistry & Molecular Biology
Melissa Chasse, Neil Vasdev
Summary: Stress-activated kinases have potential as targets in neurodegenerative disease, and p38 alpha kinase in particular has shown promise as a druggable target. Researchers have successfully developed a PET radiotracer for imaging the MAPK p38 alpha/beta using the inhibitor talmapimod. Preclinical imaging studies in rodents demonstrated low brain uptake and retention, but pretreatment with a drug efflux inhibitor allowed the radiotracer to pass the blood-brain barrier. However, attempts to block the radiotracer binding with other inhibitors were unsuccessful. Further research is needed to develop p38 inhibitors that can avoid drug efflux and non-displaceable binding.
ACS CHEMICAL NEUROSCIENCE
(2023)
Article
Chemistry, Medicinal
Niklas M. Tormaehlen, Mariella Martorelli, Annette Kuhn, Florian Maier, Jamil Guezguez, Michael Burnet, Wolfgang Albrecht, Stefan A. Laufer, Pierre Koch
Summary: This study investigated the blood-brain barrier permeability of Skepinone-based p38 alpha MAP kinase inhibitors and their potential therapeutic effects in neuroinflammation and neurodegeneration. Methyl ester prodrugs were used to enhance oral absorption, resulting in compounds with good brain-to-plasma ratios. These compounds were selective for p38 alpha-MAP kinase, metabolically stable, and able to modulate IL-6 and TNF-alpha production.
JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Stephanie Cristine Hepp Rehfeldt, Joana Silva, Celso Alves, Susete Pinteus, Rui Pedrosa, Stefan Laufer, Marcia Ines Goettert
Summary: This study evaluated the neuroprotective, antioxidant, and anti-inflammatory activities of luteolin-7-O-glucoside (Lut7). The results suggest that Lut7 has neuroprotective effects and further studies should be conducted to validate its potential in the treatment of neurodegenerative diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Editorial Material
Chemistry, Medicinal
Stefan Laufer, Jurgen Bajorath, Matthias Gehringer, Nathanael Gray, Stephen Frye, Craig W. Lindsley
JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Cell Biology
Enni-Kaisa Mustonen, Tatu Pantsar, Azam Rashidian, Juliander Reiner, Matthias Schwab, Stefan Laufer, Oliver Burk
Summary: This study aimed to identify small-molecule kinase inhibitors that can also act as PXR antagonists. Through computational screening and experimental validation, four novel PXR antagonists and one agonist were identified. Further experiments showed that these compounds can directly bind to PXR and disrupt its interaction with coregulatory proteins.
Article
Chemistry, Medicinal
Michael Juchum, Bent Pfaffenrot, Philip Klovekorn, Roland Selig, Wolfgang Albrecht, Lars Zender, Stefan A. Laufer
Summary: MKK4 has been identified as a druggable target for the treatment of acute liver failure, and its inhibitors can promote liver regeneration or reduce hepatocyte death. However, the selectivity of existing potent inhibitors is highly sensitive to minor modifications of the molecule, requiring a careful balance between potency and selectivity.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Review
Pharmacology & Pharmacy
Darius P. Zlotos, Thales Kronenberger, Stefan A. Laufer
Summary: Hormone-dependent cancers, such as certain types of breast cancer, can be treated with anticancer drug conjugates that combine estrogen receptor (ER) ligands with other anticancer agents. These conjugates not only have dual action at anti-cancer targets, but also selectively deliver cytotoxic agents to ER-positive tumor cells, reducing toxicity and adverse effects. They can also overcome resistance to anti-hormonal monotherapy. This review discusses the design, structures, and pharmacological effects of various drug conjugates containing ER ligands linked to diverse anticancer agents.
Article
Pharmacology & Pharmacy
Thomas Pflieger, Rakesh Venkatesh, Markus Dachtler, Karin Eggenreich, Stefan Laufer, Dominique Lunter
Summary: This study applied SAOS rheology to investigate the formulation of the antihypertensive drug Metoprolol Succinate (MSN) in two carrier polymers for pharmaceutical 3D printing. The findings provided a better understanding of the extrudability and thermal durability of the pharmaceutical formulation, which is crucial for developing an approved printing process.
Article
Chemistry, Medicinal
Nadine Kruger, Thales Kronenberger, Hang Xie, Cheila Rocha, Stefan Pohlmann, Haixia Su, Yechun Xu, Stefan A. A. Laufer, Thanigaimalai Pillaiyar
Summary: The development of direct-acting antiviral drugs is necessary due to the COVID-19 pandemic caused by SARS-CoV-2. In this study, natural products were tested for their ability to inhibit the main protease M-pro of SARS-CoV-2. Some compounds were found to effectively inhibit M-pro and exhibited antiviral properties without cytotoxicity. The compound robinetin was able to form a covalent interaction with the catalytic site of M-pro.
Article
Chemistry, Medicinal
Jean Quancard, Anna Vulpetti, Anders Bach, Brian Cox, Stephanie M. Gueret, Ingo V. Hartung, Hannes F. Koolman, Stefan Laufer, Josef Messinger, Gianluca Sbardella, Russell Craft
Summary: Hit generation is a crucial step in drug discovery that determines the speed and success rate of identifying drug candidates. Different strategies are available for identifying chemical starting points for each biological target. This article discusses the best practices for target-centric hit generation, validation of hits, and the design of integrated strategies to maximize the chance of identifying high-quality starting points.
Review
Biochemistry & Molecular Biology
Leon Katzengruber, Pascal Sander, Stefan Laufer
Summary: MKK4 is a dual-specificity protein kinase that regulates JNK and p38 MAPK signaling pathways, impacting cell proliferation, differentiation, and apoptosis. It is overexpressed in aggressive cancer types and plays a key role in liver regeneration. MKK4 is a promising target for cancer therapeutics and the treatment of liver-associated diseases. Recent progress in MKK4 drug discovery and the interest from a startup company highlight its importance in drug development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Medicinal
Debora Bublitz Anton, Julia Galvez Bulhoes Pedreira, Maria Luiza Zvirtes, Stefan A. Laufer, Rodrigo Gay Ducati, Marcia Goettert, Luis Fernando Saraiva Macedo Timmers
Summary: The SARS-CoV-2 virus has caused a global pandemic, infecting over 688 million people and resulting in around 6.8 million deaths. The virus induces lung inflammation and the release of pro-inflammatory cytokines, making anti-inflammatory therapies important in addition to antiviral drugs. The main protease (MPro) of SARS-CoV-2 is a potential drug target as it is essential for viral replication. In this study, six kinase inhibitors were evaluated for their inhibitory activity against SARS-CoV-2 MPro, and BIRB-796 and Baricitinib were identified as potential inhibitors with dual antiviral and anti-inflammatory activity.
JOURNAL OF CHEMICAL INFORMATION AND MODELING
(2023)
Review
Chemistry, Medicinal
Valentin R. Wydra, Raphael B. Ditzinger, Nico J. Seidler, Frederik W. Hacker, Stefan A. Laufer
Summary: This article summarizes newly patented MAPK inhibitors from 2018 to early 2023, providing information on the patents, corresponding publications, development process, and clinical trials involving these compounds. Recently reported inhibitors show excellent selectivity and even achieve selectivity between closely related isoforms. This progress offers the possibility of eliminating unwanted side effects and may lead to the approval of the first MAPK inhibitor.
EXPERT OPINION ON THERAPEUTIC PATENTS
(2023)
Article
Biochemistry & Molecular Biology
Fatma Al-Rubaiai, Zakiya Zahran Al-Shariqi, Khalsa S. Al-Shabibi, John Husband, Asmaa M. Al-Hattali, Marcia Goettert, Stefan Laufer, Younis Baqi, Syed Imran Hassan, Majekodunmi O. Fatope
Summary: Maytenus dhofarensis Sebsebe is a shrub native to Oman that causes shivering attacks on grazed goats. Chemical investigation of its fruits and stems led to the discovery of new compounds, including a sesquiterpene pyridine alkaloid and lignanolactone. The structures and relative configurations of these compounds were determined through various analytical techniques. One of the compounds showed no inhibition activity on a kinase enzyme, while another compound exhibited strong antioxidant activity.
Article
Chemistry, Medicinal
Julian Laux, Mariella Martorelli, Simon Strass, Dieter Schollmeyer, Florian Maier, Michael Burnet, Stefan A. Laufer
Summary: This study investigated the distribution of small molecule pharmacology in blood, organs, and cells using fluorescent ligands. The results showed that the substance was mainly associated with granules or organelles, and azalide macrolides concentrated in lung and gut epithelia. These findings suggest that current estimation methods may underestimate the drug concentration in mucous membranes.
ACS PHARMACOLOGY & TRANSLATIONAL SCIENCE
(2023)
Article
Chemistry, Medicinal
Karoline B. Waitman, Larissa C. de Almeida, Marina C. Primi, Jorge A. E. G. Carlos, Claudia Ruiz, Thales Kronenberger, Stefan Laufer, Marcia Ines Goettert, Antti Poso, Sandra V. Vassiliades, Vinicius A. M. de Souza, Monica F. Z. J. Toledo, Neuza M. A. Hassimotto, Michael D. Cameron, Thomas D. Bannister, Leticia Costa-Lotufo, Joa o A. Machado-Neto, Mauricio T. Tavares, Roberto Parise-Filho
Summary: A series of hybrid inhibitors combining pharmacophores of known kinase inhibitors and benzohydroxamate HDAC inhibitors were synthesized and evaluated for their anticancer activity and pharmacokinetic properties. Compounds 4d-f exhibited promising cytotoxicity against hematological cells and moderate activity against solid tumor models. Compound 4d showed potent inhibition of multiple kinase targets and had stable interactions with HDAC and members of the JAK family. These compounds showed selective cytotoxicity with minimal effects on non-tumorigenic cells and favorable pharmacokinetic profiles.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2024)
Article
Biochemical Research Methods
Saranya Krishnan, Shina Sasi, Preshobha Kodakkattumannil, Salima Al Senaani, Geetha Lekshmi, Martin Kottackal, Khaled M. A. Amiri
Summary: This study aimed to develop an efficient DNA extraction protocol suitable for diverse plant species and tissues. A reliable and consistent protocol was described for the extraction of high-quality DNA from difficult-to-extract plant species. The optimized protocol was successful in extracting high-quality DNA from various plant species and tissues, making it useful for genomic studies of recalcitrant plants.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Eisaku Hokazono, Saori Fukumoto, Takeshi Uchiumi, Susumu Osawa
Summary: This study proposes a method for detecting nucleic acid amplification using pyrophosphate, which requires only two reagents and an automated analyzer. The technique has high sensitivity and reproducibility, and can detect pyrophosphate within 10 minutes. Therefore, this method has the potential to be a new, rapid, and simple detection technique for amplified nucleic acids.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Drew S. Sowersby, L. Kevin Lewis
Summary: SURE electrophoresis is a new method for concentrating samples in gels, which allows efficient detection of highly dilute DNA samples. This approach generates single bands with enhanced signal intensities and minimal band broadening.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Hongyi Liang, Guoliang Yin, Guangxi Shi, Zhiyong Liu, Xiaofei Liu, Jingwei Li
Summary: The mechanism of Echinacoside (ECH) in treating breast cancer (BC) was explored through network pharmacology and experimental validation. It was found that ECH plays an important role in anti-BC by regulating the PI3K/AKT/HIF-1 alpha/VEGF signaling pathway, and it exhibits multi-target and multi-pathway effects.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Daniel S. Kantner, Emily Megill, Anna Bostwick, Vicky Yang, Carmen Bekeova, Alexandria Van Scoyk, Erin L. Seifert, Michael W. Deininger, Nathaniel W. Snyder
Summary: This study compared the results of different methods for measuring the amount of acetyl-Coenzyme A. The colorimetric ELISA kit did not produce interpretable results, while the fluorometric enzymatic kit showed comparable results to the LC-MS-based methods depending on the matrix and extraction conditions. LC-MS/MS and LC-HRMS methods produced well-aligned results, especially when using stable isotope-labeled internal standards.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Jingyan Xu, Hanying Tan, Xionghui Ma, Linjing Su, Zhi Zhang, Yuhao Xiong
Summary: This study investigates the co-catalytic capabilities of MoO3 nanosheets in enhancing the enzyme-like catalytic activity of a two-dimensional ultrathin Fe(III)-modified covalent triazine framework (Fe-CTF) under neutral pH conditions. The Fe-CTF/MoO3 co-catalytic system exhibits enzyme-mimicking activity and enables the development of a colorimetric method for glucose detection. Furthermore, a straightforward one-pot colorimetric method is established for screening XOD inhibitors.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Lichao Zhang, Kang Xiao, Xueting Wang, Liang Kong
Summary: A novel fusion technology was designed to identify FAD-binding sites, achieving the best results on two independent test datasets and outperforming existing methods significantly. The high performance and certainty of the method were demonstrated through statistical tests and cross-entropy loss analysis.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Abdulkadir Kilic, Mehmet Aslan, Abdulkadir Levent
Summary: This study developed a simple, rapid, sensitive, and selective voltammetric technique for the electrochemical characterization and detection of the highly risky drug Edoxaban. The optimized voltammetric method showed good analytical working range and was successfully applied to urine and tablet samples.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Su Hyun Kim, Nara Shin, Jong-Min Jeon, Jeong-Jun Yoon, Jeong Chan Joo, Hee Taek Kim, Shashi Kant Bhatia, Yung-Hun Yang
Summary: To address environmental issues caused by traditional plastics, bioplastics have gained attention as alternatives. Although bioplastics have better degradability, their degradation still takes longer than anticipated. This study proposes a novel screening method to identify bioplastic degraders faster, saving time and providing more quantitative data.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Irem Okman Kocoglu, Pinar Esra Erden, Esma Kilic
Summary: In this study, an electrochemical biosensor based on carbon nanofibers and ionic liquid modification was constructed for tyramine detection. The biosensor showed linear response, low detection limit, high sensitivity, and exhibited good reproducibility, stability, and anti-interference ability.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Liana U. Akhmetzianova, Timur M. Davletkulov, Assol R. Sakhabutdinova, Alexey Chemeris, Irek M. Gubaydullin, Ravil R. Garafutdinov
Summary: A new program called LAMPrimers iQ has been developed for high-quality LAMP primer design, and its advantages in providing higher specificity and reliable detection of viral RNA were validated using SARS-CoV-2 coronavirus RNA as a model target.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Tommaso Pileri, Alberto Sinibaldi, Agostino Occhicone, Norbert Danz, Elena Giordani, Matteo Allegretti, Frank Sonntag, Peter Munzert, Patrizio Giacomini, Francesco Michelotti
Summary: This study developed a biosensing device based on one-dimensional photonic crystal to detect HER2 in breast cancer. The device combines label-free and fluorescence operation modes, allowing for real-time and accurate detection in less than 20 minutes. It offers a promising technique for combined label-free and fluorescence detection in disease diagnosis and therapeutic monitoring.
ANALYTICAL BIOCHEMISTRY
(2024)