期刊
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
卷 211, 期 -, 页码 101-108出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijfoodmicro.2015.07.013
关键词
Human norovirus; Fresh produce; Attachment; Localization; Tulane virus; Murine norovirus
资金
- special emphasis grant from the National Integrated Food Safety Initiative [201001498]
- food safety challenge grant from the Agriculture and Food Research Initiative [2011-68003-30005]
- NoroCORE project grant of the US Department of Agriculture [2011-0494-13]
Fresh produce is a high risk food for human norovirus (NoV) contamination. To help control this pathogen in fresh produce, a better understanding of the interaction of human NoV and fresh produce needs to be established. In this study the attachment of human NoV and animal caliciviruses (murine norovirus, MNV-1; Tulane virus, TV) to fresh produce was evaluated, using both visualization and viral enumeration techniques. It was found that a human NoV GII.4 strain attached efficiently to the Romaine lettuce leaves and roots and green onion shoots, and that washing with PBS or 200 ppm of chlorine removed less than 0.4 log of viral RNA copies from the tissues. In contrast, TV and MNV-1 bound more efficiently to Romaine lettuce leaves than to the roots, and simple washing removed less than 1 log of viruses from the lettuce leaves and 1-4 log PFU of viruses from roots. Subsequently, the location of virus particles in fresh produce was visualized using a fluorescence-based Quantum Dots (Q-Dots) assay and confocal microscopy. It was found that human NoV virus-like particles (VLPs), TV, and MNV-1 associated with the surface of Romaine lettuce and were found aggregating in and around the stomata. In green onions, human NoV VLPs were found between the cells of the epidermis and cell walls of both the shoots and roots. However, TV and MNV-1 were found to be covering the surface of the epidermal cells in both the shoots and roots of green onions. Collectively, these results demonstrate that (i) washing with 200 ppm chlorine is ineffective in removing human NoV from fresh produce; and (ii) different viruses vary in their localization patterns to different varieties of fresh produce (C) 2015 Elsevier B.V. All rights reserved.
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