Article
Chemistry, Physical
Umit Celik, Kuebra Celik, Ihsan Kehribar, Suleyman Celik, Mehmet Z. Baykara
Summary: Forming a fundamental understanding of tribological processes on the atomic scale has the potential to revolutionize the control of friction and wear in macroscopic mechanical systems. However, current methods for studying tribology at the nanoscale, such as atomic force microscopy, are limited in scanning speed compared to macroscopic mechanical processes. In this study, a new method combining atomic force microscopy with quartz crystal microbalance measurements is proposed for high-speed nanoscale tribology.
APPLIED SURFACE SCIENCE
(2023)
Article
Chemistry, Physical
L. Bar, P. Losada-Perez, J. Troncoso
Summary: The influence of peptides on the stability of lipid membranes was studied using diphenylalanine and 1,2-dimyristoyl-sn-glycero-3-phosphocholine as model systems. Different experimental techniques were used to prepare and characterize vesicles at different peptide-lipid ratios. The results showed that diphenylalanine decreased lipid molecular cooperativity and weakened membrane organization.
JOURNAL OF MOLECULAR LIQUIDS
(2023)
Article
Chemistry, Multidisciplinary
Laure Bar, George Cordoyiannis, Shova Neupane, Jonathan Goole, Patrick Grosfils, Patricia Losada-Perez
Summary: The interest in nano-sized lipid vesicles in nano-biotechnology lies in their use as mimics for endosomes, exosomes, and nanocarriers for drug delivery. The transfer kinetics of lipid at minimal concentrations can be monitored using label-free surface-sensitive method of quartz crystal microbalance with dissipation monitoring.
Article
Biophysics
L. Bar, F. Perissinotto, L. Redondo-Morata, M. Giannotti, J. Goole, P. Losada-Perez
Summary: This study investigates the effect of topographical homogeneity of supported lipid bilayers (SLBs) on the adsorption of hydrophilic Quantum dots (QDs). It reveals significant differences in the interactions of QDs onto homogeneous and inhomogeneous SLBs, with QDs preferentially targeting membrane defects in inhomogeneous layers. The adsorption of QDs onto homogeneous SLBs is mainly driven by electrostatic interactions, while in inhomogeneous SLBs it is influenced by a subtle interplay of electrostatic and entropic effects.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2022)
Article
Materials Science, Multidisciplinary
Shova Neupane, Kai Betlem, Frank Uwe Renner, Patricia Losada-Perez
Summary: Solvent-assisted lipid bilayer (SALB) formation is a versatile approach for creating supported lipid membranes (SLBs) on metal surfaces, with lipid concentration in the solvent being a key parameter. The study found that at low concentrations, mostly monolayers are present, while at high concentrations, diverse structures coexist. The mechanism of SALB formation leads to unique two-step features in approach force curves on SLBs.
PHYSICA STATUS SOLIDI A-APPLICATIONS AND MATERIALS SCIENCE
(2021)
Article
Materials Science, Paper & Wood
Evdokia K. Oikonomou, Grazia M. L. Messina, Laurent Heux, Giovanni Marletta, Jean-Francois Berret
Summary: Concentrated fabric conditioners with cationic surfactants make fibers softer, and the addition of polysaccharides reduces adsorption quantities while creating a more homogenous and rigid interface. Techniques using CNC coated substrates show promise for studying formulations' interactions with cellulose surfaces.
Article
Chemistry, Physical
Jugal Suthar, Alberto Alvarez-Fernandez, Esther Osarfo-Mensah, Stefano Angioletti-Uberti, Gareth R. Williams, Stefan Guldin
Summary: Extracellular vesicles (EVs) are released from various cell types and contain biomolecular content for health and disease identification. However, the heterogeneity of EVs and complexity of biofluids pose challenges to current analytical approaches. This study explores nanostructuring the sensor electrode surface to enhance detection capabilities and achieves significant improvement.
NANOSCALE HORIZONS
(2023)
Article
Chemistry, Physical
Laure Bar, Martin Eduardo Villanueva, Claudio Martin, Andrea Valencia Ramirez, Jonathan Goole, Frank Uwe Renner, Patricia Losada-Perez
Summary: Hybrid lipid bilayers composed of different types of molecules are suitable for studying biomembrane-related phenomena and can be produced in a controlled array fashion. The formation and stability of hybrid supported membranes are influenced by lipid phase, substrate surface energy, and topography. The lateral organization of hybrid membranes is weaker compared to standard supported lipid bilayers due to suboptimal organization of the underlying self-assembled monolayer. The self-assembled monolayer can undergo non-trivial reorganization in an aqueous environment.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2023)
Article
Cell Biology
Eleni Priglinger, Juergen Strasser, Boris Buchroithner, Florian Weber, Susanne Wolbank, Daniela Auer, Eva Grasmann, Claudia Arzt, Dmitry Sivun, Johannes Grillari, Jaroslaw Jacak, Johannes Preiner, Mario Gimona
Summary: Interest in using mesenchymal stem cell derived extracellular vesicles (MSC-EVs) as therapeutic agents has grown in the past decade. This study established and verified quartz crystal microbalance (QCM) as a highly sensitive label-free immunosensing technique for characterizing clinically approved umbilical cord MSC-EVs. By using QCM along with common characterization methods, specific detection of EVs via EV and MSC markers was achieved, allowing for quantitative determination of the ratio of marker-specific EVs versus non-vesicular particles.
JOURNAL OF EXTRACELLULAR VESICLES
(2021)
Article
Biotechnology & Applied Microbiology
Yuki Terauchi, Megumi Nagayama, Takumi Tanaka, Hiroki Tanabe, Akira Yoshimi, Kei Nanatani, Hiroshi Yabu, Toshihiko Arita, Takeshi Higuchi, Tomoshi Kameda, Keietsu Abe
Summary: The adsorption kinetics of hydrophobin RolA onto solid surfaces and the self-assembled structures formed by RolA molecules were studied. The results showed that the amount of adsorbed RolA, the chemical properties of the surfaces, and the zeta potential of RolA all affected the self-assembled structures formed on the surfaces.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Noel F. Bonet, Daniel G. Cava, Marisela Velez
Summary: Quartz Crystal Microbalance (QCM) with dissipation and Atomic Force Microscopy (AFM) are two characterization techniques that provide kinetic, thermodynamic, and structural information at the nanometer scale of events taking place at solid-liquid interfaces. They are used to describe biological events at supported lipid bilayers (SLBs).
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Food Science & Technology
Fuat Aydin, Tuba Kayman, Secil Abay, Harun Hizlisoy, Izzet Burcin Saticioglu, Emre Karakaya, Orhan Sahin
Summary: This study investigated the genetic diversity, prevalence, and mechanisms of resistance to quinolone antibiotics in 178 Campylobacter jejuni isolates from humans, cattle, dogs, and chickens in Turkey. The results showed that there were 21 clonal complexes (CCs) and 78 different sequence types (STs) among the isolates, with CC21, CC353, CC206, and CC257 being the predominant clones. Quinolone resistance was detected in 41% of the isolates, and all resistant isolates had a specific mutation in the gyrA gene. The study provided important insights into the transmission pathways of C. jejuni in Turkey, suggesting that broiler meat and dogs may be the most important sources of human campylobacteriosis in the country.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2023)
Article
Chemistry, Physical
Ryota Saino, Masaaki Akamatsu, Kenichi Sakai, Hideki Sakai
Summary: Time-dependent morphological changes were observed in cationic monomeric and gemini surfactant mixtures adsorbed on silica using HS-AFM. The wormlike surface aggregates in HTAB solution transformed into a bilayer in an HTAB + 122-12 mixed solution, and this change was reversible when the original HTAB solution was reinstated. These findings were supported by QCM-D and ellipsometry measurements showing reversible energy dissipation in response to solution exchanges.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2021)
Article
Chemistry, Multidisciplinary
David H. Cho, Tian Xie, Patrick J. Mulcahey, Noah P. Kelleher, Jong-in Hahm
Summary: In this study, researchers conducted a detailed investigation on the adsorption and assembly of proteins on polymer surfaces with greater chemical complexity and nanoscopic features. They identified intriguing features and correlated them with adsorption kinetic plots. They provided detailed evidence for protein adsorption pathways on nanoscale polymer surfaces and highlighted the importance of this knowledge for designing biomaterials and biomedical devices.
Article
Biophysics
Anna Sobiepanek, Patrycja D. Kowalska, Magdalena Szota, Tomasz M. Grzywa, Jakub Nowak, Pawel K. Wlodarski, Ryszard Galus, Barbara Jachimska, Tomasz Kobiela
Summary: Melanoma is a life-threatening disease with frequent resistance to treatment. Currently, no single biomarker can accurately predict the aggressiveness of melanoma. Therefore, it is highly important to search for a quantifying method for simultaneous diagnosis and prognosis of melanoma patients.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Electrochemistry
Wolfgang Harreither, Raphael Trouillon, Philippe Poulin, Wilfrid Neri, Andrew G. Ewing, Gulnara Safina
ELECTROCHIMICA ACTA
(2016)
Article
Chemistry, Multidisciplinary
Jani Tuoriniemi, Beatriz Moreira, Gulnara Safina
Article
Chemistry, Analytical
Maria E. Yakovleva, Gulnara R. Safina, Bengt Danielsson
ANALYTICA CHIMICA ACTA
(2010)
Review
Chemistry, Analytical
Gulnara Safina
ANALYTICA CHIMICA ACTA
(2012)
Article
Biochemical Research Methods
Muhammad Nadeem Zafar, Gulnara Safina, Roland Ludwig, Lo Gorton
ANALYTICAL BIOCHEMISTRY
(2012)
Review
Chemistry, Analytical
Yuqing Lin, Raphael Trouillon, Gulnara Safina, Andrew G. Ewing
ANALYTICAL CHEMISTRY
(2011)
Article
Chemistry, Analytical
Wolfgang Harreither, Raphael Trouillon, Philippe Poulin, Wilfrid Neri, Andrew G. Ewing, Gulnara Safina
ANALYTICAL CHEMISTRY
(2013)
Article
Electrochemistry
Gulnara Safina, Roland Ludwig, Lo Gorton
ELECTROCHIMICA ACTA
(2010)
Article
Chemistry, Analytical
Salah Salman, Srimathi Soundararajan, Gulnara Safina, Ikuo Satoh, Bengt Danielsson
Article
Chemistry, Analytical
Gulnara Safina, Margret van Lier, Bengt Danielsson
Article
Chemistry, Analytical
Gulnara Safina, Iu Benet Duran, Mohammed Alasel, Bengt Danielsson
Article
Multidisciplinary Sciences
Rustem Zairov, Asiya Mustafina, Nataliya Shamsutdinova, Irek Nizameev, Beatriz Moreira, Svetlana Sudakova, Sergey Podyachev, Alfia Fattakhova, Gulnara Safina, Ingemar Lundstrom, Aidar Gubaidullin, Alberto Vomiero
SCIENTIFIC REPORTS
(2017)
Article
Chemistry, Analytical
Jani Tuoriniemi, Lo Gorton, Roland Ludwig, Gulnara Safina
ANALYTICAL CHEMISTRY
(2020)
Article
Chemistry, Analytical
Beatriz Moreira, Jani Tuoriniemi, Naghmeh Kouchak Pour, Lydia Mihalcikova, Gulnara Safina
ANALYTICAL CHEMISTRY
(2017)
Article
Chemistry, Analytical
Yujia Ying, Huilin Li
Summary: Enzymes play a crucial role as biological catalysts in accelerating biochemical reactions in living organisms. This study proposes a convenient method for monitoring enzymatic catalytic processes using native top-down mass spectrometry. By exploring the heterogeneity of the chymotrypsin sample and using tandem mass spectrometry, the researchers were able to monitor covalent and noncovalent enzymatic complexes, substrates, and products during catalysis. The results demonstrate that this method has the potential to be a promising tool for characterizing biocatalysts.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Kang Yang, Shuaibo Shi, Jinyu Wu, Shaolong Han, Shengdi Tai, Shishen Zhang, Kun Zhang
Summary: H2O and D2O are important analogues closely related to various industries and monitoring fields. This study successfully distinguishes and detects H2O and D2O by designing a novel eu(III)-macrocycle with dual emitters, Eu-2a, and conducting fluorescence titration experiments.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Julia Kuligowski, Alvaro Perez-Rubio, Marta Moreno-Torres, Polina Soluyanova, Judith Perez-Rojas, Ivan Rienda, David Perez-Guaita, Eugenia Pareja, Ramon Trullenque-Juan, Jose Castell, Marcha Verheijen, Florian Caiment, Ramiro Jover, Guillermo Quintas
Summary: This study introduces a novel variable selection approach called cluster PLS (c-PLS) to assess the joint impact of variable groups selected based on biological characteristics on the predictive performance of a multivariate model. The usefulness of c-PLS is shown using miRNomic and metabolomic datasets obtained from the analysis of liver tissue biopsies.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Meixue Lai, Lijie Zhong, Siyi Liu, Yitian Tang, Tingting Han, Huali Deng, Yu Bao, Yingming Ma, Wei Wang, Li Niu, Shiyu Gan
Summary: This study presents a method for constructing wearable sweat electrolyte sensors using carbon fiber-based solid-contact ion-selective electrodes (SC-ISEs). By using carbon fibers extracted from commercial cloth as electrode material, the cost and reproducibility issues of flexible SC-ISEs were addressed. The results showed that the carbon fiber-based SC-ISEs exhibited reversible voltammetric and stable impedance performances, and had high reproducibility of standard potentials between normal and bending states.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Yang, Ying Liu, Min Deng, Peipei Wang, Dan Cheng, Songjiao Li, Longwei He
Summary: A near-infrared fluorescent probe has been developed to accurately measure the levels of peroxynitrite (ONOO-) in the endoplasmic reticulum (ER) in acute lung injury (ALI). The probe demonstrated rapid response, high selectivity, good sensitivity, and enhanced fluorescence intensity in response to ONOO-. It was successfully used to detect changes in ONOO- levels and showed significant increases in an ALI cell model and an ALI mouse model.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yanping Wang, Yuemei Chen, Kejun Li, Jinrong Zhou, Xin Yuan, Mei Zhang, Ke Huang
Summary: In this work, a portable analytical system based on point discharge chemical vapor generation atomic emission spectrometry (PD-CVG-AES) coupling with gold filament enrichment was designed. The highly sensitive analysis of Hg2+ indirectly realized the detection of ascorbic acid (AA). The measurement is based on the fact that Ag+ can decrease the concentration of Hg2+ by forming Ag-Hg amalgam in the presence of the reductant SnCl2, while AA can pre-reduce Ag+ to Ag0, leading to the generation of silver nanoparticles (Ag NPs). The developed novel analytical strategy broadens the application of microplasma-based AES and offers a higher level of sensitivity compared to current AA detection techniques.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yundi Huang, Bo Song, Kaiwen Chen, Deshu Kong, Jingli Yuan
Summary: This study developed two lysosome-targetable background-free TGL probes for efficient and accurate detection of 1O2, which can be used for monitoring endogenous 1O2 concentrations in lysosomes and discriminating variability induced by different photosensitizers. Furthermore, a smart luminescent sensor film was successfully prepared for on-site 1O2 production detection during PDT processes, offering a promising clinical monitoring tool for skin diseases.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Wei Lang, Jia-Mei Qin, Qian-Yong Cao
Summary: A novel polymer-based probe P1 was successfully synthesized for fluorescently ratiometric sensing of H2S with high selectivity and sensitivity. A smartphone sensing platform was constructed to conduct visual quantitative detection of H2S. P1 can be employed in evaluating the level fluctuations of H2S in living cells, testing water samples/wine samples, and monitoring food freshness.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yuanyuan Qin, Shuda Liu, Shuyun Meng, Dong Liu, Tianyan You
Summary: The study developed a split aptamer-based sandwich-type ratiometric biosensor for the detection of 17 beta-estradiol (E2) using photoelectrochemical and electrochemical assays. The biosensor utilized split aptamer fragments to recognize E2 and trigger a hybridization chain reaction, resulting in the production of double-stranded DNA labeled with CdTe quantum dots. This DNA complex was able to sensitize CdTe quantum dots and generate response signals for E2 detection. The developed biosensor demonstrated high sensitivity and accuracy with two linear ranges and low detection limits.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Siriwan Teepoo, Jongjit Jantra, Khaunnapa Panapong, David Taiwo Ajayi
Summary: A novel immunochromatographic assay based on hyperbranched Au plasmonic blackbodies with a smartphone readout was developed for rapid and sensitive detection of leucomalachite green residues in fish and shrimp products.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Andrea L. Larraga-Urdaz, Borja Moreira-Alvarez, Jorge Ruiz Encinar, Jose M. Costa-Fernandez, Maria Luisa Fernandez-Sanchez
Summary: A major challenge in the 21st century is the development of point-of-care diagnostic tools. In this study, a highly sensitive and simple bioassay using AuNPs and MNAzymes was developed for rapid detection and quantification of miRNA-4739. The proposed strategy shows potential for breast cancer diagnosis.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Xiaohan Zhao, Anyu Wang, Lingzi Zhai, Jiuhe Gao, Sizhe Lyu, Yingshan Jiang, Tian Zhong, Ying Xiao, Xi Yu
Summary: A novel method using polystyrene-coated magnetic nanoparticles for extracting monohydroxy polycyclic aromatic hydrocarbons from urine samples was investigated. The proposed method is simple, sensitive, and efficient, with desirable sensitivity for analyzing low-abundance metabolites in large volumes of complex urine samples.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Quan, Yuqing Zeng, Wenke Zhang, Fengfeng Li, Mengjiao Li, Zhihe Qing, Linlin Wu
Summary: In this study, a visual and reusable biosensor was developed for detecting substrates that are closely associated with human physiological health. The immobilized oxidase showed higher stability and sensitivity under harsh conditions, enabling reliable detection of substrates in complex fluids.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Dongjuan Wang, Xiuqian Ding, Jinling Xie, Juan Wang, Guanhao Li, Xin Zhou
Summary: In this study, a three-in-one sensor was developed for real-time detection of biogenic amines (BAs) with high sensitivity and selectivity. The sensor showed multimodal responses and could be used to fabricate portable devices for on-site non-destructive assessment of food spoilage indicators.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Jinting Meng, Zihao Xu, Shasha Zheng, Hongqun Yang, Tianfu Wang, Hong Wang, Yingwei Zhang
Summary: This study demonstrates the development of a cascade signal amplification system using a multi-pedal DNA walker and strand displacement reactions for the electrochemical detection of miRNA-155. The biosensor exhibited high sensitivity, selectivity, and the potential for clinical applications.
ANALYTICA CHIMICA ACTA
(2024)