Article
Biochemistry & Molecular Biology
Sameh Samir Ali, Eman Elgibally, Maha A. Khalil, Jianzhong Sun, Abd El-Raheem R. El-Shanshoury
Summary: This study extracted an exopolysaccharide (EPS) from Azotobacter salinestris AZ-6, isolated from soil cultivated with leguminous plants. The EPS showed potential application value in the food industry and pharmaceutical applications due to its cytotoxicity against the MCF-7 tumor cell line, moderate cytotoxicity towards HepG-2 cell line, and potent antioxidant and antibacterial properties.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemical Research Methods
Jia Wang, David R. Salem, Rajesh K. Sani
Summary: The study investigated the production, characterization, and bioactivities of exopolysaccharides (EPSs) from a thermophilic bacterium Geobacillus sp. strain WSUCF1, resulting in two purified EPSs with potential applications in biomedical fields such as drug delivery carriers. EPS-1 and EPS-2 demonstrated high degradation temperatures and antioxidant activities, showing promising prospects for further exploitation.
Article
Biochemistry & Molecular Biology
Jinyu Zhu, Zhongfang Tan, Zhong Zhang, Xianming Shi
Summary: This study investigated the chemical structure, physicochemical properties, antioxidant capacity, antibacterial ability, and anti-biofilm formation activity of an exopolysaccharide (EPS) produced by Lactobacillus curvatus SJTUF 62116. The results showed that EPS-1 is mainly composed of glucose and mannose, and exhibits strong antioxidant and antibacterial properties.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Biochemistry & Molecular Biology
Thanapon Charoenwongpaiboon, Karan Wangpaiboon, Prapasri Septham, Nittaya Jiamvoraphong, Surapol Issaragrisil, Rath Pichyangkura, Chanchao Lorthongpanich
Summary: This study investigates the physicochemical properties and bioactivity of levan synthesized by recombinant levansucrase. The use of ultrasonication alters the physical and chemical properties of levan, enhancing its antiproliferative activity and Toll-like receptor expression. Sonicated levan shows potential for the development of cancer therapies.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Chemistry, Applied
Giulia Bisson, Clara Comuzzi, Elisa Giordani, Denise Poletti, Marta Boaro, Marilena Marino
Summary: This study characterized the chemical and biological properties of an exopolysaccharide EPS_B3 produced by Leuconostoc mesenteroides strain isolated from an Italian cheese. The EPS_B3 displayed antimicrobial, antibiofilm, bifidogenic, antioxidant, and DNA-protective activities. It showed potential applications in functional foods, especially for its antimicrobial activity and ability to improve the viability of bifidobacteria in probiotic foods. Further research is needed to assess its ability to reach the gastrointestinal tract intact.
CARBOHYDRATE POLYMERS
(2023)
Article
Chemistry, Applied
Li-Na Zhao, Yu-Biao Cao, Qin Luo, Yong-Liang Xu, Na Li, Cheng-Xiao Wang, Jun-Wei Xu
Summary: In this study, a novel approach to enhance the production and bioactivities of Ganoderma exopolysaccharides was explored. The PMM1 gene was cloned and overexpressed in Ganoderma for the first time, leading to a significant increase in exopolysaccharide production. The transformed strain also exhibited stronger regulation effects on macrophage.
CARBOHYDRATE POLYMERS
(2022)
Article
Food Science & Technology
Min Zhang, Tiantian Lai, Mengke Yao, Man Zhang, Zhennai Yang
Summary: The study found interactions between EPS produced by LAB and casein, resulting in improved physical properties and bioactivities of their complex. These interaction mechanisms are important for enhancing the functionality of fermented dairy products.
Article
Biochemistry & Molecular Biology
Maosheng Wei, Lihua Geng, Qingchi Wang, Yang Yue, Jing Wang, Ning Wu, Xiaoqing Wang, Chaomin Sun, Quanbin Zhang
Summary: Crude exopolysaccharides from the marine bacterium Bacillus sp. H5 were fractionated, leading to the isolation of a high molecular weight fraction EPS5SH. Further experiments showed that EPS5SH possessed immunostimulatory properties in murine macrophage cells by modulating the NF-kappa B and MAPK signaling pathways.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biochemistry & Molecular Biology
Xiaowan Feng, Hui Zhang, Phoency F. H. Lai, Zhiqiang Xiong, Lianzhong Ai
Summary: A pyruvated exopolysaccharide named LPE-1 was isolated and characterized from the fermentation broth of Lactobacillus plantarum AR307. LPE-1 was found to contain Galp and Glcp at a molar ratio of 2:1, with a molecular weight of 605 kDa. The presence of pyruvate group in LPE-1 plays a significant role in improving the viscosity and plasticity of dairy products.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Review
Agriculture, Multidisciplinary
Xuebo Li, Fuchen Jiang, Meiyan Liu, Yan Qu, Zhiqiong Lan, Xiaolin Dai, Chi Huang, Xuan Yue, Shiyi Zhao, Xiaoli Pan, Chen Zhang
Summary: Natural polysaccharides are important in various fields due to their diverse physical properties and biological activities. However, their bioactivities may be unsatisfactory, thus requiring structural modifications. Complexes of polysaccharides with metal ions have been reported to enhance their bioactivities and open up new applications.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Yuliana Vazquez-Gonzalez, Cristina Prieto, Milan Stojanovic, Cristiana A. V. Torres, Filomena Freitas, Juan Arturo Ragazzo-Sanchez, Montserrat Calderon-Santoyo, Jose M. Lagaron
Summary: The electrospinnability of FucoPol, a bacterial exopolysaccharide, was studied for the first time. The fibers obtained from pure FucoPol were not stable, but nanofibers were produced when blended with PEO and pullulan. FucoPol-based systems showed promising potential for various applications, such as packaging, agriculture, biomedicine, pharmacy, and cosmetics.
Article
Food Science & Technology
Yan Dai, Lei Wang, Xingyi Chen, Angxin Song, Laping He, Lingyuan Wang, Diandian Huang
Summary: This study investigated the extraction, emulsifying properties, and biological activities of polysaccharides from Lentinula edodes Sing (LES). The results showed that LES polysaccharides extracted by hot water had the best emulsifying capacity, while the ones extracted by 90 min ultrasonication exhibited the strongest antioxidant activities. Therefore, Lentinula edodes Sing polysaccharides may have potential applications as emulsifying agents in the food industry.
Article
Food Science & Technology
Kaiying Jia, Min Wei, Yao He, Yujie Wang, Hua Wei, Xueying Tao
Summary: Exopolysaccharide (EPS) from probiotic Enterococcus hirae WEHI01 was isolated and purified. The EPS consists of four fractions, I01-1, I01-2, I01-3, and I01-4, with I01-2 and I01-4 being the main components. The study found that I01-2 and I01-4 could enhance macrophage-mediated immune responses, indicating their potential application in functional food and medication production.
Article
Chemistry, Applied
Xavier Alvarez, Andreia Alves, Maximiano P. Ribeiro, Massimo Lazzari, Paula Coutinho, Ana Otero
Summary: Exopolysaccharides (EPS) produced by cyanobacteria have potential biomedical applications, such as new wound dressings. Addition of acetate ions to the culture medium can stimulate EPS production, leading to gel formation and promotion of fibroblast migration and proliferation. These Nostoc EPS hydrogels show promising properties for skin injury treatment.
CARBOHYDRATE POLYMERS
(2021)
Article
Plant Sciences
Lucas G. Castellani, Abril Luchetti, Juliet F. Nilsson, Julieta Perez-Gimenez, Caren Wegener, Andreas Schluter, Alfred Puhler, Antonio Lagares, Susana Brom, Mariano Pistorio, Karsten Niehaus, Gonzalo A. Torres Tejerizo
Summary: Biological nitrogen fixation is a major source of nitrogen into the biosphere, achieved through symbiosis between rhizobia and leguminous plants. Exopolysaccharides play a crucial role in establishing effective symbiosis between alfalfa and Ensifer meliloti. Rhizobium favelukesii LPU83 can infect alfalfa but lacks efficient nitrogen fixation, with different EPS biosynthesis gene organization compared to E. meliloti.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Wei-Han Hui, Yen-Lin Chen, Shu-Wei Chang
Summary: Crosslinking affects the mechanical behavior and tissue metabolism of collagen-based tissues. Aging and diabetes alter the crosslinking type and density, thus changing tissue properties. High-connectivity enzymatic crosslinking and advanced-glycation end-products result in uniform deformation under daily activity, while low-connectivity enzymatic crosslinking does not. High-connectivity model displays more sliding, while AGEs induce instability in structures near the binding sites.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Yao-Bin Ma, Xiao-Qiu Zhou, Wei-Dan Jiang, Pei Wu, Yang Liu, Shu-Wei Li, Ling Tang, Lu Zhang, Hai-Feng Mi, Lin Feng
Summary: Tea polyphenols (TPs) are an inexpensive feed additive that can protect the gill function of fish against Flavobacterium columnare (F. columnare) through various mechanisms, including suppression of oxidative stress, apoptosis, and inflammation.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Yan-Jia Guo, Jing-Ru Pang, Yu Zhang, Zhong-Rui Li, Xiao-Lin Zi, Hong-Min Liu, Ning Wang, Li-Juan Zhao, Ya Gao, Bo Wang, Piet Herdewijn, Cheng-Yun Jin, Ying Liu, Yi-Chao Zheng
Summary: This study identifies the conjugation of NEDD8 to LSD1 and its impact on GC cells, revealing the significance of LSD1 neddylation in GC.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Biochemistry & Molecular Biology
Geum-Jae Jeong, Fazlurrahman Khan, Nazia Tabassum, Young-Mog Kim
Summary: Vibrio species are motile gram-negative bacteria commonly found in aquatic environments. They can adapt to environmental fluctuations and develop protective mechanisms against host immune systems. Different types of sigma and anti-sigma factors play important roles in regulating gene expression and virulence in Vibrio species.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Zahra Mohammadbagheri, Behrooz Movahedi, Shaghayegh Saeedi, Abbas Rahmati
Summary: In this study, a reusable biobased composite hydrogel was successfully synthesized and used for the removal of Tl(I) from aqueous solutions. The hydrogel exhibited high adsorption capacity and thermal stability, and the adsorption process was found to be thermodynamically spontaneous. The results demonstrated that the hydrogel can be reused multiple times without significant loss of its adsorption capacity.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Iqra Batool, Muhammad Imran, Ayesha Anwar, Farhan Ahmed Khan, Afrah E. Mohammed, Ashwag Shami, Hafiz Iqbal
Summary: Enzyme immobilization on ZnO/SnO2 and ZnO/SnO2/SA nanocomposites was studied, and the catalytic and biochemical characteristics of the immobilized enzyme were investigated. The results showed that ZnO/SnO2 and ZnO/SnO2/SA nanostructures are suitable carriers for enzyme immobilization.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Elias Mosaffa, Dhruvi Patel, Nasim Amiri Ramsheh, Rishikumar Indravadan Patel, Atanu Banerjee, Hossein Ghafuri
Summary: In this study, a unique 3D bead structure of adsorbent, composed of bacterial cellulose microfilaments reinforced chitosan adorned with melamine 2D plates, was synthesized for anionic dye removal. The adsorbent showed high specific surface area and abundant functional groups, resulting in exceptional adsorption capacity. The characteristics and optimal conditions of the adsorbent were assessed and analyzed.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Carolina Gismene, Jorge Enrique Hernandez Gonzalez, Marilia de Freitas Calmon, Andrey Fabricio Ziem Nascimento, Angela Rocio Nino Santisteban, Felipe Antunes Calil, Alana Della Torre da Silva, Paula Rahal, Rejane Maira Goes, Raghuvir Krishnaswamy Arni, Ricardo Barros Mariutti
Summary: Mammaliicoccus sciuri, a bacterium of clinical and veterinary relevance, expresses the virulence factor Exfoliative Toxin C (ExhC), which binds specifically to desmoglein-1 (Dsg1) and plays a role in tissue integrity. This study investigates the necrotic activity of ExhC and identifies a variant lacking necrotic potential but retaining enzymatic activity through mutagenesis experiments and crystal structure determination.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Bibhas K. Bhunia, Ashutosh Bandyopadhyay, Souradeep Dey, Biman B. Mandal
Summary: Intervertebral disc degeneration is a major cause of chronic low back pain and disability. Researchers have developed a bioactive silk-based hybrid hydrogel system functionalized with decellularized human Wharton's jelly extracellular matrix as bioactive cues. The hydrogels mimic the rheological and mechanical properties of the native nucleus pulposus tissue and support cell viability, proliferation, and tissue maturation.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Qun Zhang, Yonggan Yan, Zhao Li, Jing Du, Kai Zhang, Liguo Zhang, Ting Wang, Alberto Bianco, Shaohua Ge, Baojin Ma
Summary: A new crosslinking strategy has been devised to construct injectable sodium alginate hydrogels, which can be regulated for injectability and have great potential in biomedical applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Seong Eun Hong, Ji-Soo Lee, Hyeon Gyu Lee
Summary: The potential of multi-layer nanoemulsions for improving the cellular uptake, antioxidant activity, and in vitro bioaccessibility of alpha-tocopherol was examined. The results showed that the multi-layer nanoemulsions significantly enhanced the cellular uptake and antioxidant activity of alpha-tocopherol compared to free coumarin 6. Furthermore, the use of chitosan and dextran sulfate as wall materials in the multi-layer nanoemulsions led to even higher cellular uptake, in vitro bioaccessibility, and antioxidant activity of alpha-tocopherol.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Jingjing Yu, Yanhui Zhang, Ruoning Zhang, Yanxiang Gao, Like Mao
Summary: High internal phase emulsions (HIPEs) based on beeswax (BW) oleogels and octenyl succinic acid starch (OSA starch) were prepared by a simple one-step method. The results showed that oleogelation improved the stability and encapsulation capability of the HIPEs, which is significant for the development of novel healthy food.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Biochemistry & Molecular Biology
Bi-Feng Yao, Xiu-Ju Luo, Jun Peng
Summary: The balanced and continuous fission and fusion of mitochondria, regulated by OPA1, are important for maintaining mitochondrial dynamics in cardiovascular disorders. Understanding the role of OPA1 in mitochondrial fusion can provide insights into the pathogenesis of cardiovascular diseases.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Biochemistry & Molecular Biology
Gokul Paramasivam, Anandhavelu Sanmugam, Vishnu Vardhan Palem, Murugan Sevanan, Ananda Babu Sairam, Nachiappan Nachiappan, Buhyun Youn, Jung Sub Lee, Muthuchamy Nallal, Kang Hyun Park
Summary: Nanomaterials are being used to deliver therapeutic agents in living systems. Different nanotechnological applications such as immunosensors, immune assays, and drug delivery are being implemented using nanotechnology. Genomics plays a crucial role in selecting appropriate drugs for personalized cancer therapy. It helps in analyzing gene and protein expression, disease classification, and designing disease models. Delivering drugs to specific areas using nanoparticles is a challenging issue.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Jing Li, Wei Wang, Han Wu, Feng Peng, Hui Gao, Ying Guan
Summary: In this study, CMH-based CPF films were prepared and their thermal and barrier properties were successfully regulated. The tensile strength and water vapor barrier property of CPF films were significantly improved, making them promising for food packaging applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)