Article
Chemistry, Multidisciplinary
Si Si Liew, Ziling Zeng, Penghui Cheng, Shasha He, Chi Zhang, Kanyi Pu
Summary: This study presents a near-infrared fluorescence activatable molecular probe for detecting SARS-CoV-2 protease in living mice, enabling sensitive detection of viral protease and optical urinalysis of viral infection. This in vivo sensor holds potential for preclinical high-throughput drug screening and clinical diagnostics in respiratory viral infections.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Review
Chemistry, Applied
Songjiao Li, Peipei Wang, Ke Yang, Ying Liu, Dan Cheng, Longwei He
Summary: A near-infrared methylene blue-based fluorogenic probe with on/off activation ability is crucial for detecting and monitoring biological analytes within cells and organisms. By using biomarker-activated probes, precise results can be obtained with minimal photodamage and biological interferences. This review highlights the recent advancements in activatable methylene blue-based fluorogenic probes, focusing on strategies for detecting and imaging disease-related biomarkers and the potential challenges and opportunities in developing new methods to detect and image biomarkers.
Article
Chemistry, Analytical
Ning Ding, Zhao Li, Yitong Hao, Chengxiao Zhang
Summary: A novel near-infrared fluorescent probe was designed for the visualization and detection of formaldehyde in biologic organisms. The probe showed high sensitivity, selectivity, and fast reaction time, providing a new theoretical basis for rapid and sensitive formaldehyde detection and in vivo imaging applications.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Wenjing Wu, Xing Li, Lingyu Zhao, Shumu Li, Juanjuan Han, Yangyang Zhang, Zhenwen Zhao
Summary: We report a novel two-photon fluorescent probe for the detection of NQO1 that demonstrates superior analytical performance with a large Stokes shift and deep tissue penetration.
CHEMICAL COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Oshin D. Warerkar, Niyati H. Mudliar, Tanya Ahuja, Sailee D. Shahane, Prabhat K. Singh
Summary: The development of an accurate and sensitive sensor for detecting amyloid plaques is crucial for early diagnosis of protein disorders like Alzheimer's disease. The use of hemicyanine-based probe LDS730, belonging to the Near-Infrared Fluorescence family, provides higher precision, prevents photo-damage, and minimizes autofluorescence. The sensor exhibits a 110-fold increase in fluorescence turn-on emission when bound to insulin fibrils, making it highly sensitive with great potential for early detection and improving diagnostic accuracy.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Review
Chemistry, Inorganic & Nuclear
Tao Jia, Guanying Chen
Summary: Fluorescence imaging in the second near-infrared biological window offers unique advantages and lanthanide-based nanoparticles as NIR-II probes have attracted extensive attention. Recent advances have enabled lanthanide-based nanoparticles to exhibit well-defined luminescence profiles in the NIR-II region.
COORDINATION CHEMISTRY REVIEWS
(2022)
Article
Spectroscopy
Chang Liu, Huimin Yan, Jianhong Wu, Zhiming Wang, Song He, Liancheng Zhao, Xianshun Zeng
Summary: A new near-infrared fluorescent probe Qcy-OH based on an acceptor-donor-acceptor (ADA) pi-electron system with a turn-on mechanism was developed for monitoring pH fluctuations in biological systems. The probe showed a strong pH-sensitive response and high selectivity for intracellular lysosomes, enabling the tracking of lysosomal pH changes.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Review
Biochemistry & Molecular Biology
Zhipeng Zhang, Peiyao Chen, Yao Sun
Summary: Near-infrared (NIR) fluorescence is a promising imaging technique that is noninvasive, highly sensitive, and high-resolution. Enzymes are ideal triggers for activating NIR fluorescent probes, which have better signal-to-noise ratio compared to Always-On probes. This review provides an overview of enzyme-activatable NIR fluorescent probes, focusing on design strategies, sensing mechanisms, and current challenges in construction.
Article
Chemistry, Analytical
Chu Tang, Hongjuan Tong, Bin Liu, Xinan Wang, Yushen Jin, Erli Tian, Fu Wang
Summary: In this study, a novel estrogen receptor alpha (ER α)-targeted near-infrared fluorescence probe was developed for rapid and sensitive detection of hydrazine levels. The probe exhibited good ER α affinity, excellent selectivity, and high biocompatibility. It can visualize hydrazine concentration rapidly and provide high contrast in vivo imaging.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Applied
Jiahou Geng, Juan Zheng, Wenjun Bai, Hui Yang, Yang Lu, Ning Wang, Yufen Zhao, Jinhui Wang
Summary: A near-infrared fluorescence probe PTP was developed to visualize the distribution of nitroreductase (NTR) in tumor cells, demonstrating a fast response and excellent biocompatibility.
Article
Biochemistry & Molecular Biology
Shuting Wang, Shulei Zhu, Yawen Tanzeng, Yuexing Zhang, Chuchu Li, Mingliang Ma, Wei Lu
Summary: A series of novel near-infrared fluorescent molecules were designed with a compact donor-acceptor-donor architecture, exhibiting significant variations in fluorescence intensity and viscosity sensitivity in different solvent polarities. Among them, one molecule M-3 showed a 3-fold increase in emission intensity in a high viscosity solvent system and a 4-fold increase in fluorescence intensity in the presence of proteins, demonstrating superior environmental sensitivity and potential for optical imaging probe development.
Article
Chemistry, Analytical
Liqiang Yan, Hong Yang, Jia Li, Cuiping Zhou, Lin Li, Xiongzhi Wu, Chenghong Lei
Summary: The fluorescent probe prepared in this study can rapidly and effectively detect zinc ions and hypochloric acid, showing good optical properties for cell imaging and satisfactory recoveries in actual water samples.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Analytical
Jianlong Ma, Xiaofeng Lu, Yong Guo, Zhijun Wang
Summary: This study synthesized a near-infrared (NIR) Cys-special probe (MCyA) that could precisely and quickly detect Cys activity. The observations showed that the endogenous Cys activity was depressed under oxidative stress, but dramatically increased with omeprazole stimulation in HepG2 cells. MCyA was also successfully used to image the upregulation of Cys level in HepG2 cells in the presence of methionine (Met). Overall, MCyA is a novel and practical tool to study Cys-related physiological and pathological processes.
Article
Chemistry, Applied
Meng-Jiao Gao, Yun Hua, Jia-Qi Xu, Li-Xia Zhang, Shuo Wang, Yan-Fei Kang
Summary: In this study, a large stokes shift and near-infrared probe DCM-HNU was designed and synthesized to visualize H2O2 in living cells and zebra fish, showing great potential for exploring the function and participation of H2O2 in biological systems.
Article
Chemistry, Analytical
Zongcheng Wang, Shun Yang, Xiuqi Liu, Tingting Yang, Ting Han, Xingrui He, Yuren Jiang, Yuanqiang Hao
Summary: The newly designed fluorescent probe YZ-M5 shows high accuracy and sensitivity in detecting and visualizing selenocysteine, with rapid and strong fluorescence enhancement within 5 minutes. It has a low detection limit and a wide detection range, as well as the ability to identify selenocysteine in living cells and mice.
MICROCHEMICAL JOURNAL
(2021)
Article
Chemistry, Multidisciplinary
Xinzhu Wang, Shasha He, Penghui Cheng, Kanyi Pu
Summary: A dual-locked and tandem activatable probe (DTAP) is developed for real-time NIRF imaging of intratumoral pyroptosis during cancer chemo-immunotherapy in living mice. The activated signal of DTAP correlates well with tumor-infiltrating cytotoxic T lymphocytes and tumor growth inhibition, enabling the prediction of cancer therapeutic efficacy. This study provides a non-invasive technique to study the regulatory mechanism of pyroptosis in cancer therapy and optimize cancer chemo-immunotherapies.
ADVANCED MATERIALS
(2023)
Article
Engineering, Biomedical
Cheng Xu, Jingsheng Huang, Yuyan Jiang, Shasha He, Chi Zhang, Kanyi Pu
Summary: Molecular imaging via afterglow luminescence is improved by using organic nanoparticles that produce ultrasound-induced afterglow. These sonoafterglow nanoparticles have higher brightness and detection capabilities at larger tissue depths compared to light-induced afterglow. They were successfully applied in cancer immunotheranostics, allowing for real-time in vivo monitoring of physiopathological processes.
NATURE BIOMEDICAL ENGINEERING
(2023)
Article
Chemistry, Multidisciplinary
Mengke Xu, Chi Zhang, Shasha He, Cheng Xu, Xin Wei, Kanyi Pu
Summary: Photothermal immunotherapy is a combination cancer treatment that uses photothermal processes to destroy cancer cells and stimulate antitumor immunity. However, cancer cells can resist this treatment by deactivating immunoproteases. This study presents a smart polymer nanoagonist that can release an inhibitor of serpins, allowing for enhanced cancer immunotherapy. Additionally, the nanoagonist induces photothermal therapy for tumor ablation and immunogenic cancer cell death.
Article
Chemistry, Multidisciplinary
Xiang Wu, Fan Yang, Sa Cai, Kanyi Pu, Guosong Hong
Summary: The NIR-II window, ranging from 1000 to 1700 nm in wavelength, allows for deep-penetration fluorescent imaging in biological tissues due to reduced light scattering. Recently, deep-brain neuromodulation has been achieved in the NIR-II window using nanotransducers that convert NIR-II light into heat. This Perspective discusses the principles, potential applications, advantages, and limitations of this NIR-II deep-brain neuromodulation technique, as well as future directions for advancement.
Review
Chemistry, Multidisciplinary
Chi Zhang, Kanyi Pu
Summary: This review discusses the convergence of sonodynamic therapy (SDT) with cancer immunotherapy to overcome their limitations for clinical translation. The focus is on the development of organic materials with high sonodynamic activity and immunotherapeutic efficiency. SDT induces immunogenic cell death to improve tumor immunogenicity, and immunotherapy drugs activate antitumor immunity through immune pathway modulation.
ADVANCED MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Cheng Xu, Shasha He, Xin Wei, Jingsheng Huang, Mengke Xu, Kanyi Pu
Summary: In this study, a ultrasound-induced afterglow (sonoafterglow) nanoprobe, named Q-SNAP, was developed for real-time imaging of T-cell immunoactivation. The probe specifically detects granzyme B and has a low limit of detection. Q-SNAP can not only distinguish autoimmune hepatitis from healthy liver at an early stage, but also monitor the reversal of T-cell hyperactivation. It offers the possibilities of dynamic monitoring of T-cell dysfunction and evaluation of immunotherapy in deep-seated lesions.
ADVANCED MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Jingchao Li, Ningyue Yu, Dong Cui, Jiaguo Huang, Yu Luo, Kanyi Pu
Summary: This study presents the construction of semiconducting polymer pro-nanomodulators (SPpMs) for deep-tissue sono-immunotherapy of orthotopic pancreatic cancer. Through ultrasound treatment, SPpMs can generate singlet oxygen molecules to ablate tumors and release immunomodulators, enhancing antitumor immune response.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Multidisciplinary
Jie Yu, Shasha He, Chi Zhang, Cheng Xu, Jingsheng Huang, Mengke Xu, Kanyi Pu
Summary: We report the development of polymeric STING pro-agonists (PSPA) for cancer sono-immunotherapy. PSPA is activated by sono-irradiation and elevated GSH within the tumor microenvironment (TME). Under sono-irradiation, PSPA generates O-1(2) and induces immunogenic cell death (ICD) of malignant tumor cells. Furthermore, the release of STING agonist (MSA-2) is specific to the tumor microenvironment with highly expressed GSH, minimizing off-target side effects. The activation of the STING pathway synergizes with SDT to enhance the anti-tumor response.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Multidisciplinary
Penghui Cheng, Rending Wang, Shasha He, Pengpeng Yan, Hongfeng Huang, Jianghua Chen, Jia Shen, Kanyi Pu
Summary: We report the development of Artificial bioMarker Probes (AMPros) for sensitive urinalysis of Acute renal allograft rejection (ARAR) in murine models. AMPros specifically react with immune biomarkers in the kidneys and activate their fluorescence signals to detect rejection. The urinalysis using AMPros is noninvasive, sensitive, and can detect ARAR earlier than current diagnostic methods.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Multidisciplinary
Shasha He, Jie Yu, Mengke Xu, Chi Zhang, Cheng Xu, Penghui Cheng, Kanyi Pu
Summary: This article reports on a novel therapy for ultra-sensitive sono-metallo-immunotherapy, which can eliminate tumor cells and regulate the tumor immune microenvironment, potentially leading to deep tumor inhibition.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)