Article
Microbiology
Hamid Staji, Mohammadhasan Yousefi, Mahmoud Ahmadi Hamedani, Iradj Ashrafi Tamai, Sahar Ghaffari Khaligh
Summary: This study reported the first occurrence of A. capra in wild onagers and suggested that equines could be infected with and act as reservoirs for A. capra.
VETERINARY MICROBIOLOGY
(2021)
Article
Agronomy
Weier Cui, Alan Zamorano, Nicolas Quiroga, Assunta Bertaccini, Nicola Fiore
Summary: Research has shown that the SSU12p and LSU36p genes are reliable additional markers for detection and differentiation of phytoplasmas.
PHYTOPATHOLOGIA MEDITERRANEA
(2021)
Article
Biotechnology & Applied Microbiology
Samart Dorn-In, Sirkka Mang, Karin Schwaiger
Summary: This study investigated cold-tolerant Clostridium spp. strains isolated from various samples, identifying them into different groups based on genetic and biochemical characteristics. Spiked beef samples showed changes in odor after storage, while maintaining similar color and meat quality compared to the control group.
Article
Biology
Hamieh Goshtasbi, Ehsan Atazadeh, Ali Movafeghi
Summary: Instability of morphological and physiological characteristics under different environmental conditions can lead to misidentification of cyanobacteria. This study successfully identified three cyanobacteria species from an Iranian wetland using morphological, molecular, and biochemical data. The results confirmed the effectiveness of molecular markers and biochemical parameters in reliable identification of cyanobacteria.
Article
Medicine, General & Internal
Martina C. Agbo, Kenneth Ugwu, Boniface N. Ukwah, Ifeoma M. Ezeonu
Summary: This study evaluated the genetic relatedness of MDR clinical isolates of P. aeruginosa. The results showed a high prevalence of MDR P. aeruginosa in the Nskuka area and a high degree of genetic relatedness among the isolates.
AFRICAN HEALTH SCIENCES
(2023)
Article
Agriculture, Dairy & Animal Science
Hongfa Zhang, Chunping You
Summary: This study developed a new PCR method using the CRISPR-Cas9 system to remove fermentative bacteria in yogurt and enrich unintended bacterial contaminants. The method improved detection sensitivity, simplified operation steps, and reduced detection costs. By analyzing unintended bacterial contaminants in yogurt with shrunken packaging, the underlying reasons for product quality issues were identified.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Agriculture, Dairy & Animal Science
Livio Galosi, Nicola Ridolfi, Cristina Fellini, Igor Pelizzone, Stefano Cusaro, Gianluca Marchetti, Matteo Canonico, Elena Ghelfi, Nicola Di Girolamo, Silvia Preziuso
Summary: Some species of turtles and tortoises are threatened by pathogens such as Mycoplasmopsis agassizii, which can lead to population declines. Proper diagnosis and control of transmissible diseases are crucial for limiting the spread of pathogens in animal collections. This study highlights the importance of practitioners in collecting and sharing data for analysis and emphasizes the need to collect appropriate samples to avoid false negative results. It also suggests that other pathogens should be considered in coinfection or infection cases.
Article
Plant Sciences
Agron Bunjaku, Zeinab Alsaheli, Natasa Mehle, Bekri Xhemali, Gazmend Gjinovci, Arben Mehmeti, Toufic Elbeaino
Summary: This study is the first report on the presence and molecular characterization of phytoplasmas in Kosovar vineyards, with the phytoplasmas belonging to the 16SrXII group and showing similarity to 'Candidatus Phytoplasma solani'. It is recommended that testing for phytoplasma be included in the certification program for grapevine in Kosovo.
JOURNAL OF PHYTOPATHOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Julia B. Gontijo, Fabiana S. Paula, Andressa M. Venturini, Caio A. Yoshiura, Clovis D. Borges, Jose Mauro S. Moura, Brendan J. M. Bohannan, Klaus Nusslein, Jorge L. Mazza Rodrigues, Siu M. Tsai
Summary: The Amazonian floodplain forests play a crucial role in regional hydrological and biogeochemical cycles, contributing significantly to the global carbon balance through CH4 emissions. Unique geochemical factors may drive the composition of microbial communities, affecting CH4 emissions. Additionally, environmental changes in floodplain areas can impact the diversity of methanotrophic communities.
Article
Biochemistry & Molecular Biology
Peter Braun, Martin Duy-Thanh Nguyen, Mathias C. Walter, Gregor Grass
Summary: A real-time PCR assay targeting a specific single nucleotide polymorphism in Bacillus anthracis genomes was validated, showing high specificity, linearity, and sensitivity. Adapting the assay for reverse transcription PCR on 16S rRNA transcripts significantly increased sensitivity compared to DNA-targeting assays, offering great potential for molecular diagnostics.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Infectious Diseases
Mohamed El-Badawy, Fatma Abou-Elazm, Mohamed S. Omar, Mostafa El-Naggar, Ibrahim A. Maghrabi
Summary: This study is the first to investigate plasmid-borne aminoglycoside and sulfonamide resistance genes among clinical isolates of A. baumannii in Saudi Arabia. A novel allelic variant for aac(6’)-Ib was detected, along with novel mutations in the sul1 gene.
INFECTION AND DRUG RESISTANCE
(2021)
Article
Entomology
Sujie Du, Fuyu Ye, Qijing Wang, Yongxuan Liang, Weijie Wan, Jianyang Guo, Wanxue Liu
Summary: This study finds that the thelytoky of Diglyphus wani is not linked with the presence of endosymbionts.
Article
Infectious Diseases
Yaqun Yan, Chenyang Lu, Pihong Gong, Zhiyang Pei, Yongshuai Peng, Fuchun Jian, Rongjun Wang, Longxian Zhang, Meng Qi, Changshen Ning
Summary: This study aimed to investigate the prevalence and phylogeny of A. phagocytophilum and related strains in small ruminants and ticks in China. The results showed that A. phagocytophilum-like 1 and 2 variants were widespread in sampled animals, with prevalence rates of 22.6% and 0.7% respectively. Phylogenetic analysis based on the groEL gene revealed distinct clades for A. phagocytophilum-like 1 and 2 strains, related to Japanese and Chinese strains of A. phagocytophilum.
TICKS AND TICK-BORNE DISEASES
(2022)
Article
Microbiology
Behcet Kemal Caglar, Serkan Pehlivan, Ekrem Atakan, Toufic Elbeaino
Summary: In two corn fields in Imamoglu town, Turkey, maize plants showed phytoplasma-like symptoms. Through PCR and sequence analysis, it was identified as Ligustrum witches' broom phytoplasma (LiWBP) with a distant relationship to 16SrII and 16SrXXV groups. This is the first report of LiWBP infecting maize and causing phyllody disease in Turkey, providing new molecular insights into its phylogenetic position.
Article
Immunology
Hyo Lim Hong, Laure Flurin, Matthew J. Thoendel, Matthew J. Wolf, Matthew P. Abdel, Kerryl E. Greenwood-Quaintance, Robin Patel
Summary: This study compared the performance of 16S rRNA gene-based targeted metagenomic sequencing (tNGS) and shotgun metagenomic sequencing (sNGS) in detecting and identifying pathogens in sonicate fluid from failed total hip arthroplasty (THA) and total knee arthroplasty (TKA). The study found that tNGS had a higher positive percent agreement with sonicate fluid culture compared to sNGS, and detected potential pathogens in culture-negative PJIs. The study concludes that tNGS is a potential diagnostic tool for identifying PJI pathogens in sonicate fluid, with similar performance characteristics to sNGS.
CLINICAL INFECTIOUS DISEASES
(2023)
Article
Dentistry, Oral Surgery & Medicine
Magda Feres, Belen Retamal-Valdes, Daiane Fermiano, Marcelo Faveri, Luciene Cristina Figueiredo, Marcia Mayer, Jung-Jin Lee, Kyle Bittinger, Flavia Teles
Summary: This study used next-generation sequencing to evaluate the subgingival microbial composition of young patients with severe periodontitis treated with scaling and root planing and systemic metronidazole and amoxicillin. The results showed that mechanical treatment associated with metronidazole and amoxicillin promoted a beneficial change in the microbiome of young individuals with severe periodontitis.
JOURNAL OF PERIODONTOLOGY
(2021)
Article
Dentistry, Oral Surgery & Medicine
Karin H. Ishikawa, Manuela R. Bueno, Dione Kawamoto, Maria R. L. Simionato, Marcia P. A. Mayer
Summary: The study evaluated the effect of postbiotics released by probiotic lactobacilli on Aggregatibacter actinomycetemcomitans (Aa) biofilms and the expression of virulence-associated genes, showing that certain lactobacilli postbiotics may help reduce colonization levels of Aa and modulate its expression of virulence factors.
MOLECULAR ORAL MICROBIOLOGY
(2021)
Article
Engineering, Biomedical
Ana Carolina Costa da Mota, Marcela Leticia Leal Goncalves, Anna Carolina Ratto Tempestini Horliana, Alessandro Melo Deana, Lisyanne Araujo de Souza Cavalcante, Andrea Oliver Gomes, Marcia Pinto Alves Mayer, Ellen Sayuri Ando Suguimoto, Kristianne Porta Santos Fernandes, Raquel Agnelli Mesquita-Ferrari, Renato Araujo Prates, Lara Jansiski Motta, Sandra Kalil Bussadori
Summary: The study found that aPDT using a red LED and 0.005% methylene blue resulted in an immediate reduction in halitosis, but the effect was not maintained after 7, 14, or 30 days. No reduction occurred in the number of bacteria investigated at the microbiological level.
LASERS IN MEDICAL SCIENCE
(2022)
Article
Biotechnology & Applied Microbiology
Glauber Campos Vale, Brenda Izabela Santana Mota, Ellen Sayuri Ando-Suguimoto, Marcia Pinto Alves Mayer
Summary: This study evaluated the effect of probiotic lactobacilli on the gene transcription of antibacterial response in monocytes. The results showed that Lactobacillus acidophilus LA-5 promoted the production of inflammatory factors and enhanced the antibacterial activity of innate defense in periodontal tissues, but it may also increase inflammatory response.
PROBIOTICS AND ANTIMICROBIAL PROTEINS
(2023)
Article
Microbiology
Emmanuel Albuquerque-Souza, Karin Hitomi Ishikawa, Pamela Penas Amado, Jacques Robert Nicoli, Marinella Holzhausen, Marcia P. A. Mayer
Summary: The study found that potential probiotics L. casei 324 m and B. pseudolongum 119(1A) can enhance re-epithelialization and cell proliferation of gingival epithelial cells while inhibiting the harmful effects of periodontal pathogens, P. gingivalis W83, through increasing the expression and release of CXCL8 and the number of cells positive for CXCR1/CXCR2.
Article
Microbiology
Manuela R. Bueno, Karin H. Ishikawa, Gislane Almeida-Santos, Ellen S. Ando-Suguimoto, Natali Shimabukuro, Dione Kawamoto, Marcia P. A. Mayer
Summary: Probiotics may help achieve a balanced microbiome in periodontitis by interfering with the colonization of Aggregatibacter actinomycetemcomitans in the oral cavity. This in vitro study evaluated the effect of two commercially available lactobacilli strains on gingival epithelial cells infected with A. actinomycetemcomitans. The results suggest that these probiotics may reduce the ability of A. actinomycetemcomitans to interact with gingival epithelial cells and modulate the cells' response.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Chemistry, Medicinal
Bruno Bueno-Silva, Manuela Rocha Bueno, Dione Kawamoto, Renato C. Casarin, Joao Marcos Spessoto Pingueiro, Severino Matias Alencar, Pedro Luiz Rosalen, Marcia Pinto Alves Mayer
Summary: (3S)-vestitol, derived from red propolis, exhibits anti-inflammatory, antimicrobial, and anti-caries activity. This study explores the anti-inflammatory mechanisms of (3S)-vestitol on macrophages and reveals its potential as a candidate for the treatment/prevention of persistent inflammatory diseases such as atherosclerosis and periodontitis, involving the inhibition of cytokines and the NF-kappa B pathway.
Editorial Material
Immunology
Marcela Hernandez, Marcia Pinto Alves Mayer, Julien Santi-Rocca
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Gabriela de Morais Gouvea Lima, Celina Faig Lima Carta, Aline Chiodi Borges, Thalita Mayumi Castaldelli Nishime, Cezar Augusto Villela da Silva, Marcelo Vidigal Caliari, Marcia Pinto Alves Mayer, Konstantin Georgiev Kostov, Cristiane Yumi Koga-Ito
Summary: The study evaluated the effects of helium cold atmospheric pressure plasma jet on oral cells, specifically its inhibitory effect on P. gingivalis biofilm and its genotoxicity on epithelial gingival cells and oral fibroblasts.
APPLIED SCIENCES-BASEL
(2022)
Article
Biology
Ana C. C. Pereira, Alana P. S. Aguiar, Leticia M. P. Araujo, Larissa O. Dantas, Marcia P. A. Mayer, Lamprini Karygianni, Thomas Thurnheer, Ericka T. Pinheiro
Summary: This study found that the combination of antibiofilm agents LL-37 and D-amino acids with antibiotics can have some antimicrobial activity. However, compared to complex biofilms, the combination of antibiotics and D-amino acids did not exhibit significant activity.
Article
Biotechnology & Applied Microbiology
Glauber Campos Vale, Brenda Izabela Santana Mota, Ellen Sayuri Ando-Suguimoto, Marcia Pinto Alves Mayer
Summary: This study aimed to evaluate the antibacterial gene expression of dendritic cells challenged with LPS and probiotics. The results showed that LPS upregulated more genes related to inflammation and immune response compared to the groups where probiotics were added. Although probiotics decreased several genes upregulated by LPS, they still upregulated some genes encoding cytokines. Additionally, one specific probiotic promoted the secretion of these proteins.
PROBIOTICS AND ANTIMICROBIAL PROTEINS
(2023)
Article
Dentistry, Oral Surgery & Medicine
Andre L. G. Moreira, Pedro H. F. Silva, Sergio L. Salvador, Karin H. Ishikawa, Graziele C. Ferreira, Jose E. Tanus-Santos, Marcia P. A. Mayer, Sergio L. S. de Souza, Flavia A. C. Furlaneto, Michel R. Messora
Summary: This study evaluated the systemic and local impact of probiotic therapy on rats with metabolic syndrome and periodontitis. The results showed that probiotics can reduce the severity of periodontitis, and modulate lipogenic genes and intestinal morphological and microbiological parameters in rats with metabolic syndrome.
JOURNAL OF PERIODONTOLOGY
(2023)
Article
Dentistry, Oral Surgery & Medicine
Andre L. G. Moreira, Giselle A. Silva, Pedro H. F. Silva, Sergio L. Salvador, Raphael M. Vicente, Graziele C. Ferreira, Jose E. Tanus-Santos, Marcia P. A. Mayer, Karin H. Ishikawa, Sergio Luis Scombatti de Souza, Flavia A. C. Furlaneto, Michel R. Messora
Summary: This study found that Bifidobacterium animalis subspecies lactis HN019 can reduce the sequelae of experimental periodontitis in rats by modulating systemic parameters. Bifidobacterium lactis HN019 can decrease dyslipidemia, insulin resistance, proteinuria levels, systolic blood pressure, hepatic steatosis, and expression of adipokines, as well as improve intestinal microbiota imbalance and prevent alveolar bone loss. These findings highlight the importance of Bifidobacterium lactis HN019 in preventing the damage caused by periodontitis.
JOURNAL OF PERIODONTAL RESEARCH
(2023)
Article
Biotechnology & Applied Microbiology
Mateus Kawata Salgaco, Fellipe Lopes de Oliveira, Adilson Sartoratto, Victoria Mesa, Marcia Pinto Alves Mayer, Katia Sivieri
Summary: Type 2 diabetes is associated with dysbiosis in the gut, which can cause inflammation throughout the body. Probiotic use, specifically Lactobacillus acidophilus-La5, was shown to positively impact the gut microbiome of individuals with type 2 diabetes, leading to potential improvements in glycemic control.
FERMENTATION-BASEL
(2023)
Article
Multidisciplinary Sciences
Carolina Martins Nascimento, Mateus Campos Casaro, Evelyn Roxana Perez, Willian Rodrigues Ribeiro, Marcia Pinto Alves Mayer, Karin Hitomi Ishikawa, Adriana Lino-dos-Santos-Franco, Joice Naiara Bertaglia Pereira, Caroline Marcantonio Ferreira
Summary: Our study investigated the influence of OVA-induced allergic airway inflammation on gut homeostasis, and found that challenges in the respiratory system not only resulted in allergic airway inflammation but also in the loss of gut homeostasis.
