Article
Biochemistry & Molecular Biology
Mafalda S. L. Aresta Branco, Alejandro Gutierrez Cruz, Lauren E. Peri, Violeta N. Mutafova-Yambolieva
Summary: During bladder filling, the urothelium releases ATP into the lamina propria, which activates P2X receptors and triggers the micturition reflex. The release of ATP is regulated by PANX1 channel and P2X7 receptor. PANX1 hinders the release of soluble ectonucleotidases, while P2X7 activation facilitates their release.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Cora L. Alvarez, Arnaud Chene, Jean-Philippe Semblat, Benoit Gamain, Claudine Lapoumeroulie, Claudio M. Fader, Claude Hattab, Jean Sevigny, Maria Florencia Leal Denis, Natalia Lauri, Mariano A. Ostuni, Pablo J. Schwarzbaum
Summary: The pathological processes of malaria patients are primarily due to metabolic and structural changes caused by Plasmodium falciparum, while uninfected RBCs and MPs play important roles in the regulation of extracellular ATP, activating ATP release and hydrolyzing eATP.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2022)
Article
Biochemistry & Molecular Biology
J. Schachter, C. L. Alvarez, Z. Bazzi, M. P. Faillace, G. Corradi, C. Hattab, D. E. Rinaldi, R. Gonzalez-Lebrero, M. Pucci Molineris, J. Sevigny, M. A. Ostuni, P. J. Schwarzbaum
Summary: In this study, the expression, localization and activities of ectonucleotidases in Caco-2 cells were investigated, showing their role in regulating extracellular ATP metabolism. The dynamic balance between intracellular ATP release and ectoATPase activity contributes to the regulation of eATP concentration. The data-driven algorithm helped quantify and predict rates of ATP release and consumption contributing to the dynamic accumulation of ATP at the cell surface.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2021)
Article
Biochemistry & Molecular Biology
Luiz Eduardo Baggio Savio, Raissa Leite-Aguiar, Vinicius Santos Alves, Robson Coutinho-Silva, Angela T. S. Wyse
Summary: Purinergic signaling is a crucial cell communication pathway mediated by extracellular nucleotides and nucleosides. Activation of P2 receptors influences the redox balance of cells, while enzymes like E-NTPDase1/CD39 and ecto-5'-nucleotidase/CD73 play key roles in regulating this process.
Article
Cell Biology
Maria Luiza Mukai Franciosi, Millena Daher Medeiros Lima, Maria Rosa Chitolina Schetinger, Andreia Machado Cardoso
Summary: This review explores the potential role of purinergic signaling in COVID-19, focusing on purinergic receptors and components such as ATP, CD39, and CD73. It suggests therapeutic combination or association possibilities targeting purinergic system components as a useful tool for future research, aiming to unveil a novel option to treat COVID-19 patients.
MOLECULAR AND CELLULAR BIOCHEMISTRY
(2021)
Review
Medicine, Research & Experimental
Karine Paula Reichert, Milagros Fanny Vera Castro, Charles Elias Assmann, Nathieli Bianchin Bottari, Vanessa Valeria Miron, Andreia Cardoso, Naiara Stefanello, Vera Maria Melchiors Morsch, Maria Rosa Chitolina Schetinger
Summary: This review focuses on the role of the purinergic system in diabetes and hypertension, highlighting changes in the expression of P1 and P2 receptors, as well as the activities of CD39, CD73, and ADA. It suggests that manipulating the purinergic axis at various levels could potentially prevent or exacerbate the development of diabetes and hypertension as a compensatory mechanism.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Cell Biology
Desiree Forstner, Jacqueline Guettler, Beatrice A. Brugger, Freya Lyssy, Lena Neuper, Christine Daxboeck, Gerhard Cvirn, Julia Fuchs, Kristin Kraeker, Alina Frolova, Daniela S. Valdes, Christina Stern, Birgit Hirschmugl, Herbert Fluhr, Christian Wadsack, Berthold Huppertz, Olivia Nonn, Florian Herse, Martin Gauster
Summary: Tissue insults in response to inflammation, hypoxia and ischemia cause the release of ATP, which modulates various pathological processes. CD39 and CD73 are two major enzymes that convert extracellular ATP into adenosine. This study investigated the expression of CD39 and CD73 in placental tissue and their regulation in response to platelet-derived factors and oxygen conditions. The results showed that placental CD39 expression increased in preeclampsia and platelet-derived factors led to deregulated CD39 expression. CD39 overexpression decreased extracellular ATP levels and abolished the upregulation of pro-inflammatory cytokine interleukin-1 beta. These findings suggest that increased placental CD39 could be an important anti-coagulant defense mechanism of the placenta.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Chemistry, Medicinal
Carla Fernanda Furtado Gardani, Eduardo Luiz Pedrazza, Victoria Santos Paz, Gabriele Goulart Zanirati, Jaderson Costa da Costa, Roberta Andrejew, Henning Ulrich, Juliete Nathali Scholl, Fabricio Figueiro, Liliana Rockenbach, Fernanda Bueno Morrone
Summary: This study investigated the expression of CD39 and CD73 enzymes in prostate cancer and their potential as therapeutic targets. The results showed higher levels of CD39 expression compared to CD73 in tissue samples and a correlation with Gleason score. In blood samples, CD39 expression in extracellular vesicles was significantly increased and positively correlated with ADP hydrolysis and Gleason score.
Article
Nanoscience & Nanotechnology
Won Ki Son, Yun Sik Choi, Young Woo Han, Dong Wook Shin, Kyunghun Min, Jiyoung Shin, Min Jeong Lee, Hokyoung Son, Dae Hong Jeong, Seon-Yeong Kwak
Summary: When plants are stressed, they release molecules to activate their defense system. Detecting these molecules can help address stress conditions and prevent the development of diseases. In this study, a non-destructive surface-enhanced Raman scattering (SERS)-based nanoprobe is developed for real-time detection of stress-related molecules in living plants. The nanoprobe, placed in the intercellular space, consists of a Si nanosphere surrounded by a corrugated Ag shell modified by a water-soluble cationic polymer. The nanosensors successfully monitor stress-related molecules in different plants, indicating the possible onset of a plant disease. Plasmonic nanosensor platforms could enable early diagnosis of stress and contribute to timely disease management in plants.
NATURE NANOTECHNOLOGY
(2023)
Review
Pharmacology & Pharmacy
Rui Pinto-Cardoso, Catarina Bessa-Andres, Paulo Correia-de-Sa, Jose Bernardo Noronha-Matos
Summary: The osteochondral unit consists of articular cartilage, subchondral bone, and calcified cartilage. Cells within this unit, such as chondrocytes and osteoblasts, release nucleotides to the local environment, which plays a role in tissue homeostasis. Hypoxic conditions can affect the expression and activity of purinergic signaling molecules, and the interplay between hypoxia and the purinergic signaling cascade contributes to osteochondral unit homeostasis. Understanding this relationship may lead to new therapeutic targets for osteochondral rehabilitation.
BIOCHEMICAL PHARMACOLOGY
(2023)
Review
Pharmacology & Pharmacy
Marta Lombardi, Martina Gabrielli, Elena Adinolfi, Claudia Verderio
Summary: ATP serves as a danger signal in the immune response by activating P2X and P2Y receptors to promote inflammation, with the P2X7 receptor being a critical player. ATP stimulation leads to cell proliferation, inflammasome activation, and extracellular vesicle release, which can transfer molecules to target cells. This review discusses the role of ATP in the biogenesis and dynamics of extracellular vesicles, highlighting their important functions in physiology and pathophysiology.
FRONTIERS IN PHARMACOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Srdjan M. M. Vlajkovic, Peter R. R. Thorne
Summary: The mammalian cochlea, as the sensory organ for hearing, is regulated by ATP release and adenosine signaling, triggering various physiological responses and playing important roles in cochlear development, homeostasis, and sensory transduction. Understanding these mechanisms may provide therapeutic strategies for cochlear rescue and prevention of hearing loss.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Alejandro Gutierrez Cruz, Mafalda S. L. Aresta Branco, Mahsa Borhani Peikani, Violeta N. Mutafova-Yambolieva
Summary: The bladder urothelium and suburothelium/lamina propria play important roles in sensory and transducer functions, with the involvement of afferent neurons and urothelium-derived purine mediators. The effective concentrations of purines at receptor targets depend on the degradation of ATP by ectonucleotidases. This study explores the interactions between peptidergic and purinergic signaling mechanisms in the bladder and suggests that they have an impact on bladder excitability and functions by regulating the concentrations of adenine purines at effector cells in the lamina propria.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Yuta Ryoden, Shigekazu Nagata
Summary: Extracellular ATP released from necrotic cells in inflamed tissues can activate the P2X7 receptor, leading to phospholipid scrambling mediated by the XK-VPS13A complex and subsequent cell death. P2X7 plays a crucial role in the activation of the immune system, while mutations in XK or VPS13A can disrupt homeostasis in the nervous and red blood cell systems.
Article
Biochemistry & Molecular Biology
Mafalda S. L. Aresta Branco, Alejandro Gutierrez Cruz, Mahsa Borhani Peikani, Violeta N. Mutafova-Yambolieva
Summary: The urinary bladder requires proper concentrations of extracellular ATP and other purines at receptor sites for normal functioning. Ectonucleotidases play a crucial role in dephosphorylation of ATP to ADP, AMP and ADO. The activation of metabolic pathways can restrict the release of ectonucleotidases and prevent excessive hydrolysis of ATP.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Pharmacology & Pharmacy
Ettel Keifman, Irene Ruiz-DeDiego, Diego Esteban Pafundo, Rodrigo Manuel Paz, Oscar Solis, Mario Gustavo Murer, Rosario Moratalla
BRITISH JOURNAL OF PHARMACOLOGY
(2019)
Article
Neurosciences
Rodrigo J. Alvarez, Diego E. Pafundo, Camila L. Zold, Juan E. Belforte
JOURNAL OF NEUROSCIENCE
(2020)
Article
Developmental Biology
Luis Manuel Munoz-Nava, Hugo Ariel Alvarez, Marycruz Flores-Flores, Osvaldo Chara, Marcos Nahmad
DEVELOPMENTAL BIOLOGY
(2020)
Article
Cell Biology
Alejandro Pizzoni, Zaher Bazzi, Gisela Di Giusto, Cora L. Alvarez, Valeria Rivarola, Claudia Capurro, Pablo J. Schwarzbaum, Paula Ford
Summary: The study suggests that AQP2 modulates Ca(2+) and ATP differently, which could explain the increased cell migration by AQP2.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Psychiatry
Diego E. Pafundo, Carlos A. Pretell Annan, Nicolas M. Fulginiti, Juan E. Belforte
Summary: The selective ablation of NMDA receptors in cortical and hippocampal interneurons during early postnatal development leads to an excitatory/inhibitory (E/I) imbalance in vivo in a mouse model relevant to schizophrenia. This imbalance is dependent on the cortical state and emerges during theta and gamma oscillations in the network.
SCHIZOPHRENIA BULLETIN
(2021)
Article
Biology
Emanuel Cura Costa, Leo Otsuki, Aida Rodrigo Albors, Elly M. Tanaka, Osvaldo Chara
Summary: Through a combination of modeling and experimental data, it was found that the regenerative response in Axolotls is associated with a specific signal that guides ependymal cell proliferation around the injury site; visualization of cell cycles using FUCCI technology revealed synchrony between cells, contributing to a better understanding of spinal cord regeneration.
Article
Anatomy & Morphology
Camilo Riquelme-Guzman, Maritta Schuez, Alexander Boehm, Dunja Knapp, Sandra Edwards-Jorquera, Alberto S. Ceccarelli, Osvaldo Chara, Martina Rauner, Tatiana Sandoval-Guzman
Summary: The axolotl is a key model for studying appendicular regeneration. Bone maturation in axolotls is a continuous process that extends throughout their life, with ossification of the appendicular bones being slow and continuing until complete.
DEVELOPMENTAL DYNAMICS
(2022)
Article
Dermatology
Nicole Berndt, Christine Wolf, Kristina Fischer, Emanuel Cura Costa, Peter Knuschke, Nick Zimmermann, Franziska Schmidt, Martin Merkel, Osvaldo Chara, Min Ae Lee-Kirsch, Claudia Gunther
Summary: The exonuclease TREX1 plays a protective role in preventing DNA accumulation and innate immune activation. TREX1 mutations lead to chronic DNA damage, IFN-1 response, and associated diseases such as Aicardi-Goutières syndrome, familial chilblain lupus, and systemic lupus erythematosus. Patients with lupus and TREX1 mutation show enhanced photosensitivity and increased levels of DNA damage in response to UV radiation, which can trigger disease flares.
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2022)
Article
Biochemistry & Molecular Biology
J. Schachter, C. L. Alvarez, Z. Bazzi, M. P. Faillace, G. Corradi, C. Hattab, D. E. Rinaldi, R. Gonzalez-Lebrero, M. Pucci Molineris, J. Sevigny, M. A. Ostuni, P. J. Schwarzbaum
Summary: In this study, the expression, localization and activities of ectonucleotidases in Caco-2 cells were investigated, showing their role in regulating extracellular ATP metabolism. The dynamic balance between intracellular ATP release and ectoATPase activity contributes to the regulation of eATP concentration. The data-driven algorithm helped quantify and predict rates of ATP release and consumption contributing to the dynamic accumulation of ATP at the cell surface.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2021)
Article
Multidisciplinary Sciences
Alberto S. Ceccarelli, Augusto Borges, Osvaldo Chara
Summary: The study investigates the effect of finite tissue size on model-predicted spatio-temporal morphogen distributions using an analytical approach. The results reveal a crossover tissue size, below which the infinite-domain model fails but the finite-domain model accurately describes the morphogen distribution. Additionally, the study finds that the infinite-domain model overestimates diffusion coefficients when fitting to finite spatial profiles.
ROYAL SOCIETY OPEN SCIENCE
(2022)
Article
Neurosciences
Tomas E. Sachella, Marina R. Ihidoype, Christophe D. Proulx, Diego E. Pafundo, Jorge H. Medina, Pablo Mendez, Joaquin Piriz
Summary: Fear is a pathological condition that can be studied using fear conditioning, which is the best-understood model of fear learning. This study investigates the role of the lateral habenula in fear conditioning and finds that it is involved in the formation of independent contextual and cued fear memories.
NEUROPSYCHOPHARMACOLOGY
(2022)
Article
Multidisciplinary Sciences
Catarina R. Oliveira, Dunja Knapp, Ahmed Elewa, Tobias Gerber, Sandra G. Gonzalez Malagon, Phillip B. Gates, Hannah E. Walters, Andreas Petzold, Hernan Arce, Rodrigo C. Cordoba, Elaiyaraja Subramanian, Osvaldo Chara, Elly M. Tanaka, Andras Simon, Maximina H. Yun
Summary: Through single-cell RNAseq analysis, Tig1 has been identified as a central cell surface determinant of proximal identity in salamander limb regeneration. Its overexpression leads to regeneration defects in the distal elements and elicits proximal displacement of blastema cells, while its neutralisation blocks proximo-distal cell surface interactions.
NATURE COMMUNICATIONS
(2022)
Correction
Multidisciplinary Sciences
Catarina R. Oliveira, Dunja Knapp, Ahmed Elewa, Tobias Gerber, Sandra G. Gonzalez Malagon, Phillip B. Gates, Hannah E. Walters, Andreas Petzold, Hernan Arce, Rodrigo C. Cordoba, Elaiyaraja Subramanian, Osvaldo Chara, Elly M. Tanaka, Andras Simon, Maximina H. Yun
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Juan M. F. Fernandez, Damian N. Spagnuolo, Maria T. Politi, Ivan A. Tello Santacruz, Miguel Schiavone, Cesar Caceres Monie, Horacio A. Avaca, Osvaldo Chara
Summary: Alteration of muscle activation sequence is a key mechanism in heart failure, and the lack of precise dyssynchrony quantification limits the success of cardiac resynchronization therapy (CRT). In this study, a computational pipeline was implemented to assess ventricular dyssynchrony by reconstructing vectorcardiograms from electrocardiograms. The dyssynchrony index showed significant differences between healthy controls and patients with left bundle branch block or pacemakers. Additionally, high dyssynchrony indexes were associated with mechanical dyssynchrony. This index shows promise in enhancing the selection and optimization of candidates for CRT.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Sarah Rouge, Sandrine Genetet, Maria Florencia Leal Denis, Michael Dussiot, Pablo Julio Schwarzbaum, Mariano Anibal Ostuni, Isabelle Mouro-Chanteloup
Summary: The study reveals that stomatin plays a major role in modulating PANX1 activity in red blood cells and its absence leads to significant defects in PANX1 function.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)