期刊
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
卷 206, 期 -, 页码 39-44出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijfoodmicro.2015.04.017
关键词
Vibrio species; Peptide nucleic acid; Fluorescent in situ hybridization
资金
- General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China [20131IK189]
- Inspection and Quarantine Technology Innovation Service Platform for Border Safety of Zhejiang Province [2006C17014]
- National Natural Science Foundation of China [31301492]
A peptide nucleic acid fluorescence in situ hybridization (PNA-FISH) method was developed for specific detection of the Vibrio genus. In silico analysis by BLAST and ProbeCheck showed that the designed PNA probe targeting the 16S rRNAs was suitable for specific identification of Vibrio. Specificity and sensitivity of the probe Vib-16S-1 were experimentally verified by its reactivity against 18 strains of 9 Vibrio species and 14 non-Vibrio strains of 14 representative species. The PNA FISH assay was able to identify 47 Vibrio positive samples from selectively enriched cultures of 510 samples of aquatic products and environments, comparable with the results obtained by biochemical identification and real-time PCR We conclude that PNA FISH can be an alternative method for rapid identification of Vibrio species in a broad spectrum of seafood or related samples. (C) 2015 Elsevier B.V. All rights reserved.
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