Article
Chemistry, Multidisciplinary
Ouyang Dan, Chuanzhe Wang, Chao Zhong, Juan Lin, Gang Xu, Guane Wang, Zian Lin
Summary: In this study, novel LDI-MS substrates were designed to enhance the sensitivity of mass signals and achieve high accuracy diagnosis of different saccharides and central precocious puberty (CPP).
Review
Biochemical Research Methods
Lanaia Itala Louzeiro Maciel, Ricardo Alves Bernardo, Rafael Oliveira Martins, Almir Custodio Batista Jr, Joao Victor Ataide Oliveira, Andrea Rodrigues Chaves, Boniek Gontijo Vaz
Summary: The imaging of biological tissues using mass spectrometry imaging (MSI) techniques allows for the visualization of the distribution of various metabolites, drugs, lipids, and glycans in complex samples. MSI methods like DESI-MSI and MALDI-MSI provide high sensitivity and the ability to evaluate/visualize multiple analytes in a single sample, overcoming limitations of classical microscopy techniques. This review discusses the application of DESI-MSI and MALDI-MSI in evaluating exogenous and endogenous molecules in biological samples, providing technical insights and recent research findings.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Hua Zhang, Lin Zhao, Jingjing Jiang, Jie Zheng, Li Yang, Yanyan Li, Jian Zhou, Tianshu Liu, Jianmin Xu, Wenhui Lou, Weige Yang, Lijie Tan, Weiren Liu, Yiyi Yu, Meiling Ji, Yaolin Xu, Yan Lu, Xiaomu Li, Zhen Liu, Rong Tian, Cheng Hu, Shumang Zhang, Qinsheng Hu, Yangdong Deng, Hao Ying, Sheng Zhong, Xingdong Zhang, Yunbing Wang, Hua Wang, Jingwei Bai, Xiaoying Li, Xiangfeng Duan
Summary: As cancer is increasingly seen as a metabolic disorder, serum metabolite profiling is proposed as a viable method for detecting cancer. In this study, a machine learning model using mass spectrometry-based liquid biopsy data is reported for pan-cancer screening and classification. The Multiplexed Nanomaterial-Assisted LDI for Cancer Identification (MNALCI) achieves high sensitivity and specificity for distinguishing cancers from healthy controls and accurately identifying the tissue of origin of tumors. This high throughput assay shows promising potential for non-invasive cancer diagnosis.
NATURE COMMUNICATIONS
(2022)
Article
Plant Sciences
Yukun Liu, Xiaofei Nie, Jilong Wang, Zhenqi Zhao, Zhimei Wang, Fang Ju
Summary: Flavonoids in litchi seeds have broad-spectrum antiviral and antitumor activities, especially against breast and liver cancers. MALDI-MSI was used to detect and image the distribution of flavonoids in litchi seed tissue sections for the first time, revealing their heterogeneous distribution and potential for extraction.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Chemistry, Analytical
Frederic Fournelle, Nidia Lauzon, Ethan Yang, Pierre Chaurand
Summary: Traditionally, MALDI IMS requires the deposition of an organic matrix, which often generates high background noise in the low mass range. LDI offers selective ionization with low background in the low mass range. This article discusses the advantages and disadvantages of solvent free and wet deposition of metals as LDI matrices and demonstrates the power of metal-assisted LDI for analyzing low molecular weight molecules in tissue sections.
MICROCHEMICAL JOURNAL
(2023)
Article
Food Science & Technology
Qi Wang, Xiaohui Li, Hongping Wang, Simeng Li, Chen Zhang, Xueying Chen, Jing Dong, Hua Shao, Jing Wang, Fen Jin
Summary: A matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) method was developed to obtain dynamic images of forchlorfenuron in oriental melons. The majority of forchlorfenuron was found in the exocarp and mesocarp regions of the melons and decreased within two days after application. The degradation rate obtained by MALDI-MSI was reliable and comparable to HPLC-MS/MS, indicating its practicability for pesticide degradation study.
Article
Biochemistry & Molecular Biology
Chi-Sheng Chen, Wen-Chi Chen, Sarah Y. Chang
Summary: A new mode of electromembrane extraction (EME) has been developed for detection via matrix-assisted laser desorption/ionization mass spectrometry (MALDI/MS). The method successfully extracted and analyzed posaconazole, and was applied to human serum samples.
Article
Chemistry, Applied
Suying Li, Tsz-Tsun Ng, Zhong-Ping Yao
Summary: This study developed a new method using MALDI-MS and PLS-R to quantitate triacylglycerols in blended oils, allowing simultaneous quantitation of multiple compositions with good limits of detection. The new method enables direct analysis of blended oils, rapid analysis of samples, and accurate quantitation of low-abundance oil compositions and blended oils with similar fatty acid contents, compared to conventional methods.
Article
Nanoscience & Nanotechnology
Peiqi Luo, Liping Wang, Lixia Jiang, Jie Sun, Yafeng Li, Huihui Liu, Caiqiao Xiong, Zongxiu Nie
Summary: Graphdiyne, a new carbon nanomaterial, has been utilized as a matrix in laser desorption ionization mass spectrometry for small molecule analysis, showing enhanced desorption and ionization efficiency. The use of Graphdiyne in LDI MS analysis can potentially improve the early diagnosis of liver cancer by identifying fatty acids as potential biomarkers.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Microbiology
Anil K. Persad, Hanan A. Fahmy, Nicholas Anderson, Jing Cui, Zeynal Topalcengiz, Saharuetai Jeamsripong, Patrick M. Spanninger, Robert L. Buchanan, Kalmia E. Kniel, Michele T. Jay-Russell, Michelle D. Danyluk, Gireesh Rajashekara, Jeffrey T. LeJeune
Summary: This study demonstrates that MALDI-TOF is a viable alternative for the rapid identification and differentiation of Salmonella serovars.
Review
Spectroscopy
Wendy H. Mueller, Alexandre Verdin, Edwin De Pauw, Cedric Malherbe, Gauthier Eppe
Summary: Surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) is a technique that uses nanostructured substrates to promote the desorption/ionization of analytes, allowing for both untargeted and targeted analysis. This review focuses on SALDI-MS imaging (SALDI-MSI), discussing the nomenclature, fundamental mechanisms, analytical strategies, and applications in various fields. SALDI-MSI has strengths and limitations, but shows promising prospects for future development.
MASS SPECTROMETRY REVIEWS
(2022)
Article
Multidisciplinary Sciences
Gabriel R. Martins, Felipe L. Brum, Davi Marconi Miranda Carvalho da Silva, Livia C. Barbosa, Ronaldo Mohana-Borges, Ayla Sant'Ana da Silva
Summary: Matrix-assisted laser desorption/ionization-imaging mass spectrometry (MALDI-IMS) is a valuable technique for identifying compounds and understanding their physiological information in plant tissues. Researchers have developed a sample preparation protocol to obtain thin sections of hard seeds, enabling molecular mapping by MALDI-IMS. This method has the potential to be a valuable tool for studying the morphology, anatomy, and physiology of botanical materials.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2023)
Article
Chemistry, Analytical
Xian-Na Wang, Bin Li
Summary: Gold nanoparticles/thiol-beta-cyclodextrin-functionalized TiO2 nanowires were prepared to enhance the performance of SALDI MS and MSI, showing superior detection sensitivity and repeatability.
ANALYTICAL CHEMISTRY
(2022)
Review
Chemistry, Physical
Wen Ma, Jun Li, Xianjiang Li, Yu Bai, Huwei Liu
Summary: The rapid advancement of nanotechnology in the field of SALDI-MS has led to the development of novel nanoparticles and nanostructured substrates, improving detection sensitivity and specificity, and impacting the field of biomedical analysis significantly.
Review
Chemistry, Multidisciplinary
Yajie Ding, Congcong Pei, Weikang Shu, Jingjing Wan
Summary: Metabolic analysis in biofluids plays a crucial role in disease diagnosis and monitoring, and LDI MS-based metabolic analysis shows great potential in this field. This review discusses the essential elements of LDI MS-based metabolic analysis and summarizes the recent developments in diagnostic applications using different nanomaterials and machine learning algorithms.
CHEMISTRY-AN ASIAN JOURNAL
(2022)
Editorial Material
Biochemistry & Molecular Biology
Cosima Damiana Calvano, Ilario Losito, Tommaso Cataldi
Summary: This special issue focuses on the contribution of dysregulation of lipid signaling and metabolism to neurodegenerative diseases, including Alzheimer's and Parkinson's, summarizing three reviews and one research article.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Agriculture, Multidisciplinary
Andrea Castellaneta, Ilario Losito, Beniamino Leoni, Pietro Santamaria, Cosima Damiana Calvano, Tommaso R. Cataldi
Summary: Microgreens are a special type of vegetal product with potentially high content of various nutrients, including glycerophospholipids. The study found that glycerophospholipids in different microgreens are rich in essential fatty acids, which can serve as additional sources in vegetarian/vegan diets.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Review
Spectroscopy
Raffaella Pascale, Maria A. Acquavia, Alberto Onzo, Tommaso R. I. Cataldi, Cosima D. Calvano, Giuliana Bianco
Summary: Surfactants are widely used in personal care products, detergents, and industrial applications, requiring sensitive analytical methods for determining their low concentration levels in complex matrices. The introduction of mass spectrometry techniques has significantly expanded research in the field, allowing for the analysis of complex mixtures and degradation products of surfactants at trace levels.
MASS SPECTROMETRY REVIEWS
(2021)
Article
Biochemical Research Methods
Davide Coniglio, Giovanni Ventura, Cosima D. Calvano, Ilario Losito, Tommaso R. Cataldi
Summary: This study presents a new method to determine the carbon-carbon double bond positions of fatty acyl chains in water-soluble diacyl arsenosugar phospholipids (As-PL). Using this method, abundant unsaturated fatty acids were found in the brown alga wakame, confirming it as a good source of essential fatty acids with a balanced omega 6/omega 3 ratio.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2022)
Article
Biochemistry & Molecular Biology
Antonio Monopoli, Giovanni Ventura, Andrea Aloia, Fulvio Ciriaco, Angelo Nacci, Tommaso R. Cataldi, Cosima D. Calvano
Summary: This study explored the rational design of matrix for enhanced sensitivity and overall performance of matrix-assisted laser desorption/ionization mass spectrometry. Various matrices with different functionalities and substituents were synthesized and tested across different sample types. The results demonstrated improved performance in terms of ionization capability and interference-free spectra, especially for the analysis of phospholipids and neutral lipids.
Article
Chemistry, Analytical
Mariachiara Bianco, Giovanni Ventura, Cosima Damiana Calvano, Ilario Losito, Tommaso R. Cataldi
Summary: With the introduction of novel nutrient sources with high protein content in the consumer market, safety evaluation studies on potentially allergenic proteins are necessary. This study presents a method for establishing sequence-based homology between proteins of spirulina and chlorella micro-algae and known allergens through database searches. Several potentially allergenic proteins were identified, and further immunochemical tests are required to confirm their allergenicity.
Editorial Material
Food Science & Technology
Cosima D. Calvano, Antonia Tamborrino
Article
Biochemical Research Methods
Andrea Castellaneta, Ilario Losito, Giovanni Cisternino, Beniamino Leoni, Pietro Santamaria, Cosima Damiana Calvano, Giuliana Bianco, Tommaso R. I. Cataldi
Summary: An analytical approach based on RPLC-ESI-(-)-FTMS was developed for the untargeted characterization of glucosinolates in the polar extracts of four Brassica microgreen crops. The method involved the systematic fragmentation of all ions generated in the electrospray source, followed by the acquisition of an FTMS spectrum. The identification of GSL was achieved by recognizing the best qualifying product ions and aligning the peaks detected in the EIC traces.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2022)
Article
Biochemistry & Molecular Biology
Nicola Giangregorio, Annamaria Tonazzi, Cosima Damiana Calvano, Ciro Leonardo Pierri, Giovanna Incampo, Tommaso R. I. Cataldi, Cesare Indiveri
Summary: The effect of mycotoxin patulin on the mitochondrial carnitine/acylcarnitine carrier was investigated. Patulin inhibited the transport function of the carrier in a competitive manner, interacting with a protein region including the substrate-binding area. Site-directed mutagenesis confirmed the key role of the C136 residue in the inhibition of patulin.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Marco Bottin, Graziano Di Carmine, Olga Bortolini, Carmela De Risi, Monica Bertoldo, Tommaso R. I. Cataldi, Cosima D. Calvano, Alessandro Massi, Daniele Ragno
Summary: This article presents an alternative synthetic route to macrocyclic oligoesters (MCOs) using N-heterocyclic carbene (NHC) catalysis under highly diluted oxidative conditions. The method allows for the straightforward access to MCOs using commercial dialdehydes, without the use of toxic diacyl chlorides commonly employed in traditional processes. The method is metal-free, takes place in a green solvent, and uses a recyclable quinone oxidant.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Ramona Abbattista, Ilario Losito, Graziana Basile, Andrea Castellaneta, Giovanni Ventura, Cosima Damiana Calvano, Tommaso R. I. Cataldi
Summary: Reversed-phase liquid chromatography and electrospray ionization with Fourier-transform single and tandem mass spectrometry were used to characterize the structure of oleocanthal (OLEO) and oleacin (OLEA), two important bioactive compounds in extra virgin olive oil. The presence of multiple isoforms of OLEO and OLEA was observed, along with minor peaks of oxidized OLEO. The correlation between chromatographic peaks and specific isoforms was elucidated through H/D exchange experiments, revealing the prevalence of Open Forms II as the main isoforms.
Article
Materials Science, Multidisciplinary
Elena C. L. Rigante, Cosima D. Calvano, Rosaria A. Picca, Francesca Modugno, Tommaso R. I. Cataldi
Summary: In recent decades, modern murals and graffiti have gained attention as valuable artistic expressions that need to be preserved. Understanding the chemical composition of spray paints used in these artworks is crucial for their conservation. This study used various analytical techniques to investigate the composition of two yellow varnishes, revealing the use of acrylic and styrene-acrylic resins as binders, as well as the presence of silicone-based additives.
Article
Chemistry, Physical
Valentina Petrelli, Giuseppe Romanazzi, Cecilia Mortalo, Cristina Leonelli, Mauro Zapparoli, Elvira De Giglio, Cosima Damiana Calvano, Maria Michela Dell'Anna, Piero Mastrorilli
Summary: A nitrogen-doped polymer supported cobalt catalyst (Co-pol) was synthesized and used as an active and recyclable catalyst for nitroarene reduction. The catalysis was truly heterogeneous and prevented hydrodehalogenation. STEM and EDS analyses confirmed the presence of cobalt(0) nanoparticles as the active species. Nitrogen atoms in the catalytic support played a beneficial role in the catalytic activity.
MOLECULAR CATALYSIS
(2023)
Article
Biochemistry & Molecular Biology
Giovanni Ventura, Davide Mesto, Davide Blasi, Tommaso R. I. Cataldi, Cosima Damiana Calvano
Summary: This study investigated the differences in the ethanolic extract of dried walnut shells obtained through hammer milling and ball milling grinding processes. The study analyzed the fatty acids and phenolic compounds in the extracts and found that the composition varied depending on the milling method used. The presence of potential active secondary metabolites was also explored, providing valuable information for potential applications in industries such as biorefinery and pharmaceuticals.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Horticulture
Onofrio Davide Palmitessa, Andrea Castellaneta, Annalisa Somma, Adriano Didonna, Massimiliano Renna, Ilario Losito, Cosima Damiana Calvano, Tommaso R. I. Cataldi, Pietro Santamaria
Summary: Scopatizzo, a local variety of melon from Southern Italy, is consumed as an unripe melon alternative to cucumber due to its better quality profile and lack of cucurbitacins. However, recent analysis has found the presence of cucurbitacins in some bitter-tasting Scopatizzo fruits, raising concerns about potential genotypes not suitable for commercialization.