Article
Chemistry, Multidisciplinary
Sylvie Egloff, Nina Melnychuk, Elisabete Cruz Da Silva, Andreas Reisch, Sophie Martin, Andrey S. Klymchenko
Summary: The study introduces a method named AmpliFISH for RNA imaging, utilizing small and ultrabright dye-loaded polymeric nanoparticles (NPs) functionalized with DNA. The optimized NPs enabled sequence-specific imaging of different mRNA targets and multiplexed detection of three mRNA targets simultaneously. AmpliFISH provided a stronger signal and shorter processing time compared to commercial locked nucleic acid (LNA)-based FISH technique.
Article
Chemistry, Multidisciplinary
Meirong Cui, Dan Zhang, Qingfu Wang, Jie Chao
Summary: DNAzymes hold great promise as transducing agents for the analysis of intracellular biomarkers. However, their low delivery efficiency and limited signal amplification capability hinder their application in low-abundance biomarker analysis. In this study, a general strategy to design an intelligent, autocatalytic DNAzyme biocircuit is developed for amplified microRNA imaging in living cells. The DNAzyme biocircuit is constructed based on a nanodevice composed of catalytic hairpin assembly (CHA) and DNAzyme biocatalytic functional units, sustained by Au nanoparticles (AuNPs) and MnO2 nanosheets (CD/AM nanodevices). The intelligent autocatalytic multilayer DNAzyme biocircuit can effectively avoid signal leakage and obtain high amplification gain, expanding the application of programmable complex DNA nanocircuits in biosensing, nanomaterial assembly, and biomedicine.
Article
Chemistry, Multidisciplinary
Pu Zhang, Yu Ouyang, Itamar Willner
Summary: In this study, dye-loaded UiO-66 metal-organic framework nanoparticles modified with catalytic hemin/G-quadruplex DNAzyme labels were utilized for chemiluminescence resonance energy transfer (CRET) analysis of miRNAs and genes. The engineered hairpin probes, caged G-quadruplex domains, and self-assembly of hemin/G-quadruplex DNAzyme labels allowed for sensitive detection of specific miRNAs and genes. The DNA hairpin-functionalized dye-loaded NMOF sensing modules were also applied for amplified miRNA or gene CRET-based sensing platforms, demonstrating multiplexed detection capabilities.
Article
Chemistry, Multidisciplinary
Xiaojin Wang, Mikkel B. B. Liisberg, Georgia L. L. Vonlehmden, Xu Fu, Cecilia Cerretani, Lan Li, Lance A. A. Johnson, Tom Vosch, Christopher I. I. Richards
Summary: Understanding the transport of drugs and nanocarriers in cerebrovascular networks is challenging but important for pharmacokinetic and hemodynamic studies. In this study, DNA-stabilized silver nanoclusters were used for multiphoton in vivo fluorescence correlation spectroscopy in live mice, allowing for high-resolution measurement of cerebral blood flow rates. By loading the nanoclusters into liposomes, the fluorescence emission was both bright and stable, enabling quantification of blood flow velocities within individual vessels of the mouse.
Article
Chemistry, Multidisciplinary
Dandan Lei, Qixiang Zhang, Nishuang Liu, Tuoyi Su, Luoxin Wang, Ziqi Ren, Yihua Gao
Summary: A self-powered all-solid-state pressure sensing system rGM has been developed based on a mechanical potentiometric transduction mechanism, showing excellent performance in converting mechanical stimulation into stable voltage and current. It has a wide detection range, high stability, and potential impact on healthcare.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Article
Chemistry, Analytical
Jie Liu, Dianping Tang
Summary: This study presents a simple and novel electrochemical immunoassay based on MXene (Ti3C2)-Au nanoparticles for sensitive screening of the disease-related biomarker prostate-specific antigen (PSA). The method utilizes dopamine-loaded liposomes for signal amplification. The results demonstrate that the developed immunoassay exhibits a broad linear range and low detection limit, making it a promising detection platform for clinical sample analysis.
Article
Nanoscience & Nanotechnology
Zekun Li, Shuang Liang, Huijuan Sun, Cheng Bao, Yuan Li
Summary: The study demonstrates that liposomes loaded with rutin can improve its solubility and cellular uptake, and the incorporation of these liposomes into a microneedle patch system enhances the local delivery of rutin to adipocytes. The microneedle patch formulation shows the most significant weight reduction in a high-fat diet mouse model by downregulating lipid synthesis-related proteins and promoting beige adipogenesis-related proteins. The study suggests that this liposome-based microneedle patch system is a promising approach to enhance the antiobesity efficacy of rutin.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Analytical
Jiaxin Su, Jinya Du, Rujiao Ge, Chenyang Sun, Yuchun Qiao, Wei Wei, Xuejiao Pang, Yufan Zhang, Huiting Lu, Haifeng Dong
Summary: This study demonstrates a novel DNAzyme-based self-driven intracellular sensor for miRNA detection in living cells. The sensor utilizes a metal-organic framework core to efficiently deliver and protect the DNAzyme, and employs pH-responsive degradation to release the DNAzyme and cofactors for miRNA detection.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Daxiu Li, Yusi Li, Ruo Yuan, Yun Xiang
Summary: The study proposes an amplified FRET sensing strategy for sensitive detection of Myc-Max in human serums based on a DNA translator probe, DNAzyme, and clamped hybridization chain reaction (cHCR)-integrated signal amplification. The developed method can also sensitively discriminate low levels of Myc-Max in diluted human serums, making it a versatile tool for early-stage disease diagnosis.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Multidisciplinary
Wen-Hsin Chang, Yi-Fang Lee, Yen-Wenn Liu, Itamar Willner, Wei-Ching Liao
Summary: A method for synthesizing DNA-based acrylamide hydrogel microcapsules loaded with quantum dots as readout signal is introduced, which can detect cancer-associated microRNAs. The detection sensitivity can be improved by coupling the microcapsules with strand displacement amplification.
Article
Chemistry, Analytical
Wen Yi Lv, Chun Hong Li, Yuan Fang Li, Shu Jun Zhen, Cheng Zhi Huang
Summary: The three-layer hierarchical hybridization chain reaction (3L hHCR) proposed in this work achieves robust signal amplification efficiency and ultra-sensitive detection, with the ability to discriminate different concentrations of target sequences. It can also be operated as a cascade AND logic gate and a molecular keypad lock, showing potential applications in cancer diagnosis and biosensing.
ANALYTICAL CHEMISTRY
(2021)
Article
Nanoscience & Nanotechnology
Motoi Oishi, Shotaro Juji
Summary: Accelerated DNA hybridization chain reactions (HCRs) using DNA origami as a scaffold have attracted attention in dynamic DNA nanotechnology. In this study, semilocalized and localized HCR systems were constructed using magnetic beads (MBs) as a simple scaffold for enzyme-free miR-21 detection. The DNA density on MBs was found to be crucial for HCR kinetics and limit of detection.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Chemistry, Multidisciplinary
Fenfen Zheng, Tiantian Meng, Difei Jiang, Jiamin Sun, Haiyang Yao, Jun-Jie Zhu, Qianhao Min
Summary: The study presents a nanomediator-effector cascade system for in situ amplification of kinase-catalyzed phosphorylation events, enabling kinase activity imaging and responsive drug release. This system offers a kinase-specific sense-and-treat paradigm for cancer theranostics.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Chemistry, Multidisciplinary
Jahwarhar Izuan Abdul Rashid, Nor Azah Yusof, Jaafar Abdullah, Rafidah Hanim Shomiad Shueb
Summary: This study focuses on treating genomic dengue virus gene samples for use in electrochemical DNA biosensors, using methylene blue as a redox indicator. Different MB signals were generated during electrochemical sensing of non-amplified vs. amplified samples. The results demonstrate the successful discrimination of MB current signals in the presence vs. absence of the target genomic dengue virus.
Article
Chemistry, Multidisciplinary
Xi Long, Jiacheng Li, Tong Luo, Hui Liu, Zhiwei Deng, Jiacheng Ding, Zan Gong, Yanjing Yang, Shian Zhong
Summary: In this work, a highly sensitive, specific, and versatile method called CRISPR-CRT was developed to quantify miRNA expression. The method involves the conversion and amplification of miRNA targets into hairpin RT products, which then activate the collateral cleavage activity of CRISPR-Cas12a. The two-stage amplification enables the detection of miRNA at a sub-femtomolar level and also provides excellent specificity in detecting miR-21. The method has been successfully used to assess miR-21 levels in human serum samples and detect disease-specific serum.
CHEMICAL COMMUNICATIONS
(2023)
Article
Chemistry, Multidisciplinary
Wenyue Gao, Elena Zdrachek, Xiaojiang Xie, Eric Bakker
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2020)
Article
Chemistry, Analytical
Tara Forrest, Elena Zdrachek, Eric Bakker
Biographical-Item
Electrochemistry
Gerd-Uwe Flechsig
JOURNAL OF SOLID STATE ELECTROCHEMISTRY
(2020)
Editorial Material
Chemistry, Multidisciplinary
J. Justin Gooding, Antonella Mazur, Eric Bakker, Shana Kelley, Nongjian Tao, Michael Sailor, Maarten Merkx, Lanqun Mao, Heather Clark
Article
Chemistry, Multidisciplinary
Wenyue Gao, Xiaojiang Xie, Eric Bakker
Article
Chemistry, Multidisciplinary
Pitchnaree Kraikaew, Stephane Jeanneret, Yoshiki Soda, Thomas Cherubini, Eric Bakker
Article
Chemistry, Analytical
Sarasi K. K. Galagedera, Gerd-Uwe Flechsig
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2020)
Editorial Material
Chemistry, Multidisciplinary
J. Justin Gooding, Antonella Mazur, Eric Bakker, Shana Kelley, Michael Sailor, Maarten Merkx, Lanqun Mao, Heather Clark, Roya Maboudian, Yitao Long
Editorial Material
Chemistry, Multidisciplinary
Eric Bakker, Ciara K. O'Sullivan, Paul S. Cremer
Article
Chemistry, Analytical
Sutida Jansod, Thomas Cherubini, Yoshiki Soda, Eric Bakker
ANALYTICAL CHEMISTRY
(2020)
Article
Chemistry, Multidisciplinary
Eric Bakker
Article
Chemistry, Analytical
Mary-Lou Tercier-Waeber, Marianna Fighera, Melina Abdou, Eric Bakker, Peter van der Wal
Summary: This study developed a new gel-integrated nanostructured gold-based microelectrode array for direct detection of inorganic arsenite in fresh and marine aquatic systems. The sensors demonstrated sub-nanomolar detection and quantification limits, making them suitable for remote high-resolution monitoring underwater.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Elena Zdrachek, Eric Bakker
Summary: Solid-contact ion-selective electrodes with thin membranes can achieve complete ion exchange and unbiased selectivity coefficients. The reconditioning rate corresponds well to experimental data. Alternatively, the redox state of the conducting polymer can be electrochemically switched to expel primary ions and uptake interfering ions.
Editorial Material
Chemistry, Multidisciplinary
J. Justin Gooding, Nicole Camasso, Eric Bakker, Shana Kelley, Michael Sailor, Maarten Merkx, Lanqun Mao, Heather Clark, Roya Maboudian, Jean-Francois Masson, Andrew deMello, Meng Li, Sue Min Liu
Review
Chemistry, Analytical
Elena Zdrachek, Eric Bakker
ANALYTICAL CHEMISTRY
(2021)