Article
Biochemistry & Molecular Biology
Gloria Pegoli, Marika Milan, Pierluigi Giuseppe Manti, Andrea Bianchi, Federica Lucini, Philina Santarelli, Claudia Bearzi, Roberto Rizzi, Chiara Lanzuolo
Summary: Knock-out of the Cdkn2a locus enhances the proliferative capability of stem cells in some tissues and response to severe physiological and non-physiological damages in different organs.
Review
Biochemistry & Molecular Biology
Alan Rawls, Bridget K. Diviak, Cameron I. Smith, Grant W. Severson, Sofia A. Acosta, Jeanne Wilson-Rawls
Summary: Muscular dystrophies are genetic muscle-wasting disorders characterized by chronic inflammation and fibrotic scarring in muscle tissue. Duchenne muscular dystrophy, the most common form, is typically treated with anti-inflammatory glucocorticoids; however, their long-term use is limited by adverse side effects. Developing new pharmacotherapeutic approaches to reduce muscle damage and promote repair is crucial.
Article
Cell Biology
Jia-Bin Yu, Dong-Sun Lee, Babu J. Padanilam, Jinu Kim
Summary: Cisplatin, a chemotherapeutic drug, can cause kidney damage. This study established a clinically relevant model and found that cisplatin can transform kidney fibroblasts into myofibroblasts through G2/M arrest and cellular senescence.
Article
Cell Biology
Fatima D. Elzamzami, Arushi Samal, Adith S. Arun, Tejas Dharmaraj, Neeti R. Prasad, Alex Rendon-Jonguitud, Lauren Devine, Jeremy D. Walston, Robert N. Cole, Katherine L. Wilson
Summary: This study found that chronic inflammation may disrupt the binding of wildtype lamin A/C with tissue-specific partners, leading to the occurrence of frailty. Multiple proteins associated with lamin A/C binding were identified, and four of them were found to be related to Emery-Dreifuss muscular dystrophy.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Medicine, General & Internal
Michal Marchel, Agnieszka Madej-Pilarczyk, Agata Tyminska, Roman Steckiewicz, Ewa Ostrowska, Julia Wysinska, Vincenzo Russo, Marcin Grabowski, Grzegorz Opolski
Summary: Atrial arrhythmias are common in patients with muscular dystrophy associated with EMD/LMNA mutations, but occur earlier in EMD patients. Ventricular arrhythmias are more common (60%) in LMNA and occur earlier compared to the EMD group.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Cell Biology
Lei Liu, Xianlin Yue, Zewei Sun, William S. Hambright, Qi Feng, Yan Cui, Johnny Huard, Paul D. Robbins, Zhihui Wang, Xiaodong Mu
Summary: This study reveals that senescent immune cells, such as macrophages, play an important role in the development of muscle dystrophy by impacting the function of muscle stem cells. Removing these senescent cells with fisetin can effectively improve the function of muscle stem cells and the phenotypes of dystrophic muscles.
Article
Oncology
Markus Kaller, Wenjing Shi, Heiko Hermeking
Summary: Loss of AP4 in breast cancer cells leads to DNA damage, senescence, and reduced proliferation. Deletion of p53 can reverse the senescence and proliferation defects in AP4-deficient cells. Loss of AP4 enhances the repression of DREAM and E2F target genes induced by c-MYC through p53.
Review
Genetics & Heredity
Rinka Nakajima, Lin Zhao, Yaxuan Zhou, Mashiro Shirasawa, Ayato Uchida, Hikaru Murakawa, Mariana Fikriyanti, Ritsuko Iwanaga, Andrew P. Bradford, Keigo Araki, Tomoko Warita, Kiyoshi Ohtani
Summary: Transcription factor E2F, the main target of the tumor suppressor pRB, plays essential roles in cell proliferation and tumor suppression. In almost all cancers, pRB is inactivated, leading to enhanced E2F activity. Trials have been conducted to suppress enhanced E2F activity for targeted cancer therapy, but it may also affect normal growing cells due to the shared mechanism of growth stimulation.
Article
Clinical Neurology
Giulio Gadaleta, Guido Urbano, Chiara Brusa, Rossella D'Alessandro, Enrica Rolle, Ilaria Cavallina, Alessio Mattei, Fulvia Ribolla, Claudia Raineri, Stefano Pidello, Liliana Vercelli, Federica S. Ricci, Tiziana E. Mongini
Summary: The clinical characteristics of adults with DMD include mechanical ventilation, swallowing and nutritional issues, and bone density alterations. Other issues include respiratory infections, gastrointestinal symptoms, metabolic acidosis, psychiatric symptoms, and chronic pain. Patients have a negative perception of their physical health but a more positive assessment of their mental health.
EUROPEAN JOURNAL OF NEUROLOGY
(2023)
Article
Critical Care Medicine
Juan Chen, Xin Yi Chen, Xiao Xia Cong, Shen Wang, Shui Bo Xu, Yu Ting Sun, Yi Ting Zhou, Li Ling Zheng, Man Huang
Summary: This study found that both weight and strength of muscles were significantly decreased in septic mice induced by cecum ligation and puncture or LPS. Inhibition of p53 expression reduced the number of senescent myoblasts, indicating that sepsis-induced cellular senescence is dependent on p53. Additionally, metformin attenuated muscle senescence and improved muscle strength in septic mice, suggesting it as a potential therapeutic drug for ICU-AW.
Review
Cell Biology
Lihuan Guan, Karen C. Crasta, Andrea B. Maier
Summary: This review highlights the significance of cellular senescence markers in human peripheral blood cells, summarizes related experiments and detection methods, as well as studies on demographic and clinical characteristics.
AGEING RESEARCH REVIEWS
(2022)
Article
Neurosciences
Nastasia Cardone, Valentina Taglietti, Serena Baratto, Kaouthar Kefi, Baptiste Periou, Ciryl Gitiaux, Christine Barnerias, Peggy Lafuste, France Leturcq Pharm, Juliette Nectoux Pharm, Chiara Panicucci, Isabelle Desguerre, Claudio Bruno, Francois-Jerome Authier, Chiara Fiorillo, Frederic Relaix, Edoardo Malfatti
Summary: In this study, we performed myopathologic analysis on muscle biopsies from DMD patients and found an increase in fibro-adipogenic progenitors, which contribute to fibrotic progression and lipid deposition. At the same time, there was a decline in muscle regenerative capacity, which was strongly correlated with compromised activation and expansion of muscle stem cells. Additionally, our study revealed an early acquisition of a senescence phenotype by DMD-afflicted muscle stem cells. These findings highlight the importance of muscle stem cell senescence as a pivotal readout for future therapeutic interventions.
ACTA NEUROPATHOLOGICA COMMUNICATIONS
(2023)
Article
Chemistry, Medicinal
Lizzia Raffaghello, Elisa Principi, Serena Baratto, Chiara Panicucci, Sara Pintus, Francesca Antonini, Genny Del Zotto, Andrea Benzi, Santina Bruzzone, Paolo Scudieri, Carlo Minetti, Elisabetta Gazzerro, Claudio Bruno
Summary: In this study, the therapeutic effectiveness of a selective P2X7 purinoreceptor antagonist, A438079, was evaluated in limb-girdle muscular dystrophy R3. The results showed that the P2X7 antagonist improved clinical parameters, reduced muscle inflammation and fibrosis, and upregulated immunosuppressive regulatory T cells.
Article
Orthopedics
Chang-chun Chen, Jing Chen, Wen-liang Wang, Liang Xie, Chuan-qiang Shao, Yan-xiang Zhang
Summary: The study found that SNPC-Exo treatment can induce NPC senescence, while inhibition of the P53/P21 pathway can attenuate SNPC-Exo-induced NPC senescence. Inhibition of the P53/P21 pathway led to an increase in NPC proliferation rate, a decrease in the percentage of SA-beta-gal-positive cells, and facilitated NPC entry into the S phase.
ORTHOPAEDIC SURGERY
(2021)
Review
Biochemistry & Molecular Biology
Kurt Engeland
Summary: RB and p53 are central tumor suppressors that play key roles in regulating the cell division cycle. RB downregulates numerous genes by forming complexes with E2F transcription factors, and its repression is released through phosphorylation. p53 activates CDKN1A gene to regulate RB phosphorylation, leading to expression of cell cycle regulators. There is an overlap between RB and DREAM signaling pathways.
CELL DEATH AND DIFFERENTIATION
(2022)
Article
Cell Biology
Neylen Del Toro, Ana Fernandez-Ruiz, Lian Mignacca, Paloma Kalegari, Marie-Camille Rowell, Sebastian Igelmann, Emmanuelle Saint-Germain, Mehdi Benfdil, Stephane Lopes-Paciencia, Lea Brakier-Gingras, Veronique Bourdeau, Gerardo Ferbeyre, Frederic Lessard
Article
Cell Biology
Mathieu Vernier, Catherine R. Dufour, Shawn McGuirk, Charlotte Scholtes, Xiaojing Li, Guillaume Bourmeau, Hellen Kuasne, Morag Park, Julie St-Pierre, Etienne Audet-Walsh, Vincent Giguere
GENES & DEVELOPMENT
(2020)
Review
Endocrinology & Metabolism
Mathieu Vernier, Vincent Giguere
Summary: Aging is a degenerative process caused by the accumulation of cellular and tissue lesions, with deregulated mitochondrial function being a central regulator. Research highlights the importance of the PGC-1/ERR axis in age-related mitochondrial deregulation and tissue dysfunction, suggesting pharmacological targeting of this axis may help alleviate the onset of aging and its adverse effects.
JOURNAL OF MOLECULAR ENDOCRINOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Mathieu Vernier, Shawn McGuirk, Catherine R. Dufour, Liangxinyi Wan, Etienne Audet-Walsh, Julie St-Pierre, Vincent Giguere
Review
Oncology
Olga Moiseeva, Jordan Guillon, Gerardo Ferbeyre
Summary: Senescence is a tumor suppressor response that prevents mutated cell proliferation and alerts the immune system, but can be impaired over time, promoting cancer progression. A polyploid barrier and nuclear lamina act as additional safeguards to prevent cancer development.
SEMINARS IN CANCER BIOLOGY
(2022)
Editorial Material
Cell Biology
Etienne Audet-Walsh, Mathieu Vernier, Benoit Viollet
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Xiaonan Liu, Yu Chai, Guanqiao Liu, Weiping Su, Qiaoyue Guo, Xiao Lv, Peisong Gao, Bin Yu, Gerardo Ferbeyre, Xu Cao, Mei Wan
Summary: The study reveals that glucocorticoids (GCs) disrupt bone development by interfering with osteoclast-secreted angiogenin (ANG), leading to vascular cell aging, impaired angiogenesis, and unbalanced skeletal development.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Audrey Hebert, Maxime Parisotto, Marie-Camille Rowell, Alexandra Dore, Ana Fernandez, Guillaume Lefrancois, Paloma Kalegari, Gerardo Ferbeyre, Andreea R. Schmitzer
Summary: Substituted biguanidium salts have shown promising ability to inhibit pancreatic cancer cell growth, with phenylethynyl biguanidium salts exhibiting superior activity in crossing hydrophobic barriers, enhancing mitochondrial accumulation, and anticancer effects. The most active compound, 1b, demonstrated similar mechanisms as metformin but at a much lower concentration, showing significant inhibition of pancreatic cancer xenograft growth in vivo. Existing clinical trial biguanides showed limited activity in comparison.
SCIENTIFIC REPORTS
(2021)
Review
Cardiac & Cardiovascular Systems
Mozhdeh Mehdizadeh, Martin Aguilar, Eric Thorin, Gerardo Ferbeyre, Stanley Nattel
Summary: This review discusses the role of cellular senescence in cardiac disease, outlines therapeutic strategies for targeting senescence, and considers potential implications for improving the management of patients with heart disease. The complex senescence-associated secretory phenotype (SASP) of senescent cells has important effects on cell and tissue biology. Future research is needed to better understand the precise role of senescent cells in various cardiac pathologies and develop strategies for preventing their accumulation.
NATURE REVIEWS CARDIOLOGY
(2022)
Article
Pharmacology & Pharmacy
Rachid Abaji, Vincent Roux, Ismahene Reguieg Yssaad, Paloma Kalegari, Vincent Gagne, Romain Gioia, Gerardo Ferbeyre, Christian Beausejour, Maja Krajinovic
Summary: The role of MYBBP1A gene and rs3809849 in pancreatic cancer and lymphoblastic leukemia, as well as their response to asparaginase treatment, were investigated. MYBBP1A was found to play an important role in regulating various cellular functions and its rs3809849 polymorphism was found to have a tissue-specific role in asparaginase treatment response.
Article
Cell Biology
Catherine R. Dufour, Charlotte Scholtes, Ming Yan, Yonghong Chen, Lingwei Han, Ting Li, Hui Xia, Qiyun Deng, Mathieu Vernier, Vincent Giguere
Summary: An increasing number of studies support the direct role of nuclear mTOR in gene regulation and chromatin structure. However, the limited knowledge of chromatin-bound mTOR partners restricts our understanding of how nuclear mTOR controls transcription. This study comprehensively maps the mTOR chromatin-bound interactome in prostate cancer cellular models and identifies a 67-protein interaction network enriched with chromatin modifiers, transcription factors, and SUMOylation machinery. SUMO2/3 and nuclear pore protein NUP210 are among the strongest interactors, while the androgen receptor (AR) is the dominant androgen-inducible mTOR partner. Further investigation reveals the role of NUP210 in mTOR nuclear trafficking, the functional transcriptional module formed by mTOR, AR, and the nucleosome remodeling and deacetylase (NuRD) complex, and the androgen-dependent mTOR-SUMO2/3 promoter-enhancer association.
Review
Oncology
Sebastian Igelmann, Frederic Lessard, Gerardo Ferbeyre
Summary: Emerging evidence suggests that the organization of cells into membraneless sub-compartments, known as biological condensates, confers unique properties to cancer cells. Recent research has shown that genetic and epigenetic changes can drive the formation of these condensates, leading to the stable maintenance of cellular states. This review highlights the specific biological condensates in cancer, initiated by mutant oncoproteins, RNA-binding proteins, or lincRNAs, which regulate oncogenic gene expression programs and cancer metabolism. Targeting these oncogenic condensates with effective anticancer drugs could be a promising therapeutic approach.
Article
Biochemistry & Molecular Biology
Mathieu Lussier-Price, Haytham M. Wahba, Xavier H. Mascle, Laurent Cappadocia, Veronique Bourdeau, Christina Gagnon, Sebastian Igelmann, Kazuyasu Sakaguchi, Gerardo Ferbeyre, James G. Omichinski
Summary: The N-terminal region of SUMO proteins plays an auto-inhibition role in promoting PML-NB formation, and this auto-inhibition can be relieved by zinc, leading to increased PML-NB formation within cells.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Oncology
Md Gulam Musawwir Khan, Nadia Boufaied, Mehdi Yeganeh, Rajani Kandhi, Stephanie Petkiewicz, Ankur Sharma, Akihiko Yoshimura, Gerardo Ferbeyre, David P. Labbe, Sheela Ramanathan, Subburaj Ilangumaran
Summary: The SOCS1 protein protects liver cells from becoming cancerous and its deficiency increases susceptibility to liver cancer. CDKN1A is a protein that can protect against liver cancer but under certain circumstances, it can promote cancer growth. Our findings show that SOCS1 suppresses the expression of CDKN1A, preventing liver cells from withstanding the stress associated with cancer growth. This pathway could be targeted for therapeutic strategies in SOCS1-deficient liver cancers.
Article
Oncology
Maxime Parisotto, Nhung Vuong-Robillard, Paloma Kalegari, Thulaj Meharwade, Loick Joumier, Sebastian Igelmann, Veronique Bourdeau, Marie-Camille Rowell, Michael Pollak, Mohan Malleshaiah, Andreea Schmitzer, Gerardo Ferbeyre
Summary: The use of metformin as a single antitumor agent has been disappointing, but combining it with the NAD biosynthesis inhibitor FK866 has shown superior antitumor activity. This study reveals the mechanism of metformin's antitumor actions and suggests that targeting mitochondria and NAD biosynthesis can lead to effective antitumor therapies.