Article
Chemistry, Multidisciplinary
Jia Miao, Minqian Miao, Yue Jiang, Min Zhao, Qing Li, Yuan Zhang, Yi An, Kanyi Pu, Qingqing Miao
Summary: A NIR-II fluorescent reporter with a donor-pi-acceptor architecture was developed for specific detection of A beta plaques in AD-model mice, enabling non-invasive imaging and deep deciphering of AD progression.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Biochemistry & Molecular Biology
Alexandra P. Orleneva, Vsevolod A. Serebrianyi, Ekaterina A. Kutukova, Tatiana A. Yampolskaya
Summary: The study identified the lacA gene in Talaromyces cellulolyticus and demonstrated its secretion and beta-galactosidase activity. The potential use of lacA as a reporter gene was also explored, showing its dependence on cultivation conditions similar to cellobiohydrolase activity.
MOLECULAR BIOTECHNOLOGY
(2022)
Article
Microbiology
Jessica N. Brandwein, Tiffany S. Sculthorpe, Miranda J. Ridder, Jeffrey L. Bose, Kelly C. Rice
Summary: This study provides new insight into the factors that regulate nos expression in Staphylococcus aureus, including SrrAB, Agr, Rex, and MgrA. The results demonstrate strain-dependent regulation of nos expression and a stronger expression during late-exponential low-oxygen growth.
MICROBIOLOGY SPECTRUM
(2023)
Article
Biotechnology & Applied Microbiology
Fuyun Hou, Taifeng Du, Zhen Qin, Tao Xu, Aixian Li, Shunxu Dong, Daifu Ma, Zongyun Li, Qingmei Wang, Liming Zhang
Summary: This study identified 17 beta-galactosidase genes in sweetpotato, which were classified into seven subfamilies. The promoter regions of these genes contained stress, hormone, and light responsive elements. Real-time PCR results showed distinct expression patterns of these genes across different tissues and varieties, suggesting their potential roles in plant development and stress responses.
Article
Microbiology
Anqi Chen, Danhui Wang, Sam R. Nugen, Juhong Chen
Summary: Utilizing an enzyme-based fluorescent assay, the study demonstrated a sensitive method for detecting the presence of E. coli in food samples, providing a straightforward approach to detect potential biological contamination. The engineered phages showed a detection limit of 10 CFU/mL and could be applied to detect E. coli strains from environmental samples.
Article
Chemistry, Multidisciplinary
Penghui Cheng, Shasha He, Chi Zhang, Jing Liu, Kanyi Pu
Summary: A tandem-locked NETosis reporter was developed for specific imaging of NETosis. This reporter can differentiate NETosis from neutrophil activation and provide prognosis for cancer immunotherapy. The study demonstrates a sensitive optical reporter for noninvasive monitoring of NETosis levels and evaluation of cancer immunotherapeutic efficacy.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Analytical
Ronglan Liu, Hongyu Chen, Shilu Zhang, Bo Zhao, Liang Zhou, Fan Zhang, Xufeng Nie, Jun Liu
Summary: Three fluorescent probes based on triarylboron (TAB) were designed and synthesized, and TAB-4-Gal was found to exhibit excellent performance, with fast response and strong fluorescence signal towards ovarian cancer cells. Therefore, it can serve as an ideal fluorescent probe for distinguishing ovarian cancer cells from other cells.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Biotechnology & Applied Microbiology
Erin E. Price, Paulami Rudra, Javiera Norambuena, Franklin Roman-Rodriguez, Jeffrey M. Boyd
Summary: Transcriptional reporters are essential tools for studying gene regulation, and in this study, new vectors were developed for markerless insertion of codon-optimized lacZ-based reporters in Staphylococcus aureus. These vectors allow tailored lacZ expression with different ribosomal binding sites. The deletion of crtM gene allows for blue-white screening without interference from carotenoid production, and the methods described are compatible for monitoring transcriptional activity in anaerobic cultures. The study provides important insights into gene regulation in low-oxygen environments for S. aureus, a key human pathogen.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Chemistry, Analytical
Shijun Chen, Xiaodong Ma, Haijie Wang, Lin Wang, Yuanyuan Wu, Yaping Wang, Yiyi Li, Wenkang Fan, Caoyuan Niu, Shicong Hou
Summary: Researchers have successfully developed a near-infrared fluorescence probe SN-CR for the visualization of the important biomarker beta-Gal in cell senescence and primary ovarian cancer. The probe has fast reaction equilibrium time, excellent biocompatibility, near-infrared emission, low detection limit, and no crosstalk between excitation and emission spectra.
ANALYTICA CHIMICA ACTA
(2023)
Article
Cell Biology
Li Hu, Chanjuan Dong, Zhe Wang, Shengyuan He, Yiwen Yang, Meiting Zi, Huiqin Li, Yanghuan Zhang, Chuanjie Chen, Runzi Zheng, Shuting Jia, Jing Liu, Xuan Zhang, Yonghan He
Summary: We developed a near-infrared fluorescent probe, XZ1208, that can be cleaved rapidly by beta-galactosidase and generates a strong fluorescence signal in senescent cells. XZ1208 showed high specificity and sensitivity in labeling senescent cells in various models. It achieved long-term duration in labeling senescence without causing significant toxicities.
Article
Chemistry, Analytical
Sungu Kim, Aparna Krishnamurthy, Pooja Kasiviswanathan, Baskar Ganapathysubramanian, Robbyn K. Anand
Summary: This study demonstrates the use of droplets and ion concentration polarization to rapidly lyse breast cancer cells and enrich low-abundance intracellular analytes.
ANALYTICAL CHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Shiv K. Sharma, Sijan Poudel Sharma, Roger M. Leblanc
Summary: The application of beta-galactosidase enzyme ranges from industrial use as probiotics to medically important application such as cancer detection. The irregular activities of beta-galactosidase enzyme are directly related to the development of cancers. Identifying the location and expression levels of enzymes in cancer cells have considerable importance in early-stage cancer diagnosis and monitoring the efficacy of therapies. Most importantly, the knowledge of the efficient method of detection of beta-galactosidase enzyme will help in the early-stage treatment of the disease. Further, we emphasized on the challenges and opportunities in this rapidly developing field of development of different biomarkers and fluorescent probes based on beta-galactosidase enzyme. We found that previously used chromo-fluorogenic methods have been mostly replaced by the new molecular probes, although they have certain drawbacks. Upon comparing the different methods, it was found that near-infrared fluorescent probes are dominating the other detection methods.
ENZYME AND MICROBIAL TECHNOLOGY
(2021)
Article
Chemistry, Analytical
Xiaowan Han, Xiangchuan Meng, Xiang Wang, Shan Leng, Qian Liu, Leilei Zhang, Pingping Li, Qingyang Zhang, Hai-Yu Hu
Summary: A new excited-state intramolecular proton transfer-delayed fluorescence (ESIPT-DF) approach was reported to construct analyte-stimulated DF probes. These new classes of ESIPT-DF luminophores, including HBT-PXZ and HBT-PTZ, exhibited significant ESIPT and DF characters. Furthermore, a caged probe HBT-PXZ-Ga was developed and applied to investigate the influence of metal ions on beta-gal activity.
ANALYTICAL CHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Natalia Klimova, Siwei Chu, Bernard Turcotte
Summary: Researcher created a synthetic reporter gene derived from the Vibrio cholerae lacZ gene to study gene expression in the human fungal pathogen Candida albicans. The synthetic lacZ reporter showed high beta-galactosidase activity, making it a useful tool for analyzing promoter activity in C. albicans.
FEMS YEAST RESEARCH
(2021)
Article
Chemistry, Analytical
Jie Mei, Jian Bao, Xia Cheng, Dandan Ren, Guanhong Xu, Fangdi Wei, Yong Sun, Qin Hu, Yao Cen
Summary: A novel dual-emissive SiNPs-Rho6G was synthesized using a hydrothermal method, offering strong fluorescence and photostability, ideal for establishing a ratiometric fluorescence sensor for beta-Galactosidase activity. This method enables high sensitivity and selectivity in determining enzyme activity, showing potential as an analysis platform for various target molecules.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Biochemical Research Methods
Saranya Krishnan, Shina Sasi, Preshobha Kodakkattumannil, Salima Al Senaani, Geetha Lekshmi, Martin Kottackal, Khaled M. A. Amiri
Summary: This study aimed to develop an efficient DNA extraction protocol suitable for diverse plant species and tissues. A reliable and consistent protocol was described for the extraction of high-quality DNA from difficult-to-extract plant species. The optimized protocol was successful in extracting high-quality DNA from various plant species and tissues, making it useful for genomic studies of recalcitrant plants.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Eisaku Hokazono, Saori Fukumoto, Takeshi Uchiumi, Susumu Osawa
Summary: This study proposes a method for detecting nucleic acid amplification using pyrophosphate, which requires only two reagents and an automated analyzer. The technique has high sensitivity and reproducibility, and can detect pyrophosphate within 10 minutes. Therefore, this method has the potential to be a new, rapid, and simple detection technique for amplified nucleic acids.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Drew S. Sowersby, L. Kevin Lewis
Summary: SURE electrophoresis is a new method for concentrating samples in gels, which allows efficient detection of highly dilute DNA samples. This approach generates single bands with enhanced signal intensities and minimal band broadening.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Hongyi Liang, Guoliang Yin, Guangxi Shi, Zhiyong Liu, Xiaofei Liu, Jingwei Li
Summary: The mechanism of Echinacoside (ECH) in treating breast cancer (BC) was explored through network pharmacology and experimental validation. It was found that ECH plays an important role in anti-BC by regulating the PI3K/AKT/HIF-1 alpha/VEGF signaling pathway, and it exhibits multi-target and multi-pathway effects.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Daniel S. Kantner, Emily Megill, Anna Bostwick, Vicky Yang, Carmen Bekeova, Alexandria Van Scoyk, Erin L. Seifert, Michael W. Deininger, Nathaniel W. Snyder
Summary: This study compared the results of different methods for measuring the amount of acetyl-Coenzyme A. The colorimetric ELISA kit did not produce interpretable results, while the fluorometric enzymatic kit showed comparable results to the LC-MS-based methods depending on the matrix and extraction conditions. LC-MS/MS and LC-HRMS methods produced well-aligned results, especially when using stable isotope-labeled internal standards.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Jingyan Xu, Hanying Tan, Xionghui Ma, Linjing Su, Zhi Zhang, Yuhao Xiong
Summary: This study investigates the co-catalytic capabilities of MoO3 nanosheets in enhancing the enzyme-like catalytic activity of a two-dimensional ultrathin Fe(III)-modified covalent triazine framework (Fe-CTF) under neutral pH conditions. The Fe-CTF/MoO3 co-catalytic system exhibits enzyme-mimicking activity and enables the development of a colorimetric method for glucose detection. Furthermore, a straightforward one-pot colorimetric method is established for screening XOD inhibitors.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Lichao Zhang, Kang Xiao, Xueting Wang, Liang Kong
Summary: A novel fusion technology was designed to identify FAD-binding sites, achieving the best results on two independent test datasets and outperforming existing methods significantly. The high performance and certainty of the method were demonstrated through statistical tests and cross-entropy loss analysis.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Abdulkadir Kilic, Mehmet Aslan, Abdulkadir Levent
Summary: This study developed a simple, rapid, sensitive, and selective voltammetric technique for the electrochemical characterization and detection of the highly risky drug Edoxaban. The optimized voltammetric method showed good analytical working range and was successfully applied to urine and tablet samples.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Su Hyun Kim, Nara Shin, Jong-Min Jeon, Jeong-Jun Yoon, Jeong Chan Joo, Hee Taek Kim, Shashi Kant Bhatia, Yung-Hun Yang
Summary: To address environmental issues caused by traditional plastics, bioplastics have gained attention as alternatives. Although bioplastics have better degradability, their degradation still takes longer than anticipated. This study proposes a novel screening method to identify bioplastic degraders faster, saving time and providing more quantitative data.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Irem Okman Kocoglu, Pinar Esra Erden, Esma Kilic
Summary: In this study, an electrochemical biosensor based on carbon nanofibers and ionic liquid modification was constructed for tyramine detection. The biosensor showed linear response, low detection limit, high sensitivity, and exhibited good reproducibility, stability, and anti-interference ability.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Liana U. Akhmetzianova, Timur M. Davletkulov, Assol R. Sakhabutdinova, Alexey Chemeris, Irek M. Gubaydullin, Ravil R. Garafutdinov
Summary: A new program called LAMPrimers iQ has been developed for high-quality LAMP primer design, and its advantages in providing higher specificity and reliable detection of viral RNA were validated using SARS-CoV-2 coronavirus RNA as a model target.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Tommaso Pileri, Alberto Sinibaldi, Agostino Occhicone, Norbert Danz, Elena Giordani, Matteo Allegretti, Frank Sonntag, Peter Munzert, Patrizio Giacomini, Francesco Michelotti
Summary: This study developed a biosensing device based on one-dimensional photonic crystal to detect HER2 in breast cancer. The device combines label-free and fluorescence operation modes, allowing for real-time and accurate detection in less than 20 minutes. It offers a promising technique for combined label-free and fluorescence detection in disease diagnosis and therapeutic monitoring.
ANALYTICAL BIOCHEMISTRY
(2024)