Article
Biochemistry & Molecular Biology
Kartheyaene Jayaprakash Demirel, Rongrong Wu, Alessandra Neves Guimaraes, Isak Demirel
Summary: This study investigated the role of leukotoxin, caspase-1, caspase-4, and NLRP3 in the immune response of gingival epithelial cells during Aggregatibacter actinomycetemcomitans infection. The results showed that the highly leukotoxic strain induced higher release of inflammatory mediators and cell death, which were dependent on caspase-1, caspase-4, and NLRP3. Furthermore, the absence of NLRP3 resulted in significantly different release of inflammatory proteins in infected cells compared to normal cells.
Article
Immunology
Zihan Li, Qiuxia Huang, Zhuoran Wang, Lijia Huang, Lisha Gu
Summary: Periodontitis is a dysbiosis condition caused by the coordinated action of multiple members within the microbial consortium. This study demonstrates that Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans can alter the structure and composition of subgingival microbiome, leading to dysbiosis and impaired epithelium function. P. gingivalis drives the commensal microbiome towards dysbiosis, while A. actinomycetemcomitans enhances the symbiotic state.
JOURNAL OF INFECTIOUS DISEASES
(2023)
Article
Cell Biology
Mariana Rates Gonzaga Santos, Ian de Meira Chaves, Celso Martins Queiroz-Junior, Allysson Thiago Cramer, Thales Augusto Anestino, Anna Clara Paiva Menezes dos Santos, Paulo Gaio Leite, Soraia Macari, Breno Rocha Barrioni, Marivalda de Magalhaes Pereira, Mauro Martins Teixeira, Danielle da Gloria de Souza, Mila Fernandes Moreira Madeira, Fabiana Simao Machado
Summary: This study investigated the role of SOCS2 in Aa-induced alveolar bone loss. Alveolar bone loss was induced by infecting mice with Aa and bone parameters, cell counts, and cytokine profile were evaluated. Results showed that SOCS2 deficiency increased alveolar bone loss despite decreased proinflammatory cytokine production. Therefore, SOCS2 is a regulator of Aa-induced bone loss and an important target for preventing alveolar bone loss in periodontal inflammatory conditions.
INFLAMMATION RESEARCH
(2023)
Article
Microbiology
Daniel Betancur, Camila Munoz Grez, Angel Onate
Summary: This study evaluated the response of oral keratinocytes and macrophages to Aggregatibacter actinomycetemcomitans, finding that different serotypes of the bacteria induced increased expression of cytokines and related molecules in the cells at various time points after infection. Keratinocytes and macrophages contribute to early inflammation in periodontitis, acting as the first signal for immune cell recruitment and modulation of immune responses.
Article
Microbiology
Yohei Nakamura, Koji Watanabe, Yoshie Yoshioka, Wataru Ariyoshi, Ryota Yamasaki
Summary: The effect of hydrogen peroxide on Aggregatibacter actinomycetemcomitans was investigated. The treatment resulted in the survival of approximately 0.5% of the bacterial population. Surviving bacteria did not acquire resistance to hydrogen peroxide but exhibited persister behavior. Sterilization with mitomycin C reduced the number of persister survivors.
Review
Dentistry, Oral Surgery & Medicine
Nabil Khzam, Leticia Algarves Miranda, Omar Kujan, Kate Shearston, Dorte Haubek
Summary: This study investigated the global prevalence of the JP2 genotype of Aggregatibacter actinomycetemcomitans (Aa). The results showed a relatively high presence of the JP2 genotype in subjects from South America, North America, and Africa. However, there were no studies estimating the presence of the JP2 genotype in the Oceania region. Due to the heterogeneity and quality of the included publications, caution should be exercised when interpreting the data, and there is a need for additional evidence.
CLINICAL ORAL INVESTIGATIONS
(2022)
Article
Dentistry, Oral Surgery & Medicine
Christian Damgaard, Anne Katrine Danielsen, Christian Enevold, Jesper Reinholdt, Palle Holmstrup, Claus H. Nielsen, Laura Massarenti
Summary: This study revealed a high prevalence of Aa in the saliva of patients with periodontitis-B or periodontitis-C. Serum antibodies against LtxA can distinguish patients with periodontitis from healthy controls.
JOURNAL OF PERIODONTOLOGY
(2021)
Article
Medicine, General & Internal
Klara Martinsson, Andrea Di Matteo, Carina Ohman, Anders Johansson, Anna Svard, Kulveer Mankia, Paul Emery, Alf Kastbom
Summary: This study aimed to determine the presence of antibodies towards LtxA in patients at risk of developing rheumatoid arthritis (RA). The results showed that serum anti-LtxA levels were not elevated before RA diagnosis, and associations with disease progression and ACPA levels differed between populations.
FRONTIERS IN MEDICINE
(2023)
Article
Immunology
Hazel Ozuna, Silvia M. Uriarte, Donald R. Demuth
Summary: This study revealed that human neutrophils can synthesize, store, and release epinephrine, which A. actinomycetemcomitans relies on as a growth signal. The bacteria promotes azurophilic granule exocytosis by neutrophils to acquire epinephrine for survival, providing new insights into the mechanism of bacterial infection in periodontitis.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Microbiology
Zeinab Razooqi, Carola Hoglund Aberg, Francis Kwamin, Rolf Claesson, Dorte Haubek, Jan Oscarsson, Anders Johansson
Summary: The study aimed to investigate the presence of two oral pathogens and their genotypes in relation to the development of periodontal disease. The results show that the presence of Aggregatibacter actinomyctemcomitans and Filifactor alocis is associated with increased periodontal pocket depth and progression of the disease.
Article
Biochemistry & Molecular Biology
Yuri Song, Jin Chung
Summary: This study aimed to investigate the molecular mechanism and anti-inflammatory function of zingerone on periodontal inflammation induced by A. actinomycetemcomitans. Zingerone inhibited nitric oxide (NO) production and the expression of pro-inflammatory cytokines in THP-1 macrophages. It also enhanced autophagosome formation and reduced the intracellular survival of A. actinomycetemcomitans. These findings suggest that zingerone has potential as a treatment for periodontal inflammation.
Article
Microbiology
Rolf Claesson, Jan Oscarsson, Anders Johansson
Summary: The JP2 genotype of A. actinomycetemcomitans is associated with aggressive forms of periodontitis and can be detected in subgingival plaque samples from adolescents worldwide. The dissemination of this genotype follows migration routes and can now be found in periodontitis patients in many countries.
Article
Microbiology
Rolf Claesson, Anders Johansson, Georgios N. Belibasakis
Summary: The aim of this study was to compare the prevalence and proportions of three bacterial species in periodontitis pocket samples from young and old patients. The results showed that the prevalence of Aggregatibacter actinomycetemcomitans was higher in young patients, while Porphyromonas gingivalis was similarly distributed in both age groups. Parvimonas micra was more prevalent in old patients. Furthermore, the proportion of samples containing A. actinomycetemcomitans and P. micra together was higher than when P. gingivalis was present.
Article
Dentistry, Oral Surgery & Medicine
Karin H. Ishikawa, Manuela R. Bueno, Dione Kawamoto, Maria R. L. Simionato, Marcia P. A. Mayer
Summary: The study evaluated the effect of postbiotics released by probiotic lactobacilli on Aggregatibacter actinomycetemcomitans (Aa) biofilms and the expression of virulence-associated genes, showing that certain lactobacilli postbiotics may help reduce colonization levels of Aa and modulate its expression of virulence factors.
MOLECULAR ORAL MICROBIOLOGY
(2021)
Article
Dentistry, Oral Surgery & Medicine
Camila S. Stolf, Catharina M. Sacramento, Carolina A. P. G. Alvarenga, Jonathan R. Vieira, Cassia F. Araujo, Mabelle F. Monteiro, Helvis E. S. Paz, Mauro P. Santamaria, Karina G. S. Ruiz, Renato C. Casarin
Summary: This study aims to assess the local immune response of Perio4C patients by exposing primary gingival fibroblast cells (GFs) to Aggregatibacter actinomycetemcomitans protein extract (AaPE) and quantifying the secretion of inflammatory cytokines IL-4, IL-17, TNF-alpha, IL-1 beta, IFN-gamma, and IL-10 super-family members (IL-10, IL-19, and IL-24) by them. The results showed that Perio4C GFs had lower production of certain pro- and anti-inflammatory molecules, leading to a pro-inflammatory extracellular environment.
JOURNAL OF PERIODONTOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Glauber Campos Vale, Brenda Izabela Santana Mota, Ellen Sayuri Ando-Suguimoto, Marcia Pinto Alves Mayer
Summary: This study evaluated the effect of probiotic lactobacilli on the gene transcription of antibacterial response in monocytes. The results showed that Lactobacillus acidophilus LA-5 promoted the production of inflammatory factors and enhanced the antibacterial activity of innate defense in periodontal tissues, but it may also increase inflammatory response.
PROBIOTICS AND ANTIMICROBIAL PROTEINS
(2023)
Article
Chemistry, Medicinal
Bruno Bueno-Silva, Manuela Rocha Bueno, Dione Kawamoto, Renato C. Casarin, Joao Marcos Spessoto Pingueiro, Severino Matias Alencar, Pedro Luiz Rosalen, Marcia Pinto Alves Mayer
Summary: (3S)-vestitol, derived from red propolis, exhibits anti-inflammatory, antimicrobial, and anti-caries activity. This study explores the anti-inflammatory mechanisms of (3S)-vestitol on macrophages and reveals its potential as a candidate for the treatment/prevention of persistent inflammatory diseases such as atherosclerosis and periodontitis, involving the inhibition of cytokines and the NF-kappa B pathway.
Editorial Material
Immunology
Marcela Hernandez, Marcia Pinto Alves Mayer, Julien Santi-Rocca
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Gabriela de Morais Gouvea Lima, Celina Faig Lima Carta, Aline Chiodi Borges, Thalita Mayumi Castaldelli Nishime, Cezar Augusto Villela da Silva, Marcelo Vidigal Caliari, Marcia Pinto Alves Mayer, Konstantin Georgiev Kostov, Cristiane Yumi Koga-Ito
Summary: The study evaluated the effects of helium cold atmospheric pressure plasma jet on oral cells, specifically its inhibitory effect on P. gingivalis biofilm and its genotoxicity on epithelial gingival cells and oral fibroblasts.
APPLIED SCIENCES-BASEL
(2022)
Article
Biology
Ana C. C. Pereira, Alana P. S. Aguiar, Leticia M. P. Araujo, Larissa O. Dantas, Marcia P. A. Mayer, Lamprini Karygianni, Thomas Thurnheer, Ericka T. Pinheiro
Summary: This study found that the combination of antibiofilm agents LL-37 and D-amino acids with antibiotics can have some antimicrobial activity. However, compared to complex biofilms, the combination of antibiotics and D-amino acids did not exhibit significant activity.
Article
Biotechnology & Applied Microbiology
Glauber Campos Vale, Brenda Izabela Santana Mota, Ellen Sayuri Ando-Suguimoto, Marcia Pinto Alves Mayer
Summary: This study aimed to evaluate the antibacterial gene expression of dendritic cells challenged with LPS and probiotics. The results showed that LPS upregulated more genes related to inflammation and immune response compared to the groups where probiotics were added. Although probiotics decreased several genes upregulated by LPS, they still upregulated some genes encoding cytokines. Additionally, one specific probiotic promoted the secretion of these proteins.
PROBIOTICS AND ANTIMICROBIAL PROTEINS
(2023)
Article
Dentistry, Oral Surgery & Medicine
Andre L. G. Moreira, Pedro H. F. Silva, Sergio L. Salvador, Karin H. Ishikawa, Graziele C. Ferreira, Jose E. Tanus-Santos, Marcia P. A. Mayer, Sergio L. S. de Souza, Flavia A. C. Furlaneto, Michel R. Messora
Summary: This study evaluated the systemic and local impact of probiotic therapy on rats with metabolic syndrome and periodontitis. The results showed that probiotics can reduce the severity of periodontitis, and modulate lipogenic genes and intestinal morphological and microbiological parameters in rats with metabolic syndrome.
JOURNAL OF PERIODONTOLOGY
(2023)
Article
Dentistry, Oral Surgery & Medicine
Andre L. G. Moreira, Giselle A. Silva, Pedro H. F. Silva, Sergio L. Salvador, Raphael M. Vicente, Graziele C. Ferreira, Jose E. Tanus-Santos, Marcia P. A. Mayer, Karin H. Ishikawa, Sergio Luis Scombatti de Souza, Flavia A. C. Furlaneto, Michel R. Messora
Summary: This study found that Bifidobacterium animalis subspecies lactis HN019 can reduce the sequelae of experimental periodontitis in rats by modulating systemic parameters. Bifidobacterium lactis HN019 can decrease dyslipidemia, insulin resistance, proteinuria levels, systolic blood pressure, hepatic steatosis, and expression of adipokines, as well as improve intestinal microbiota imbalance and prevent alveolar bone loss. These findings highlight the importance of Bifidobacterium lactis HN019 in preventing the damage caused by periodontitis.
JOURNAL OF PERIODONTAL RESEARCH
(2023)
Article
Biotechnology & Applied Microbiology
Mateus Kawata Salgaco, Fellipe Lopes de Oliveira, Adilson Sartoratto, Victoria Mesa, Marcia Pinto Alves Mayer, Katia Sivieri
Summary: Type 2 diabetes is associated with dysbiosis in the gut, which can cause inflammation throughout the body. Probiotic use, specifically Lactobacillus acidophilus-La5, was shown to positively impact the gut microbiome of individuals with type 2 diabetes, leading to potential improvements in glycemic control.
FERMENTATION-BASEL
(2023)
Article
Multidisciplinary Sciences
Carolina Martins Nascimento, Mateus Campos Casaro, Evelyn Roxana Perez, Willian Rodrigues Ribeiro, Marcia Pinto Alves Mayer, Karin Hitomi Ishikawa, Adriana Lino-dos-Santos-Franco, Joice Naiara Bertaglia Pereira, Caroline Marcantonio Ferreira
Summary: Our study investigated the influence of OVA-induced allergic airway inflammation on gut homeostasis, and found that challenges in the respiratory system not only resulted in allergic airway inflammation but also in the loss of gut homeostasis.